• Genomics & Informatics
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• v.14 no.3
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• pp.104-111
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• 2016

Zika virus (ZIKV) is a mosquito borne pathogen, belongs to Flaviviridae family having a positive-sense single-stranded RNA genome, currently known for causing large epidemics in Brazil. Its infection can cause microcephaly, a serious birth defect during pregnancy. The recent outbreak of ZIKV in February 2016 in Brazil realized it as a major health risk, demands an enhanced surveillance and a need to develop novel drugs against ZIKV. Amodiaquine, prochlorperazine, quinacrine, and berberine are few promising drugs approved by Food and Drug Administration against dengue virus which also belong to Flaviviridae family. In this study, we performed molecular docking analysis of these drugs against nonstructural 3 (NS3) protein of ZIKV. The protease activity of NS3 is necessary for viral replication and its prohibition could be considered as a strategy for treatment of ZIKV infection. Amongst these four drugs, berberine has shown highest binding affinity of -5.8 kcal/mol and it is binding around the active site region of the receptor. Based on the properties of berberine, more similar compounds were retrieved from ZINC database and a structure-based virtual screening was carried out by AutoDock Vina in PyRx 0.8. Best 10 novel drug-like compounds were identified and amongst them ZINC53047591 (2-(benzylsulfanyl)-3-cyclohexyl-3H-spiro[benzo[h]quinazoline-5,1'-cyclopentan]-4(6H)-one) was found to interact with NS3 protein with binding energy of -7.1 kcal/mol and formed H-bonds with Ser135 and Asn152 amino acid residues. Observations made in this study may extend an assuring platform for developing anti-viral competitive inhibitors against ZIKV infection.

• Parasites, Hosts and Diseases
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• v.57 no.3
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• pp.283-290
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• 2019

A rapid diagnostic test (RDT) kit was developed to detect non-structural protein 1 (NS1) of yellow fever virus (YFV) using monoclonal antibody. NS1 protein was purified from the cultured YFV and used to immunize mice. Monoclonal antibody to NS1 was selected and conjugated with colloidal gold to produce the YFV NS1 RDT kit. The YFV RDTs were evaluated for sensitivity and specificity using positive and negative samples of monkeys from Brazil and negative human blood samples from Korea. Among monoclonal antibodies, clones 3A11 and 3B7 proved most sensitive, and used for YFV RDT kit. Diagnostic accuracy of YFV RDT was fairly high; Sensitivity was 0.0% and specificity was 100% against Dengue viruses type 2 and 3, Zika, Chikungunya and Mayaro viruses. This YFV RDT kit could be employed as a test of choice for point-of-care diagnosis and large scale surveys of YFV infection under clinical or field conditions in endemic areas and on the globe.

• Clinical and Experimental Pediatrics
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• v.62 no.7
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• pp.274-280
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• 2019

Purpose: To analyze the growth response to growth hormone (GH) therapy in prepubertal patients with Noonan syndrome (NS) harboring different genetic mutations. Methods: Twenty-three patients with prepubertal NS treated at Pusan National University Children's Hospital between March 2009 and July 2017 were enrolled. According to the disease-causing genes identified, the patients with NS were divided into 4 groups. Three groups were positive for mutations of the PTPN11, RAF1, and SOS1 genes. The five genes undetected (FGU) group was negative for PTPN11, RAF1, SOS1, KRAS, and BRAF gene mutations. The influence of genotype was retrospectively analyzed by comparing the growth parameters after GH therapy. Results: The mean chronological age at the start of GH treatment was $5.85{\pm}2.67years$. At the beginning of the GH treatment, the height standard deviation score (SDS), growth velocity (GV), and lower levels of insulin-like growth factor-1 (IGF)-1 levels were not statistically different among the groups. All the 23 NS patients had significantly increased height SDS and serum IGF-1 level during the 3 years of treatment. GV was highest during the first year of treatment. During the 3 years of GH therapy, the PTPN11, RAF1, and SOS1 groups showed less improvement in height SDS, IGF-1 SDS, and GV, and less increase in bone age-to-chronological age ratio than the FGU group. Conclusion: The 3-year GH therapy in the 23 prepubertal patients with NS was effective in improving height SDS, GV, and serum IGF-1 levels. The FGU group showed a better response to recombinant human GH therapy than the PTPN11, RAF1, and SOS1 groups.

• Proceedings of the IEEK Conference
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• 2004.06b
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• pp.367-370
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• 2004

We fabricated PLT(5) thin film on $Pt/TiO_x/SiO_2/Si$ substrate by using sol-gel method and investigated leakage current, switching and retention properties. The leakage current density of PLT(5) thin film was $3.56{\times}10^{-7}A/cm^2$ at 4V. In the examination of switching properties, pulse voltage and load resistance were $2V{\~}5V$ and $50{\Omega}{\~}3.3k{\Omega}$, respectively. Switching time had a tendency to decrease from 520ns to 140ns with the increase of pulse voltage, and also the time was increased from 140ns to $13.7{\mu}s$ with the increase of load resistance. The activation energy obtained from the relation of applied pulse voltage and switching time was about 143kV/cm. The error of switched charge density between hysteresis loop and experiment of polarization switching was about $10\%$. Also, polarization in retention was decreased as much as about $8\%$ after $10^5$s.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.35 no.5B
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• pp.762-770
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• 2010

The researches about energy efficieny of wireless sensor MAC protocol is an issue in present days. Therefore, MAC and routing protocols for reducing energy consumption at sensor nodes is needed. In this study, a low-power MAC protocol for sensor network is proposed, which in based on X-MAC by exploiting virtual synchronization. The virtual synchronization technique lets senders postpone packet transmission until receivers' wake-up time, so that senders transmit only one or two short preambles. Using NS-2, a proposed MAC protocol improve the energy efficiency by 10% compared with the X-MAC protocol.

• The KIPS Transactions:PartA
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• v.18A no.6
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• pp.281-292
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• 2011

In this paper, a simulator adapted in AMI network environment with NS-2 was designed and implemented. The limited AMI operation environment such as processing times of data, network protocol and system performance can be simulated to find out the optimal AMI formations. Consequently, it is simulated under conditions that are closely analogous to the actual networks and each device. In future, the result of simulation would be applied to AMI network, mitigated the waste of resources and much contributed towards the real optimal AMI deployments in utilities.

• Korean Journal of Optics and Photonics
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• v.6 no.2
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• pp.130-134
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• 1995

A small signal gain coefficient of Nd:glass was measured and a cavity-dumped laser was constructed. To measure the small signal gain coefficient, we constructed a resonator consisting of Nd:glass, Pockels cell, polarizing beam splitter. The measured small signal gain coefficient was $0.088 cm_{-1}$ when the input energy was 100 J and the round-trip internal loss of the resonator was 56%. The cavity-dumped laser was constructed using Nd:glass. 2 m radius of curvature HR-mirrors, Pockels cell, polarizinig beam splitter and $\lambda/4$ plate. The output energy of cavity-dumped laser was 0.85 J at 140 J input energy and the laser pulse width was 8 ns.s 8 ns.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2012.10a
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• pp.642-645
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• 2012

MANET has disadvantage for information intrusion caused from non-infra structure. That is based on mixed problems caused from terminal devices has no capability of various resources using abilities to run intrusion prevention function, and also from difficulty of no easy using infra structural server like as firewall. In this paper, transmission performances, be effected from information intrusion and IDS(Intrusion Detection System), are analyzed for large scale MANET, and weakness from information intrusion of MANET are studied. This study is for large scale MANET which has some large communication area and lots of nodes, voice traffic, based on VoIP protocols, is considered as application services be transmitted over MANETs. Computer simulation using NS-2 is used to measure and show MOS and call connection ratios.

• Food Science of Animal Resources
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• v.37 no.4
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• pp.529-534
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• 2017

Probiotics have been known to reduce high-fat diet (HFD)-induced metabolic diseases, such as obesity, insulin resistance, and type 2 diabetes. We recently observed that Lactobacillus acidophilus NS1 (LNS1), distinctly suppresses increase of blood glucose levels and insulin resistance in HFD-fed mice. In the present study, we demonstrated that oral administration of LNS1 with HFD feeding to mice significantly reduces hepatic expression of phosphoenolpyruvate carboxykinase (PEPCK), a key enzyme in gluconeogenesis which is highly increased by HFD feeding. This suppressive effect of LNS1 on hepatic expression of PEPCK was further confirmed in HepG2 cells by treatment of LNS1 conditioned media (LNS1-CM). LNS1-CM strongly and specifically inhibited $HNF4{\alpha}-induced$ PEPCK promoter activity in HepG2 cells without change of $HNF4{\alpha}$ mRNA levels. Together, these data demonstrate that LNS1 suppresses PEPCK expression in the liver by regulating $HNF4{\alpha}$ transcriptional activity, implicating its role as a preventive or therapeutic approach for metabolic diseases.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2002.11a
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• pp.306-310
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• 2002

Today, corporations are approach to inside information using public network such as Internet groping economical way to utilize public network to corporation information network without buying equipment. But, special quality of internet, Virtual Private Net (VPN) that it uses net that is observed as technology that can be guaranteed public safety division transmission and data securitybecause of can not secure data transmission. In this paper. add VPN function ONU&OLT of ATM-PON system and propose SCB (Single Copy Broadcasting). When there is VPN function to ATM network, the speed can be fast, and reduce rain track pick quantity during time more. Performance analysis network simulation that use NS-2.