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Effects of Concentrated Pig Slurry Using Membrane Filter on the Growth and Yield of Tomato in Nutriculture (막분리 돈분뇨 농축액비를 이용한 양액재배가 토마토의 생육과 수량에 미치는 영향)

  • Ryoo, Jong-Won;Seo, Woon-Kab
    • Journal of Animal Environmental Science
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    • v.14 no.2
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    • pp.119-128
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    • 2008
  • This experiment was conducted to investigate the effects of concentrated pig slurry using membrane filter on growth of tomato in nutriculture. Pig slurry was filtered by ultra filtration and concentrated by reverse osmosis process. Filtration of pig slurry was necessary to prevent the hose clogging in nutriculture. The concentrated pig slurry (CS) and nutrient solution (NS) were mixed by six different mixing ratios of 0:100, 20:80, 40:60, 60:40%, 80: 20 and 100%:0% based on nitrogen content. The chemical nutrient solution was the solution of National Horticulture Research Station for the growth of tomato. The concentration of nutrient solution was adjusted a range of $1.6{\sim}2.0mS/cm$ in EC. The plant height of tomato treated with CS 20+NS 80% was similar with NS 100% control plot. Plant height was highest in the plot of CS 20+NS 80%. The treatment of 100% concentrated pig slurry was lowest in the gowth characteristics of tomato. Number of cluster was very lower in 100% concentrated pig slurry compared with plot of chemical nutrient solution. In the beginning of growth stage, SPAD reading value was reduced in plot treated with CS 100%, but CS 20+NS 80% plot was higher compared to 100% concentrated pig slurry. SPAD value of tomato leaves was decreased as the amount of CS was increased. The SPAD value also in treatment of concentrated pig slurry was lower in the middle growth stage compared to control plot. The dry weight of stem and leaf were 107.4, 104.2g in plot of NS 100% and CS 20%+NS 80%, respectively. The fruit number and weight were decreased at high application plots of concentrated pig slurry, The fruit setting of tomato showed lowest in the plot treated with 100% concentrated pig slurry, and the growth of tomato severely decreased after application of 100% CS treatment. In conclusion, the growth characteristics such as plant height and fruit weight of tomato were not significantly different between the plots treated with mixture of 20% CS +80%NS and 100% nutrient solution treatment. In conclusion, the mixture solution of 20% of concentrated pig slurry and 80% of nutrient solution could be used as a nutrition solution of tomato nuticulture.

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Profiles of coagulase-positive and -negative staphylococci in retail pork: prevalence, antimicrobial resistance, enterotoxigenicity, and virulence factors

  • Lee, Gi Yong;Yang, Soo-Jin
    • Animal Bioscience
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    • v.34 no.4
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    • pp.734-742
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    • 2021
  • Objective: The present study aimed to investigate the occurrence and species of coagulase-positive staphylococci (CoPS) and coagulase-negative staphylococci (CoNS) in retail pork meat samples collected during nationwide monitoring. The staphylococcal isolates were characterized for antimicrobial and zinc chloride resistance and enterotoxigenic potential. Methods: A total of 260 pre-packaged pork meat samples were collected from 35 retail markets in 8 provinces in Korea for isolation of staphylococci. Antimicrobial and zinc chloride resistance phenotypes, and genes associated with the resistance phenotypes were determined on the isolates. Furthermore, the presence and distribution of 19 staphylococcal enterotoxin (SE) genes and enterotoxin-like genes among the pork-associated staphylococci were determined by multiplex polymerase chain reaction-based assays using the specific primer sets. Results: A total of 29 staphylococcal strains (29/260, 11.1%) were isolated from samples of retail pork meat, 24 (83%) of which were CoNS. The four CoNS species identified were S. saprophyticus (n = 16, 55%), S. sciuri (n = 3, 10%), S. warneri (n = 3, 10%), and S. epidermidis (n = 2, 7%). Among the 29 isolates, four methicillin-resistant CoNS (MR-CoNS; three S. sciuri and one S. epidermidis) and one methicillin-resistant CoPS (MR-CoPS; one S. aureus) were identified. In addition, a relatively high level of tetracycline (TET) resistance (52%) was confirmed in CoNS, along with a predominant distribution of tet(K). The most prevalent SEs were sep (45%), and sen (28%), which were carried by 81% of S. saprophyticus. Conclusion: These findings suggest that CoNS, especially S. saprophyticus strains, in raw pork meat could be a potential risk factor for staphylococcal food poisoning (SFP), and therefore, requires further investigation to elucidate the role of SEls in SFP and virulence of the pathogen. Our results also suggest that CoNS from raw pork meat may act as a source for transmission of antimicrobial resistance genes such as staphylococcal cassette chromosome mec and tet(K).

Noonan syndrome and RASopathies: Clinical features, diagnosis and management

  • Lee, Beom Hee;Yoo, Han-Wook
    • Journal of Genetic Medicine
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    • v.16 no.1
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    • pp.1-9
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    • 2019
  • Noonan syndrome (NS) and NS-related disorders (cardio-facio-cutaneous syndrome, Costello syndrome, NS with multiple lentigines, or LEOPARD [lentigines, ECG conduction abnormalities, ocular hypertelorism, pulmonic stenosis, abnormal genitalia, retardation of growth and sensory neural deafness] syndrome) are collectively named as RASopathies. Clinical presentations are similar, featured with typical facial features, short stature, intellectual disability, ectodermal abnormalities, congenital heart diseases, chest & skeletal deformity and delayed puberty. During past decades, molecular etiologies of RASopathies have been growingly discovered. The functional perturbations of the RAS-mitogen-activated protein kinase pathway are resulted from the mutation of more than 20 genes (PTPN11, SOS1, RAF1, SHOC2, BRAF, KRAS, NRAS, HRAS, MEK1, MEK2, CBL, SOS2, RIT, RRAS, RASA2, SPRY1, LZTR1, MAP3K8, MYST4, A2ML1, RRAS2). The PTPN11 (40-50%), SOS1 (10-20%), RAF1 (3-17%), and RIT1 (5-9%) mutations are common in NS patients. In this review, the constellation of overlapping clinical features of RASopathies will be described based on genotype as well as their differential diagnostic points and management.

NOONAN SYNDROME : A CASE REPORT (누난 증후군 : 증례보고)

  • Lee, Soo-Eon;Choi, Sung-Chul;Kim, Kwang-Chul;Na, Sung-Sik;Park, Jae-Hong
    • The Journal of Korea Assosiation for Disability and Oral Health
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    • v.7 no.2
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    • pp.123-126
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    • 2011
  • Noonan syndrome (NS) is a developmental disorder characterized by dysmorphic facial features in association with short stature, mental retardation and congenital heart disease. NS may be sporadic or inherited as an autosomal dominant or recessive trait. The children with NS usually have ocular hypertelorism, downslanting palpebral fissures, low-set ears and a webbed neck, chest deformity. In addition, oral features include micrognathia, high arched palate, dental malocclusion, dental anomalies and rarely, cleft palate. The phenotype of NS bears similarities to that of Turner syndrome. However, NS occurs in both males and females with a normal sex chromosome 46, XX and 46, XY constitution. This case presents the intermittent treatment of an 8-year-old girl who was referred from a local clinic for the extraction of supernumerary teeth and treatment of dental caries. The focus of this case report is the oral aspects on NS and particularities of the dental treatment in subjects affected by this genetic disease.

Development of National Lightning Impulse Voltage Standard Measuring Technology (뇌충격전압 국가표준 측정기술 개발)

  • Kim, M.K.;Jeong, J.Y.;Kim, I.S.
    • Proceedings of the KIEE Conference
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    • 2003.07c
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    • pp.1744-1746
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    • 2003
  • This paper represents the development of national standard (NS) for lightning impulse (LI) voltage measuring system rated 400 kV. A traceability of the NS to the international standard could be achieved by the intercomparison test with Helsinki University. According to the IEC 60060-2, a measurement uncertainty was assessed. As a result of the tests, a measurement uncertainty and step response characteristics were satisfied with the requisite for NS.

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Intercomparison Test of 500 kV Standard Measuring System for SI Voltage (500 kV 표준급 개폐충격전압 측정시스템의 비교시험)

  • Kim, M.K.;Huh, D.H.;Kim, I.S.
    • Proceedings of the KIEE Conference
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    • 2007.07a
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    • pp.1492-1493
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    • 2007
  • This paper represents the development of national standard(NS) for switching impulse(SI) voltage measuring system rated 500 kV. A traceability of the NS to the international standard could be achieved by the intercomparison test with PTB(Physikalisch-Technische Bundesansalt). According to the IEC 60060-2, a measurement uncertainty was assessed. As a result of the tests, the measurement uncertainty and the characteristics of step response were satisfied with the requisite for NS.

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Numerical Analysis for Shotcrete Lining at SCL Tunnel in NS2 Transmission Cable Tunnel Project in Singapore (싱가포르 케이블터널 프로젝트 NS2현장 SCL 터널에서의 숏크리트 라이닝의 변형거동 특성)

  • Kwang, Han Fook;Kim, Young Geun
    • Tunnel and Underground Space
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    • v.27 no.4
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    • pp.185-194
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    • 2017
  • This technical paper is a study on the unique displacements of Shotcrete Lining at the mined tunnel during excavation period through deep consideration with real time data from monitoring instrumentations correlation with the numerical analysis to identify the rock stresses and the rock spring points at the working face of the Conventional tunnelling by the Drill and Blast, based on the geological face mapping results of the project NS2, Transmission cable tunnel project in Singapore. The created geometry of numerical model was prepared to the real mined tunnel construction site including, vertical shaft, construction adit, tunnel junction area, and 2 enlargement caverns. The convergence measurements by the monitoring instrumentation were performed during the tunnel excavation and shaft sinking construction stages to guarantee the safety of complicated underground structures.

Effects of Compost Leachate and Concentrated Slurry on the Growth and Yield of Pepper in a Substrate Hydroponic Culture (퇴비단 여과액비와 막분리 농축액비를 이용한 고형배지경 양액재배가 고추의 생육과 수량에 미치는 영향)

  • Ryoo, Jong-Won
    • Journal of Animal Environmental Science
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    • v.15 no.2
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    • pp.161-170
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    • 2009
  • This experiment was conducted to investigate the effects of compost leachate and concentrated pig slurry on growth of pepper in substrate hyrdoponic culture. In process of composting, compost leachate was was through a saturated compost heap. Pig slurry was filtered by ultra filtration and concentrated by reverse osmosis process. The pig slurry was mixed with chemical nutrient solution and byproduct based on nitrogen content. Peppers were grown in the seven different hydroponic solutions; compost leachate (CL), concentrated pig slurry (CS), compost leachate + byproduct (CL+BP), concentrated pig slurry + byproduct (CS+BP), compost leachate 50% + nutrient solution 50% (CL+NS), concentrated pig slurry 50% + nutrient solution 50% (CS+NS) and chemical nutrient solution for pepper. The chemical nutrient solution was the standard solution of National Horticulture Research Station for the growth of pepper. The concentration of nutrient solution was adjusted $1.6{\sim}2.0mS/cm$ in EC. The compost leachate and concentrated pig slurry were low in phosphorus (P), calcium (Ca), magnesium (Mg), but rich in potassium (K). Growth characteristics as affected by the different nutrient solution were significantly different. Growth and fruit characteristics treated with CL 50+NS 50%CS and 50+NS 50% were similar with NS 100% control plot. The dry weight of stem and leaf were 107.4, 104.2g in plot of NS 100% and CS 50%+NS 50%, respectively. The fruit of pepper showed lowest in the plot treated with 100% concentrated pig slurry, and the growth of pepper severely decreased after application of 100% CS treatment. The yield of pepper was not significantly different between the plots treated with mixture of CS50 + NS50% and 100% nutrient solution treatment. Fruit yield of the compost leachate concentrated pig slurry plot were 59, 14% compared to control, repectively. In conclusion, the mixture solution of 50% of pig slurry and 50% of nutrient solution could be used as a nutrition solution of pepper in hydroponic culture.

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Aero-Sim: An NS-2 Based simulator for Aeronautical Ad Hoc Networks

  • Luo, Qin;Wang, Junfeng;Wang, Xiaoqing;Wu, Ke
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.9 no.7
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    • pp.2548-2567
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    • 2015
  • Recently, there has been a clear trend towards the application of ad hoc networking technology in civil aviation communication systems, giving birth to a new research field, namely, aeronautical ad hoc networks (AANETs). An AANET is a special type of ad hoc wireless network with a significantly larger scale and distinct characteristics of its mobile nodes. Thus, there is an urgent need to develop a simulator to facilitate the research in these networks. In this paper, we present a network simulator, Aero-Sim, for AANETs. Aero-Sim, which is based on the freely distributed NS-2 simulator, enables detailed packet-level simulations of protocols at the MAC, link, network, transport, and application layers by composing simulations with existing modules and protocols in NS-2. Moreover, Aero-Sim supports three-dimensional network deployment. Through several case studies using realistic China domestic air traffic, we show that the proposed simulator can be used to simulate AANETs and can reproduce the real world with high fidelity.

Anti-Obesity Effects of Salted and Unsalted Doenjang Supplementation in C57BL/6J Mice Fed with High Fat Diet (식이유도 비만 Mice에서 된장 및 무염된장의 항비만 효과)

  • Bae, Cho-Rong;Kwon, Dae Young;Cha, Youn-Soo
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.42 no.7
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    • pp.1036-1042
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    • 2013
  • The aim of the present study is to elucidate the anti-obesity effects of Doenjang with and without salt in C57BL/6J mice. For the analysis, a total of forty mice were randomly divided into four groups: normal diet group (ND), high-fat diet group (HD), high-fat diet supplemented with 20% Doenjang group (DJ), high-fat diet supplemented with 20% unsalted Doenjang group (NS). During the study period, food intake and body weight were measured daily and weekly, respectively. The animals were sacrificed after 12 weeks. Body weight gain, epididymal fat pad weight and serum triglyceride levels of DJ group were found to be significantly lower than those of the HD and NS groups. Serum total-cholesterol levels of DJ and NS groups were significantly lower as compared to the HD group. There were significant decreases in plasma insulin and leptin levels in DJ group compared with the HD and NS groups. We did not observe any significant changes in the expression of hepatic lipogenic-related gene $PPAR{\gamma}$ among the HD, DJ and NS groups. However, ACC expression was found to be significantly decreased in DJ group. Lipolysis-related gene ($PPAR{\alpha}$ and CPT-1) expression was significantly higher in the DJ group as compared to HD and NS groups. In conclusion, the results of this study showed that Doenjang supplementation lowers body weight gain and improves obesity-related parameters.