• Title/Summary/Keyword: nrITS

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Molecular identification of medicinal herbs, Oldenlandia diffusa and Oldenlandia corymbosa based on nrDNA ITS region sequence

  • Sun, Yan-Lin;Wang, Dong;Yeom, Myung-Hun;Kim, Duck-Hee;Kim, Han-Gon;Hong, Soon-Kwan
    • Journal of Plant Biotechnology
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    • v.38 no.4
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    • pp.301-307
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    • 2011
  • The medicinal herb Oldenlandia diffusa is known as a folk medicine for the treatment of hepatitis, sore throat, appendicitis, malignant tumors and urethral infection in Southern China and Korea. Another species O. corymbosa, is also used for the therapy of the similar conditions, however, only O. diffusa is referred to the medicinal herb by Chinese Pharmacopoeia. Due to their similar morphology, O. diffusa and O. corymbosa are often misidentified. To easily identify O. diffusa from O. corymbosa, the phylogenetic utility of nuclear ribosomal DNA (nrDNA) internal transcribed spacers (ITS) were investigated among different O. diffusa and O. corymbosa populations in Korea. The nrDNA ITS sequence of O. diffusa contained 791 bp, with GenBank accession number of JF837601-JF837602. The nrDNA ITS sequence of O. corymbosa was 785-786 bp, with GenBank accession number of JF837603-JF837611. The results showed that there are some certain divergences in the ITS region sequence between both species, even among different populations of the same species. Particularly, O. corymbosa ST-4 population showed the highest dissimilarity of the ITS region sequence with other nine populations of O. corymbosa and two populations of O. diffusa. This consequence makes us further understand the molecular diversification between O. corymbosa and O. diffusa, and help to promote the correct use and safety.

Metal Complexes of Ambidentate Ligands (Ⅲ). Palladium (Ⅱ) Complexes of Isonitrosobenzoylacetone Imine Derivatives (Ambidentate 리간드의 금속착물 (제 3보). Isonitrosobenzoylacetone Imine 유도체와 팔라듐(Ⅱ)의 착물)

  • Tae Sub O;Man Ho Lee;Su Han Kim;Jung Hak Park;Hae Woon Lee
    • Journal of the Korean Chemical Society
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    • v.26 no.1
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    • pp.31-35
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    • 1982
  • Novel palladium(II) complexes of the type Pd(IBA-NH)(IBA-NH') and Pd(IBA-NR)2, where IBA-NH and IBA-NR (R: methyl, ethyl, n-propyl, n-butyl, benzyl) represent isonitrosobenzoylacetone imine and its N-alkyl derivatives respectively, have been prepared. The ir, nmr, and electronic spectra of the palladium(Ⅱ) complexes have been studied. It has been determined that the isonitroso group of IBA-NH coordinates to palladium through the oxygen to form 6-membered chelate ring and that of IBA-NH' coordinates to palladium through the nitrogen to form 5-membered ring in square-planar Pd(IBA-NH)(IBA-NH') and it also has been determined that the isonitroso groups of two IBA-NR coordinates to palladium through the nitrogen to form 5-membered rings in square-planar Pd$(IBA-NR)_2$. The coordination manner of the ligands is similar to that of isonitrosoacetylacetone imines obtained by Bose, et $al.^5$.

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A New Species of Hyphomycetes, Aspergillus coreanus sp.nov.,Isolated from Traditional Korean Nuruk

  • Yu, Tae-Shick;Yeo, Soo-Hwan;Kim, Hyun-Soo
    • Journal of Microbiology and Biotechnology
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    • v.14 no.1
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    • pp.182-187
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    • 2004
  • Strain NR $15-1^T$ isolated from traditional Korean Nuruk is described as a new species and named as Aspergillus coreanus NR $15-1^T$ sp. novo Strain NR $15-1^T$ grew rapidly to form yellow-green colonies whose surfaces were velvety on Czapek solution agar. Conidial heads were yellow to light and elliptical, whereas the conidiophore was colorless and typically long. In addition, vesicles were from flask-shaped to globose, and sterigmata are uniseriate. Conidia were spherical and deep yellow-green, and their surfaces were lightly roughened. The G+C content of strain NR $15-1^T$ was 51 mol% and strain NR $15-1^T$contained a dihydrogenated ubiquinone with Q9 (94.9%) as a major quinone. The nucleotide sequences of strain NR $15-1^T$ in the two Internal Transcribed Spacers (ITS 1 and 2) and 5.8S rDNA showed highest similarity when compared with that of A. tubingensis and A. phoenicis NRRL $365^T$. However, based on morphological and chemotaxonomic characteristics, this strain was different from A. tubingensis and A. phoenicis NRRL $365^T$. On the basis of the data presented, it is proposed that strain NR $15-1^T$ should be placed in the genus Aspergillus as a new species, Aspergillus coreanus sp. novo Therefore, the type strain of the new species is strain NR $15-1^T$ (=KCTC 18075P^T,=KCCM 80006^T$.

Analysis of Requirements for Night Vision Imaging System (야시조명계통 요구도 분석)

  • Kwon, Jong-Kwang;Lee, Dae-Yearl;Kim, Whan-Woo
    • Journal of the Korea Institute of Military Science and Technology
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    • v.10 no.3
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    • pp.51-61
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    • 2007
  • This paper concerns about the requirement analysis for night vision imaging system(NVIS), whose purpose is to intensify the available nighttime near infrared(IR) radiation sufficiently to be caught by the human eyes on a miniature green phosphor screen. The requirements for NVIS are NVIS radiance(NR), chromaticity, daylight legibility/readability, etc. The NR is a quantitative measure of night vision goggle (NVG) compatibility of a light source as viewed through goggles. The chromaticity is the quality of a color as determined by its purity and dominant wavelength. The daylight legibility/readability is the degree at which words are readable based on appearance and a measure of an instrument's ability to display incremental changes in its output value. In this paper, the requirements of NR, chromaticity, and daylight legibility/readability for Type I and Class B/C NVIS are analyzed. Also the rationale is shown with respect to those requirements.

Genetic Variation and Phylogenetic Relationship of Korean Ginseng based on cpDNA trnL-F, nrDNA ITS and ETS Sequences (엽록체 DNA trnL-F 및 핵리보조옴 DNA ITS, ETS 염기서열에 의한 고려인삼의 유전적 변이와 계통학적 유연관계)

  • Bang Chan Kuk;Kim Ju Hwan;Baek Myeong Hyun;Kim Chang Sik;Um Dong Myeong;Kim Dong Hee
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.18 no.6
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    • pp.1699-1709
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    • 2004
  • Genetic variations and relationship based on the sequences of cpDNA trnL-F gene, nrDNA ITS and ETS region among the twenty four taxa including Panax ginseng C.A. Meyer and its related species were investigated. And taxonomic status and molecular phylogenetic relationship between P. ginseng and related groups were discussed. Molecular systematic data from cpDNA and nrDNA sequences were very useful to elucidate the genetic variations and relationships among the treated taxa. It was found that P. ginseng is the independent unique species with distinct genetic limitation from the related species such as P. quinquefolius, P. japonicum, P. notoginseng and P. pseudoginseng. P. ginseng including cultivated types as well as wild ones formed monophyletic group with high genetic similarities. P. quinquefolius and P. japonicum were the most related sister groups of P. ginseng based on the molecular phylogenetic results in this study.

Preparation of NR/MG Latex Blend Films and its Mechanical Properties (NR/MG Latex 블랜드필름의 제조 및 그의 기계적특성)

  • Kim, K.S.;Park, J.H.;Eum, J.S.;Kim, S.J.
    • Elastomers and Composites
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    • v.29 no.1
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    • pp.9-17
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    • 1994
  • Methylmetharylate grafted latex(MGL) was prepared by emulsion graft copolymerization of methyl methacrylate onto natural rubber latex(NRL) by using t-butyl hydroperoxide and tetraethylene pentamine in an aqueous medium. Blending of MGL and NRL with different mixing ratio carried out and viscosity and particle size distribution of blend latex were determined. It was found that the optimum condition of mature time, vulcanizing temperature and time for preparation of blend latex films were investigated. latex films prepared by dipping process were meaured. As the reuslt, blend latex(NR-d-MG) films obtained from two-dipping system were more excellent than NR and MG film obtained from one-dipping system.

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Removal of haloacetonitrile by adsorption on thiol-functionalized mesoporous composites based on natural rubber and hexagonal mesoporous silica

  • Krueyai, Yaowalak;Punyapalakul, Patiparn;Wongrueng, Aunnop
    • Environmental Engineering Research
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    • v.20 no.4
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    • pp.342-346
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    • 2015
  • Haloacetonitriles (HANs) are nitrogenous disinfection by-products (DBPs) that have been reported to have a higher toxicity than the other groups of DBPs. The adsorption process is mostly used to remove HANs in aqueous solutions. Functionalized composite materials tend to be effective adsorbents due to their hydrophobicity and specific adsorptive mechanism. In this study, the removal of dichloroacetonitrile (DCAN) from tap water by adsorption on thiol-functionalized mesoporous composites made from natural rubber (NR) and hexagonal mesoporous silica (HMS-SH) was investigated. Fourier-transform infrared spectroscopy (FTIR) results revealed that the thiol group of NR/HMS was covered with NR molecules. X-ray diffraction (XRD) analysis indicated an expansion of the hexagonal unit cell. Adsorption kinetic and isotherm models were used to determine the adsorption mechanisms and the experiments revealed that NR/HMS-SH had a higher DCAN adsorption capacity than powered activated carbon (PAC). NR/HMS-SH adsorption reached equilibrium after 12 hours and its adsorption kinetics fit well with a pseudo-second-order model. A linear model was found to fit well with the DCAN adsorption isotherm at a low concentration level.

Rubber bound phenolic antioxidant and its application in thermoplastic elastomer

  • Klinpituksa, Pairote;Kiarttisarekul, Anyarat;Kaesaman, Azizon
    • Advances in materials Research
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    • v.3 no.3
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    • pp.175-183
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    • 2014
  • Natural rubber bound phenolic antioxidant, 2,6-di-tert-butyl-4-vinylphenol (2,6-DBVP), was prepared from natural rubber and 2,6-DBVP in both solution and melt state. The 2,6-DBVP had been synthesized from 3,5-di-tert-butyl-4-hydroxybenzaldehyde and methyltriphenylphosphonium iodide ($MePPh_3I$) by Wittig reaction ($0^{\circ}C$ for 2 hrs, $N_2$ atmosphere). The conditions for preparation of natural rubber bound 2,6-DBVP (NR-DBVP) were optimized for both solution state (1 phr BPO and 8 phr 2,6-DBVP at $70^{\circ}C$ for 2 hrs) and for melt state (1 phr BPO and 8 phr 2,6-DBVP at $70^{\circ}C$ for 10 mins, with rotor speed of 60 rpm). A thermoplastic vulcanizate was obtained using a compatibilizer, polypropylene modified with phenolic resin (PhHRJ-PP), in a closed mixer ($180^{\circ}C$ for 3 mins, rotor speed 60 rpm). The antioxidant properties of vulcanized NR-DBVP, using phenolic as the vulcanization system, were similar to NR with the conventional antioxidant BHT. In addition, the antioxidant, water leaching property of the thermoplastic vulcanizate of NR-DBVP/PP were good in comparison to a NR blend with BHT; the morphologies of these thermoplastic vulcanizates were similar.

Metal Complexes of Ambidentate Ligands (I). Nickel(II) Complexes of Isonitrosobenzoylacetone Imine Derivatives (Ambidentate Ligand의 금속 착물 (제1보). Isonitrosobenzoylacetone Imine 유도체와 니켈(Ⅱ)의 착물)

  • Man Ho Lee;Dae Sub Oh;Kwang Woo Lee
    • Journal of the Korean Chemical Society
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    • v.22 no.1
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    • pp.19-24
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    • 1978
  • Novel nickel(II) complexes of the type Ni(IBA-NH)(IBA-NR), where IBA-NH and IBA-NR (R=H, methyl, ethyl, n-propyl, iso-propyl, n-butyl or benzyl) represent isonitrosobenzoylacetone imine and its N-alkyl derivative respectively, have been prepared. The ir, nmr, and electronic spectra and magnetic moment of the nickel(II) complexes have been studied. It has been determined that the isonitroso group of IBA-NH coordinates to nickel through the oxygen to form 6-membered chelate ring and that of IBA-NR coordinates to nickel through the nitrogen to form 5-membered ring in square-planar Ni(IBA-NH) (IBA-NR). The coordination manner of the ligands is similar to that of isonitrosoacetylacetone imines obtained by Bose, et al.

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