• Title/Summary/Keyword: nrITS

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The Genus Martensia Hering (Delesseriaceae, Rhodophyta) with M. albida sp. nov. and M. flammifolia sp. nov. on Jeju Island, Korea

  • Lee, Yong-Pil
    • ALGAE
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    • v.21 no.1
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    • pp.15-48
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    • 2006
  • The genus Martensia (Delesseriaceae, Rhodophyta) is characterized by thalli composed of one to several blades that consist of proximal membranous sections and distal latticework. Nerves or veins are absent in the membranous sections. The life cycle of Martensia is accomplished by isomorphic alternation of generations. The gametophytes of Martensia are dioecious, and the male and female gametangial plants are morphologically similar. The type species of Martensia is M. elegans Hering. In this study, nine species were confirmed to occur in the subtidal regions of Jeju Island, Korea: M. albida sp. nov., M. australis Harvey, M. bibarii Y. Lee, M. elegans Hering, M. flammifolia sp. nov, M. fragilis Harvey, M. jejuensis Y. Lee, M. palmata Y. Lee, and M. projecta Y. Lee. Three of these, M. australis, M. fragilis, and M. elegans, are new records in the flora of Korea. The results of molecular analyses of the internal transcribed spacer (ITS) 1 region in the nrDNA showed that M. elegans is identical to M. australis, and M. fragilis coincides with M. bibarii. It may be a less effective tool for the species discrimination in Martensia.

Application of Bacterial Foraging Algorithm and Genetic Algorithm for Selective Voltage Harmonic Elimination in PWM Inverter

  • Maheswaran, D.;Rajasekar, N.;Priya, K.;Ashok kumar, L.
    • Journal of Electrical Engineering and Technology
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    • v.10 no.3
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    • pp.944-951
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    • 2015
  • Pulse Width Modulation (PWM) techniques are increasingly employed for PWM inverter fed induction motor drive. Among various popular PWM methods used, Selective Harmonic Elimination PWM (SHEPWM) has been widely accepted for its better harmonic elimination capability. In addition, using SHEPWM, it is also possible to maintain better voltage regulation. Hence, in this paper, an attempt has been made to apply Bacterial Foraging Algorithm (BFA) for solving selective harmonic elimination problem. The problem of voltage harmonic elimination together with output voltage regulation is drafted as an optimization task and the solution is sought through proposed method. For performance comparison of BFA, the results obtained are compared with other techniques such as derivative based Newton-Raphson method, and Genetic Algorithm. From the comparison, it can be observed that BFA based approach yields better results. Further, it provides superior convergence, reduced computational burden, and guaranteed global optima. The simulation results are validated through experimental findings.

Arthonia dokdoensis and Rufoplaca toktoana - Two New Taxa from Dokdo Islands (South Korea)

  • Kondratyuk, Sergij;Lokos, Laszlo;Halda, Josef;Lee, Beeyoung Gun;Jang, Seol-Hwa;Woo, Jeong-Jae;Park, Jung Shin;Oh, Soon-Ok;Han, Sang-Kuk;Hur, Jae-Seoun
    • Mycobiology
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    • v.47 no.4
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    • pp.355-367
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    • 2019
  • Arthonia dokdoensis sp. nov., a lichenicolous fungus from the subcosmopolitan Arthonia molendoi complex growing on crustose thalli of species of the genus Orientophila (subfamily Xanthorioideae, Teloschistaceae), as well as the lichen species Rufoplaca toktoana sp. nov. (subfamily Caloplacoideae, Teloschistaceae) similar to Rufoplaca kaernefeltiana, both from Dokdo Islands, Republic of Korea, are described, illustrated, and compared with closely related taxa. In the phylogenetic tree of the Arthoniaceae based on 12S mtSSU and RPB2 gene sequences, the phylogenetic position of the A. dokdoensis and the relationship with the A. molendoi group are illustrated, while the position of the newly described R. toktoana is confirmed by phylogenetic tree based on ITS nrDNA data.

THE CYTOTOXICITY ON L929 CELLS AND ANTIMICROBIAL EFFECT ON SEVERAL STREPTOCOCCI OF CALCIUM HYDROXIDE (수산화칼슘의 L929 세포독성 및 연쇄구균에 대한 항균효과에 관한 연구)

  • Yu, Young-Dae;Im, Mi-Kyung
    • Restorative Dentistry and Endodontics
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    • v.20 no.2
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    • pp.538-548
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    • 1995
  • Calcium hydroxide has been used not only as pulp capping and pulpotomy agents in the operative dentistry, but dressing and temporary filling materials in root canal treatment. Calcium hydroxide was known to stimulate odontoblast to produce new reparative dentin and to eliminate microorganims effectively in the infected root canals. The purpose of this study was to evaluate the effect of calcium hydroxide solution on cultured L929 cells and its antibacterial effect on several streptococci. Calcium hydroxide solution (0.121g/100ml) was added to L929 cells and cell viability was measured using 3-(4,5-dimethylthiazol-2-yl) -2,5-dimethyltetrazolium bromide (MTT) and neutral red (NR) dye. Calcium hydroxide solution (20, 40, 60, 80, 100 and $150{\mu}l$) was added to L929 cells in 96-well microplates for 1, 4 and 24 hours respectively. Cell viability was gradually decreased when the volume and exposure time of calcium hydroxide solution were increased. When $150{\mu}l$ of calcium hydroxide was applied to L929 cells for 24 hours, there was more than fifty percent reduction of cell viability. Calcium hydroxide solution (20g/100ml) showed antibacterial effect against S. uberis, S. intermedius and S. mitis after thirty-second exposure. But 0.121g/100ml concentration of cacium hydroxide solution exhibited no antibacterial effect on six streptococci after one-hour exposure.

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Taxonomic Review of the Genus Echinochloa in Korea (I): Inferred from Sequences of cpDNA and nrDNA

  • Lee, Jeongran;Kim, Chang-Seok;Lee, In-Yong
    • Weed & Turfgrass Science
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    • v.3 no.3
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    • pp.183-189
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    • 2014
  • The genus Echinochloa (L.) P. Beauv. comprised of approximately 30-40 species in the tropical and warm temperate regions of the world, including numerous interspecific and intraspecific types which make the genus difficult to identify. As an attempt to identify the species within the genus easier, the taxonomy of the genus Echinochloa, Poaceae in Korea was reviewed on the basis of sequencing data derived from nuclear ribosomal DNA internal transcribed spacer (ITS) and external transcribe spacer and chloroplast DNA trnL intron, trnL-F intergenic spacer and matK regions using a total of 46 accessions representing all the species in Korea. The results of maximum parsimony found separate lineage comprised of E. colona and E. frumentaceae which are not Korean species, but no resolution within Korean Echinochloa species, supporting the suggestion of Yamaguchi group that E. crus-galli, E. oryzoides, and E. esculenta should be considered to belong to the same species. However, the relationship between these three species and the other species, i.e. E. oryzicola should be better understood with more detail studies.

A Study of Improving Transmissibility for Grommets in Air conditioner Compressor (에어컨 압축기 진동전달률 개선을 위한 그로메트 연구)

  • Park, Hong-Ul;Lee, Jai-Kwon;Mo, Jin-Yong;Park, Deug-Yong;Han, Hyung-Suk
    • Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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    • 2004.11a
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    • pp.651-655
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    • 2004
  • This paper studies the designing method and application for grommets, rubber material used to absorb vibration on the air conditioner compressor. The existing grommet with hardness 40 degrees, EPDM, has the high transmissibility on the compressor that causes additional structure born sound. The problem for EPDM is transformation over long time usage possibly due to its design in which stress is concentrated on a certain position. In order to resolve it, silicon material was previously used with the same design in some models. The vibration performance did improve, but the cost became high. Below are the major developments regarding improvements in compressor rubber material, vibration performance and durability through design change, and new grommet to attain cost reduction. 1 The optimum grommet design for stress even distribution through FEM methods. 2. Comparison for grommet material and design for improved transmissibility. 3. Assess for grommets durability and product applications.

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Taxonomic Revision of the Genus Lactifluus (Russulales, Basidiomycota) of South Korea

  • Lee, Hyun;Wissitrassameewong, Komsit;Park, Myung Soo;Fong, Jonathan J.;Verbeken, Annemieke;Kim, Changmu;Lim, Young Woon
    • Mycobiology
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    • v.49 no.4
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    • pp.308-345
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    • 2021
  • Lactifluus (Pers.) Roussel is an ectomycorrhizal genus that was recently recognized to be distinct from the genus Lactarius. To date, 226 Lactifluus species have been reported worldwide. Misidentification of Lactifluus species is common because of intraspecific morphological variation, cryptic diversity, and the limited number of taxonomic keys available. Molecular data are indispensable for species delimitation; a multilocus phylogenetic analysis showed that most Asian Lactifluus species are not conspecific with morphologically similar species present on other continents. In particular, Korea has misused European and North American Lactifluus names. In this study, we evaluated the taxonomy of Lactifluus in Korea using both morphological and multilocus molecular (ITS, nrLSU, rpb1, and rpb2) data. We examined 199 Lactifluus specimens collected between 1980 and 2016, and a total of 24 species across the four Lactifluus subgenera were identified. All Korean species are distinct and clearly separated from European and North American species. Five taxa corresponded to previously described species from Asia and the remaining 19 taxa are confirmed as new species. Herein, we provide keys to the Korean Lactifluus species within their subgenera, molecular phylogenies, a summary of diversity, and detailed description of the new species.

Taxonomic Review on Hydrangea macrophylla (Thunb.) Ser. and H. serrata f. acuminata (Siebold & Zucc.) E.H.Wilson (산수국과 수국의 분류학적 재검토)

  • Kang, Shin-Ho;Chung, Kyong-Sook
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2019.10a
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    • pp.22-22
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    • 2019
  • 수국속(Hydrangea L.)은 APG IV분류체계에 따르면 국화군(Asterids), 층층나무목(Cornales), 수국과(Hydrangeaceae)에 속하는 식물로서 동아시아, 북아메리카 동부 지역을 중심으로 분포하며 전 세계에 23여종이 알려져 있다. 한국산 수국속은 Nakai가 H. hortensia var. acuminata의 분포를 기록함으로써 시작되었다. 이후 Nakai는 산수국을 H. acuminata로 재처리, 다시 H. serrata var. acuminata로 변경하였다. 한편 산수국은 분류학적 위치에 대한 이견이 있다. Wilson은 수국과 산수국을 가까운 유연관계를 가지는 독립된 분류군으로 분류하였지만, Makino는 산수국을 수국의 아종인 H. marcrophylla subsp. serrta로 기재하는 등 산수국과 수국의 동정과 계급설정에 혼란이 있는 가운데, 최근 천연물 hydrangenol을 활용한 건강기능식품 개발에서 사용가능 재료의 한계를 설정하기 위한 산수국과 수국에 대한 종 설정 연구도 요구되고 있다. 따라서 본 연구에서는 nrDNA ITS, trnL-F, trnC-ycf6의 3개 구간의 DNA sequence와 NCBI (National Center for Biotechnology Information)를 참고한 분자생물학적 분석결과와 외부형태학적 재검토 결과를 통하여 H. marcrophylla를 종(Species)으로서의 분류학적 위치 지지하며 또한 수국과 산수국을 가까운 유연관계를 가지는 독립된 분류군으로 분류한 의견을 지지하는 결과를 도출하였다. 향후 hydrangenol 활용의 기초정보로 활용되기를 기대한다.

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Occurrence and Characterization of Leaf Spot Caused by Septoria melissae on Lemon Balm in Korea

  • Yang, Seon-Ah;Choi, In-Young;Ju, Ho-Jong;Lee, Kui-Jae;Galea, Victor;Shin, Hyeon-Dong
    • Mycobiology
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    • v.48 no.6
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    • pp.495-500
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    • 2020
  • Leaf spot on lemon balm is frequently observed in Korea, causing considerable damage to crops. In 2014 and 2015, the occurrence of leaf spot was observed in several production greenhouses at Suwon, Gongju, and Namwon in Korea. Symptoms on lower leaves initially developed as small, distinct, discolored lesions, which enlarged progressively turning into dark brown, angular spots surrounded by purplish-brown margins. Based on the morphological characteristics and sequence analysis of actin (ACT), translation elongation factor 1-alpha (EF-1α), internal transcribed spacer (ITS), 28S nrDNA (LSU), and RNA polymerase II second largest subunit (RPB2), the fungus associated with the lemon balm leaf spot was determined as Septoria melissae. To the best of our knowledge, this is the first report of lemon balm leaf spot caused by S. melissae in Asia as well as in Korea.

Unveiling the Diversity of Hydnum in the Republic of Korea with One New Species, Hydnum paucispinum

  • Ji Seon Kim;Wonjun Lee;Changmu Kim;Hanna Park;Chang Sun Kim;Young Woon Lim
    • Mycobiology
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    • v.51 no.5
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    • pp.300-312
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    • 2023
  • Hydnum is a genus of ectomycorrhizal fungi belonging to the Hydnaceae family. It is widely distributed across different regions of the world, including North America, Europe, and Asia; however, some of them showed disjunct distributions. In recent years, with the integration of molecular techniques, the taxonomy and classification of Hydnum have undergone several revisions and advancements. However, these changes have not yet been applied in the Republic of Korea. In this study, we conducted an integrated analysis combining the morphological and molecular analyses of 30 specimens collected over a period of approximately 10 years in the Republic of Korea. For molecular analysis, the sequence data of the internal transcribed spacer (ITS) region, the large subunit of nuclear ribosomal RNA gene (nrLSU), and a portion of translation elongation factor 1-a (TEF1) were employed as molecular markers. Through this study, we identified eight species that had previously not been reported to occur in the Republic of Korea, including one new species, Hydnum paucispinum. A taxonomic key and detailed descriptions of the eight Hydnum species are provided in this study.