• Title/Summary/Keyword: nrITS

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Taxonomic Study on the Lichen Genus Cetrelia (Lecanorales, Ascomycota) in South Korea

  • Luo, Heng;Wei, Xin-Li;Han, Keon-Seon;Koh, Young-Jin;Hur, Jae-Seoun
    • Mycobiology
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    • v.35 no.3
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    • pp.117-123
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    • 2007
  • Seventy-two lichen specimens of Cetrelia collected in South Korea since 2003 were examined by both phenotypic and phylogenetic analyses. The phenotypic analysis was based on morphological and chemical characters, and the phylogenetic analysis was based on nrDNA ITS sequences. The result suggested that the presence and absence of isidia, soredia, lobules and medullar reaction C+ or C- are the important characters in the taxonomy of this genus. Four species of Cetrelia, C. chicitae, C. braunsiana, C. japonica, and C. pseudolivetorum have been identified in this study. Description of each species is presented with morphological and chemical characters. A key to the Cetrelia species is also presented.

Phylogenetic Relationships of Soranthera ulvoidea (Chordariaceae, Phaeophyceae) on the Basis of Morphology and Molecular Data

  • Cho, Ga-Youn;Kim, Myung-Sook;Boo, Sung-Min
    • ALGAE
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    • v.20 no.2
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    • pp.91-97
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    • 2005
  • The brown algal family Chordariaceae sensu lato is a focus of taxonomy because recent studies suggest a broad concept of the family, including genera formerly classified in the Dictyosiphonales. Using morphology, plastid rbcL and nrDNA ITS sequences, we evaluated relationships of the monotyic genus Soranthera (S. ulvoidea), which has been classified in the Punctariaceae. The species occurs in Bering Sea and Aleutian Islands, Alaska to Baja California. Thalli are globose to lobed, hollow, 3-5 cm in diameter, and covered with evenly distributed sori. However, two forms within the species are recognized: f. ulvoidea for globose forms and f. difformis for lobed forms. Plastid rbcL and nuclear ITS region sequences were newly determined in samples of S. ulvoidea from the Pacific coast of the North America. We found little variations in the ITS sequences among samples of S. ulvoidea from five different locations and in the rbcL region from two different locations. These results do not support previous classification of f. ulvoidea and f. difformis within the species. All analyses of our rbcL sequence dataset show that Soranthera was placed in the Chordariaceae s.l., but more related to Botrytella than Punctaria and Asperococcus.

Comparison of the Postoperative Pain Control Effects of a Buprenorphine Transdermal Patch on Total Knee Arthroplasty Surgery Patients according to Its Applied Sites: Retrospective Case-Control Study (슬관절 인공관절 전치환술 환자에서 부프레노르핀 경피 패치의 적용부위에 따른 수술 후 통증 조절 효과 비교: 후향적 환자-대조군 연구)

  • Kim, Ok-Gul;Lee, Sang-Wook;Kim, Hyun-Min
    • Journal of the Korean Orthopaedic Association
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    • v.55 no.6
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    • pp.527-533
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    • 2020
  • Purpose: This study compared the effects of a buprenorphine transdermal patch (BTDP) on the chest and knee for pain control after total knee arthroplasty (TKA). Materials and Methods: A retrospective case-control study was conducted from August 2018 to August 2019 on 231 patients who underwent TKA. Two hundred cases were selected considering age, sex, and body mass index. Before and after applying the BTDP, the Numeric Rating Scale (NRS), adverse effects and compliance were measured. All measurements in the chest application group (group A=100) and knee application group (group B=100) were compared. Results: NRS was similar in rest between the groups treated with BTDP, but at two days and three days afternoon, five, six, and seven days postoperatively in group B, the NRS was significantly lower than that of group A. The adverse effects of the central nervous system and gastrointestinal system after applying BTDP were significantly lower in group B than in group A. No significant differences in adverse effects of the cardiovascular system and skin were observed between the two groups. Regarding the maintenance of BTDP, group B was significantly higher than group A. Conclusion: The direct application of BTDP after TKA to painful knee joints showed excellent results in early postoperative pain control and can be a useful method for increasing patient compliance by reducing the frequency of adverse effects.

NgR1 Expressed in P19 Embryonal Carcinoma Cells Differentiated by Retinoic Acid Can Activate STAT3

  • Lee, Su In;Yun, Jieun;Baek, Ji-Young;Jeong, Yun-Ji;Kim, Jin-Ah;Kang, Jong Soon;Park, Sun Hong;Kim, Sang Kyum;Park, Song-Kyu
    • The Korean Journal of Physiology and Pharmacology
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    • v.19 no.2
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    • pp.105-109
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    • 2015
  • NgR1, a Nogo receptor, is involved in inhibition of neurite outgrowth and axonal regeneration and regulation of synaptic plasticity. P19 embryonal carcinoma cells were induced to differentiate into neuron-like cells using all trans-retinoic acid and the presence and/or function of cellular molecules, such as NgR1, NMDA receptors and STAT3, were examined. Neuronally differentiated P19 cells expressed the mRNA and protein of NgR1, which could stimulate the phosphorylation of STAT3 when activated by Nogo-P4 peptide, an active segment of Nogo-66. During the whole period of differentiation, mRNAs of all of the NMDA receptor subtypes tested (NR1, NR2A-2D) were consistently expressed, which meant that neuronally differentiated P19 cells maintained some characteristics of neurons, especially central nervous system neurons. Our results suggests that neuronally differentiated P19 cells expressing NgR1 may be an efficient and convenient in vitro model for studying the molecular mechanism of cellular events that involve NgR1 and its binding partners, and for screening compounds that activate or inhibit NgR1.

EFFICIENCY OF PROTEIN UTILIZATION OF FORMALDEHYDE TREATED RAPESEED MEAL BY SHEEP AND ITS INFLUENCE ON CATTLE'S PERFORMANCE

  • Liu, J.X.;Wu, Y.M.;Xu, N.Y.;Wu, Z.W.
    • Asian-Australasian Journal of Animal Sciences
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    • v.6 no.4
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    • pp.601-605
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    • 1993
  • This study was purposed to investigate the efficiency of protein utilization of rapeseed meal (RSM) and formaldehyde-treated RSM (TRSM) by sheep, and their influence on performance of growing heifers. Experiment 1 was conducted according to a double $3{\times}3$ Latin square design involving sex yearling Hu Sheep and three experimental diets. All diets contained 600 g ammoniated rice straw and 200 g concentrate mixture. Three dietary treatments were: (1) 100 g RSM + 40 g soybean meal (URUS), (2) 100 g TRSM + 40 g soybean meal (TRUS) and (3) 100 g TRSM + 40 g treated soybean meal (TRTS). Apparent nitrogen digestibility was significantly higher for diet TRUS than that for URUS or TRTS (p<0.05), but with no significant difference between URUS and TRTS (p>0.05). Proportion of nitrogen retention (NR) to the digestible nitrogen intake for diet TRUS and TRTS was 25.57 (p<0.05) and 23.44% (p<0.05) higher than that for URUS respectively. As a result, proportion of NR to nitrogen intake for diet TRUS and TRTS was 34.74 (p<0.05) and 23.78% (p<0.05) higher than that for URUS respectively. Experiment 2 was conducted with 59 Holstein heifers. They were 12-20 months of age at the start of the trial. The experiment was a $2{\times}2$ factorial trial in which the heifers were given the ammoniated rice straw ad libitum and 1.5 kg hay, and supplemented with either RSM or TRSM at a daily allowance of 1.2 or 1.8 kg per day. The live weight gains for heifers receiving 1.2 and 1.8 kg/d of RSM or TRSM were 0.491 and 0.556 or 0.564 and 0.665 kg/d, respectively. The results suggest that formaldehyde treatment can effectively improve the efficiency of protein utilization of rapessed meal and cattle's performance.

Antihypertensive and Cardiovascular Effects of the New Calcium Antagonist YH334

  • Lee, Jong-Wook;Han, Byung-Hee;Lee, Jeong-Won;Seok, Ji-Hee;Kim, Su-Chang;Hong, You-Hwa;HongSuh, Jung-Jin;Hong, Soon-Uk
    • Archives of Pharmacal Research
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    • v.14 no.3
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    • pp.242-248
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    • 1991
  • Antihypertensive effect of YH 334 was examined in various experimental hypertension rat models and the systemic and regional hymohynamic profiles of the compound were investigated in conscious spontaneously hypertensive rats (SHR). The antiypertensive potensive potency of YH 334 is found to be more than 10 times stronger than that of nitrendipine in the all hypertensive models. The effective doses to lower the initial blood pressure by 20% $(ED_{20})$ of YH334 were 1.4 mg/kg in normotensive rats (NR), 0.7 mglkg in SHR. 0.1 mg/kg in DOCA salt hypertensive rats (DHR) and 0.4 mg/kg in renal hypertensive rats (RHR), and the $ED_{20}$ values of nitrendipine were 15.8 mg/kg in NR, 7.1 mg/kg in SHR, 1.7 mg/kg in DHR and 4.8 mg/kg in RHR. The primary hemodynamic effect hemodynamic profile is similar to that of nitrendipine. Both compounds seem to produce potent antihypertensive effects by lowering peripheral resistance in the skeletal muscles. In the organ bath study using isolated rabbit aorta, YH 334 was found to be a potent voltage dependent calcium channel blocker without significant inhibitory effect on the receptor operated calcium channels like the most of other dihydropyridine type calcium antagonists. Furthermore, YH334 showed acute diuretic and natriuretic effects in conscious SHR, which may render the unnecessary restriction of sodium in the diet of those patients on long term hypertension therapy. This effect would provide an additional benefit to its potent antihypertensive activity.

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Design of cellular, satellite, and integrated systems for 5G and beyond

  • Kim, Junhyeong;Casati, Guido;Cassiau, Nicolas;Pietrabissa, Antonio;Giuseppi, Alessandro;Yan, Dong;Strinati, Emilio Calvanese;Thary, Marjorie;He, Danping;Guan, Ke;Chung, Heesang;Kim, Ilgyu
    • ETRI Journal
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    • v.42 no.5
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    • pp.669-685
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    • 2020
  • 5G AgiLe and fLexible integration of SaTellite And cellulaR (5G-ALLSTAR) is a Korea-Europe (KR-EU) collaborative project for developing multi-connectivity (MC) technologies that integrate cellular and satellite networks to provide seamless, reliable, and ubiquitous broadband communication services and improve service continuity for 5G and beyond. The main scope of this project entails the prototype development of a millimeter-wave 5G New Radio (NR)-based cellular system, an investigation of the feasibility of an NR-based satellite system and its integration with cellular systems, and a study of spectrum sharing and interference management techniques for MC. This article reviews recent research activities and presents preliminary results and a plan for the proof of concept (PoC) of three representative use cases (UCs) and one joint KR-EU UC. The feasibility of each UC and superiority of the developed technologies will be validated with key performance indicators using corresponding PoC platforms. The final achievements of the project are expected to eventually contribute to the technical evolution of 5G, which will pave the road for next-generation communications.

Determination of Strain Energy Function of Rubber Materials Considering Stress Softening Behavior (응력연화거동을 고려한 고무 재료의 변형률 에너지 함수 결정)

  • Kim, W.S.;Hong, S.I.
    • Elastomers and Composites
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    • v.42 no.3
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    • pp.168-176
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    • 2007
  • When the rubber vulcanizates reinforced with carbon black or silica are subjected to cyclic loading from its virgin state, the stress required on reloading is less than that on the initial loading. This stress softening phenomenon is referred to as the Mullins effect. The strain energy function of rubber vulcanizates was investigated using theory of pseudo-elasticity incorporated damage parameter that Ogden and Roxburgh have proposed to describe the damage-induced stress softening effect in rubber-like solids. The quasi-static cyclic loading test was performed using the NR-SBR vulcanizates reinforced with carbon black, and then the effect of a damage parameter to stress-strain curve in reloading and subsequent reloading paths was studied. The strain energy function of the rubber vulcanizates with a different filler content was also evaluated.

Phylogenetic Relationship between Two Taxa of Opuntia in Jeju Island (제주도 부채(손바닥)선인장속(Opuntia) 두 분류군의 계통발생학적 유연관계)

  • Yang, Young-Soo;Oh, Hong-Shik
    • Korean Journal of Plant Resources
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    • v.34 no.5
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    • pp.451-461
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    • 2021
  • In Jeju Island, there are two different types of Opuntia cactus in cladodae, fruit, flower, flowering period and spine. There is no comparative study of these two taxa yet, so it is often seen as the same species. The purpose of this study was to investigate the phylogenetic relationship between the Taxa of the Opuntia monacantha Haw. and Opuntia stricta Haw. in Jeju Island. The results indicate that the two taxa were completely different from each other on the analysis of nrITS, matK and atpB-rbcL gene as well as the morphological comparison. Also, O. monacantha among the two taxa is included in the Elatae series, so South America is the origin, and O. stricta is included in the Scheerianae series, which confirms that North America is the origin. The name of the O. monacantha in Jeju Island was named 'Jeju-Baick-nyun-cho' considering geographical location conditions and symbolic characteristics.

Phylogenetic Relationships in Korean Elaeagnus L. Based on nrDNA ITS Sequences

  • Son, OGyeong;Yoon, Chang Young;Park, SeonJoo
    • Korean Journal of Plant Resources
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    • v.27 no.6
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    • pp.671-679
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    • 2014
  • Molecular phylogenetic analyses of Korean Elaeagnus L. were conducted using seven species, one variety, one forma and four outgroups to evaluate their relationships and phylogeny. The sequences of internal transcribed spacer regions in nuclear ribosomal DNA were employed to construct phylogenetic relationships using maximum parsimony (MP) and Bayesian analysis. Molecular phylogenetic analysis revealed that Korean Elaeagnus was a polyphyly. E. umbellata var. coreana formed a subclade with E. umbellata. Additionally, the genetic difference between E. submacrophylla and E. macrophylla was very low. Moreover, E. submacrophylla formed a branch from E. macrophylla, indicating that E. submacrophylla can be regarded as a variety. However, several populations of this species were not clustered as a single clade; therefore, further study should be conducted using other molecular markers. Although E. glabra f. oxyphylla was distinct in morphological characters of leaf shape with E. glabra. But E. glabra f. oxyphylla was formed one clade by molecular phylogenetic with E. glabra. Additionally, this study clearly demonstrated that E. pungens occurs in Korea, although it was previously reported near South Korea in Japan and China. According to the results of ITS regions analyses, it showed a resolution and to verify the relationship between interspecies of Korean Elaeagnus.