• Korean Journal of Plant Resources
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• v.6 no.2
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• pp.135-140
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• 1993

This study was attempted to establish the propagation and cultivation methods of Rhodiola elongata Fisch. et Mey. as a part of development of northern plant resources. Seeds were treated and investingated at the 15, 20, 25$^{\circ}C$ and 100, 200, 400, 800ppm of the growth regulator $GA_3$, BA, and Kinetin. The results obtained are summarized as follows: Seeds of R. elongata were the most vigorously germinated at 15-20$^{\circ}C$, and rate of germination at 25$^{\circ}C$ decreased gradually. Germination rate of seeds that treated growth regulators was remarkably increased than non-treated ones, and $GA_3$ treatment among the growth regulators showed the best effects. The effective concentration of $GA_3$ for germination of R. elongata seeds was 400 ppm.

• The Plant Pathology Journal
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• v.29 no.1
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• pp.93-98
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• 2013

Bacterial wilt is a vascular wilt disease caused by Xanthomonas translucens pv. poae that infects Poa annua, a grass that is commonly found on golf course greens throughout the world. Bacterial wilt causes symptoms of etiolation, wilting, and foliar necrosis. The damage is most prevalent during the summer and the pathogen can kill turf under conditions optimal for disease development. Fifteen isolates of X. translucens pv. poae were collected from northern regions in the United States and tested for virulence against P. annua. All 15 isolates were pathogenic on P. annua, but demonstrated variable levels of virulence when inoculated onto P. annua under greenhouse conditions. The isolates were divided into two virulence groups. The first group containing four isolates generally resulted in less than 40% mortality following inoculation. The second group, containing the other eleven isolates, produced between 90 and 100% mortality following inoculation. These results suggest that differences in the virulence of bacterial populations present on a golf course may result in more or less severe amounts of observed disease.

• Korean Journal of Plant Resources
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• v.11 no.2
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• pp.168-172
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• 1998

The morphlogical characteristics and cytotoxic acitivity for14 local varieties fo Codonopsis lanceolata collected from Korea were examined to get basic data for breeding and selection of good cultivars. Plant height ranged from 197.2 cm to 253.2 com, that from Beakunsan(No.11) was the highest and and that form Samcheok(No.5) was the lowest. Generally, plant height of those form northern area of Korea tends to be shorter than those from Southern area. Leaf width and length of that form Husando (No. 12) was the largest While that form chejudo(No.4) was the smallest and those from other areas showed no significant difference. As to the number of branches, that form Yanggu(No.1) was theleast and those from other areas did not show Chejudo(No.14) were the heaviest. Among 14 varieties tested, Heongseong(No.3) and Huksando(No.6) exhibited strong cytotoxicity , which showed 45.0 an d62.0$\mu\textrm{g}$. $m\ell$ respectively.

• Korean Journal of Pharmacognosy
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• v.28 no.4
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• pp.257-263
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• 1997

Berberis amurensis Ruprecht(Berberidaceae) is a medicinal plant indigenous to the middle and northern part of Korean peninsula. The woody parts of this plant have been used for the ocular, peptic and intestinal disorders. The stems of this plants were extracted with MeOH and the MeOH extract was partitioned between organic phases and water layer, successively to fractionated quarternary alkaloids. The acetone-soluble part of guarternary alkaloidal fraction had antibacterial activities and it contained four protoberberine alkaloids such as palmatine(I), Berberine(II), Jatrorrhizine(III) and coptisine(IV), and one aporphine alkaloid, magnoflorine(V). Although the isolations of the compounds I, II, IIII, IV and V from different sources were reported, this is the first report that Berberis amurensis contained the compounds. When the contents of compound I(palmatine) and II(berberine) were quantified and compared with those of other plant parts, cortex contained higher palmatine and berberine than any other part of the plant.

• Journal of Korean Society for Geospatial Information Science
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• v.25 no.2
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• pp.13-19
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• 2017

This paper analyzed MODIS data from 2006 to 2013 to determine relationship between meteorological changes and vegetation index. The experimental area was divided into the northern, central and southern regions according to the regional characteristics, and the smoothed MODIS LAI and NDVI were obtained using Timesat. In the case of precipitation, MODIS NDVI had correlation coefficients of 0.66, 0.44 and 0.35 in the northern, central and southern regions and the correlation was the highest in the northern region. In the case of temperature, MODIS LAI had correlation coefficients of 0.66, 0.64 and 0.68, and MODIS NDVI had 0.89, 0.89 and 0.80. The correlation of MODIS NDVI was higher and showed similar positive correlation regardless of region. In addition, The accuracy between Timesat plant seasonal start and actual plant seasonal start in MODIS NDVI was higher than MODIS LAI. The average error in MODIS LAI was 19 days in the central region and 20 days in the southern region. And the average error in MODIS NDVI was 6 days in the central region and 8 days in the southern region.

• Journal of Plant Biotechnology
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• v.4 no.4
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• pp.155-161
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• 2002

The HCV gene was expressed in potato plants under the control of the constitutive CaMV 355 promoter or tuber-specific patatin promoter. Solanum tuberosum plants carrying a plant expression vector harboring the encoding region of HCV gene were generated by Agrobacterium tumefaciens-mediated in vitro transformation methods. The presence of HCV gene in the plant genome was detected by PCR and DNA hybridization experiments. We obtained the 5 lines of transgenic potato with the pMBPHCV construct and 4 lines of transgenic potato with the pATHCV construct. The HCV transgenic stably integrated into the potato genome, as well as their transcription. HCV mRNA was identified in leaf and tuber tissues of transgenic plants by Northern blot analysis. The transgenic potato plants produced the expected transcript, and the corresponding HCV protein accumulated in individual transgenic plants.

• Journal of Plant Biotechnology
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• v.41 no.1
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• pp.50-55
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• 2014

Plant-based vaccines possess some advantages over other types of vaccine biotechnology such as safety, low cost of mass vaccination programs, and wider use of vaccines for medicine. This study was undertaken to develop the transgenic maize as edible vaccine candidates for humans. The immature embryos of HiII genotype were inoculated with A. tumefaciens strain C58C1 containing the binary vectors (V662 or V663). The vectors carrying nptII gene as selection marker and scEDIII (V662) or wCTB-scEDIII (V663) target gene, which code EIII proteins inhibite viral adsorption by cells. In total, 721 maize immature embryos were transformed and twenty-two putative transgenic plants were regenerated after 12 weeks selection regime. Of them, two- and six-plants were proved to be integrated with scEDIII and wCTB-scEDIII genes, respectively, by Southern blot analysis. However, only one plant (V662-29-3864) can express the gene of interest confirmed by Northern blot analysis. These results demonstrated that this plant could be used as a candidated source of the vaccine production.

• Journal of Plant Biotechnology
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• v.38 no.1
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• pp.42-48
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• 2011

Chloroplast genetic engineering of higher plants offers several unique advantages compared with nuclear genome transformation, such as high levels of transgene expression, a lack of position effect due to site-specific transgene integration by homologous recombination, multigene engineering in a single transformation event and reducing risks of gene flow via pollen due to maternal inheritance. We established a reproducible chloroplast transformation system of potato using a tobacco specific plastid transformation vector, pCtVG (trnI-Prrn-aadA-mgfp-TpsbA-trnA). Stable transgene integration into chloroplast genomes and the homoplasmic state of the transgenome were confirmed by PCR and Southern blot analyses. Northern, immunoblot analysis, and GFP fluorescence imaging revealed high expression and accumulation of GFP in the plastids of potato leaves. This system would provide new opportunities for genetic improvement and mass production of value added foreign proteins in this crop.

• Geomechanics and Engineering
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• v.6 no.5
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• pp.437-453
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• 2014

This paper studies the swelling and strength characteristics of unimproved and improved expansive soils in terms of the swell potential, swelling pressure, rate of secondary swelling, unconfined compressive strength and California bearing ratio (CBR). The admixtures used in this study are locally available cement and fly ash. The soils used in this study were taken from the Mae Moh power plant, Lampang Province, in northern Thailand. A conventional consolidation test apparatus was used to determine the swelling of the soil specimen. The optimum admixture contents are determined to efficiently reduce the swelling of unimproved soil. The rate of secondary swelling for unimproved soil is within the range of highly plastic montmorillonite clay, whereas the specimens improved with optimum admixture contents can be classified as non-swelling kaolinite. A soil type affects the swelling pressure. Expansive soil improvement with fly ash alone can reduce swelling percentage but cannot enhance the unconfined compressive strength and CBR. The strength and swelling characteristics can be predicted well by the swelling percentage in this study.

• Plant Resources
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• v.5 no.1
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• pp.78-85
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• 2002

The material of Rhodiola sachatinensis collected from an alpine region of the west-northern China. For analysing the effect, 1 used Rhodiola sachatinensis's rhizome and cultivated callus. In EtOAc, BuOH, $H_2O$ separation the plant showed strong antioxidative activity, but not in Hexane. The radical scavenging effect of EtOAc(RC$_{50}$,35(g), BuOH(RC$_{50}$, 43(g), H$_2$0(RC$_{50}$, 50(g) fraction and MeOH extract(RC$_{50}$, 50(g) of the Rhodiola sachatinensis was comparable to that of synthetic antioxidant BHA(RC$_{50}$, 14(g) and $\alpha$-Tocopherol(RC$_{50}$, 12(g). Total amino acid concentration of plant of In nature condition were 18,009ppm, and major components were arginine, glutamic acid, aspartic acid and valine. The ratio of essential/total amino acid on plant of In nature condition was 46.93%. Total amino acid concentration of callus of In vitro condition were 32,435ppm, and major components were valine, histidine, lysine and leucine. The ratio of essential/total amino acid on callus of In vitro condition was 56.07%. was 56.07%.