• Title/Summary/Keyword: normal structure

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Function and Oligomerization Study of the Leucine Zipper-like Domain in P13 from Leucania separata Multiple Nuclear Polyhedrosis Virus

  • Du, Enqi;Yao, Lunguang;Xu, Hua;Lu, Songya;Qi, Yipeng
    • BMB Reports
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    • v.40 no.2
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    • pp.232-238
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    • 2007
  • The p13 gene is uniquely present in Group II nucleopolyhedroviruses (NPVs) and some granuloviruses, but not in Group I NPVs. p13 gene was first described by our laboratory in Leucania separatamultiple nuclear polyhedrosis virus (Ls-p13) in 1995. However, the functions of Ls-P13 and of its homologues are unknown. When Ls-p13 was inserted into Autographa californica nucleopolyhedrovirus, a Group I NPV, polyhedra yield was inhibited. However, this inhibition was prevented when the leucine zipper-like domain of Ls-p13 was mutated. To determine the cause of this marked difference between Ls-P13 and leucine zipper mutated Ls-P13 (Ls-P13mL), oligomerization and secondary structure analyses were performed. High performance liquid chromatography and yeast two-hybrid assays indicated that neither Ls-P13 nor Ls-P13mL could form oligomers. Informatics and circular dichroism spectropolarimetry results further indicated marked secondary structural differences between Ls-P13 and Ls-P13mL. The LZLD of Ls-P13 has two extended heptad repeat units which form a hydrophobic surface, but it is short of a third hydrophobic heptad repeat unit for oligomerization. However, the mutated LZLD of Ls-P13mL lacks the above hydrophobic surface, and its secondary structure is markedly different. This difference in its secondary structure may explain why Ls-P13mL is unable to inhibit polyhedra yield.

Structure of Opposite Wood in Angiosperms(II) - Structure of Opposite Woods in the Horizontal-growing Stems of Immature Woods - (활엽수(闊葉樹) Opposite재(材)의 구조(構造)(II) - 수평(水平) 생장(生長)시킨 유영목수간(幼 令木樹幹)의 Opposite재(材) 구조(構造) -)

  • Park, Sang-Jin;Park, Byung-Soo
    • Journal of the Korean Wood Science and Technology
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    • v.17 no.3
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    • pp.20-27
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    • 1989
  • This experiment was made to find the peripheral variations of annualring widths, the cell dimensions, microfibril angles and bulk densities within each annual-ring of horizontal-growing young tree of beech(Betul a platyphylla var. japonica) and Oak (Quercus variabilis) from the tension to the opposite side. Also a comparision between the features of the obnormal annual ring for horizontal-growing year and normal annual ring for the straight-growing years was made. The dimension of propotion of the element, the microfibril angles and the bulk density decreased or increased continuously toward opposite side which showed minimum or maximum value. The dimension of elements the microfibril angles and the bulk density decreased or increased continuously towards opposite side which showed minimum or maximum value. The dimension of elements. the microfibril angles and the bulk density in the normal annual rings were similar to those in the lateral woods. whereas were significantly more different in the tension wood than in the opposite wood. The features of typical opposite wood in the hardwoods were influenced by the locations within the inclined stems than effects of the decrease in the annual ring width. The oppostie woods in hardwoods did not conform to the tension wood and lateral wood. The abnormal annual ring included the opposite wood, lateral wood similar to normal wood and tension wood having specialized structure even in the same annual ring.

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Enzyme-Resistant Starch Formation from Mild Acid-Treated Maize Starches (약산처리 옥수수전분으로 호소저항전분의 생성)

  • Lee, Shin-Kyung;Mun, Sae-Hun;Shin, Mal-Shick
    • Korean Journal of Food Science and Technology
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    • v.29 no.6
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    • pp.1309-1315
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    • 1997
  • Yields of enzyme-resistant starch (RS) from three kinds of maize starches (Amioca, normal starch and Amylomaize VII) which were treated with 1 N HCl for 24 hr and physicochemical characteristics were investigated. Hydrolysis rate of maize starches decreased with increasing amylose content. Maximum wavelength $({\lambda}_{max})$ and iodine affinity were decreased by the acid treatment. The yields of RS increased with acid treatment up to 12 hr and then decreased. The yield of for 12 hr acid-treated Amioca increased 8 times more than untreated sample, but those of normal starch and Amylomaize VII slightly increased. Using SEM, acid-treated and autoclaved maize starches showed gel like structure, but RS had round and rod shape small particles. X-ray diffraction patterns of autoclaved starches showed amorphous structure in Amioca and B-type in normal starch and Amylomaize VII, and those of RS showed all completely crystalline structure.

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Synthesis and Characterization of pH-sensitive and Self-oscillating IPN Hydrogel in a pH Oscillator (pH 진동계 안에서 pH 감응성 자기진동 IPN 하이드로젤의 합성과 분석)

  • Wang, Liping;Ren, Jie;Zhang, Xiaoyan;Yang, Xiaoci;Yang, Wu
    • Polymer(Korea)
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    • v.39 no.3
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    • pp.359-364
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    • 2015
  • A self-oscillating interpenetrating polymer network (IPN) poly(acrylic acid)/poly(ethylene glycol) (PAA/PEG) hydrogel was prepared by using radical polymerization with a two-step method. The IPN hydrogel was characterized by FTIR spectroscopy and morphological analysis. The results indicated that the chains of PEG and PAA twined to form porous structure which is beneficial to water molecules entering inside of the hydrogel. In addition, the pH-responsive behavior, salt sensitivity, swelling/de-swelling oscillatory behaviors and self-oscillation in a closed pH oscillator were also studied. The results showed that the prepared hydrogel exhibited pH-sensitivity, good swelling/de-swelling reversibility and excellent salt sensitivity. The self-oscillating behavior of swelling/de-swelling for the prepared hydrogel was caused by pH alteration coupled with the external media. This study may create a new possibility as biomaterial including new self-walking actuators and other related devices.

Interpretation of geologic structure in Tertiary Pohang basin, Korea (포항분지내 지각변형 해석)

  • Lee, Byung-Joo;Song, Kyo-Young
    • Economic and Environmental Geology
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    • v.28 no.1
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    • pp.69-77
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    • 1995
  • Tertiary Pohang basin distributed in south western part of the Korean peninsula, is composed of Chunbuk formation as the basal conglomerate, Hakjon formation, Duho formation and intrusive basalt having 15 Ma by absolute age data. The basement of the basin is represented to Cretaceous sedimentary rocks, Hakjon welded tuff and Chilpo welded tuff and rhyolite. The fault systems in the basement of Tertiary Pohang basin are consist of $N20^{\circ}E$ fault, $N60^{\circ}W$ and E-W trend. NNE fault is not only strike-slip but also normal dip-slip. WNW fault has sinistral strike-slip sense and the geometry of E-W fault is strike-slip and normal faults. In the basin, the fault system is represented to $N20^{\circ}E$ strike-slip, E-W normal and NNE thrust faults. By these fault relationship and geometry, it is interpreted that NNE sinistral strike-slip fault and N-S normal faults have acted at the Cretaceous basement. After Miocene NNE dextral strike-slip fault has acted and created E-W normal fault. Progressively Tertiary basin was influenced by the transpression to make thrust and fold, namely inversion tectonics.

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Normal Mode Approach to the Stability Analysis of Rossby-Haurwitz Wave

  • Jeong, Hanbyeol;Cheong, Hyeong Bin
    • Journal of the Korean earth science society
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    • v.38 no.3
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    • pp.173-181
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    • 2017
  • The stability of the steady Rossby-Haurwitz wave (R-H wave) in the nondivergent barotropic model (NBM) on the sphere was investigated with the normal mode method. The linearized NBM equation with respect to the R-H wave was formulated into the eigenvalue-eigenvector problem consisting of the huge sparse matrix by expanding the variables with the spherical harmonic functions. It was shown that the definite threshold R-H wave amplitude for instability could be obtained by the normal mode method. It was revealed that some unstable modes were stationary, which tend to amplify without the time change of the spatial structure. The maximum growth rate of the most unstable mode turned out to be in almost linear proportion to the R-H wave amplitude. As a whole, the growth rate of the unstable mode was found to increase with the zonal- and total-wavenumber. The most unstable mode turned out to consist of more-than-one zonal wavenumber, and in some cases, the mode exhibited a discontinuity over the local domain of weak or vanishing flow. The normal mode method developed here could be readily extended to the basic state comprised of multiple zonalwavenumber components as far as the same total wavenumber is given.

Characterization, Antioxidant Capacity and Protective Effect of Peptides from Cordyceps militaris Cultivated with Tussah Pupa on Oxidative Injured HepG2 Cells

  • Bingxin Li;Jinying Zhang;Yefei Liu;Ze Wang;Fangxu Xu
    • Journal of Microbiology and Biotechnology
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    • v.34 no.5
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    • pp.1082-1091
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    • 2024
  • The antioxidant capacity and protective effect of peptides from protein hydrolysate of Cordyceps militaris cultivated with tussah pupa (ECPs) on H2O2-injured HepG2 cells were studied. Results indicated ECP1 (<3 kDa) presented the strongest antioxidant activity compared with other molecular weight peptides. Pretreated with ECPs observably enhanced survival rates and reduced apoptosis rates of HepG2 cells. ECPs treatment decreased the ROS level, MDA content and increased CAT and GSH-Px activities of HepG2 cells. Besides, the morphologies of natural peptides from C. militaris cultivated with tussah pupa (NCP1) and ECP1 were observed by scanning electron microscopy (SEM). Characterization results suggested the structure of NCP1 was changed by enzymatic hydrolysis treatment. Most of hydrophobic and acidic amino acids contents (ACC) in ECP1 were also observably improved by enzymatic hydrolysis. In conclusion, low molecular weight peptides had potential value in the development of cosmetics and health food.

A Study on the Flame Pattern and the Electrical Properties of Electric Outlet Fired at Standby Mode (Standby Mode에서 출화된 콘센트의 화염 패턴 및 전기적 특성에 관한 연구)

  • 최충석;송길목;김형래;김향곤;김동욱;김동우
    • Fire Science and Engineering
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    • v.16 no.1
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    • pp.39-44
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    • 2002
  • In this paper, we analysed the flame patterns and the electrical characteristics of the electric outlet which was fired at standby mode. The carbonized patterns indicated that the flame had spread about 50 cm to 70 cm. After the combustibles on wall started to burn, the temperature went up to about $300^{\circ}c$ in 150 sec. The flame formed ceiling jet and spread quickly. The tracking was generated at the shortest distance between two electrodes and the resistance was about 100$\Omega$ to 300$\Omega$ As the result of analysis using metallurgical microscope, the normal part of a blade holder showed amorphous structure, but the melted part of a blade holder damaged by tracking showed dendrite structure and void evenly. When the blade holder of damaged outlet was analyzed by SEM and EDX, we found that the structure and components of the normal part were different from those of melted part.

Vibration Isolation Characteristics of CRP Materials and SNORE Ring on the Multi-Curved Structure (CRP 재질 및 SNORE 링 부착에 따른 다층 곡면 구조물의 진동 차단 특성 연구)

  • Lee, Jong-Kil;Jo, Chi-Yong
    • 대한공업교육학회지
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    • v.35 no.2
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    • pp.224-237
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    • 2010
  • When the underwater structure sails high speed, noise and vibration propagate to the sensor in the nose of the dome. In this paper, to avoid this kind of noise and vibration CRP(Carbon Reinforced Plastic) material and SNORE ring(Self-NOise REduction Ring) are attached at the curved structure and simulates its isolation characteristics using commercial software. Vibration displacement and stress are calculated at the planar sensor array. The material of the curved structure is aluminum and maximum outer diameter is 53Omm, 215mm in length, 270mm in planar diameter, respectively. Based on the simulation results, reduction ratio of the received normal stress at the sensor is above 95% at the frequency of 12kHz and 15kHz. At the mid point of the planar sensor the normal stress is higher than 20mm and 40mm apart. This results can be used to increase the sensitivity of the acoustic sensor as a basic data.

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Analysis of Chorion Structures in Kidney Mutant, Bombyx mori (신장형란에 있어서 난각구조의 해석)

  • 노시갑
    • Journal of Sericultural and Entomological Science
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    • v.30 no.2
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    • pp.88-95
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    • 1988
  • The structure ki mutant chorion is described by the ultrastructural level with the scanning electron microscope. The micropyle on the anterior, posterior, leteral and dorsal side region surface patterns do not so much differ from ki mutant and normal strains. The surface structure of ventral side of ki mutant, which is the presumptive embryonic region in oocytes, were netted part corresponding to the boundary of follicle cells that is similar to lateral side structure of egg-shell. The eggshell thickness of ventral side on the ki mutant is mostly thicker. However, the structure of transverse section of normal and ki mutant egg-shell are composed of the outer, middle and inner layers. The ki not effects the formation of the lamellar layer in the egg-shell. It is observed that the characteristic structure in ki mutant egg chorion may be attributed to a areal specificity of epithelium in ventral side of egg-shell formation, and it affects the process of the accumulation of secreted chorionic material and the egg-shell architecture.

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