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In Vitro Flowering System (In Vitro 시스템에 의한 화호형성)

  • 류장렬;이행순;이광웅
    • Proceedings of the Botanical Society of Korea Conference
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    • 1987.07a
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    • pp.213-237
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    • 1987
  • In vitro flowering system may minimize the confounded influence of non-floral meristem parts of plants in studying the relationship of a given treatment and flowering responses. We have induced flower buds from plantlets regenerated from zygotic embryo-derived somatic embryos of ginseng, which circumvented the normal 2-year juvenile period before flowering. The result suggests that the adulthood of ginseng root explants in the experiment previously conducted by Chang and Hsing (1980; Nature 284: 341-342) is not prerequired to flowering of plantlets regenerated through somatic embryogenesis. We have also induced flower buds from elongated axillary brandches from cotyledonary nodes by culturing ginseng zygotic embryos, seedlings, and excised cotyledonary nodes. It was found that 6-benzyladenine (BA) supplemented to the medium was essential for flowering, whereas abscisic acid (ABA) was inhibitory. Gibberellic acid(GA3) was also required for flowering when ABA was present with BA in the medium. The results suggest that cytokinins, gibberellins, and inhibitors play primary, permissive, and preventive roles, respective-ly, in the induction of flowering of ginseng. Tran Thanh Van (1980; Int. Rev. Cytol., Suppl. IIA: 175-194) has developed the "thin cell layer system" in which the induction of shoots, roots, or flower buds from epidermal layer explants were controlled by culture conditions and exogenous growth regulators in the medium, Utilizing the thin cell layer system, Meeks-Wagner et al. (1989; The Plant Cell 1: 25-35) have cloned genes specifically expressed during floral evocation. However, the system is too tedious for obtaining a sufficient amount of plant materials for biochmical and molecular biological studies of flowering. We have developed a garlic callus culture system and one obvious advantaging over the thin cell layer system is that an abundant cells committed to develope into flower buds proliferate. When the above cells were compared by two-dimensional gel electrophoresis with those which have just lost the competence for developing into flower buds, a few putative proteins specific to floral evocation were detected. The garlic callus culture system can be further explored for elucidation of the molecular biological mechanism of floral evocation and morphogenesis.hogenesis.

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Contralateral recurrence of necrotizing sialometaplasia of the hard palate after five months: a case report

  • Jeong, Chan-Woo;Youn, Taegyun;Kim, Hyun Sil;Park, Kwang-Ho;Huh, Jong-Ki
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
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    • v.41 no.6
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    • pp.338-341
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    • 2015
  • Necrotizing sialometaplasia usually heals within 4 to 10 weeks with conservative treatment, and rarely recurs. When necrotizing sialometaplasia is present on the hard palate it may occur unilaterally or bilaterally. In this case, necrotizing ulceration occurred on the left hard palate of a 36-year-old woman after root canal treatment of the upper left first premolar under local anesthesia. After only saline irrigation the defect of the lesion completely healed and filled with soft tissue. After 5 months, however, a similar focal necrosis was found on the contralateral hard palate without any dental treatment having been performed on that side and progressed in similar fashion as the former lesion. We conducted an incisional biopsy and obtained a final pathological diagnosis for the palatal mass of necrotizing sialometaplasia. At the 3-year follow-up, the patient's oral mucosa of the hard palate was normal, without any signs and symptoms of the condition. We report a case of a second occurrence of necrotizing sialometaplasia on the contralateral side from the first, with a time lapse between the first and second occurrence.

ACC Deaminase Producing Arsenic Tolerant Bacterial Effect on Mitigation of Stress Ethylene Emission in Maize Grown in an Arsenic Polluted Soil

  • Shagol, Charlotte C.;Subramanian, Parthiban;Krishnamoorthy, Ramasamy;Kim, Kiyoon;Lee, Youngwook;Kwak, Chaemin;Sundaram, Suppiah;Shin, Wansik;Sa, Tongmin
    • Korean Journal of Soil Science and Fertilizer
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    • v.47 no.3
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    • pp.213-216
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    • 2014
  • Arsenic is a known hazardous metalloid not only to the animals but also to plants. With high concentrations, it can impede normal plant growth and cause even death of plants at extremely high levels. A known plant response to stress conditions such as toxic levels of metal (loids) is the production of stress ethylene, causing inhibitory effect on root growth in plants. When the effect of various arsenic concentrations was tested to maize plant, the stress ethylene emission proportionately increased with increasing concentration of As(V). The inoculation of two arsenic tolerant bacteria; Pseudomonas grimonti JS126 and Pseudomonas taiwanensis JS238 having respective high and low 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity reduced stress ethylene emission by 59% and 30% in maize grown in arsenic polluted soils. The result suggested the possible use of Pseudomonas grimonti JS126 for phytoremediation of arsenic polluted soils.

The effect of advanced periodontitis on the dental pulp tissue (중증치주염이 치수조직에 미치는 영향)

  • Kim, Byung-Ock;Park, Young-Ran;Yoon, Jung-Hoon;Jang, Hyun-Seon
    • Journal of Periodontal and Implant Science
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    • v.35 no.2
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    • pp.311-319
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    • 2005
  • In order to examine the effects of advanced periodontitis on the dental pulps, 38 extracted human teeth were examined histologically. The 38 teeth had a positive or negative state in the electric pulp test(EPT). In addition, almost of the 38 teeth had a deep pocket and severe mobility, and floating state. A medical and dental history was elicited. The extracted teeth fixed in 10% neutral formalin solution. The general tissue processing method was followed. The tissue block including the teeth was prepared for optical microscopy using hematoxillin-eosin staining. Among the 38 periodontally involved teeth, the dental pulps were respectively intact in 12(31%), and a pulp stone(or linear calcifications) was found in 18 teeeth(47%). In addition, 17 teeth(44%) had pulps exhibiting inflammatory reactions with varying intensities, such as hyperemia, pulp abscess, pulp necrosis. Among the 38 periodontally involved teeth, 37 teeth tested a positive to the EPT, and 7 teeth tested negative. The EPT positive 37 teeth had various histological features such as 7 normal pulp(18%), 17 pulp stone(44%), 1 hyperemia (2%), 9 pulpitis(23%), 5 root resorption(13%), 3 pulp abscess(7%), and 3 pulp necrosis(7%), In conclusion, it is suggested that in the EPT positive teeth, advanced periodontally involved teeth can cause inflammation of the dental pulp.

Periodontal treatment of a Glanzmann's thrombasthenia patient : A case report (Glanzmann씨 혈소판무력증(Glanzmann's Thrombasthenia) 환자의 치주 치료 증례)

  • Lee, Hak-Churl;Han, Soo-Boo;Kim, Woo-Sung;Lee, Hye-Ja
    • Journal of Periodontal and Implant Science
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    • v.27 no.3
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    • pp.597-602
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    • 1997
  • Glanzmann's thrombasthenia is a Qualitative platelet disorder characterized by a deficiency in the platelet membrane glycoproteins IIb/IIIa. It belongs to a group of hereditary platelet disorders typified by normal platelet numbers and a prolonged bleeding time. The severity of bleeding does not correlate with the severity of the platelet glycoprotein IIb/IIIa a abnormality. The present case report describes the periodontal treatment of a patient with Glanzmann's thrombasthenia. A 30-year-old female with a history of Glanzmann's thrombasthenia was referred for gingival bleeding on tooth brushing and discomforts in #38 area. The periodontal finding revealed a diagnosis of localized slight adult periodontitis. Root planing and extraction of #38 was performed under 12 pack of platelets transfusion and digital compression was done for hemostasis. The gingival bleeding ceased within a day in maxilla and 2 days later in mandible. 42 pack of platelets was administered for 3 days of post-treatment and for iron-deficiency anemia 3 pack of RBCs was transfused 2 days later. 1 week later the inflammation in gingiva disappeared and gingival stippling appeared. The clinical result we got was good and in such a medically compromised patient it is an ability to maintain a proper oral hygiene that is essential both for oral and systemic health.

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A literature study on Bromidrosis (腋격의 病因.病機.治療에 對한 文獻的 考察)

  • Kim, Jong-sung;Kim, Kyung-joon
    • The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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    • v.13 no.1
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    • pp.157-184
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    • 2000
  • Bromidrosis is a disease which is also called huchou[호격] in the oriental medicine. This disease produces an odor and a small amount of sweat from the axillary area. Prognosis of the disease could possibly get better with age. This can be explained by the fact that its symptoms begin usually when a person reaches puberty and as the person gets old, the symptoms reduce or disappear even when specific treatments are not applied. Although the disease is not lethal, many patients have difficulties in leading a normal and comfortable life, physically and psychologically with this in mind and some osbservations gained from the literature studies on the causes,symptoms, and treatments, I embarked on a study aimed at finding out the therapies. My study on the literature has produced the following results ; 1. In the oriental medicine, hu-chou[호격] means the characteric smell or odor produced from the fox. 2. The literature studies point out two causes in the oriental medicine. One is natural. The other is acquired. Acquired cases have the following causes: shi-re-nei-yun[濕熱內蘊]. xie-뱌-bu-huo[血氣不和], gan-qi-yu-jie[肝氣鬱結]. 3 There are two therapies in the oriental medicine. One is internal and the other external. out of the two, external therapies were mainly used according to the literature studies that I have consulted. 4. According to my study, the most frequently used herbal medicine was Alunitum(明 礬)(45 times), followed by Minium(密陀僧)(39 times) and Moschus(?香)(21 times respectively). 5. My study also found that bian-zheng[辨證] was not .rarely listed on old literatures. Therefore, old literatures did not clearly show how to root out the disease. They only taught now to alleviate the symptoms and remove the characteric odor.

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Efficient Bulblet Regeneration and Growth from Bulb Scale of Hyacinthus orientalis L. cv. Pink Pearl Cultured in vitro (히아신스(cv. Pink Pearl)의 인편 기내 배양시 효과적인 자구의 재생과 생장)

  • Lee, Kyung-Soon;Chung, Chung-Han;Chung, Yong-Mo;Kim, Doh-Hoon;Jeong, Soon-Jae;Nam, Jae-Sung;Kim, Gyung-Tae;Yi, Young-Byung
    • Journal of Life Science
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    • v.17 no.10
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    • pp.1336-1340
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    • 2007
  • The regeneration and growth of bulblets from the bulb scale segments of Hyacinthus orientalis L. cv. Pink Pearl were more efficient in IBA than IAA at the same concentrations (1.0 and 3.0 mg/l). The normal (base-down) orientation of explants was more effective for bulblet regeneration and root growth than the inverted (base-up) orientation. The growth of bulblets and roots was increased higher in the perlite than the agar medium. These results suggested that the alternate culture system, first cultured in the agar medium for bulblet regeneration, and then in the perlite medium for bulblet growth, may be more useful for efficient in vitro culture of hyacinth (H. orientalils) cv. Pink Pearl.

Malignant Proliferating Trichilemmal Tumor - A Case Report - (악성 증식성 모낭 종양 - 증례 보고 -)

  • Hong, Ki-Do;Kim, Jae-Young;Ha, Sung-Sik;Sim, Jae-Chun;Cho, Hye-Jae;Choi, Yoon-Ho
    • The Journal of the Korean bone and joint tumor society
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    • v.15 no.1
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    • pp.65-68
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    • 2009
  • Proliferating trichilemmal tumor(PTT) is a rare neoplasm derived from the outer root sheath of the hair follicle. Malignant transformation is rare, but abrupt enlargement of size, infiltrative growth, and non-scalp location are suggestive of malignant PTT. Histopathologic characteristics of malignant PTT are nuclear atypia, pleomorphism, and mitotic figures. Recurrence and metastasis have been documented in malignant PTT. Wide resection of the tumor with the normal tissue and accurate follow-up is the treatment of choice of the both malignant and benign PTT. We report a 51-year-old man with the mass on right 5th finger distal phalanx for 7 years. The tumor was excised with middle phalangiectomy, and based on the histopathologic findings of the tumor, this case was diagnosed as mailignant PTT. We cannot detect recurrence and metastasis because the patient was lost to follow-up.

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Analysis of Angular Velocity during Toe Tapping for the Quantification of the Lower Limb Bradykinesia in Patients with Idiopathic Parkinson's Disease (특발성 파킨슨병 환자의 하지 완서증 정량화를 위한 발 두드리기 동작의 각속도 분석)

  • Kim, Ji-Won;Kwon, Yu-Ri;Eom, Gwang-Moon;Kim, Hyung-Sik;Yi, Jeong-Han;Kwon, Do-Young;Koh, Seong-Beom;Park, Byung Kyu;Kwon, Tae-Kyu
    • The Transactions of The Korean Institute of Electrical Engineers
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    • v.59 no.11
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    • pp.2114-2118
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    • 2010
  • The purpose of this study was to analyze bradykinesia of toe tapping movement in patients with Parkinson's disease (PD) as compared to those of normal subjects. 39 PD patients (age: $65.5{\pm}11.2$ yrs, H&Y stage:$2.3{\pm}0.5$), 14 eldelry subjects with comparable mean age ($65.0{\pm}3.9$ yrs) and 17 healthy young subjects ($24.1{\pm}2.1$ yrs) participated in this study. Angular velocity during repetitive toe tapping movement was measured in both feet using a gyro sensor system. Suggested quantitative measures of bradykinesia were root-mean-squared (RMS) angular velocity, RMS angle, peak power and total power which were derived from the angular velocity signal. ANOVA showed that all measures were significantly different among three groups (p<0.001). Subsequent post-hoc test revealed that all measures in PD patients were significantly smaller than in healthy elderly and healthy young subjects (p<0.02). All measures were significantly correlated with UPDRS scores(r=-0.689~-0.825). These results suggest that the developed system can be used as quantitative measures of the lower limb bradykinesia in PD patients.

Classification of walking patterns using acceleration signal (가속도 신호를 이용한 걸음걸이 패턴 분류)

  • Jo, Heung-Kuk;Ye, Soo-Young
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.14 no.8
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    • pp.1901-1906
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    • 2010
  • This classification of walking patterns is important and many kinds of applications. Therefore, we attempted to classify walking on level ground from slow walking to fast walking using a waist acceleration signal. A tri-axial accelerometer was fixed to the subject's waist and the three acceleration signals were recorded by bluetooth module at a sampling rate of 100 Hz eleven healthy. The data were analyzed using discrete wavelet transform. Walking patterns were classified using two parameters; One was the ratio between the power of wavelet coefficients which were corresponded to locomotion and total power in the anteroposterior direction (RPA). The other was the ratio between root mean square of wavelet coefficients at the anteroposterior direction and that at the vertical direction(RAV). Slow walking could be distinguished by the smallest value in RPA from other walking pattern. Fast walking could be discriminated from level walking using RAV. It was possible to classify the walking pattern using acceleration signal in healthy people.