• Microbiology and Biotechnology Letters
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• v.27 no.1
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• pp.23-27
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• 1999

The development and industrial application of intestinal anaerbic bacteria is highly important in the munufacture of foodstuffs and medical products. We have conducted studies, screening anaerobic intestinal bacteria for acid and bile tolerance and for pathogens suppression in the intestine of swine. One such study was taxonomic in nature and dealt with the isolate's biochemical responses. The results indicated that the BSA-131 strian ioslated. which suppressed various pathogens (13 typical strains), were identified as Lactobacillus reuterii. The strain, which named L. reuterii BSA-131, was able to tolerate acid condition down to pH2 and could also tolerate bile at 5%. The strain exhibited resistance to a range of antibiotics including, cephalexin, erythromycin, flumequine, furazolidine, gentamycin, penicillin G, norfloxacin, spectinomycin, tetracycline, tiamuline, neomycin, chloramphenicol, kanamycin etc.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.41 no.3
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• pp.188-192
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• 2008

Methyl gallate isolated from bark of the tree Rhus verniciflua Stokes has significant antimicrobial activity against the fish pathogenic bacteria Edwardsiella tarda and Vibrio anguillarum. To evaluate the antimicrobial activity of gallate derivatives, eight alkyl gallates were tested. Ethyl gallate and propyl gallate had the highest activities, with MICs of $15.6-31.3{\mu}g/mL$ against E. tarda. For V. anguillarum, propyl gallate and butyl gallate were highly effective, with MICs of $7.81-31.3{\mu}g/mL$. When used in combination with antibiotics, methyl gallate exhibited synergistic effects with oxytetracycline against E. tarda and with norfloxacin against V. anguillarum. These results suggest that short-chain alkyl gallates can be used as alternatives to antibiotics against the fish pathogenic bacteria.

• Journal of Veterinary Clinics
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• v.18 no.2
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• pp.98-104
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• 2001

The present study was conducted to investigate isolation and antimicrobial drug susceptibility of Pasteurella spp from pneumonic calves and cows (pneumonic lungs) in Gyeongnam south area from January to November 2000. Pasteurella multocida and P haemolytica were isolated 136 (53.8%) and 22(8.7%), respectively, from 158 of 253 pneumonic calves of 1 to 12 months of age and from pneumonic lungs and simultaneous isolation rate of P mutocida and P haemolytica was 4 (1.6%). Seasonal isolation rate of Pasteurella spp in calves and pneumonic lungs varied from 3 to 65.2%, and it was higher in Fall. The majority of biochemical and cultural properties of P multocida and P haemolytica isolated from calves and pneumonic lung were identical to those of the reference strains employed. Pneumonic lesions of the examined lungs were histologically identified as purulent bronchopneumonia and fibrinous pneumonia. All isolates were resistant to penicillin, amikacin, lincomicin, colistin, cloxacilling, sulfonamide, kanamycin, sulfamethoxasoletrimetoprim, and gentamicin(>80%), but some of them were susceptible to amoxicillin, norfloxacin, cephalexin, cefazolin, orbifloxacin, enrofloxacin, and ciprofloxacin(>60%).

• Korean Journal of Veterinary Service
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• v.23 no.1
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• pp.77-91
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• 2000

This study was conducted to investigate the antibiotic resistance and plasmid profiles of 58 salmonella spp isolated from mesenteric lymphnodes of slaughter pigs in Kyoungbuk province during the period from September 1997 to June 1998. The results obtained are as follow that all isolates were susceptible to amikacin, gentamicin, ciprofloxacin and enrofloxacin, and the majority of isolates were highly susceptible to norfloxacin, colistin, nalidixic acid and apramycin while they were moderately susceptible to kanamycin, cephalothin, chloramphenicol, ampicillin, trimethoprim and penicillin. The majority of isolates were over 90% resistant rates to lincomycin, sulfadimethoxine, vancomycin, methicillin and erythromycin. The plasmid profiles of 58 salmonella spp are developed 1 to 4 fractions, 0.9 to 29.5 Kb molecular range sizes and U strains (45.5%) were showed plasmid profiles by agarose gel electrophoresis. 5 derby harbored 29.5 Kb and 7 Kb, and S schwarzengrund had 14 Kb and 0.9 Kb harboring sizes. Four of 10 S agona and 2 of 4 S typhimurium were harbored 3.1 Kb and n.5 Kb, respectively. Thirty-five untypable strains are developed variable size fractions its showed small size plasmid profile less than 6 Kb and 22 (62.8%) of them had no detectable plasmids.

• Korean Journal of Veterinary Service
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• v.27 no.3
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• pp.257-263
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• 2004

This study were carried out to investigate isolation of Pasteurella multocida from respiratory disorder piglets, to examine the biochemical properties and antimicrobial susceptibility. The results were summarized as follows; P multocida was isolated from 31($10.3\%$) of the 302 respiratory disorder or growing piglets of $4{\sim}10$ week olds. The majority of biochemical and cultural properties of the P multocida isolates were identical to those of the standard strains. The isolates were highly susceptible to norfloxacin($100.0\%$), enrofloxacin($96.8\%$) and ampicillin($87.1\%$), but resistant to streptomycin ($77.4\%$), penicillin($61.2\%$) and neomycin($54.8\%$).

• Korean Journal of Veterinary Research
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• v.50 no.3
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• pp.231-237
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• 2010

This study was conducted to investigate characteristics and antimicrobial susceptibility of Escherichia (E.) coli isolates isolated from vaginal mucosa and clitorial fossa of 105 Thoroughbred mares suspicious of the genital disease in Korea during the period from March 2006 to July 2007. Ninety six E. coli isolates were identified as standard biochemical properties and using BIOLOG system. Fifty three isolates (55.2%) could be classified into a total of 21 O serotypes and forty three isolates (44.8%) were non-typeable with 51 O antisera used in this study. The verotoxin 1 (VT 1) and verotoxin 2 genes were analyzed by multiplex PCR. Among them, one isolate was detected VT 1 gene (130 bp). Most of isolates showed a high susceptibility in ciprofloxacin (100%), enrofloxacin (100%), norfloxacin (100%), cefoxitin (96.9%), gentamicin (96.9%), sulphamethoxazole (96.9%), nitrofurantoin (94.8%), amikacin (93.8%), nalidixic acid (92.7%) and tetracycline (90.6%). These results may provide the basic information to establish strategies for the treatment and prevention of reproductive disease in Thoroughbred mares in Korea.

• Journal of fish pathology
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• v.16 no.1
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• pp.61-68
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• 2003

MICs of 8 chemotherapeutic agents against forty streptococcal isolates were determined. These strains were isolated from diseased olive flounder, Paralichthys olivaceus, and showed 2-5 multiple drug resistance against different antibacterial agents including ampicillin, doxycycline (DOXY), erythromycin, florfenicol, flumequine, norfloxacin, oxolinic acid, and oxytetracycline (OTC). In conjugation experiment, we found transferable R plasmids carrying OTC and DOXY resistance determinant in 3 drug resistance strains analyzed. Six out of 40 isolates showed positive signal in colony hybridization with the R plasmid DNA (pST9) as a probe. It suggests that other types R plasmid different from pST9 is also involving in multiple drug resistance of streptococci isolated from olive flounder.

• Mycobiology
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• v.29 no.1
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• pp.11-14
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• 2001

A preparation of water soluble components(EA) was made from carpophores of Elfvingia applanata(Pers.) Karst and its in vitro antibacterial activity on a number of bacterial species was examined by macrobroth dilution assay. Among 16 species of bacteria tested, the most potent antibacterial activity was observed against Staphylococcus epiderrnidis and Proteus vulgaris, of which MICs were 1.25 mg/ml. To investigate the antibacterial effects in combinations of EA with quinolone antibiotics, such as ciprofloxacin, enoxacin, lomefloxacin, norfloxacin, and ofloxacin, the fractional inhibitory concentrations(FICs) and the fractional inhibitory concentration indices(FICIs) for four bacterial strains were determined by macrobroth dilution checkerboard assay. Combinations of EA and quinolones exhibited either additive or indifferent effects of antibacterial activity in most instances. However, both synergistic and antagonistic effects were not observed in any cases.

• Korean Journal of Microbiology
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• v.38 no.4
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• pp.265-265
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• 2002

Iron increased the susceptibilities of clinical isolates Pseudomonas aeruginosa to quinolones. In the presence of iron, increased susceptibilities to ofloxacin were observed in twenty-six out of thirty isolates and with no change in four isolates. In the case of norfloxacin, iran increased susceptibilities of twelve isolates but did not render any change in eighteen isolates. In the case of ciprofloxacin, iron decreased the MICs (Minimal Inhibitory Concentration) of twenty isolates, increased the MIC of one isolate, and did net change the MICs of nine isolates. To find out how iron increased susceptibility to ofloxacin, bacterial cells were grown in Muller Hinton (MH) media and succinate minimal media (SMM) to induce iran acquisition systems and the intracellular ofloxacin concentrations were assayed in the presence of iron. The addition of iron to the media decreased the MICs of cells whether they were grown in MH or SMM. Siderophores, carbonyl cyanide m-chlorophenylhydrazone (an inhibiter of proton motive force), and ouabain (an inhibitor of ATPase) did not decrease the effect of iron. Results suggested that the increase in the intracellular ofloxacin concentration by iron is accomplished not by decreasing the efflux but by increasing the of ofloxacin permeability.

• Korean Journal of Veterinary Service
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• v.29 no.1
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• pp.27-36
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• 2006

This studies were carried out to investigate the rearing managements of pig farms and survey on pneumonia of the slaughtered pigs from 5 selected herds located in Jangsu, Jeonbuk. Isolated aerobic microorganisms from pneumonic lung were examined antibiotic susceptibility and tested serological antibody titers of the herd base. Prevalence rate of pneumonia were examined according to rearing and health management conditions of pig farms. Prevalence rate of pneumonia were detected in 78.8% and enzotic pneumonia. In 47.7%, pleuropneurnonia in 31.1%. In serological antibody titers showed the positive reaction with 54.4% in Actinobacillus pleuropneumonia serotype 5, 44.8% in Pasteurella multocida, 36.8% in A pleuropneurnonia serotype 2, 13.6% in Mycoplasmal pneumonia. Isolated aerobic microorganisms were examined antibiotic susceptibility and showed the high activity in gentamicin (58.3%), enrofloxacin (53.3%), norfloxacin (51.6%), cephalothin (41.7%) and low activity in amoxycillin (98.3%), oxytetracycline (98.3%), penicillin G (90.0 %), tetracycline (88.4%), ampicillin (88.3%). Farm managements were deficient effect of humidity in swine house but ammonia gas all appeared the 10 ppm that were recommendation density, below.