• Journal of the Korean Society of Tobacco Science
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• v.27 no.1 s.53
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• pp.11-18
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• 2005

The nicotine converter genotypes of burley tobacco (Nicotiana tabacum L.), which convert nicotine to nornicotine, contain a high amount of nornicotine that degrades tobacco quality and smoking taste. Elimination of nicotine converter plants before seed harvesting is required for breeding nicotine low-converter lines and for increasing their seed production. This study aims to develop a rapid and convenient method of identifying nicotine converter plants of burley breeding lines of KB9118(KB108) using thin-layer chromatography (TLC) and isatin coloration method. Out of 223 plants in 10 lines harvested at maturity in 2002, 102 plants ($45\%$) were identified as nicotine converters by TLC of tobacco leaves air-cured. For 16 lines selected as low-converters in 2002, 148 plants grown in the field in 2003 were tested by the isatin coloration method using two detached leaves at the flowering stage thoroughly sprayed with $1\%\;NaHCO_3$ solution and cured in conditioned chambers for the early identification of nicotine to nornicotine conversion. From these samples, 46 plants ($31\%$) in 4 lines were identified as nicotine converters, indicating that the ratio of converters significantly decreased by one time selection. Mean percent conversion of non-screened lines was $14\%$ higher than that of following generation. Therefore in the burley tobacco, a rapid and convenient means of identifying and removing nornicotine converter plants by the isatin coloration method during growth in the greenhouse or field were effective in reducing the converter plants in the following generation.

• Korean Journal of Plant Taxonomy
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• v.43 no.1
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• pp.56-62
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• 2013

Echinochloa oryzoides (Ard.) Fritsch (Na-do-non-pee), was found at paddy fields of Jeonranamdo on a large scale after a first discovery at Masan, Kyeongsangnamdo. This species was not easily distinguished from rice before flowering at the paddy field because of a similar morphology to rice. It is, however, distinguished from other species of the Korean Echinochloa by reliable morphological characters such as horizontal or drooping panicles at maturity, lower glumes with usually 1/4-2/5 as long as the spikelets, awned lower lemmas, and the number of chromosomes, 2n = 54. Echinochloa oryzicola (Vasinger) Vasinger was distinguished from E. oryzoides by erect panicles, lower glumes with at least 1/2 as long as the spikelets, and the number of chromosomes, 2n = 36.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.10a
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• pp.78-78
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• 2018

패션프루트(Passiflora edulis)는 브라질이 원산지이며 아열대와 열대지역에서 재배되고 있는 다년생 상록덩굴식물로 시계꽃과(Passifloraceae) 시계꽃속(Passiflora)에 속한다. 전 세계적으로 500여종이 분포하고 그 중 10여종은 식용, 나머지는 관상용으로 이용되고 있다. 지구온난화로 인한 기온상승으로 전남지역 재배에 적합한 대체 아열대과수 개발이 필요하다. 패션프루트는 겨울 최저온도가 $3^{\circ}C$이상 조건에서 재배 가능하여 남부 지역는 무가온 하우스재배를 하고 있지만 시설내 6월 하순부터 8월 중순까지 $35^{\circ}C$ 이상 고온이 지속되어 과실을 볼 수 없다. 8월 하순부터 피는 꽃은 인공수분으로 착과되어 11월부터 수확이 가능하지만 이후에 낮은 온도($10^{\circ}C$이하)로 착색이 진행되지 않아서 동해피해로 수확을 포기해야 한다. 어느 시기까지 인공수분을 해야 당년에 안정적인 수확이 가능한지를 알아보고자 패션프루트의 후기(2차) 개화시기 중에서 8월 30일, 9월 10일, 9월 20일, 9월 30일에 인공수분 하여 11월 하순에 착색이 정상적으로 진행되는 처리를 조사한 결과 8월 30일 처리구만 11월 21일 착색이 되었다. 미착색과에서 내용물이 진노랑색으로 진행되면 후숙하여 상품과로 판매가 가능하기 때문에 진노랑색에 도달한 시기를 조사하였다. 조사에서 9월 10일까지 인공수분 처리한 과실은 후숙하여 상품과로 판매가 가능하였다.

• Korean Journal of Agricultural Science
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• v.8 no.1
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• pp.3-10
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• 1981

In order to analyze growth and yield of rice population at the stand point of productive ecology, productive structure and growth rate of rice plants and effects of their related factors were studied. The results obtained were summarized as follows: 1. High crop growth rate was maintained throughout growing season when rice plants grew in population compared to a single rice plant. Since crop growth rate in rice stands were also higher at ripening stage, higher yields were obtained. 2. Higher crop growth rate of rice plants in population was maintained by the increase of leaf area rather than by net assimilation rate. 3. As the overgrowth of upper leaves of plant population in some rice varieties transmission of light into lower leaves was inhibited and thus most of them were eventually died before flowering. 4. In the case of rice population, the ratio of productive organ to non-productive organ, light transmission rate and nitrogen contents of active leaf blade were lower than individual rice plants under isolated condition.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.260-260
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• 2017

High temperature is one of major environmental stress. Heat in flowering season of wheat is able to effect negatively to fertilization and also heat effect to maturity. Therefore, Heat stress effects severely to qualities of wheat and yield productivity. In this study, we evaluated to agronomic characteristics and qualities by high temperature in 13 Korean wheat varieties. Weight of 1,000 grains decreased when wheat got the heat stress. In particular, heat stress during the heading dates were more damaged than after the end of heading dates except two varieties Keumkang and Jokyoung. Plant height of each cultivar under high temperature and normal field averaged 80.5 cm and 83.0cm, respectively. The length of spike and awn in each cultivar were similar to both condition. Flour yield and gluten contents of most heat damaged wheat decreased. Under the temperature, protein contents of six varieties like as Keumkang, Baekjoong, Hojoong, Yeonbaek, Joah and Shinmichal 1 decreased but the others increased. The sedimentation values (SDSF) of four varieties decreased under the high temperature. But SDSF of 7 varieties like as Baekjung, Suan, Hojoong, Jojoong, Uri, Shinmichal and Shinmichal 1 was increased. The lightness (L) of wheat flour derived from high temperature treated wheat was darker than non-treated wheat. As a result of this research, we confirmed that agricultural traits and qualities decreased in heat damaged wheat.

• Journal of Plant Biotechnology
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• v.3 no.2
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• pp.89-94
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• 2001

In vitro plant regeneration of inbred breeding line of hot pepper (Capsicum annuum L.) was established using leaf and petiole segments as explants. About 28 days old plants were excised and cultured on MS medium supplemented with TDZ and NAA or in combination with Zeatin. In all of the media compositions tested, combination of TDZ 0.5 mg/L, Zeatin 0.5 mg/L, and NAA 0.1 mg/L was found to be the best medium for shoot bud initiation. Young petiole was the most appropriate explant type for the plant regeneration as well as genetic transformation in hot pepper. In this study, HpMADS1 gene isolated from hot pepper was introduced using Agrobacterium-mediated transformation system. Based on the analysis of Southern blot and RT-PCR, HpMADS1 gene was integrated in the hot pepper genome. It has been known that floral organ development is controlled by a group of regulatory factors containing the MADS domain. Morphological characteristics in these transgenic plants, especially flowering habit, however, were not significantly altered, indicating this MADS gene, HpMADS1 may be non-functional in this case.

• Journal of Bio-Environment Control
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• v.29 no.4
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• pp.448-455
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• 2020

This study was carried out to investigate reduction of the frost damage using the mesh net used for the purpose of non-bagged cultivation. A device measuring the weight of frost was developed and installed in both the control and the experimental, and the effect of frost reduction was evaluated with their weights. As a result, weight of frost in the control was reduced from 37% to 59% with mesh net on the day the frost was observed. In addition, the device for automatically observing the amount of frost was developed and the height of the windbreak of the frost measuring device was determined to be 30 cm through wind tunnel experiment. The results of this study are expected to reduce frost damage during the flowering season of fruit trees by installing mesh net and it is expected to be used as basic data for agricultural use of mesh net.

• ALGAE
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• v.17 no.1
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• pp.59-68
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• 2002

A phylogenetic affinity between charophytes and embryophytes (land plants) has been explained by a few chloroplast genomic characters including gene and intron (Manhart and Palmer 1990; Baldauf et al. 1990; Lew and Manhart 1993). Here we show that a charophyte, Spirogyra maxima, has the largest operon of angiosperm chloroplast genomes, rpl23 operon (trnⅠ-rpl23-rpl2-rps19-rpl22-rps3-rpl16-rpl14-rps8-infA-rpl36-rps11-rpoA) containing both embryophyte introns, rpl16.i and rpl2.i. The rpl23 gene cluster of Spirogyra contains a distinct eubacterial promoter sequence upstream of rpl23, which is the first gene of the green algal rpl23 gene cluster. This sequence is completely absent in angiosperms but is present in non-flowering plants. The results imply that, in the rpl23 gene cluster, early charophytes had at least two promoters, one upstream of trnⅠ and and another upstream of rpl23, which partially or completely lost its function in land plants. A comparison of gene clusters of prokaryotes, algal chloroplast DNAs and land plant cpDNAs indicated a loss of numerous genes in chlorophyll a+b eukaryotes. A phylogenetic analysis using presence/absence of genes and introns as characters produced trees with a strongly supported clade containing chlorophyll a+b eukaryotes. Spirogyra and embryophytes formed a clade characterized by the loss of rpl5 and rps9 and the gain of trnⅠ (CAU) and introns in rpl2 and rpl16. The analyses support the hypothesis that the rpl23 gene cluster and the rpl2 and rpl16 introns of land plants originated from a common ancestor of Spirogyra and land plants.

• Applied Biological Chemistry
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• v.34 no.2
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• pp.142-148
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• 1991

When cultured soybean immature seed on 15th days after flowering, suitable temperature in formation of callus were $24{\sim}27^{\circ}C$, and embryogenic callus(EC) were generated at medium containing NAA with growth regulators, and then, results were subcultured the EC, a plenty of shoots and roots were formed at medium supplemented BA 2mg/1 and IAA 2mg/1, respectively, however when used at medium the same time supplemented BA 2mg/1 and IAA 2mg/1, formation of cullus was energetic, and a symptom of organization was not showed , Total lipid contents include in each cultures were increased at low temperature of cultural conditions as much as possible, but glycolipid, phospholipid, free sterol contents were a little increased at $24{\sim}27^{\circ}C$, and free sterol content was increased at a case of embryogenic structure were generated. In fatty acid compositions in each cultures, the contents of unsaturated fatty acid were plenty in EC, and unsaturation rate was 0.837. Besides, in sterol compositions, cholesterol content was remarkably high in EC than that of other cultures.

• Journal of Ecology and Environment
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• v.30 no.3
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• pp.271-276
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• 2007

A simple and efficient protocol was developed for culturing Cu-tolerant and Cu-accumulating plants via pre-adaptation to Cu during plant tissue culture. We induced multiple shoots from begonia (Begonia evansiana Andr.) leaf explants on MS medium supplemented with naphtaieneacetic acid and benzyladenine. After 3 months, small plantlets were transferred to MS medium supplemented with $100{\mu}M\;CuCl_2$ for pre-adaptation to Cu and cultured for 5 months. Then, these plantlets were individually planted in pots containing artificial soil. An additional 500 mg of Cu dissolved in 1/4 strength MS solution was applied to each pot during irrigation over the course of 2 months. We planted pre-adapted and control begonias in soil from the II-Kwang Mine, an abandoned Cu mine in Pusan, Korea, to examine their ability to tolerate and accumulate Cu for phytore-mediation. Pre-adapted begonias accumulated $1,200{\mu}g$ Cu/g dry root tissue over the course of 45 days. On the other hand, non-Cu-adapted controls accumulated only $85{\mu}g$ Cu/g dry root tissue. To enhance Cu extraction, chelating agents, ethylenediamine tetraacetic acid (EDTA)-dipotassiun and pyridine-2,6-dicarboxylic acid (PDA), were applied. While the chelating agents did not enhance accumulation of Cu in the roots of control begonias, EDTA application increased the level of Cu in the roots of pre-adapted begonias twofold (to $2,500{\mu}g$ Cu/g dry root tissue). Because pre-adapted begonias accumulated a large amount of Cu, mainly in their roots, they could be used for phytostabilization of Cu-contaminated soils. In addition, as a flowering plant, begonias can be used to create aesthetically pleasing remediation sites.