• Title/Summary/Keyword: non-dig

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Development of dot blot hybridization method using non-radio labeled probes for the diagnosis of malignant catarrhal fever (Dot blot hybridization에 의한 malignant catarrhal fever virus의 진단법 개발)

  • Kim, Ok-Jin
    • Korean Journal of Veterinary Pathology
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    • v.7 no.1
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    • pp.1-4
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    • 2003
  • Malignant catarrhal fever (MCF) is a systemic disease of ruminants caused by a gamma herpesvirus, ovine herpesvirus 2 (OvHV-2). Dot blot hybridization (DBH) protocols for detecting and differentiating this MCF virus were developed. OvHV-2 specific primer pairs, 556/555, were used for the amplification of target DNA. Then, the amplified DNA was labeled with incorporation of digoxigenin (DIG). The Dig-labeled probe was able to detect and differentiate specifically OvHV-2 DNA. This DBH technique can be applied to confirm the presence of MCF virus on clinical samples and to differentiate specifically between OvHV-2 infection and other viral infections.

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A Study on application of Trapezoidal Steel Box Tunnelling Method (지중압입체를 이용한 지하구조물 축조방법의 적용성 연구)

  • Jun, Sung Bai
    • Journal of the Society of Disaster Information
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    • v.4 no.2
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    • pp.138-154
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    • 2008
  • The conventional non-dig underground structure building method which made an appearance to reduce the social and environmental costs and maximize the efficiency of the social overhead capital facilities could not help being uneconomical because of many problems such as unnecessary excessive excavation, water leakage, obstacle interference, difficulty of curvilinear application and connection complexity between propelled and injected bodies due to indiscriminate application of small and large circular steel pipes without consideration of the site conditions. The T.S.T.M, in which a protruded square tube is applied as a propulsion and injection body in a design that considered site conditions such as ground condition, depth of soil and live load, was able to be economical as it solved the problems of water resistance, minimization of obstacle interference and curvilinearity, and we can see that it can be applied to all grounds by utilizing or complementing the target ground in terms of engineering. Also in configuring the transverse section, it is possible to not only secure excellent structural safety but also implement all of the above engineering characteristics not only in the square cross section but also in the arch cross section, so it was possible to build structures on any section or ground, and we could confirm the LCC reduction effect and the VE effect.

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The Effects of ALPHA-NUMERIC Brand Name on the Functional Sportswear, for its Suitability, Brand Personality, and Attitude (기능성 스포츠 의류제품의 알파누메릭(Alpha-Numeric) 브랜드 네임이 제품의 적합성, 브랜드 개성 및 태도에 미치는 영향)

  • Suh, Hyun-Suk;Na, Youn-Kue;Sun, Jin-Young
    • Fashion & Textile Research Journal
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    • v.11 no.4
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    • pp.556-565
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    • 2009
  • This study investigates the effect of Alpha-numeric brand name on the product suitability, brand personality, and attitude. To do that we have chosen the functional sportswear, knowing the fact that the Alpha-numeric brand names are not hard to find in this industry. The changes have been made to Alpha-numeric names, such as the usage/non-usage of number, the size of number being used, the writing method, and the different typeface to suit for the study purpose. The results indicate that the Alpha-numeric brand names do have the effect on increasing the fitness for the product's brand. The bigger number and the cursive script will improve the product's suitability. The customers form positive attitude and favorability on the Alpha-numeric brand name compared to the regular brand name in this industry. The contribution of the study is to dig out the distinction of the communicational effects on the customers when the Alpha-numeric brand names take on the different forms. Therefore the current study enlights the practical value of Alpha-numeric brand name as part of the branding strategy.

Developmental Patterns of mST3GaIV mRNA Expression in the Mouse: In Situ Hybridization using DIG-labeled RNA Probes

  • Ji, Min-Young;Lee, Young-Choon;Do, Su-Il;Nam, Sang-Yun;Jung, Kyu-Yong;Kim, Hyoung-Min;Park, Jong-Kun;Choo, Young-Kug
    • Archives of Pharmacal Research
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    • v.23 no.5
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    • pp.525-530
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    • 2000
  • mST3GaIV synthesizes ganglioside GM3, the precursor for simple and complex a- and b- series gangliosides, and the expression and regulation of mST3GaIV (CMP-NeuAc: lactosylceramide $\alpha$2,3-sialyltransferase) activity is central to the production of almost all gangliosides, a class of glycosphingolipids implicated in variety of cellular processes such as transmembrane signaling, synaptic transmission, specialized membrane domain formation and cell-cell interactions. To understand the developmental expression of mST3GaIV in mice, we investigated the spatial and temporal expression of mST3GaIV mRNA during the mouse embryogenesis [embryonic (E) days; 19, E11, E13, E15] by in situ hybridization with digoxigenin-labeled RNA probes. All tissues from 19 and E11 were positive for mST3GaIV mRNA. On E13, mST3GaIV mRNA was expressed in various neural and non-neural tissues. In contrast to these, on E15, the telencephalon and liver produced a strong expression of mST3GaIV which was a quite similar to that of E13. In this stage, mST3GaIV mRNA was also expressed in some non-neural tissues. These data indicate that mST3GaIV is differently expressed at developmental stages of embryo, and this may be importantly related with regulation of organogenesis in mice.

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Quilitative certificational plan of shanyao (산약(山藥)의 품질인증(品質認證) 방안(方案))

  • Cho, Eun-Hawn;Roh, Seong-Soo;Kil, Ki-Jeong;Seo, Bu-il;Seo, Young-Bae
    • Journal of Haehwa Medicine
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    • v.13 no.2
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    • pp.187-196
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    • 2004
  • Now many sustitution and false articles is used in korea instead of shanyao. To use shanyao correctly, we will make a quilitative certificational plan of shanyao to investigate all of lieraturea, records and documents. And we could reach conclusions as folloews. 1) Source About a source of shanyao, though korea and china has a each other source, we think there is no problem in use of both. 2) Process In our country producing shanyao as medical use is a 'duanma', we can divide into peeling and non-peeling, drying at bulks and at briquets, steaming shanyao and fresh shanyao. Regardless of existence for peeling and steaming, a distributing shanyao is received a proper judgment. Like this result was expressed by insufficiency of standards about medical components or indicating components. We detected a reminding S02 more than 10 ppm. And this expresses that there ia a problum at a drying method. To suggest proper processing methods, a standard of quility will have to be made which the existence of peeling, difference of quility between 'changma' and 'duanma', drying method and exudation test with cutted thickness are adaptable. Besides, 'maoshanyao' and 'guangshanyao' of china is processed by various methods which decrease a medical effect such as too much soaking shanyao in water, steaming with a sulfur, too much peeling, So we must process shanyao like the next methods. (1) When harvesting, dig deeply not to cut off roots. (2) Not to peel, wash shanyao in a washing machine. (3) Dry to 50-60% degree at sunny place or drying machine. (4) To be easy for drying and exudation, cut off a thick piece with 5 mm (5) Dry perfectly at ding machine. 3) Quality3) Quality (1) Functional standards It is not Proper that 'guangshanyao' is used in china because it has a problem with quility on process of working, If they did not soak shanyao in water or heat with steam, it is the real situation that they cannot cutt off shanyao evenly. In conclusion, shanyao must be heavy, powdery with a perfectly non-peeling surface, section surface is yellow-white color, unequal and has no holes. (2) Physicochemical standards It is the real situation that we can not distinguish into quility of shanyao with established test because various workings which decrease medical effects is used. Therefore we suggest a testing standard of S02 which is used in bleaching. And testing standards relatived with decrease of medical effects must be established at once. It must that Dry on loss is less than 14.0%, content of ash is less than 6.0%. Content of acid-nonsoluble ash is less than 0.5%. Contens of heavy metal has to detect less than 30 ppm and there is no reminding agricultural medince.

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A Study on Improved Method of Self-Employment Adequacy Analysis in Korea (한국의 자영업 적정규모 분석방법 개선에 관한 연구)

  • Suh, Geunha;Kim, Sungho;Suh, Changsoo
    • Journal of Distribution Science
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    • v.17 no.3
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    • pp.107-116
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    • 2019
  • Purpose - Why, why is it difficult to predict the appropriateness of self-employment, and what are the countermeasures and policy proposals to overcome. This study intends to further develop the field of statistical variables. It is necessary to overcome the limitation of existing proper scale research in Korea. We need to find statistical variables that can determine the appropriateness of self-employment in Korea. These efforts will be helpful in evaluating OECD countries and statistics and developing domestic economic indicators. Research design, data, and methodology - It is the discovery of statistical indicators and complementary indicators that have not been revealed in previous studies. Therefore, we sought to find new statistical parameters based on the statistics of the Korea National Statistical Office, the Bank of Korea, and overseas OECD statistics. (Proper Size of Adequacy) is defined as the specific gravity or number of the self-employed in Korea, which is shown as "Out Put" by statistical analysis of STATA panel statistical data. It is possible to further develop variables such as gross domestic product, gross national product, economic growth rate, unemployment rate, income tax rate, consumer price, tax level, exports, import amount, bill default I want to dig. Results - In addition to expanding economic indicators that can be explained by self-employment determinants, we have developed a variety of methods such as linear and non-linear (U-shaped, inverted U-shaped). It is the improvement of the self-employment determinants and the analysis method to estimate the appropriate scale. Conclusions - The proposed contents are reflected in self - employment appropriateness evaluation data and hope to help the government to select the policy support and to evaluate the government business after the policy support. These efforts are expected to be of great help to operators operating their own businesses, and to government and related institutional practitioners who support them. In this way, self-employment will be created in accordance with the Korean situation, where the happy life of all the people becomes the premise and the inclusive economic activities are guaranteed. It will improve the method of analyzing proper scale of small business owners and self-employed in Korea.