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A Non-radioisotopic Endpoint Using Bromodeoxyuridine ELISA Method for Murine Local Lymph Node Assay (BrdU ELISA를 이용한 국소 림프절 시험법의 비방사선법 연구)

  • 이종권;박재현;박승희;김형수;정승태;엄준호;윤소미;장은정;최광식
    • Toxicological Research
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    • v.19 no.2
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    • pp.133-139
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    • 2003
  • Allergic contact dermatitis may be caused by a wide variety of chemicals. A murine local lymph node assay (LLNA) has been developed as an alternative to guinea pig models for assessing the contact sensitization potential of chemical. However, there is a need to develop a nonradioisotopic endpoint for the LLNA, because of the radioisotopic method's requiring the use of special facilities. In this study, we investigated the development of a nonradioisotopic endpoint for LLNA using ELISA (enzyme-linked immunosorbent assay). Female Balb/c mice were treated by the topical application on the dorsum of both ears with four different strong sensitizers, 2,4-dinitrochlorobenzene (DNCB), oxazolone (OXZ), toluene diisocyanate (TDI), and trimellitic anhydride (TMA), and a strong irritant, sodium lauryl sulfate (SLS), once daily for three consecutive days. The proliferation of cells in the auricular Iymph node was analyzed by means of the labelling index (Ll) of bromodeoxyuridine (BrdU) incorporation into cells. The weights of the Iymph nodes in the mice treated with allergens, DNCB, OXZ, TDl and TMA were increased compared to the vehicle control. The stimulation index (Sl) of mice treated with DNCB, OXZ, TDl, and TMA was over three-fold increase compared to the vehicle control. However, the S1 of mice exposed to SLS was not significantly increased compared to the vehicle control, while the lymph node weight of SLS was significantly increased. These results suggest that the LLNA modified endpoint using ELISA based on BrdU incorporation could provide a useful method of screening for irritants and allergens.

MLS-Based Finite Elements and a Proposal for Their Applications (MLS기반 유한요소와 그 응용에 관한 제언)

  • Cho, Young-Sam
    • Journal of the Computational Structural Engineering Institute of Korea
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    • v.22 no.4
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    • pp.335-341
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    • 2009
  • In this paper, review of developed MLS-based finite elements and a proposal for their applications are described. The shape functions and their derivatives of MLS-based finite elements are constructed using Moving-Least Square approximation. In MLS-based finite element, using the adequate influence domain of weight function used in MLS approximation, kronecker delta condition could be satisfied at the element boundary. Moreover, because of the characteristics of MLS approximation, we could easily add extra nodes at an arbitrary position in MLS-based finite elements. For these reasons, until now, several variable-node elements(2D variable element for linear case and quadratic case and 3D variable-node elements) and finite crack elements are developed using MLS-based finite elements concept. MLS-based finite elements could be extended to 2D variable-node triangle element, 2D finite crack triangle element, variable-node shell element, finite crack shell element, and 3D polyhedron element. In this paper, we showed the feasibility of 3D polyhedron element at the case of femur meshing.

A Study on Efficient Network Topology Visualization using Node Centrality (노드 중심성을 이용한 효율적 네트워크 토폴로지 시각화 연구)

  • Chang, Beom-Hwan;Ryu, Jemin;Kwon, Koohyung
    • Convergence Security Journal
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    • v.21 no.2
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    • pp.47-56
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    • 2021
  • Network topology visualization has been studied a lot since the past and developed with many tools. The network topology has strength in understanding the overall structure of a network physically and is useful for understanding data flow between nodes logically. Although there are existing tools, not many can be utilized efficiently while using the general network node data structure and express the topology similar to the actual network structure. In this paper, we propose an efficient method to visualize topology using only connection information of network nodes. The method finds the central node by using the centrality, the influence of nodes in the network, and visualizes the topology by dynamically segmenting all nodes and placing network nodes in 3D space using the weight of the child node. It is a straightforward method, yet it effectively visualizes in the form of an actual network structure.

Proposed Message Transit Buffer Management Model for Nodes in Vehicular Delay-Tolerant Network

  • Gballou Yao, Theophile;Kimou Kouadio, Prosper;Tiecoura, Yves;Toure Kidjegbo, Augustin
    • International Journal of Computer Science & Network Security
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    • v.23 no.1
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    • pp.153-163
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    • 2023
  • This study is situated in the context of intelligent transport systems, where in-vehicle devices assist drivers to avoid accidents and therefore improve road safety. The vehicles present in a given area form an ad' hoc network of vehicles called vehicular ad' hoc network. In this type of network, the nodes are mobile vehicles and the messages exchanged are messages to warn about obstacles that may hinder the correct driving. Node mobilities make it impossible for inter-node communication to be end-to-end. Recognizing this characteristic has led to delay-tolerant vehicular networks. Embedded devices have small buffers (memory) to hold messages that a node needs to transmit when no other node is within its visibility range for transmission. The performance of a vehicular delay-tolerant network is closely tied to the successful management of the nodes' transit buffer. In this paper, we propose a message transit buffer management model for nodes in vehicular delay tolerant networks. This model consists in setting up, on the one hand, a policy of dropping messages from the buffer when the buffer is full and must receive a new message. This drop policy is based on the concept of intermediate node to destination, queues and priority class of service. It is also based on the properties of the message (size, weight, number of hops, number of replications, remaining time-to-live, etc.). On the other hand, the model defines the policy for selecting the message to be transmitted. The proposed model was evaluated with the ONE opportunistic network simulator based on a 4000m x 4000m area of downtown Bouaké in Côte d'Ivoire. The map data were imported using the Open Street Map tool. The results obtained show that our model improves the delivery ratio of security alert messages, reduces their delivery delay and network overload compared to the existing model. This improvement in communication within a network of vehicles can contribute to the improvement of road safety.

A Minimum Spanning Tree Algorithm for Directed Graph (방향그래프의 최소신장트리 알고리즘)

  • Choi, Myeong-Bok;Lee, Sang-Un
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • v.11 no.5
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    • pp.159-171
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    • 2011
  • This paper suggests an algorithm that obtains the Minimum Spanning Tree for directed graph (DMST). The existing Chu-Liu/Edmonds DMST algorithm has chances of the algorithm not being able to find DMST or of the sum of ST not being the least. The suggested algorithm is made in such a way that it always finds DMST, rectifying the disadvantage of Chu-Liu/Edmonds DMST algorithm. Firstly, it chooses the Minimum-Weight Arc (MWA) from all the nodes including a root node, and gets rid of the nodes in which cycle occurs after sorting them in an ascending order. In this process, Minimum Spanning Forest (MST) is obtained. If there is only one MSF, DMST is obtained. And if there are more than 2 MSFs, to determine MWA among all MST nodes, it chooses a method of directly calculating the sum of all the weights, and hence simplifies the emendation process for solving a cycle problem of Chu-Liu/Edmonds DMST algorithm. The suggested Sulee DMST algorithm can always obtain DMST that minimizes the weight of the arcs no matter if the root node is set or not, and it is also capable to find the root node of a graph with minimized weight.

HISTOLOGICAL CHANGES OF MOUSE SPLEEN AND LYMPH NODE BY CYCLOPHOSPHAMIDE (Cyclophosphamide에 의(依)한 mouse의 비장(脾臟)과 임파절(淋巴節)의 조직학적(組織學的) 변화(變化))

  • Chung, Hun-Taeg;Ha, Tai-You;Chung, Dong-Kyu
    • The Journal of the Korean Society for Microbiology
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    • v.13 no.1
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    • pp.55-62
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    • 1978
  • Adult mice were injected with a single sublethal dose of cyclophosphamide. Effects of the drug on the body weight, spleen weight, and morphology of the peripheral lymphoid system have been analysed. The body weights of the mice given cyclophosphamide(300mg/kg body weight) decreased slightly and returned to normal quickly. Spleen weights, however, changed greatly by keeping the process of decrease, recovery, overshoot, and gradual return to normal only by 20 days. Histologic examinations of spleen and popliteal lymph node showed that follicles disappeared 1 to 2 days before periarteriolar lymphatic sheath or paracortex. At the peak of splenomegaly, the architectures of spleen and lymph node were replaced with the interstitial tissue composed of dense and uniform layer of lymphoid cells. With the return of spleen weight to normal range, the architecturles returned to normal. Our results clearly indicated that cyclophosphamide affected not only B cells but also T cells. These results seemed to suggest that augmentation of delayed-type hypersensitivity by cyclophosphamide may be due to the eliminateion of the suppressor T cells.

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Game Theoretic MAP Load Balancing Scheme in HMIPv6 (HMIPv6에서 게임 이론을 이용한 MAP 부하 분산 기법)

  • Ki, Bum-Do;Kim, Sung-Wook
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.35 no.7B
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    • pp.991-1000
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    • 2010
  • The Hierarchical Mobile IPv6 (HMIPv6) has been proposed to accommodate frequent mobility of the Mobile Node. HMIPv6 can effectively reduce the signaling overhead and latency. However, it has a problem that the registration of a mobile node concentrates on the furthest MAP(Mobility Anchor Point) when the mobile node enters into a new domain. This paper proposes a new load distribution mechanism by using the concept of Nash Bargaining Solution. The main advantage of the proposed scheme can prevent load concentration from being registered to the specified MAP based on the weight value according to the available resource-ratio of a MAP. With a simulation study, the proposed scheme can improve network performance under widely diverse traffic load intensities.

Efficient Energy and Position Aware Routing Protocol for Wireless Sensor Networks

  • Shivalingagowda, Chaya;Jayasree, P.V.Y;Sah, Dinesh.K.
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.14 no.5
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    • pp.1929-1950
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    • 2020
  • Reliable and secure data transmission in the application environment assisted by the wireless sensor network is one of the major challenges. Problem like blind forwarding and data inaccessibility affect the efficiency of overall infrastructure performance. This paper proposes routing protocol for forwarding and error recovery during packet loss. The same is achieved by energy and hops distance-based formulation of the routing mechanism. The reachability of the intermediate node to the source node is the major factor that helps in improving the lifetime of the network. On the other hand, intelligent hop selection increases the reliability over continuous data transmission. The number of hop count is factor of hop weight and available energy of the node. The comparison over the previous state of the art using QualNet-7.4 network simulator shows the effectiveness of proposed work in terms of overall energy conservation of network and reliable data delivery. The simulation results also show the elimination of blind forwarding and data inaccessibility.

Using Lymphovenous Anastomosis and Lymph Node to Vein Anastomosis for Treatment of Posttraumatic Chylothorax with Increased Thoracic Duct Pressure in 3-Year-Old Child

  • Kim, Yeongsong;Kim, Hyung B.;Pak, Changsik J.;Suh, Hyunsuk P.;Hong, Joon P.
    • Archives of Plastic Surgery
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    • v.49 no.4
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    • pp.549-553
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    • 2022
  • Chylothorax is a rare disease and massive lymph fluid loss can cause life-threatening condition such as severe malnutrition, weight loss, and impaired immune system. If untreated, mortality rate of chylothorax can be up to 50%. This is a case report of a 3-year-old child with iatrogenic chylothorax. Despite conservative treatment and procedures, like perm catheter insertion, the patient failed to improve the respiratory symptoms over 3 months of period. As an alternative to surgical option, such as pleurodesis and thoracic duct ligation which has high complication rate, the patient underwent lymphovenous anastomosis (LVA) and lymph node to vein anastomosis (LNVA). Follow-up at fourth month showed clear lungs without breathing difficulty despite perm catheter removal. This is the first report to show the effectiveness of LVA and LNVA against iatrogenic chylothorax.

Studies on the Fruiting Phase of Rape Under the Different Cultural Conditions

  • Kae, B.M.
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.12
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    • pp.77-87
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    • 1972
  • The fruiting phase of rape under transplanting and direct-sowing conditions has been studied at Mokpo during the 2 years period from 1970 to 1971. Two varieties, Yudal and Miyuki were used in this study. The planting space and sowing time were also incorporated into this study. The results could be summarized as follows: 1. The plant tape of rape was nearly umbrella-shaped of all, but has changed to the laid elliptical-shaped, broadly ovate and spindle-shaped under different varieties and cultural conditions in the plant diagram(Fig. 2). 2. The length of the primary branches for each nodes had a tendency to the symmetric apical curve with the apex at the upper 10-12th node in the transplanting. but to the upper bias apical curve with the apex at the upper 5-7th node in the dense-sowing(Fig.3). 3. The ear of main stem was longer, more pods, heavier 1, 000 grains and more grain yield than ear of primary branches of all, Especially, as for that, the rate of yield constitution per plant in the direct-sowing was higher than in the transplanting(Fig. 4, 5, 6, 7, 8, 9). 4. The ear-length of the primary branches for each nodes had a tendency to the relatively slowly apical curve with the apex at the upper 3-4th node in the transplanting, but to the lower bias apical curve with the apex at the upper 2nd node in the dense-sowing. Especially, the possibility of growth at the lower ears was few in the early variety (Fig. 4). 5. The number of pod per ear on the primary branches for each nodes had a .tendency to the curve of ear-length with the apex at the upper 5-8thnode in the transplanting and at the upper 4-5th node in the dense-sowing (Fig. 5). Accordingly, a high positive correlation was found between the ear-length and number of pod per ear (Table 2) 6. In the transplanting, the high rate of effective ear was from the upper nods to the 12th node, but below the 16-17th nodes was ineffective. However, in the early dense-sowing the high rate of effective was to the 7th node. but below the 10th nodes was. ineffective. Especially, in the early variety has difficult to secure of poi-numbers for ineffective of the lower nodes(Fig. 6.). 7. The density of pod setting of the ear of main stem was the longest of all ears, and the lower nods were, the shorter it became. That had a tendency to the evidently apical growth. However. in the early variety, it was lengthened according to growth of ear-length(Fig.7). 8. The pod-length of the medium nodes was longer than the upper and lower, and the possitive correlation between pod-length and number of grain per poi was very high(Table 2.). 9. In the grain yield per node of primary branches, the most yielding node of transplanting was the upper 9th node, of dense-sowing 4-5th node(Fig 8.), and the possitive correlation between grain yield per node and ear-length or number of pod per ear was very high(Table 2). 10. The grain yield of ear of main stem was higher than that of primary branches in the percentage of dependence for grain yield per plant. The limint node of 50% of dependence to cumulative grain yield per plant was the upper 7-8th node in tranplanting, in the early dense-sowing 4-5th node, and in tke late dense-sowing-3th node(Fig. 9). 11. In the weight of 1, 000 grains the lower nodes were, the lighter it becames in dense-sowing. Therefore, this was also lighter than in the transplanting to the (Fig. 10.). 12. The oil content of grain at the medium nodes was low in the early variety, but at the ear of main stem and upper 1st node it was extremely high(Fig. 11.).

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