• The Journal of the Convergence on Culture Technology
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• v.4 no.4
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• pp.367-372
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• 2018

This paper proposed an algorithm that detects boundary nodes effectively in wireless sensor network. A boundary node is a sensor that lies on the border of network holes or the outer boundary of wireless sensor network. Proposed algorithm detects boundary nodes using only the position information of sensors. In addition, to improve detect performance, sensor computes the overlap area of nearest sensor first. Simulation is performed to validate the process of the proposed algorithm. In Simulation, several obstacles are placed and varying number of sensors in the range of 500~1500 are deployed in the area in order to reflect real world. The simulation results shows that proposed algorithm detects boundary nodes effectively that are located on the border of holes and the outer boundary of wireless sensor network.

• Journal of Plant Biotechnology
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• v.44 no.2
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• pp.171-177
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• 2017

A callus-mediated regeneration protocol for sea-milkwort, an endangered coastal plant species in South Korea, is reported here. The explants of in vitro-plantlets generated from a node culture revealed distinguishable responses in callus induction depending on genotype, explant source, light condition, and 2,4-D concentration. Especially, continuous darkness exclusively facilitated callus induction from explants prior to other treatments. The calli initiated on the media with 2,4-D ranging from 0.1 mg/L to 3.0 mg/L in the dark vigorously proliferated when subcultured on the same media in continuous darkness. Given 1.0 mg/L zeatin in addition to darkness to the calli of the 'Pistachio' genotype, normal adventitious shoots were only regenerated from nodular structures that formed earlier from the calli at the frequency of 24.4 percent. Regenerated shoots easily grew into plantlets with roots and green color on a phytohormone-free MS medium under lighted condition, that were used for node culture as plant materials. Node culture effectively multiplied plantlets in accordance with protocol by Bae et al. (2016). Acclimatized plantlet clusters developed mature plant clusters under inland environment, followed by flowering the following April. Results were merged with node culture protocol suggested by Bae et al. (2016), which, as an in vitro propagation system for sea-milkwort, may contribute to natural habitat restoration.

• Journal of the Korea Society of Computer and Information
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• v.24 no.4
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• pp.169-176
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• 2019

Wireless sensor networks are used to monitor and control areas in a variety of military and civilian areas such as battlefield surveillance, intrusion detection, disaster recovery, biological detection, and environmental monitoring. Since the sensor nodes are randomly placed in the area of interest, separation of the sensor network area may occur due to environmental obstacles or a sensor may not exist in some areas. Also, in the situation where the sensor node is placed in a non-relocatable place, some node may exhaust energy or physical hole of the sensor node may cause coverage hole. Coverage holes can affect the performance of the entire sensor network, such as reducing data reliability, changing network topologies, disconnecting data links, and degrading transmission load. It is possible to solve the problem that occurs in the coverage hole by finding a coverage hole in the sensor network and further arranging a new sensor node in the detected coverage hole. The existing coverage hole detection technique is based on the location of the sensor node, but it is inefficient to mount the GPS on the sensor node having limited resources, and performing other location information processing causes a lot of message transmission overhead. In this paper, we propose an Adjacent Matrix-based Hole Coverage Discovery(AMHCD) scheme based on connectivity of neighboring nodes. The method searches for whether the connectivity of the neighboring nodes constitutes a closed shape based on the adjacent matrix, and determines whether the node is an internal node or a boundary node. Therefore, the message overhead for the location information strokes does not occur and can be applied irrespective of the position information error.

• Journal of Plant Biotechnology
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• v.33 no.4
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• pp.263-270
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• 2006

Pongamia pinnata Pierre is a tree legume, having potential in production of raw material for biodiesel. A protocol for in wk propagation of this plant was standardized using seedling explants. Growth regulators (GR) including gibberellic acid $(GA_3),\;N^6-benzylaminopurine(BA)$, thidiazuron (TDZ), and Adenine sulphate (Ads) were tested for optimum germination of seeds. Removal of seed coat prior to germination, controlled fungal growth partially but enhanced bacterial growth. Antibiotic cefotaxime was ineffective in controlling bacterial contamination. Seedling derived nodal explants and cotyledon nodes with attached cotyledons were excised and cultured for induction of shoots. Optimum sprouting and multiplication of shoot buds were obtained in MS medium supplemented with $8.88{\mu}M$ BA. These buds differentiated and rooted on medium devoid of GR. Optimum growth of Pongamia seedling was obtained in cotton plugged culture vessels. Reculturing of the cotyledon node explants produced more shoots from the same site. This process of removing shoots and reculturing of cotyledon node was followed for eight passages yielding 4 to 8 shoots in each cycle. The shoots (75%) rooted on half strength MS basal medium supplemented with 0.22% charcoal. All plants survived on transfer to soil. This is the first report on in vitro regeneration of Pongamia pinnata. This report demonstrates the possibility of coupling more than one parameter in single experiment to hasten the process of standardization. The process of cycling the nodal explant repeatedly for production of large number of shoots from single meristem may find application in genetic transformation experiments wherein meristems are used for transformation.

• Journal of Korea Multimedia Society
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• v.20 no.4
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• pp.640-648
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• 2017

Sensors have limited resources in sensor networks, so efficient use of energy is important. In order to use the energy of the sensor node efficiently, researches applying mobile sink to the sensor network have been actively carried out. The sink node moves the sensor network, collects data from each sensor node, which spread the energy concentrated around the sink node, thereby extending the entire life cycle of the network. But, when the sink node moves, it requires a reset of the data transmission path, which causes a lot of control messages and delays. In this paper, we propose a CMS(Cluster-based Mobile Sink) method to support the movement of mobile sink in a cluster sensor environment. The proposed scheme minimizes an amount of control messages without resetting the routing paths of entire sensor networks by supporting the sink mobility path using the neighbor cluster list. And, it simplifies the routing path setup process by setting a single hop path between clusters without a gateway. The experiment results show that the proposed scheme has superior energy efficiency in processing and network structure, compared with existing clustering and mesh routing protocols.

• Journal of Korea Multimedia Society
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• v.24 no.3
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• pp.431-439
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• 2021

Since the sensor nodes are randomly arranged in the region of interest, it may happen that the sensor network area is separated or there is no sensor node in some area. In addition, after the sensor nodes are deployed in the sensor network, a coverage hole may occur due to the exhaustion of energy or physical destruction of the sensor nodes. The coverage hole can greatly affect the overall performance of the sensor network, such as reducing the data reliability of the sensor network, changing the network topology, disconnecting the data link, and worsening the transmission load. Therefore, sensor network coverage hole recovery has been studied. Existing coverage hole recovery studies present very complex geometric methods and procedures in the two-step process of finding a coverage hole and recovering a coverage hole. This study proposes a method for discovering and recovering a coverage hole in a sensor network, discovering that the sensor node is a boundary node by itself, and determining the location of a mobile node to be added. The proposed method is expected to have better efficiency in terms of complexity and message transmission compared to previous methods.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.49 no.1
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• pp.69-72
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• 2004

Somaclonal variation, defined as phenotypic and genetic variations among regenerated plants from a parental plant, could be caused by changes in chromosome structure, single gene mutation, cytoplasm genetic mutation, insertion of transposable elements, and DNA methylation during plant regeneration. The objective of this study was to evaluate DNA variations among somaclonal variants from the cotyledonary node culture in soybean. A total of 61 soybean somaclones including seven $\textrm{R}_1$ lines and seven $\textrm{R}_2$ lines from Iksannamulkong as well as 27 $\textrm{R}_1$ lines and 20 $\textrm{R}_2$ lines from Jinju 1 were regenerated by organogenesis from the soybean cotyledonary node culture system. Field evaluation revealed no phenotypic difference in major agronomic traits between somaclonal variants and their wild types. AFLP and SSR analyses were performed to detect variations at the DNA level among somaclonal variants of two varieties. Based on AFLP analysis using 36 primer sets, 17 of 892 bands were polymorphic between Iksannamulkong and its somaclonal variants and 11 of 887 bands were polymorphic between Jinju 1 and its somaclonal variants, indicating the presence of DNA sequence change during plant regeneration. Using 36 SSR markers, two polymorphic SSR markers were detected between Iksannamulkong and its somaclonal variants. Sequence comparison amplified with the primers flanking Satt545 showed four additional stretches of ATT repeat in the variant. This suggests that variation at the DNA level between somaclonal variants and their wild types could provide basis for inducing mutation via plant regeneration and broadening crop genetic diversity.

• Journal of Plant Biotechnology
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• v.34 no.2
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• pp.161-166
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• 2007

In vitro shoot regeneration and multiple shoot induction has been obtained from the stolen node explants in Eremochloa ophiuroides (Munro) Hack. The highest number of shoots ($10.66{\pm}0.21$) was observed from initial explants after one month culture duration on Murashige and Skoog (MS) medium containing 6-benzyladenine (BA: 0.5 mg/l). First generation shoot was excised and sub-cultured on the same fresh media for further multiplication of shoots. An enhanced number of second round shoots ($15.33{\pm}0.21$) was obtained compared to the initial culture media containing BA (0.5 mg/l). The number of shoots/stolon node was higher among all the concentrations of BA than kinetin (KN). In vitro regenerated shoots were successfully rooted in the phytohormone free MS medium. Plantlets generated with roots were transferred to pots containing compound mixture of soil and kept in green house conditions. Acclimatized plants showed 100% survival rate with normal morphology in green house conditions. The present study demonstrates the effect of explant and different plant growth regulators towards in vitro response in E. ophiuroides. Moreover, the study reveals the effect of cytokinin on induction of shoot number per stolen node explant in E. ophiuroides.

• The Journal of the Convergence on Culture Technology
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• v.8 no.2
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• pp.379-384
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• 2022

In a wireless sensor network, a large number of sensor nodes are deployed in an environment where direct access is difficult. It is difficult to supply power, such as replacing the battery or recharging it. It is very important to use the energy with the sensor node. Therefore, an important consideration to increase the lifetime of the network is to minimize the energy consumption of each sensor node. If the energy of the wireless sensor node is exhausted and discharged, it cannot function as a sensor node. Therefore, it is a method proposed in various protocols to minimize the energy consumption of nodes and maintain the network for a long time. We consider the center point and residual energy of the cluster, and the plot point and K-means (WSN suggests optimal clustering). We want to evaluate the performance of the KOCED protocol. We compare protocols to which the K-means algorithm, one of the latest machine learning methods, is applied, and present performance evaluation factors.

• The Journal of the Convergence on Culture Technology
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• v.8 no.6
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• pp.531-536
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• 2022

Megacities are emerging all over the world and gradually becoming the center of an country. Therefore, Megacities are inevitably exposed to various threats such as existing, potential, supra-national, and non-military. In particular, as seen in the recent Ukraine-Russia war, national infrastructure systems has become a major target of enemies. However, considering the size of Megacities, various types of threats, and limited resources, it is impossible to completely protect all national infrastructure systems. Therefore, wisdom is necessary to identify and protect critical nodes. The critical node is the vital point to which the national infrastructure is connected. To this end, in this study, as a method for logically and systematically identifying critical nodes, Node centrality analysis method, which is a network theory, and the Center of Gravity analysis method, which is a military theory, were presented.