• Journal of Korean Society of Forest Science
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• v.111 no.1
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• pp.108-114
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• 2022

In bamboo, the nutrient status of tissues and associated soil is an important indicator of nutrient uptake by various bamboo species. In this study, the nutrient concentrations of leaves, roots, and mineral soil at 0-10 cm depths were examined in three bamboo stands [Phyllostachys bambusoides S ieb. et Zucc, Phyllostachys nigra var. henonis Stapf ex. Rendle, and Phyllostachys pubescens (Mazel) Ohwi] at a broad regional scale in southern Korea. In the three bamboo species, species-specific differences were observed in the carbon (C) and calcium (Ca) concentrations of leaves and in the nitrogen (N) and magnesium (Mg) concentrations of roots. Ca concentrations in leaves were significantly higher in P. bambusoides (11.94 g Ca kg^-1) than in P. pubescens (7.83 g Ca kg^-1), whereas potassium (K) concentrations were lowest in P. bambusoides among the three bamboo species. N concentrations in the roots were significantly lower in P. pubescens (5.23 g N kg^-1) than in P. nigra var. henonis (7.72 g N kg^-1). In contrast to bamboo tissues, soil nutrients, such as total N, organic C, exchangeable Ca²⁺, and Mg²⁺, did not differ significantly among the bamboo species. These results suggest that species-specific practices will be required for nutrient management of bamboo stands because nutrient concentrations vary considerably in the tissues of the three studied species.

• Microbiology and Biotechnology Letters
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• v.51 no.1
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• pp.37-42
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• 2023

Neutral and non-essential amino acid, glutamine (Gln), plays an essential role in supplying nitrogen to all the amino acids and nucleotides in the mammalian body. Gln is also the most important carbon source that provides intermediates for gluconeogenesis and fatty acid synthesis and supplements the tricarboxylic acid cycle in fast-growing cancer cells. Among the known 14 Gln transporter genes, soluted carrier family 1 member 5 (SLC1A5) has been reported to be closely associated with cancer cell growth. Three variants (v1, v2, and v3) have been derived from SLC1A5. Here, we established a heterologous gene expression system for the active form of human SLC1A5 variant-2 (hSLC1A5v2) in Escherichia coli. v2 is the smallest variant that has not yet been studied. Four expression systems were investigated: pBAD, pCold, pET, and pQE. We also addressed the problem of codon usage bias. Although pCold and pET overexpressed hSLC1A5v2 in E. coli, they were functionally inactive. hSLC1A5v2 using the pBAD system was able to catalyze the successful transport of Gln, even if it was not highly expressed. Initial activity of hSLC1A5v2 for [¹⁴C] Gln uptake in E. coli reached up to 6.73 μmole·min^-1·gDW^-1 when the cell was induced with 80 mM L-arabinose. In this study, we demonstrated a heterologous expression system for the human membrane protein, SLC1A5, in E. coli. Our results can be used for the functional comparison of SLC1A5 variants (v1, v2, and v3) in future studies, to facilitae the developement of SLC1A5 inhibitors as effective anticancer drugs.

• Journal of Ginseng Research
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• v.47 no.3
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• pp.469-478
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• 2023

Background: Nitrogen (N) is an essential macronutrient for plant growth and development. To support agricultural production and enhance crop yield, two major N sources, nitrate and ammonium, are applied as fertilizers to the soil. Although many studies have been conducted on N uptake and signal transduction, the molecular genetic mechanisms of N-mediated physiological roles, such as the secondary growth of storage roots, remain largely unknown. Methods: One-year-old P. ginseng seedlings treated with KNO₃ were analyzed for the secondary growth of storage roots. The histological paraffin sections were subjected to bright and polarized light microscopic analysis. Genome-wide RNA-seq and network analysis were carried out to dissect the molecular mechanism of nitrate-mediated promotion of ginseng storage root thickening. Results: Here, we report the positive effects of nitrate on storage root secondary growth in Panax ginseng. Exogenous nitrate supply to ginseng seedlings significantly increased the root secondary growth. Histological analysis indicated that the enhancement of root secondary growth could be attributed to the increase in cambium stem cell activity and the subsequent differentiation of cambium-derived storage parenchymal cells. RNA-seq and gene set enrichment analysis (GSEA) revealed that the formation of a transcriptional network comprising auxin, brassinosteroid (BR)-, ethylene-, and jasmonic acid (JA)-related genes mainly contributed to the secondary growth of ginseng storage roots. In addition, increased proliferation of cambium stem cells by a N-rich source inhibited the accumulation of starch granules in storage parenchymal cells. Conclusion: Thus, through the integration of bioinformatic and histological tissue analyses, we demonstrate that nitrate assimilation and signaling pathways are integrated into key biological processes that promote the secondary growth of P. ginseng storage roots.

• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.133-133
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• 2022

Temperature is a crucial environmental factor for rice cultivation due to the climate change and can influence the rice growth and development. Therefore, the effect of temperature on plant growth characters was examined during the vegetative stage. Plants were grown under three different temperatures: 23℃/13℃ for 18℃, 26℃/16℃ for 21℃ and 29℃/19℃ for 24℃ in the phytotron. The temperature was treated after transplanting and ended in early panicle initiation stage. Heading date of the two varieties were strongly affected by the temperature and were delayed in the 18℃. The plant height in the 18℃ was 21 % shorter than the 21℃ and 24℃ and the tiller and leaf number were increased in the 18℃. All the growth rates of the characters were the slowest in 18℃. The stem dry weight was significantly increased in 18℃. Nitrogen content was increased in the leaves of 18℃ whereas available phosphate and potassium content was found to be increased in the stems of 21℃ and 24℃. OsNRT 2.1 was overexpressed in the leaves and stems of 18℃ and OsNRT2.3a could be expressed in 18℃ and 21℃ temperatures whereas more expressed in 21℃. OsPT1 and OsPT6 could be expressed in the leaf of 18℃ and 24℃ but could be expressed in the stem of 18℃. OsHAK1 and OsHAK5 could be overexpressed in the leaves and stems of 18℃. For hormone, OsCKX2 gene was found to be up regulated in the leaves of 18℃ and OsIAA1 gene could be expressed more in the stem of 24℃.

• Korean Journal of Agricultural Science
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• v.49 no.4
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• pp.697-706
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• 2022

The effect of varied light intensities on the growth of daughter plants during propagation and after transplant to raised beds were examined in 'Sulhyang' strawberry. To this end, four treatments in controlling solar radiation inside a plastic house were made: 55% retractable shading and 35, 55, and 75% fixed shading. The plastic house was shaded only from 11:00 to 16:00 in June and 10:00 to 16:00 in July to September for the treatment of 55% retractable shading. The mean solar radiation inside the plastic house in the retractable 55% shading treatment was 317 W·m^-2 and those in the 35, 55, and 75% fixed shading treatments were 183, 165, and 116 W·m^-2, respectively, at 10 o'clock in the morning. The 55% and 75% fixed shading resulted in taller daughter plants with wider leaf areas than 55% retractable shading. The retractable shading also showed higher leaf numbers, crown diameters, root weights, and fresh weights compared to fixed shading treatments. Regarding the inorganic element contents, daughter plants grown under 75% fixed shading had 1.35% total nitrogen content followed by 1.19% in 35% fixed shading, 1.14% in 55% fixed shading, 1.14% in open culture, and 1.10% in 55% fixed shading. After 54 days following the transplant of daughter plants to a raised bed, the fresh weight of the aboveground part was the heaviest in the 55% retractable shading and non-shading treatments. The 75% fixed shading treatment had the lowest fresh weight of the aboveground plant parts. The results of this study could be used for the production of high-quality daughter strawberry plants.

• Korean Journal of Soil Science and Fertilizer
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• v.2 no.1
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• pp.53-68
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• 1969

The nutriophysiological response of rice plant to root environment was investigated with eye observation of root development and rhizosphere in situation. The results may be summarized as follows: 1) The quick decomposition of organic matter, added in low yield soil, caused that the origainal organic matter content was reached very quickly, in spite of it low value. In high yield soil the reverse was seen. 2) In low yield soil root development, root activity and T/R value were very low, whereas addition of organic matter lowered them still wore. This might be contributed to gas bubbles around the root by the decomposition of organic matter. 3) Varietal difference in the response to root environment was clear. Suwon 82 was more susceptible to growth-inhibitine conditions on low-yield soil than Norin 25. 4) Potassium uptake was mostly hindered by organic matter, while some factors in soil hindered mostly posphorus uptake. When the organic matter was added to such soil, the effect of them resulted in multiple interaction. 5) The root activity showed a correlation coeffieient of 0.839, 0.834 and 0.948 at 1% level with the number of root, yield of aerial part and root yield, respectively. At 5% level the root-activity showed correlation-coefficient of 0.751, 0.670 and 0.769 with the uptake of the aerial part of respectively. N, P and K and a correlation-coefficient of 0.729, 0.742 and 0.815 with the uptake of the root of respectively N.P. and K. So especially for K-uptake a high correlation with the root-activity was found. 6) The nitrogen content of the roots in low-yield soil was higher than in high-yield soil, while the content in the upper part showed the reverse. It may suggest ammonium toxicity in the root. In low-yield soil Potassium and Phosphorus content was low in both the root and aerial part, and in the latter particularly in the culm and leaf sheath. 7) The content of reducing sugar, non-recuding sugar, starh and eugar, total carbohydrates in the aerial part of plants in low yield soil was higher than in high yield soil. The content of them, especially of reducing sugar in the roots was lower. It may be caused by abnormal metabolic consumption of sugar in the root. 8) Sulfur content was very high in the aerial part, especially in leaf blade of plants on low yield soil and $P_2O_5/S$ value of the leaf blade was one fifth of that in high yield soil. It suggests a possible toxic effect of sulfate ion on photophosphorization. 9) The high value of $Fe/P_2O_5$ of the aerial part of plants in low yield soil suggests the possible formation of solid $Fe/PO_4$ as a mechanical hindrance for the translocation of nutrients. 10) Translocation of nutrients in the plant was very poor and most nutrients were accumulated in the root in low yield soil. That might contributed to the lack of energy sources and mechanical hindrance. 11) The amount of roots in high yield soil, was greater than that in low yield soil. The in high-yield soil was deep, distribution of the roots whereas in the low-yield soil the root-distribution was mainly in the top-layer. Without application of Nitrogen fertilizer the roots were mainly distributed in the upper 7cm. of topsoil. With 120 kg N/ha. root were more concentrated in the layer between 7cm. and 14cm. depth. The amount of roots increased with the amount of fertilizer applied.

• Korean Journal of Soil Science and Fertilizer
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• v.45 no.6
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• pp.992-997
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• 2012

This study was conducted to get the basic information for absorb enhancement of accumulated soil nutrients in plastic film house. The grain sorghum (Sorghum bicolor L.) was sowing in plastic film house which soil nutrient accumulated moderately and was cutting at major growth period of sorghum. We were analyzed the regrowth pattern, biomass due to cutting time and amount of plant nutrient of grain sorghum. The obtained results were as follows. The heading date after cutting of sorghum in plastic film house was came to about 35 days. The accumulated of plant height were the longest as 379.4 cm in cutting at milk stage. The total biomass of sorghum in cutting at heading stage was 1.73 ton $10a^{-1}$ in cutting at heading stage. The high grain yields were produced with non-cutting and cutting at 10 leaves stage as 75~113 kg $10a^{-1}$ but the lowest grain yields were the cutting plots at booting stage as below 24 kg $10a^{-1}$. The content of nutrient in sorghum plant was low as progress of growth. The concentrations in aboveground sorghum due to plant parts was in order to leaves > panicle > stalk. The nitrogen content of sorghum was 0.6~0.7% in stalk, 1.5~1.6% in panicle and 1.8~2.3% in leaves. The amount of nutrient absorbed in sorghum was 4.2 kg $10a^{-1}$ in nitrogen, 1.7 kg $10a^{-1}$ phosphorus and 7.7 kg $10a^{-1}$ in potassium and the absorbing different by cutting time in order to booting > non-cutting > panicle formation ${\geq}$ milk ripe > 10 leaves stage.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.39 no.6
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• pp.548-555
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• 1994

To compare the tolerance of crops to acid rain at different growth stages a simulated acid rain (SAR) of pH 2.7 was applied to rice, soybean, and hot pepper from vegetative growth stage to harvest (Veget.-Harvest) and from reproductive growth stage to harvest (Reprod.-Harvest). Visual damages of crops by SAR were greater in the order of rice < hot pepper < soybean and greater at Veget.-Harvest than at Reprod.-Harvest treatment. Chlorophyll content of all crops was greater in the order of Veget.-Harvest < Reprod.-Harvest treatment < control, but photosynthetic activity was not affected by SAR treatments. Nitrogen concentration and uptake of rice plants at harvest were similar among SAR treatments, but those of soybean and hot pepper were greater at Veget.-Harvest treatment than at Reprod.-Harvest treatment or control. Sulfur concentration of all crops was not affected by SAR treatments, but total sulfur uptake of soybean was greater in SAR treatments than untreated control. Grain yield of rice and soybean was not affected by SAR although grain fertility, percent ripened grains, and 1,000-grain weight of rice at Veget.-Harvest treatment were lower compared with Reprod.-Harvest treatment or control. Fruit dry weight of hot pepper was greater in the order of Veget.-Harvest < Reprod.-Harvest < control due to decreased fruit number per plant and average fruit weight. At one time application of SAR at flowering stage, brown spots were observed on the spikelets of rice at below pH 2.3. Petals of soybean and hot pepper were wilted at pH below 1. 7 and 2.0, respectively, but fruit setting was not affected by the pH of the SRA.

• Korean Journal of Soil Science and Fertilizer
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• v.45 no.5
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• pp.749-759
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• 2012

A field experiment was conducted to selection of ground-based remote sensor and reflectance indices to estimate rice production, estimation of suitable season for ground-based remote sensor and N top dressing fertilizer application rate in 2010. Fertilizer application was determined by "Fertilizer management standard for crops" (National Academy of Agricultural Science, 2006). Four levels of N-fertilizer were applied as 0%, 70%, 100% and 130% by base N-fertilizer application and were fertilized as 70% of basal dressing and 30% as top dressing. Rice (Oryza sativa L.) of Chucheong and Joonam (Korean cultivar) were planted on May 22, 2010 in sandy loam soil and harvested on October 6, 2010. Reflectance indices were measured 7 times from July 5 to August 23 by Crop circle-amber and red version and GreenSeeker-green and red version. Remote sensing angle from the sensor head to the canopy of rice was adjusted to $45^{\circ}$, $70^{\circ}$ and $90^{\circ}$ degree because of difference in the density of plant and the sensing angle. The reflectance indices obtained ground-based remote sensor were correlated with the biomass of rice at the early growth stage and at the harvest with $70^{\circ}$ and $90^{\circ}$ degree of sensor angle. The reflectance indices at the 52th Day After Transplanting (DAT) and the 59th DAT, critical season, were positively correlated with dry weight and nitrogen uptake. Specially NDVI at the 59th was significantly correlated with the mentioned parameters. Based on the result of this study, rNDVI by GreenSeeker on $70^{\circ}$ degree of angle at the 59th DAT in Chucheong and rNDVI by Crop Circle on $70^{\circ}$ degree of angle and gNDVI by GreenSeeker on $70^{\circ}$ degree of angle at the 59th DAT in Joonam can be useful for estimation of dry weight and nitrogen uptake. Moreover, sufficiency index estimated by reflectance index at the 59th DAT can be useful for the estimation of N-fertilizer level application and can be used as a model for N-top dressing fertilizer management.

• Korean Journal of Soil Science and Fertilizer
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• v.18 no.3
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• pp.312-317
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• 1985

Growth response and nutrients uptake of tobacco grown on mulched and non mulched plots were investigated during the growing period. 1. The whole growing time was about 25 days shorter and relative growth rate was faster in the mulched plot than in the non mulched plot. 2. Nitrate content of leaf in the latter stage of tobacco grown on the non mulched plot was more continuously increased compared with mulched plot. As a result in the latter stage of tobacco leaf nitrate form of nitrogen level was much higher on non mulched plot than on mulched plot. 3. Reducing sugar content in cured leaves of tobacco grown on the non mulched plot was lower that of the mulched plot. This difference in reducing sugar content was remarkable in the bottom leaves that nitrate content was highest. 4. Tobacco growing on nonmulched soil may cause to be low quality owing to increasing of nitrogen compound and decreasing of reducing sugar content in cured leaves. 5. As distributions of nitrate and potassium content in leaves at different stalk position was similar each other, nitrate and potassium ion considered to be counter ion in absorption and translocation through the tobacco plant.