• Food Science of Animal Resources
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• v.42 no.4
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• pp.712-722
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• 2022

In this study, we investigated the residual properties of tebuconazole-treated pigs. Twenty pigs were treated with different concentrations (0.25, 1.25, 2.5, 12.5, and 25 mg/kg bw/d) of tebuconazole for 28 d. Blood biochemistry, histology, and residual levels were analyzed using the VetTest analyzer, Masson's trichrome staining kit, and liquid chromatography-mass spectrometry, respectively. The final body weights were not significantly different between the control and treatment groups. Alkaline phosphatase, blood urea nitrogen, cholesterol, and gamma-glutamyl transpeptidase levels were significantly different from those of the control after exposure for 14 d. However, alanine aminotransferase levels showed changes only after exposure to pesticides for 28 d. The biochemical parameters were separated during the experimental period (14 d versus 28 d) by principal component analysis. Based on variable importance plots, blood urea nitrogen/creatinine ratio, blood urea nitrogen, glucose, and gamma-glutamyl transpeptidase are candidate biomarkers for tebuconazole exposure. The residual levels were observed at T4 (12.5 mg/kg bw/d) and T5 (25 mg/kg bw/d) in the liver and fat tissues, respectively. Fibrosis increased in the liver, kidney, and fat tissues, depending on the tebuconazole concentration. In conclusion, the residue limits of tebuconazole and the physiological changes caused by dietary tebuconazole in pigs provide important information for establishing maximum residue limits of pork and pork products.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.25 no.4
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• pp.17-27
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• 1980

All types of weed community became dominant by Monochoria vaginalis (Burm.) Presl. as the nitrogen level increased. The importance value (IV.) of M vaginalis rapidly increased with increasing nitrogen level whereas the LV. of other weed species decreased. At the 10 $\times$ 10cm plant spacing, M. vaginalis was almost totally suppressed at all nitrogen levels. At the 20 $\times$ 20cm plant spacing, the degree of suppression declined with increasing nitrogen level. At the 30 $\times$ 30cm plant spacing, there was no suppression of M vaginalis at the highest nitrogen level. The yield obtained at the 10 $\times$ 10cm plant spacing when M. vaginalis was present was not significantly different from that obtained from the weed free plot for all rice cultivars while it was significantly reduced by M vaginalis competition at the 20 $\times$ 20cm and 30 $\times$ 30cm plant spacings. The difference in yield caused by M vaginalis competition was primarily due to a reduction in the number of panicles at all nitrogen levels. There was a high negative correlation between grain yield and weed weight at heading. The yield reduction due to weed competition varied depending upon the nitrogen level.

• Asian-Australasian Journal of Animal Sciences
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• v.31 no.10
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• pp.1619-1626
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• 2018

Objective: This study was an attempt to investigate the effect of levels of fungal (Lentinus sajor-caju) treated oil palm fronds (FTOPF) on digestibility, rumen fermentation, and nitrogen balance in goats. Methods: Four 16 month old male crossbred (Thai Native${\times}$Anglo Nubian) goats with initial body weights of $33.5{\pm}1.7kg$ were randomly assigned according to a $4{\times}4$ Latin square design. Four levels of FTOPF were assigned for feed intake. The experimental treatments consisted of 0%, 33%, 67%, and 100% of oil palm fronds (OPF) being replaced by FTOPF. Results: The results revealed that total dry matter intake and nutrient intake were not influenced (p>0.05) by the inclusion of FTOPF. However, the efficiency values of the digestibility of the dry matter, organic matter, crude protein, neutral detergent fiber, acid detergent fiber, and acid detergent lignin on FTOPF were higher (p<0.05) in treatments with 33%, 67%, and 100% of FTOPF compared with 0% of FTOPF. FTOPF feeding did not change the rumen pH, temperature, and $NH_3-N$. However, the FTOPF levels did affect the total volatile fatty acid (VFA), molar proportion of acetate, propionate, butyrate, ratio of acetic (propionic acid and acetic) plus butyric (propionic acid), and production of $CH_4$. The totals of VFA and propionate was lower in goat fed with 0% of FTOPF than in those of the other groups (p<0.05). The amount of nitrogen retention based on g/d/animal or the percentage of nitrogen retained was the lowest the goat fed with 0% of FTOPF (p<0.05), whereas nitrogen intake, excretion, and absorption were not changed among treatments. Conclusion: Based on this study, FTOPF could be effectively used as an alternative roughage source in total mixed ration diets, constituting at least up to 100% of OPF.

• Asian-Australasian Journal of Animal Sciences
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• v.20 no.3
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• pp.412-417
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• 2007

An experiment was conducted to determine the distribution of nitrogen-15 in tissues of laying hens receiving different levels of $^{15}N$-CCC in diets. Twenty brown laying hens were divided into four groups and randomly assigned into one of four dietary treatment groups consisting of 0, 5, 50 and 100 ppm $^{15}N$-CCC inclusion. The hens were individually fed with the $^{15}N$-CCC diets in battery cages for 11 days and then all hens restored to feeding on the control diet for 7 days. After eleven days, eight hens were slaughtered, and the others were slaughtered seven days after $^{15}N$-CCC diets withdrawal. Samples of blood, liver, heart and meat were collected and their $^{15}N$ contents were determined. The ${\delta}^{15}N$ excess (${\delta}^{15}N$-ex) and atom percentage excess in $^{15}N$ were calculated. The ${\delta}^{15}N$-ex and atom percentage excess $^{15}N$ increased significantly (p<0.05) with increasing levels of $^{15}N$-CCC in diets in all tissues after feeding $^{15}N$-CCC diets for eleven days. The highest concentration of ${\delta}^{15}N$-ex and atom percentage excess $^{15}N$ were detected in blood, followed in order by liver, heart and thigh meat. The concentrations reduced significantly (p<0.05) after $^{15}N$-CCC diets were withdrawn. Comparison between treatment groups showed that ${\delta}^{15}N$-ex and atom percentage excess $^{15}N$ were still higher in hens that had been fed diets with higher levels of $^{15}N$-CCC. This study showed that nitrogen-15 was distributed in blood, liver, heart and meat of laying hens.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.33 no.1
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• pp.38-47
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• 1988

To determine the response of soybean cultivars to nitrogen in relation to acidity, nodulation, content of allantoin nitrogen and major chemical compositions of soybean plants were investigated with two cultivars under two levels of soil pH and four levels of nitrogen fertilization in a field and nutri-culture experiments. Nodulation and contents of allantoin nitrogen, total nitrogen, potassium, calcium, magnesium and manganese of soybean plants decreased under acidic condition, but the degree of decrease was smaller in the variety Jangbaegkong compared to the variety Danyeobkong. On the other hand, nodulation, content of allantoin nitrogen and potassium decreased with increased nitrogen fertilization, particulary under pH 7 condition. Nodulation was positively correlated with the content of allantoin nitrogen of soybean plants. The content of total nitrogen of soybean plants increased with increased nitrogen fertilization, and this tendency was remarkable under acidic condition and in the variety Jangbaegkong.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.50 no.1
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• pp.1-4
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• 2005

This experiment was conducted to investigate the soil nitrogen credit of biological nitrogen fixation (BNF) and the nitrogen balance of soybean in soybean-barley cropping systems. Soybean cultivar, Shinpaldalkong2 and barley cultivar, Olbori, were used in soybean mono-cropping (SM), barley monocropping (BM), and barley­soybean double cropping system. The barley-soybean double cropping system was treated with two different levels of nitrogen fertilizers, 0 nitrogen fertilizer (BS-F0), and standard nitrogen fertilizer (BS-F1). Nitrogen and organic matter concentrations in soil of BS-F1 plot on October, 2001 were increased $4.8\%\;and\;5.9\%$, respectively, compared with those on October, 2000. The ranges of BNF rate in soybean were $69.1\~ 88.2\%$ in two years, and the rate was the highest in BS-F0 plot and the lowest in SM plot. The ranges of nitrogen harvest index (NHI) in all treatments were $83.9\~86.7\%$. The yield was 270 kg/10a in BS­F1 plot and 215 kg/10a in BS-F0 plot. However, the nitrogen balances were +0.6 kg/10a of gain of soil nitrogen in BS-F0 plot and -0.4 kg/10a of loss of soil nitrogen in BS-F1 plot. In comparisons of SM and BS-F1 plots, although the seed yields were similar in two plots, the loss of soil nitrogen was higher in SM than BS-F1 plot. Overall, our results suggest that barley-soybean double cropping system was more effective in respect to seed productivity and soil nitrogen conservation than soybean monocropping system, and the N credit to following crops by soybean cultivation was identified in soybean double cropping system.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.50 no.5
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• pp.340-345
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• 2005

A field experiment was conducted to assess the growth characteristics, seed and oil yield of two cultivars of soybean (G max (L.) Merr.) cv. PK-416 ($V_1$) and cv. PK-1024 ($V_2$) in relation to sulphur and nitrogen nutrition. Six combinations ($T_1-T_6$) of two levels of sulphur (0 and 40 kg $ha^{-1}$) and two levels of nitrogen (23.5 and 43.5 kg $ha^{-1}$) were applied to the two soybean cultivars as nutrients. Results indicated significant effect of sulphur and nitrogen, when applied together, on the growth characteristics, yield components, and seed and oil yield. Maximum response was observed with treatment $T_6$ (having 40 kg S and 43.5 kg N $ha^{-1}$). Seed and Oil yields were increased 90 and $102\%$ in $V_1$> and 104 and $123\%$ in $V_2$, respectively as compared to the control i.e. $T_1$ (having 0 kg S and 23.5 kg N $ha^{-1}$). Positive responses of S and N interaction on leaf area index, leaf area duration, crop growth rate and biomass production were also observed. The results obtained in these experiments clearly suggest that balanced and judicious application of nitrogen and sulphur can improve both seed and oil yield of soybean cultivars by enhancing their growth.

• Asian-Australasian Journal of Animal Sciences
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• v.13 no.12
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• pp.1667-1673
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• 2000

The climate and marketing system of raw milk in Taiwan create problems in balance feeding of protein and energy in lactating cows in Taiwan. Level of urea nitrogen both in bulk milk and serum reflects ruminal protein degradation and post-ruminal protein provision, whereas milk protein concentration responds to dietary energy intake and bacterial protein production in the rumen. Establishment of a range of reference standards in milk protein and urea nitrogen levels can be applied as a noninvasive economical feeding guide to monitor the balance of protein and energy intake. Standard reference levels of 3.0% milk protein and 11-17 mg/dL milk urea nitrogen (MUN) were established. Level of milk protein below 3.0% is regarded as indicating inadequate dietary energy whereas MUN below or above the range is regarded as a deficiency or surplus in dietary protein. Results from analysis of bulk a milk samples collected from 174 dairy herds over Taiwan showed that only one quarter (25.29%) of the herds received a balanced intake of protein and energy, 33.33% adequate protein with energy inadequate, 22.99% herds in protein surplus with energy inadequate, 10.35% herds in protein surplus with energy adequate, 4.6% protein deficiency with energy adequate, and 3.45% herds with both protein and energy inadequate. Energy inadequate herds accounted for 60% of the total dairy herds in Taiwan with 56% adequate, 38% surplus and 6% inadequate in protein. In comparing milk sampled from bulk milk on different seasons from Lee-Kang area in the southern Taiwan, the concentrations of milk fat and milk protein were significantly higher in the cool season (February) than in the warm season (August) (p<0.05), whereas the urea nitrogen in the milk was significantly lower in the cool season than in the warm season (p<0.05). This indicated that lactating cows had excess protein and/or inadequate energy intake in the warm season in this area. It appears that the major problem feeding in lactating cows is energy intake shortage, especially during the warm season in Taiwan.

• Journal of The Korean Society of Grassland and Forage Science
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• v.14 no.2
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• pp.88-92
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• 1994

This study was carried out to investigate the effects of lime(0, 250, 500 and 1,000 kg/lOa) and phosphate (0, 17 and 34 kg/lOa) applications on growth and nitrogen fixation of alfalfa (Medicago sativa L.). The phosphate applications improved(p<0.05) shoot, root and root nodule dry weight of Alfalfa and acetylene reduction activity of alfalfa with increasing levels of phosphate in the soil pH 6.2 at 7 and 12 weeks(ear1y bloom) after sowing, but these were not influnced with lime applications. Total nitrogen content of each part of alfalfa was increased with lime application, but was not significantly different by levels of lime application at 7 weeks after sowing. Application of phosphate did not affect total nitrogen content of each part of alfalfa. Total nitrogen contents of each part of alfalfa were not significantly different between lime and non-lime application at 12 weeks(ear1y bloom) after sowing but were decreased(p<0.05) with phosphate application(p< 0.05). These results suggest that lime(250 kg/lOa) and phosphate(34 kg/lOa) applications are effective for the growth and the nitrogen fixation of alfalfa at the soil pH 6.20.

• Korean Journal of Soil Science and Fertilizer
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• v.30 no.1
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• pp.84-88
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• 1997

To establish N fertilizer recommendation method based on nitrate content of the soil for the Chinese cabbage grown in the plastic film house. Chinese cabbage was grown in the pots containing the plastic film house soils with various levels of $NO_3{^-}-N$ and different levels of fertilizer N. The nitrate nitrogen showed the positive correlation with nitrogen uptake amount by plant and the negative correlation with fertilizer nitrogen use efficiency of plant. The content of nitrate nitrogen in soil for maximum yield of Chinese cabbage was 310 mg/kg. An equation for the recommendation of fertilizer N for Chinese cabbage based on $NO_3{^-}-N$ in the soil was suggested.