• Title/Summary/Keyword: nitrogen levels

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Studies on the Red Pigment Produced by Monascus sp. in Submerged Culture. Part I Isolation of Strain and Cultural Conditions of Pigment Produced (액침진탕 배양에 의한 Monascus sp..가 생산하는 적색색소에 관한 연구 제1보 균주의 분리 및 색소생산 배양 조건)

  • 김현수;김두현;양호석;변유량;유주현
    • Microbiology and Biotechnology Letters
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    • v.7 no.1
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    • pp.23-30
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    • 1979
  • Fungi which produce red pigment were isolated from tapioca chips, Korean Koji, air, and plant leaves. Among the Fungi, T-1 strain was selected as test strain. This strain was identified as a Monatcus sp. by the morphological propeties. Various culture conditions, and physical and physiological characteristics of red pigment were studied. According to the studies of culture conditions, optimum condition was found to be pH 6.5; 4 days of incubation; temperature, 32~33 c: 3.5% of Tapioca chips powder as carbon source, 0.2% of sodium nitrate as nitrogen source and 100 ml of medium in the 500 ml Erlenmyer flask at a rotary shaker (rpm180) as aeration condition. Also effective levels of vitamins, amino acids and inorganic compounds was found to be l$\mu\textrm{g}$/ml of folic acid and niacin; 0.3% of L-arginine, L-glutamic acid and L-proline; and 0.001% of manganese dioxide giving good results.

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Toxicological effects of perfluorooctanoic acid in rats (랫드에서 Perfluorooctanoic Acid의 독성에 관한 연구)

  • Kim, Yong-Hoon;Cho, Eun-Sang;Kim, A-Young;Kim, Sung-Hwan;Park, Min-Sik;Cho, Sung-Whan;Ryu, Si-Yun;Jung, Joo-Young;Son, Hwa-Young
    • Korean Journal of Veterinary Research
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    • v.48 no.3
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    • pp.347-355
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    • 2008
  • Perfluorooctanoic acid (PFOA), a member of the perfluoroalkyl acids that have wide commercial applications, is persistent organic pollutants widely spread throughout the environment and human population. But little is known about the adverse biological effects of the PFOA. In the present study, the toxicological effects of PFOA were investigated in rats. Sprague-Dawley rats (N = 10 in each group) were orally administered with PFOA in drinking water for 4 weeks (0, 100, 200, or 400 ppm in male, and 0, 200, 400, or 800 ppm in female). Three female rats given 800 ppm died during the study. PFOA treatment decreased the body weight gain and increased the liver weights in both genders. Serum biochemical investigations revealed significant increases in the aspartate aminotransferase, alanine transaminase, alkaline phosphatase, blood urea nitrogen, total cholesterol, and total bilirubin in male but in female. Serum estradiol (E2) levels were increased in all treated rats. Histopathologically, hepatocellular hypertrophy around central vein was noted in the liver of treated rats. No significant histopathological changes were noted in other organs. In conclusion, PFOA induced toxicological changes in the liver and increased serum E2 level which was not related to histopathological changes of endocrine and reproductive system.

Effects of Rice Straw Compost Application on Exchangeable Potassium in Long-term Fertilization Experiments of Paddy Soils

  • Kim, Myung-Sook;Park, Seong-Jin;Lee, Chang-Hoon;Ko, Byong-Gu;Yun, Sun-Gang
    • Korean Journal of Soil Science and Fertilizer
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    • v.49 no.2
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    • pp.194-199
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    • 2016
  • In an experiment conducted at the research field of the National Institute of Agricultural Science, we investigated the effects of mineral fertilizer and rice straw compost on exchangeable potassium and K balances, and rice grain yield under a rice single system. The treatments were no fertilization (No fert.), inorganic fertilization (N), inorganic fertilizer (N, P, K) plus rice straw compost at rates of 7.5, 15.0, 22.5, and $30.0ton\;ha^{-1}$ (NPKC7.5, NPKC15.0, NPKC22.5, and NPKC30.0, respectively). The inorganic fertilizers(N, P, K) were added with standard fertilizer application rate in which nitrogen (N), phosphate ($P_2O_5$), and potassium ($K_2O$) were applied with $75{\sim}150kg\;ha^{-1}$, $70{\sim}86kg\;ha^{-1}$, $75{\sim}86kg\;ha^{-1}$, respectively. Exchangeable potassium for NPKC15.0 NPKC22.5, and NPK30.0 treatments was higher by $0.05{\sim}0.19cmol_c\;kg^{-1}$ than that of NPKC7.5 treatment. Increasing levels of rice straw compost resulted in an increase in the K balance from - $19.9kg\;ha^{-1}yr^{-1}$ (No fert.) to $41.9kg\;ha^{-1}yr^{-1}$ at NPKC22.5 treatment and $62.9kg\;ha^{-1}$ at NPKC30.0 treatment. Continuous application of rice straw compost with NPK fertilizers affected significantly the rice grain yields. The result of the study imply that the application of more than $22.5ton\;ha^{-1}$ of rice straw compost with NPK fertilizers are recommended as the best fertilization practice for enhancement of crop production and K supplying power of soil in the continuous rice cropping system.

The Relation between Fertilization Practices and Functional Metabolites of Crops: A Review

  • Lim, Jung-Eun;Cho, Min-Ji;Yun, Hye-Jin;Ha, Sang-Keun;Lee, Deog-Bae;Sung, Jwa-Kyung
    • Korean Journal of Soil Science and Fertilizer
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    • v.49 no.2
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    • pp.168-180
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    • 2016
  • Various researches on the effects of fertilization levels on functional metabolites in crop have been conducted. This review summarizes the previous studies on the relation between fertilization supply and accumulation of metabolites (phenolics, carotenoids, ascorbic acid and glucosinolates) which function as antioxidants in crop. The accumulation of phenolic compounds is related to the activation of phenylalanine ammonia lyase (PAL) in phenylpropanoid pathway. Most of the previous studies discuss that low nitrogen (N) supply activates PAL, thereby increasing the synthesis of phenolics. Similarly, high N supply leads to a decrease in ascorbic acid because of the shading effect derived from the accelerated vegetative growth under high N level. Unlike the phenolics and ascorbic acid, carotenoids are accumulated with increasing N supply. In this regard, the previous studies explain that N is a main element closely associated with formation of key enzyme for the synthesis of carotenoids. Glucosinolates are generally increased under decreasing N supply and increasing S supply. Although the previous studies show similar trends about the accumulation of metabolites by nutrient level, they also suggest that many other factors including crop types, cultivars, cultural environment (water, temperature, light, etc.) influence the accumulation of functional metabolites in crop.

Assessments of the Nutrient Losses in the Sloped Farm Land (경사지 밭토양에서의 양분유실량 평가)

  • Jung, Pil-Kyun;Eom, Ki-Cheol;Ha, Sang-Keon;Zhang, Yong-Seon;Hur, Seung-Oh
    • Korean Journal of Soil Science and Fertilizer
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    • v.42 no.spc
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    • pp.47-50
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    • 2009
  • Nutrient losses, especially nitrogen and phosphorus, in agricultural runoff can contaminate surface and ground water, leading to eutrophication. Thus, erosion control is crucial to minimizing nutrient losses from agricultural land. Assessments of various erosion control practices were carried out under various cropping system, soil management practices, and slope conditions by means of a lysimeter study and under artificial rainfall. Soil and nutrient losses were monitored in a small agricultural field to evaluate the soil conservation practices. Nutrient losses occur in runoff and leachate (dissolved nutrient) and in sediments (particulate nutrient). Dissolved nitrates accounted for the majority (about 90%) of nitrate transport within the soil. Particulate phosphate in sediments represented the majority (60% to 67%) of phosphate transport. Recently, engineering and agronomic erosion-control practices haver been used to reduce erosion problems in fields on slopes. These practices reduced soil loss, runoff, and nutrient loss to 1/6, 1/2,and 1/3 their original levels, respectively. Bioavailable particulate phosphate in sediments represents a variable but longterm source of phosphate for algae. Dissolved nitrate and phosphate are immediately available for algal uptake, so reducing fluxes of these nutrients should also reduce the risk of eutrophication.

Cryopreservation of in Vitro Grown Shoot Tips of Sweet Potato (Ipomoea batatas L.) by the Encapsulation-Vitrification Method

  • Yi, JungYoon;Lee, GiAn;Lee, YoungYi;Gwag, JaeGyun;Son, EunHo;Park, HongJae
    • Korean Journal of Plant Resources
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    • v.29 no.6
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    • pp.635-641
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    • 2016
  • Sweet potato (Ipomoea batatas L.) shoot tips grown in vitro were successfully cryopreserved by encapsulation-vitrification. Encapsulated explants are very easily manipulated, due to the relatively large size of the alginate beads, and a large number of samples can be treated simultaneously. In this study, the effects of sucrose preculture, cryoprotectant preculture, and post-warm recovery media on regrowth, following liquid nitrogen (LN) exposure, were investigated to establish an efficient encapsulation-vitrification protocol for sweet potato. Shoot tips of plants grown in vitro were precultured in 0.3 M sucrose for 2 d before encapsulation. Encapsulated shoot tips were pre-incubated in liquid MS (Murashige and Skoog) medium containing 0.5 M sucrose for 16 h, before preculturing in sucrose-enriched medium (0.7 M sucrose) for 8 h. Shoot tips were osmoprotected with 35% plant vitrification solution 3 (PVS3) for 3 h, before being dehydrated with PVS3 for 2 h at $25^{\circ}C$. The encapsulated and dehydrated shoot tips were transferred to 2 mL cryotubes, suspended in 0.5 mL PVS3, and plunged directly into liquid N. High levels of shoot formation were obtained for the cv. Yeulmi (65.7%) and Yeonwhangmi (80.3%). The regrowth rates of cryopreserved samples in Yeulmi (78.9%) and Yeonwhangmi (91.3%), following culture on ammonium-free MS medium for 5 d, were much higher than those cultured on standard MS medium (65.7% and 80.3%, respectively). This encapsulation-vitrification is a promising method for the long-term preservation of sweet potato.

ORTHROGNATHIC SURGERY IN SEVERE CHRONIC RENAL FAILURE PATIENT (중증의 만성신부전 환자에서의 악교정 수술)

  • Shin, Sang-Hun;Kim, Ki-Hyun;Jeung, Suck-Young;Park, Sung-Hwan;Kim, Cheol-Hun
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
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    • v.26 no.6
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    • pp.652-657
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    • 2000
  • The kidneys play a vital role in the maintenance of normal body fluid volumes and in the composition of the extracellular fluid compartments. There are normally more than 2 million functioning glomeruli that regulate total body water and solute concentrations. As renal failure progresses, there is a decrease in the number of functioning nephrons. Chronic renal failure(CRF) is the consequence of a multitude of diseases that cause permanent destruction of the nephron. Azotemia is an elevation in blood urea nitrogen(BUN) and serum creatinine levels subsequent to a decreased glomerular filtration rate(GFR), which results in uremia. This loss of renal function can cause functional and metabolic abnomalities of body. For this problem, oral & maxillofacial surgeons have demanded to routinely treat patients with CRF. However, there has not been a reported case of orthognathic surgery by bilateral sagittal split ramus osteotomy(BSSRO) in patients with CRF, which can cause multiple complications in healthy patients. We report developmental mechanism of complication associated with CRF and preop. and postop. care of orthognathic surgery by BSSRO in Cl III patient with severe chronic renal failure.

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Studies on the Growthgain of Rats Fed on Rice Diet Adding Buckwheat (백미(白米)에 교맥분(蕎麥粉)을 섞은 사료(飼料)로 사육(飼育)하였을때의 Rat 의 Growthgain 에 대(對)하여)

  • Lee, Hyun-Ki
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.1 no.1
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    • pp.1-4
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    • 1972
  • The following is the result of feeding ad libitum the Wistar strain rats (male) divided into 4 groups-(a) casein group, (b) rice diet group, (c) diet group with a mixture of 80% rice and 20% buckwheat and (d) diet group of equal nitrogen levels of rice and buckwheat. Compared with the control group, the diet group given a mixture of 80% rice and 20% buckwheat put on weight slower, but quiker than the rest two. Particulariy after 10 days it was found to gain greater weight. Therefore, the discovery is that as proved by theory such a mixed food with a proportion of 20% buckwheat and 80% rice can improve the protein score even in the experiment of feeding animals.

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Property Changes of Vertical Soil Layers Planted with Zelkova serrata(Thunberg) Makino on the Reclaimed Land from the Sea in the Gwangyang Bay, Korea (광양만 임해 매립지 느티나무 식재지 토양의 수직적 특성 변화)

  • Kim Do-Gyun;Kim Yong-Shik
    • Journal of the Korean Institute of Landscape Architecture
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    • v.33 no.2 s.109
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    • pp.60-70
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    • 2005
  • This study was carried out to elucidate the vertical characteristics of soil properties at six planted sites of land reclaimed from the sea, in Gwangyang Bay, Jeollanam-do Province, Korea. Based on the types of planting site, the chemical properties of the vertical soil layers varied. The vertical variation was great in the planting sites $Z_1\;and\;Z_2$, but less varied in the mounded planting sites $Z_3,\;Z_5,\;and\;Z_6$. Major reasons for the vertical variation in soil chemical properties included differences in the accumulation of organic matter, soil disturbance by heavy construction equipment, and heterogeneity of soil properties between soil horizons. As soil depths increased, soil salts varied. The electrical conductivity (ECe) increased in the lower areas of planting sites $Z_1\;and\;Z_2$, and the disturbed, saline planting site $Z_3$, but decreased in the lower areas of $Z_3,\;Z_5,\;and\;Z_6$. These tendencies did not coincided with exchange cation concentrations $(Na^+,\;K^+,\;Mg^{++},\;Ca^{++})$. Both total carbon (T-C) and total nitrogen (T-N) accumulated more in the lower areas of planting sites than in the higher areas, and levels were higher closer to the surface than in the soil depths. It is supposed that these tendencies are related to the accumulation of fallen leaves or other organic matter at the soil surface, and the soil chemicals then slowly move downward from the surface. Impediments to tree growth included soil hardiness, high soil salinity and exchangeable cation concentration, low soil moisture content, acidic or alkaline soil, low organic matter, heterogeneity of soil texture and establishment of soil stratification.

Characteristics of Water and Sediment Qualities in the Oncheon Stream, Busan during Summer Rainy Season (부산 온천천의 하계 우수기 수질 및 퇴적물 환경 특성)

  • Lee, Young-Hyeong;Moon, Changho;Kang, Hyun-Jung;Choi, Seong-Ryul;Kim, Suk-Hyun
    • Journal of Environmental Science International
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    • v.29 no.6
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    • pp.659-672
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    • 2020
  • Water and sediment qualities were investigated in the Oncheon Stream and at the Wondong bridge of the Suyoung River, during the summer rainy season, 2019. Dissolved oxygen (DO) showed the lowest levels at 4.7 and 5.0 m/L, and biogeochemical oxygen demand (BOD) showed the highest at 5.3 mg/L downstream where the tributary flows into the main river. Chemical oxygen demand (COD) increased from 2.0 to 5.9 mg/L on average as it flowed downstream, The COD/BOD ratio decreased gradually as it flowed downstream, reaching 1.0. However, COD/BOD ratio at the Wondong bridge was 5.8-22.2, indicating that easily biodegradable and non-biodegradable organic matter flows into the Oncheon Stream and Suyoung River, respectively. Total nitrogen (T-N) / total phosphorus (T-P) ratio tended to decrease from 72 to 21 as it flowed downstream, measuring 71 to 86 at the Wondong bridge. The water quality index (WQI) generally improved better than grade IV after heavy rainfalls. However, DO and T-P were the parameters that deteriorated the WQI. Ignition loss (IL), COD, T-N, and T-P of sediments had distribution of 1.44 ± 1.01%, 0.35 ± 0.16%, 43 ± 63 mg/kg, and 10.9 ± 21.9 mg/kg, respectively. These were several times lower than the annual averages of IL, T-N, and T-P in 2017 before the dredging project was conducted in the first half of 2018.