• Asian-Australasian Journal of Animal Sciences
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• v.30 no.6
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• pp.797-803
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• 2017

Objective: Effects of nitrogen supplementation associated with different levels of starch on voluntary intake, digestibility, and rumen and metabolic characteristics of cattle fed low-quality tropical forage (Brachiaria decumbens hay, 7.4% crude protein, CP) were evaluated using ruminal and abomasal cannulated steers. Methods: Five European${\times}$Zebu young bulls (186 kg body weight, BW) were distributed according to a $5{\times}5$ Latin square. The following treatments were evaluated: control, supplementation with 300 g CP/d (0:1), supplementation with 300 g starch/d and 300 g CP/d (1:1), supplementation with 600 g starch/d and 300 g CP/d (2:1), and supplementation with 900 g starch/d and 300 g CP/d (3:1). A mixture of nitrogenous compounds provided 1/3 from true protein (casein) and 2/3 from non-protein nitrogen (mixture of urea and ammonium sulphate, 9:1) was used as the nitrogen supplement. In order to supply energy a unique source of corn starch was used. Results: Supplements increased (p<0.05) dry matter intake, but did not affect (p>0.05) forage intake. There was a cubic effect (p<0.05) of starch on voluntary intake. This was attributed to the highest forage intake (g/kg BW) when using the 2:1 starch:CP ratio. Supplements increased (p<0.05) organic matter (OM) digestibility, but did not affect (p>0.05) neutral detergent fibre corrected for ash and protein (NDFap) digestibility. There was a positive linear effect (p<0.05) of the amount of starch supplemented on OM digestibility. Total NDFap digestibility was not affected (p>0.05) by the amount of supplemental starch. Ruminal ammonia nitrogen concentrations were higher (p<0.05) in supplemented animals, however, a negative linear effect (p<0.05) of amount of starch was observed. Supplements increased (p<0.05) the nitrogen balance (NB) and efficiency of nitrogen utilization. These effects were attributed to increased body anabolism, supported by higher (p<0.05) serum concentration of insulin-like growth factor 1. Increasing the amount of starch tended (p<0.06) to linearly increase the NB. In spite of this, there was a highest NB value for the 2:1 starch:CP ratio amongst the treatments with supplementation. Conclusion: Nitrogen supplementation in cattle fed low-quality tropical forage increases nitrogen retention in the animal's body. An additional supply of starch increases nitrogen retention by increasing energy availability for both rumen and animal metabolism.

• Korean Journal of Agricultural Science
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• v.42 no.4
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• pp.431-437
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• 2015

Recently, assessment of nitrogen balance has been required for environmental agriculture. Nutrient management using organic matters in farmlands has been strongly required as a means of extending resource-cycling agriculture and reduction of nitrogen balance. Organic matters-derived nutrients and soil-available nitrogen should be necessarily considered to manage nutrient balance in soil-plant system. In this study, we reviewed the amount of N supply according to types of organic matter such as livestock compost and green manure in arable land. In case of applied livestock compost in soil, nitrogen mineralization was influenced by nitrogen amount of livestock manure and mixed materials. And nitrogen mineralization of green manure in arable land was influenced by types of crop and return period of green manure because of change of C/N ratio. Also, nitrogen supply by organic matter in arable land can be changed by environmental factors such as temperature, moisture in soil. Therefore, nitrogen supply according to C/N ratio of organic matter and analysis method for estimation of soil nitrogen supply availability should be evaluated to set up the nutrient management model.

• Journal of Korean Society of Forest Science
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• v.84 no.2
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• pp.198-206
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• 1995

The objective of this study was to determine the rate of nitrogen mineralization at clearcut and uncut in oak(Quercus rubra L.) and pine(Pinus resinosa Ait.) stands. The study sites were located in northern Lower Michigan, U.S.A. Nitrogen mineralization rates in the top 15cm of mineral soil were examined during the 1991 and 1992 growing seasons(May-October) using an in situ buried tag technique. diet nitrogen mineralization in oak stands over the course of both growing seasons was 67kg/ha in the clearcut and 30kg/ha in the uncut stands. In contrast, net nitrogen mineralization in pine stands was 27kg/ha and 13kg/ha for the same treatments. Total net nitrogen mineralization rates were always higher in oak stands than in pine stands. Extractable $NO_3^-$ before and after one month soil incubation in both oak and pine stands was generally not detectable in the uncut stands, but in the pine clearcut treatment(nitrification over the course of both growing seasons : 3.3kg/ha). The results indicated that : 1) substantial increases in the amount of available soil nitrogen occurred following clearcuts in both stands : and 2) the loin rates of nitrification may be an important mechanism for retention of nitrogen in both oak and pine stands.

• Ecology and Resilient Infrastructure
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• v.5 no.1
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• pp.11-18
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• 2018

Phosphorous remains as one of the most limiting nutrients to plant growth, second only to nitrogen. Research on use of biochar as a soil amendment for available phosphorus in temperate calcareous soils has limited studies compared with to tropical acidic soils. An incubation experiment to assess phosphorous availability in a biochar amended calcareous soil under inorganic (Fused superphosphate, FSP) and organic fertilizer (bone meal, BM) and respectively, at the dose of 40, 80 and $120mg\;P\;kg^{-1}$ was carried out. Soil was incubated at $25^{\circ}C$ for 70 days. Results show that the rate of increase in available P was proportional to the fertilizer application rate with or without biochar amendment. Biochar did not have a significant effect on soils amended with either fertilizeron the values of available P. However, time had a significant effect (p<0.001) on the amount of available P during the incubation period. Inorganic fertilizer treatments had recorded high amount of available P with time compared to organic fertilizer treatments. Organic fertilizer treatment sample were significantly not different from control and for most of the incubation time biochar acted as a soil conditioner. Further research is required to understand the holistic and long-term effect of biochar.

• Asian-Australasian Journal of Animal Sciences
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• v.21 no.11
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• pp.1624-1628
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• 2008

Experiments were conducted to evaluate the nutritional value of yellow-seeded rapeseed meal (YRSM). In the first experiment nutrient retention was recorded by 48 Arbor Acres-broiler chickens (28-d old) to determine AMEn (nitrogen-corrected apparent metabolizable energy), coefficient of apparent protein digestibility based on ileal digesta nitrogen, excreta nitrogen and uric acid nitrogen. The second experiment was carried out with 304 Arbor Acres-broiler chickens to compare effects of SBM (soybean meal) and YRSM on performance, carcass and digestive tract status. In the control treatment, SBM was replaced by graded levels of YRSM at 15, 22.5 and 30% of diet. Digestibility of YRSM protein was significantly lower (p<0.001) than SBM protein. The protein digestibility based on ileal measurement was significantly higher (p<0.001) than protein digestibility from excreta samples. There was no significant difference (p>0.001) between ileal and excreta digestibility of protein based on uric acid. AMEn as a fraction of gross energy was 0.54 in SBM and 0.45 in YRSM. With the exception of 30% YRSM, other YRSM treatments resulted in major effects on length and weight of the gastrointestinal tract. The results of this study have shown no adverse effect on performance as well as protein digestibility and energy value in response to replacement of SBM by YRSM with the exception of 22.5 and 30% YRSM.

• Journal of Korean Society for Atmospheric Environment
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• v.14 no.E
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• pp.9-17
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• 1998

Oxides of nitrogen play important roles in atmospheric chemistry. Soil has been recognized as a major natural source of NO, and its emission depends on soil parameters such as soil nitrogen availability, soil moisture and temperature. It is necessary to understand effects of these controlling parameters on soil NO emission. In order to understand soil moisture effects on NO emission, variations of NO concentration and existence of its equilibrium concentration were observed from ammonium fertilized Japanese upland soil prepared for different soil moisture conditions. The closed chamber technique was employed for this study. The significant increases in NO with soil moisture were found. Maximum was occurred at sample ID4 (55% of water-filled pore space (WFPS)), but it decreased as soil moisture increased. No significant NO concentration was emitted from soil sample without fertilizer, but there was significant NO in fertilized soil samples. The magnitudes of NO from soil increased with time and reached at steady state within ten minutes approximately. These results suggest that nitrogen input from fertilizer takes charge in the first step of sharp increase in NO emission, and then soil moisture becomes important factor to control NO emission from the soils. NO concentrations from soil were compared to those one-day after the experiment. Results from the comparison analysis suggest that the soil NO flux might have been stimulated by soil disturbances like mixing, and this is much more effective in dry soils rather than in wet soils. It was found that much less NO came out from soils after a day; suggesting that most of NO was released from the soils within a day after fertilizer application during our experiment. The length of NO releasing time span may depend on the amounts of fertilizer applied, soil moisture condition, and other soil physical parameters.

• Microbiology and Biotechnology Letters
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• v.49 no.3
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• pp.432-439
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• 2021

Microbial community plays important roles in the culture environment of mud crab Scylla serrata. One of the environmental management efforts for the cultivation of S.serrata is by stabilizing microorganisms involved in nitrogen cycle process. The availability of dissolved inorganic nitrogen in its culture environment under a recirculating system closely relates to the nitrogen cycle, which involves both anaerobic and aerobic bacterial activities. Anaerobically, there are two major nitrogen compound degradation processes, i.e., denitrification and dissimilatory nitrate reduction to ammonium (DNRA). This study aimed to identify denitrifying and DNRA bacteria isolated from the recirculating cultivation of S. serrata. The water samples were collected from anaerobic filters called close filter system, which is anaerobically conditioned with the addition of varying physical filter materials in the recirculating mud crab cultures. The results showed that three denitrifying bacterial isolates and seven DNRA bacterial isolates were successfully identified. The phylogenetic analysis based on 16S rRNA gene of the denitrifying bacteria revealed that HIB_7a had the closest similarity to Stenotrophomonas daejeonensis strain MJ03. Meanwhile, DNRA bacterial isolate of HIB_92 showed a 100% similarity to Bacillus sonorensis strain N3, Bacillus vallismortis strain VITS-17, Bacillus tequlensis strain TY5, Geobacillus sp. strain DB24, Bacillus subtilis strain A1, and Bacillus mojavensis strain SSRAI21. This study provides basic information denitrifying and DNRA bacterial isolates identity which might have the potential to be applied as probiotics in aquaculture systems in order to maintain optimal environmental conditions.

• Weed & Turfgrass Science
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• v.3 no.4
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• pp.362-369
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• 2014

A developed slurry composting and biofiltration (DSCB) liquid fertilizer could be used for eco-friendly turfgrass management in golf course. This study was conducted to evaluate the growth effect of application intervals of DSCB in Kentucky bluegrass based on turf color index, chlorophyll index, clipping yield and uptake and availability of nutrient. Treatments were designed as follows; non-fertilizer (NF), control (CF) and DSCB treatments which were applied a every 15 days (DSCB), every 30 days (2DSCB) and 60 days (4DSCB-1: April, June, August; 4DSCB-2: May, July, September). Turf color indexes of DSCB and 2DSCB were higher than CF, but these chlorophyll indexes similar to CF. The clipping yield and uptake and availability rate of nitrogen and potassium in turfgrass were increased in 2DSCB. These results suggested that application of DSCB improved turf quality and growth by prompting an uptake and availability of nutrients in Kentucky bluegrass and its application interval was 1time per month.

• Korean Journal of Soil Science and Fertilizer
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• v.49 no.5
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• pp.510-516
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• 2016

Soil respiration has been recognized as a key factor of the change of organic matter and fertility due to the carbon and nitrogen mineralization. In this study, we evaluated the effect of soil respiration on the light fraction-C and inorganic N content depending on temperature in soil applied with organic matter. Soil respiration was calculated by using total $CO_2$ flux released from soil applied with $2Mg\;ha^{-1}$ of rice straw compost and rye for 8 weeks incubation at 15, 25, $35^{\circ}C$ under incubation test. After incubation test, light fraction and inorganic N content were investigated. Rye application dramatically increased soil respiration with increasing temperature. $Q_{10}$ value of rye application was 1.69, which was higher 27% than that of rice straw compost application. Light-C and $NO_3-N$ contents were negatively correlated to soil respiration. Light-C in rye application more decreased than that in rice straw compost with temperature levels. These results indicate that temperature sensitivity of soil respiration could affect soil organic mater content and N availability in soil due to carbon availability. Also, light fraction would be useful indicator to evaluate decomposition rate of organic matter in soil under a short-term test.

• Fisheries and Aquatic Sciences
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• v.25 no.11
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• pp.537-548
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• 2022

Apparent digestibility coefficients (ADCs) of dry matter, crude protein, crude lipid, nitrogen-free extract, energy and essential amino acids in animal-based feed ingredients were determined for olive flounder (Paralichthys olivaceus). A reference diet (RF) was formulated to contain 1.0% chromic oxide (Cr₂O₃) as an inert indicator. Nine test diets were formulated to contain RF and one of the feed ingredients (pollock meal [PM], jack mackerel meal [JMM], anchovy meal [AM], cod meal [CM], sardine meal [SM], sand eel meal [SEM], tuna meal [TM], meat meal [MM] and squid liver meal [SLM]) at a 7:3 ratio in each diet designated as PM, JMM, AM, CM, SM, SEM, TM, MM and SLM, respectively. Olive flounder, averaging 150 ± 8.0 g, were stocked at a density of 25 fish per tank in 400-L fiberglass tanks attached with fecal collection columns. Feces were collected from triplicate groups of fish one time a day for four weeks. Dry matter and crude protein ADCs of CM and SEM were significantly higher than the other tested ingredients. Lipid ADCs of JMM, CM and SEM were significantly higher than the other test ingredients. Energy ADCs of CM and SEM were significantly higher than that of the other tested ingredients. The availability of amino acids in CM was generally higher than the other animal protein sources. PM exhibited the lowest amino acid availability among the treatments. Interestingly, MM exhibited significantly higher nutrient digestibility than several marine-based ingredients. However, CM and SEM are seeming to be highly digestible and effective to use in olive flounder diet compared to the other tested ingredients. Overall, the results of this study provide information about the bioavailability of nutrients and energy in animal feedstuffs to apply when formulating cost-effective practical feeds for olive flounder.