• Title/Summary/Keyword: nitrite scavenging and antioxidant activity

Search Result 340, Processing Time 0.025 seconds

Component Analysis and Antioxidant Effects of Youngia sonchifola Max. (고들빼기의 성분분석과 항산화효과)

  • Kim, Mee-Jeong;Park, Hee-Suk;Lee, Chang-Il;Kim, Sung-Hwan;Kim, Pil-Nyeon;Huh, Wan;Lee, Do-Yeong;Son, Jin-Chang
    • Journal of Food Hygiene and Safety
    • /
    • v.25 no.4
    • /
    • pp.354-359
    • /
    • 2010
  • In the present study, we investigated the chemical composition, antioxidant activities and nitrite scavenging ability in leaf and root of Youngia sonchifolia Max. The leaf powder contained 4.3% of water, 53.9% of crude carbohydrate, 21.6% of crude protein, 3.5% of crude fat and 16.7% of crude ash. The root powder contained 4.8% of water, 65.9% of crude carbohydrate, 17.4% of crude protein, 3.2% of crude fat and 8.7% of crude ash. The major mineral elements both in leaf and root powder were potassium, calcium, and magnesium. Contents of unsaturated fatty acids were higher than those of saturated fatty acids both in leaf and root powder. Total polyphenol and flavonoid contents of methanol extract in leaf were 3,922.4 mg/100 g and 1,903.2 mg/100 g respectively. In comparison, total polyphenol and flavonoid contents of methanol extract in root powder were 1,898.4 mg/100 g and 359.8 mg/100 g. The antioxidative activities of several solvents extract of leaf and root powder were investigated by measuring electron-donating ability using 1,1-diphenyl-2-picrylhydrazyl (DPPH). Of the each extracts, ethyl acetate extract of leaf and root powder showed relatively higher antioxidant activity; 94.3% in the leaf powder and 92.9% in the root powder. Nitrite scavenging ability was also highest in the ethyl acetate extract of leaf (45.4%) and root powder (28.8%). These results suggest that ethyl acetate extract of Youngia sonchifolia Max. can be used as a functional materials.

Antioxidants of Pine Needle Extracts According to Preparation Method (제조방법별 솔잎추출물의 항산화성 검토)

  • Kim, Soo-Min;Kim, Eun-Ju;Cho, Young-Suk;Sung, Sam-Kyung
    • Korean Journal of Food Science and Technology
    • /
    • v.31 no.2
    • /
    • pp.527-534
    • /
    • 1999
  • This study was carried out to investigate the effects of pine needle extracts on lipid oxidation and free radical reaction in iron sources reacted with active oxygen species. The results were summarized as follow; the catalytic effects of active oxygen on lipid oxidation in oil emulsion tended to be showed $OH,\;H_2O_2\;and\;KO_2$ in order. At the same time, pine needle extracts itself were tended to be showed a little catalytic effects. Active oxygen scavenging ability of pine needle extracts didn't show, but pine needle extracts played role as a strong chelating agents to bind iron ion if $Fe^{2+}$ ion exist in oil emulsion. The content of $Fe^{2+}$ ion and total iron in CPNP were higher than those of HPNP and FPN. The content of ascorbic acid of FPN showed the highest (87.77 ppm) among several pine needle extracts. Electron donating ability of HPNP and CPNP were 81% and 78%, respectively, which were showed higher content than those of FPN. The SOD-like activity of HPNP showed 44.30%, compared to other pine needle extracts which means the most strong antioxidant reaction. The nitrite scavenging effects were tended to be different, depending on pH value as pH value was increased. Especially, they didn't show the nitrite scavenging effect in pH6.0.

  • PDF

Comparison of Physicochemical Properties and Physiological Activities of Commercial Fruit Juices (시판 과일주스의 이화학적 특성 및 기능성 비교)

  • Chung, Hai-Jung
    • Food Science and Preservation
    • /
    • v.19 no.5
    • /
    • pp.712-719
    • /
    • 2012
  • In this study, the physicochemical and physiological activities of 10 different commercially available juices (apple juice-A: cloud type; apple juice-B: clear type; blueberry juice; grape juice-A; grape juice-B; orange juice-A; orange juice-B; pineapple juice; pomegranate juice; and tomato juice) were investigated. The average pH, titratable acidity, Brix, and reducing sugar were 3.57, 0.57%, $13.1^{\circ}Brix$, and 57.58%, respectively. The total polyphenol and flavonoid contents were within the ranges of 98.29-878.12 ug/mL and 23.82-156.63 ug/mL, respectively, and pomegranate juice showed the highest content, followed by blueberry juice and apple juice-A. In the DPPH radical scavenging assay, apple juice-A, grape juice-A, and pomegranate juice exhibited the highest activity (greater than 90%) while orange juice-B, pineapple juice and tomato juice showed the weakest. The tyrosinase inhibitory effect was highest in apple juice-A, followed by orange juice-A and blueberry juice. The reducing power was highest in pomegranate juice and lowest in pineapple juice and grape juice-A. The antioxidant activity by reducing power was highly correlated with the total polyphenol content.

The Antioxidant, Alcohol Metabolizing Enzyme, and Hepatoprotective Activities of Dendropanax morbifera Vinegar with Traditional Fermentation Methods (전통적인 발효 방법으로 제조한 황칠 식초의 항산화, 알코올 대사 효소 및 간보호 활성)

  • Jung, Kyung Im;Jung, Han Nah;Choi, Young Ju
    • Journal of Life Science
    • /
    • v.32 no.4
    • /
    • pp.290-297
    • /
    • 2022
  • This study was performed to investigate the organic acids, alcohol metabolism enzyme, and antioxidative, nitrite-scavenging, and hepatoprotective effects of Dendropanax morbifera vinegar prepared by a traditional fermentation method. Among the organic acids detected, acetic acid was the highest found, at 91.72 mg/ml, followed by lactic acid (7.31 mg/ml), malic acid (1.36 mg/ml), and succinic acid (1.20 mg/ml). The total polyphenol content of the D. morbifera vinegar was 13.73 ㎍ tannic acid equivalent (TAE)/ml. The 1,1-Diphenyl-2-picrylhydrazy (DPPH) radical scavenging activity of D. morbifera vinegar was 76.04% at a 60% concentration. The superoxide dismutase (SOD) activity of D. morbifera vinegar was increased in a dose-dependent manner, which was 95.14% at a 60% concentration, while the α-glucosidase inhibitory activity of D. morbifera vinegar was 98.94% at a 10% concentration. The effects of D. morbifera vinegar on alcohol metabolism were determined by measuring the generation of reduced nicotinamide adenine dinucleotide (NADH) by alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase (ALDH). The ADH and ALDH activities of D. morbifera vinegar were increased in a dose-dependent manner, 43.62% and 60.39% at a 60% concentration, respectively. The D. morbifera vinegar showed significant protective effects against tacrine-induced cytotoxicity in HepG2 cells at the 0.6% concentration. These results suggest that D. morbifera vinegar has great potential as a resource for high quality functional health beverages.

Antioxidant Activity of Hwangki and Beni-Koji Extracts and Mixture (황기와 홍국추출물 및 혼합물의 항산화 활성)

  • Kim, Jae-Won;Kim, Soon-Dong;Youn, Kwang-Sup
    • Journal of the Korean Society of Food Science and Nutrition
    • /
    • v.40 no.1
    • /
    • pp.1-6
    • /
    • 2011
  • This study was conducted to evaluate the antioxidant activity of liquid beni-koji (LBK), 70% ethanol extracts of beni-koji (EEB) and water extract of Hwangki (WEH). The yields of freeze dried powder of LBK, EEB and WEH were 32.17 g/L, 23.61 g/kg and 196.33 g/kg, respectively. Electron donating ability at 1% (w/v) of LBK, EEB and WEH were 82.67%, 15.71% and 8.60%; reducing power (OD700) were 2.06, 1.64 and 0.45, respectively. SOD-like activities were 24.32%, 11.11%, and 17.94%; nitrite scavenging activities were 74.92%, 72.31% and 31.83%, respectively. TBARS (%) were in order of LBK (69.65%)> EEB (67.32%)> WEH (4.42%). Electron donating ability at 1% (w/v) of EEB : WEH (1:1, w/w. EW), LBK : WEH (1:1, w/w. LW), EEB : LBK: WEH (1:1:1, w/w. ELW) were 14.58%, 60.66% and 20.42%; reducing power ($OD_{700}$) were 1.06, 2.01 and 1.71; SOD-like activities were 18.50%, 26.94% and 18.25%, respectively. While nitrite scavenging activities and TBARS (%) of ELW was higher than those of other materials. Total polyphenol content of LBK, EEB, WEH, EW, LW, ELW were 3.98%, 3.61%, 3.02%, 3.23%, 3.46% and 3.38%; total flavonoid content were 0.89%, 3.91%, 0.30%, 2.59%, 0.46% and 2.33%, respectively. In conclusion, this study provides experimental evidence that mixture of LBK, EEB and WEH could be used as a source of antioxidant ingredients in the food industry.

Phenolic contents and physiological properties of Pleurotus ostreatus by drying method and 30% fermented ethanol extraction for different periods (느타리버섯류의 자실체 건조 방법 및 주정 추출 기간별 페놀성 성분 함량 및 생리활성 효능 비교)

  • Yeob, So-Jin;Park, Hye-Sung;Kang, Suk-Min;Han, Jae-Gu;Lee, Kang-Hyo;Cho, Jae-Han
    • Journal of Mushroom
    • /
    • v.14 no.4
    • /
    • pp.215-219
    • /
    • 2016
  • The total polyphenol and physiological activities of Pleurotus ostreatus 30% fermented ethanol using different drying methods and extraction periods were investigated. Based on the observed polyphenol content and physiological activity, freeze-drying showed better results than hot air-drying method for P. ostreatus extracted with 30% fermented ethanol for more than 15 days. The total phenolic compound content of 'Gosol' following thefreeze-drying method for 15 days showed the highest value of $0.49{\pm}0.02mg/mL$. Freeze-drying with extraction for 30 days for ASI 2344 showed the highest antioxidant activity based on the DPPH radical scavenging rate of $35.50{\pm}3.29%$. Freeze-drying 'Gosol' for 30 days resulted inthe highest anti-inflammatory and nitrite scavenging activity of $48.40{\pm}3.38%$. Our results showed that P. ostreatus is a functional food.

Polyphenol Compound Contents and Physiological Activities in Various Extracts of the Vitex rotundifolia Stems (순비기나무(Vitex rotundifolia) 줄기 추출물의 폴리페놀 함량과 생리활성)

  • Joo, Eun-Young;Lee, Yang-Suk;Kim, Nam-Woo
    • Journal of the Korean Society of Food Science and Nutrition
    • /
    • v.36 no.7
    • /
    • pp.813-818
    • /
    • 2007
  • For this study, extracts of Vitex rotundifolia stems were prepared using reflux water extraction (WE), reflux ethanol extraction (EE) and hot water extract under high pressure (HWE). The extracts were investigated for the total content of phenolic compounds, antioxidant activities, and inhibitory potencies for xanthine oxidase and tyrosinase. The EE extraction method yielded the highest content of polyphenol compounds (176.34 mg/g). The electron donating abilities (EDA) were 93.46${\sim}$96.92%, when extracts were assayed at 1.0 mg/mL. The superoxide dismutase (SOD)-like activity was the highest in the WE extract (47.32% at 1.0 mg/mL). The nitrite scavenging abilities (pH 1.2) were 84.61${\sim}$88.36% and the inhibition of xanthine oxidase were over 90% at 0.5 mg/mL. Tyrosinase inhibition of HWE and WE were 57.84% and 53.47% respectively. It implies that V. rotundifolia stems have potent physiological activities and their activities were differently exhibited depending on solvent fractions.

Antioxidant and Antimicrobial Activities of Green Tea at Different Harvest Time (채취 시기가 다른 녹차의 생리활성 연구)

  • Kim, Bum-Keun;Park, Chan-Eun;Park, Kee-Jai;Lim, Jeong-Ho;Jeong, Jin-Woong;Jeong, Seung-Won;Cho, Chang-Won
    • Journal of the East Asian Society of Dietary Life
    • /
    • v.19 no.4
    • /
    • pp.570-578
    • /
    • 2009
  • The present study was conducted to investigate the antioxidant and antimicrobial activities of green tea at different harvest time. The leaves were collected in late March(Ilro), early April(Okro and Ujeon), late April(Sejak), and early May(Eoksu and Hanra). The total polyphenol content of Sejak was highest (28.87mg TAE/g). Electron donating abilities toward $\alpha$,$\alpha$-diphenyl-$\beta$-picyryl hydrazyl (DPPH) radical were approximately 80%. SOD-like activities were above 30%, where ujeon showed the highest activity ($38.95{\pm}0.96%$). The nitrite scavenging ability was pH-dependent and shown to be highest at pH 1.2, and lowest at pH 6.0. The inhibitory effects against the angiotensin I converting enzyme were over 85%, except for Okro ($58.22{\pm}4.66%$) and Hanra ($77.96{\pm}3.83%$). The tyrosinase inhibition rate increased with harvest time. Okro showed the highest caffeine content ($3.86{\pm}0.32%$) and had the highest antimicrobial activities against Listeria monocytogenes, Staphylococcus aureus, Escherichia coli, and Salmonella typhimurium. The combined results of this work revealed that the antioxidant and antimicrobial activities of green tea were independent of harvest time.

  • PDF

Nutritional contents and physiological activity of Pleurotus eryngii by extraction solvents (추출용매에 따른 큰느타리 버섯의 영양성분 및 생리활성)

  • Park, Hye-Sung;Kim, Su-Yeon;Kim, Hui-Sun;Han, Jae-Gu;Lee, Kang-Hyo;Cho, Jae-Han
    • Journal of Mushroom
    • /
    • v.13 no.4
    • /
    • pp.282-287
    • /
    • 2015
  • Physiological activities of 70% methanol, fermented ethanol and hot-water extracts of Pleurotus eryngii were investigated. Free radical scavenging activities of P. eryngii extracts were determined according to the elimination of DPPH radicals. Their nitrite scavenging activity and total polyphenol content were also determined. Amino acid analysis showed that phenylalanine (Phe) and glutamic acid (Glu) are most abundant essential and non-essential amino acids in the analyzed extracts. The hot-water extract of ASI 2394 represented the highest antioxidant activity with the DPPH radical scavenging rate value of $40.97{\pm}1.65%$. ASI 2820 displayed the superior capacity to eliminate nitrate regardless of extraction solvents. The hot-water extract of ASI 2887 had the highest content of polyphenol. Our results showed that P. eryngii is well qualified as a functional food.

Mineral Content and Antioxidants Activity of Portulaca oleracea (쇠비름의 무기물 함량 및 항산화 활성)

  • Kim, Mi-Joo;Lee, Soo-Jung;Kim, Ra-Jeong;Jeong, Bo-Young;Sung, Nak-Ju
    • Journal of Life Science
    • /
    • v.21 no.10
    • /
    • pp.1393-1400
    • /
    • 2011
  • We investigated the mineral content and antioxidant activity of Portulaca oleracea for biological properties. Total mineral content was 6025.80 mg/100 g, and potassium was the highest at 3846.99 mg/100 g. Water and 80% ethanol extract yields were 14.84% and 24.93%, respectively. The contents of phenolic compounds and flavonoids of ethanol extract were 58.16 mg/g and 20.08 mg/g, and were significantly higher than that of its water extract (49.09 mg/g and 14.98 mg/g, respecitvely). Antioxidant activities and nitrite scavenging activity was significantly elevated in a dose-dependent manner, and that of ethanol extract was higher than that of the water extract. ${\alpha}$-Glucosidase inhibition activity of ethanol extract was significantly higher than the water extract as well. We suggest that the biological properties of Portulaca oleracea are due to its mineral and phenolic contents.