• 대한수의학회지
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• 제46권4호
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• pp.305-313
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• 2006

The primary goal of the wound healing is rapid wound closure. Recent advances in cellular and molecular biology have greatly expanded our understanding of the biologic processes involved in wound repair and tissue regeneration. This study was conducted to develop a new sponge type of biomaterial to be used for either wound dressing or scaffold for tissue engineering. We designed to make a comparative study of the wound healing effect of silk fibroin/hyaluronic acid (SF/HA) blend sponge in full-thickness dermal injury model of rat. Two full-thickness excisions were made on the back of the experimental animals. The excised wound was covered with either the silk fibroin (SF), hyaluronic acid (HA) or SF/HA (7 : 3 or 5 : 5 ratio) blend sponge. On the postoperative days of 3, 7, 10 and 14, the wound area was calculated by image analysis software. Simultaneously, the tissues were stained with Hematoxylin-Eosin and Masson's trichrome methods to measure the area of regenerated epithelium and collagen deposition. In addition, we evaluated the degree of the epithelial cell proliferation using immunohistochemistry for proliferating cell nuclear antigen (PCNA). We found that the half healing time ($HT_{50}$) of SF/HA blend sponge treated groups were significantly decreased as compared with either those of SF or HA treatment group. Furthermore, SF/HA blend sponges significantly increased the size of epithelialization and collagen deposition as well as the number of PCNA positive cells on epidermal basement membrane as compared with those of control treatment. Especially, the 5 : 5 ratio group of SF/HA among all treatment groups was most effective on wound healing rate and histological studies. These results suggest that SF/HA blend sponges could accelerate the wound healing process through the increase of epithelialization, collagen deposition and basal cell proliferation in full thickness skin injury.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제38권4호
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• pp.221-230
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• 2012

Objectives: This study sought to evaluate the efficacy of collagen graft materials, as compared to other graft materials, for use in healing calvarial defects in rabbits. Materials and Methods: Ten mm diameter calvarial defects were made in ten rabbits. The rabbits were then divided into 4 groups: control, autogenous bone graft, SureOss graft, and Teruplug graft. Bone regeneration was evaluated using histological and radiographic methods. Results: Based on visual examination, no distinct healing profile was observed. At 4 weeks after treatment, histological analysis showed there was no bone regeneration in the control group; however, at 8 weeks after treatment, new bone formation was observed around the margin of the defective sites. In the autogenous bone graft group, new bone formation was observed at 4 weeks after treatment and mature bone was detected around the grafted bone after 8 weeks. In the SureOss graft group, at 4 weeks after treatment, acute inflammatory and multinuclear cells were noted around the grafted materials; at 8 weeks after treatment, a decrease in graft materials coupled with new bone formation were observed at the defective sites. In the Teruplug graft group, new bone formation was detected surrounding the bone margin and without signs of inflammation. There were statistically significant differences observed between the graft and control group in terms of bone density as evidenced by radiographic analysis using computed tomography (P<0.05), particularly for the autogenous bone graft group (P<0.001). Conclusion: These results suggested that autogenous bone, SureOss and Teruplug have the ability to induce bone regeneration as compared to an untreated control group. The osteogenic potential of Teruplug was observed to be lower than that of autogenous bone, but similar to that of SureOss.

• Journal of Species Research
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• 제5권1호
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• pp.31-48
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• 2016

Twelve new species of two genera Smenospongia and Cacospongia (Demospongia: Dictyoceratida: Thorectidae) are described from Gageodo Island and Ulleungdo Island, Korea. Of these, ten new species of the Smenospongia are distinguished from the other nine reported species of the genus by the skeletal structure and sponge colour. Primary fibres of the genus Smenospongia are mostly dense, dark colour, and invisible inside of fibres, but they are mostly cored detritus and echinated with spicules. Especially, the end of primary fibres at the surface shows densely cored with spicules. All these new species changed the colour. Two new species of the genus Cacospongia are compared with nine other reported species. In skeletal structure, primary fibres of the genus Cacospongia are light colour, visible inside and more heavily cored with spicules and sands. The skeleton of this genus has large meshes. The colour of the genus Cacospongia does not change.

• Animal cells and systems
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• 제8권4호
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• pp.247-250
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• 2004

A taxonomic study on marine sponges was conducted by SCUBA diving at Gageodo Island, Korea from August 1999 to July 2001. Among them, Haliclona (Gellius) coreana n. sp. turned out to be new to science. This new species seems to be close to H. (G.) arcoferus Vosmaer, 1885, based on the type of spicules. However, it is different in size of toxa and growth form. Toxa of the new species is half in length compared to H. (G.) arcoferus. The growth form is jar shaped in this new species, but flat in H. (G.) arcoferus.

• 한국해양바이오학회지
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• 제1권4호
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• pp.243-251
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• 2006

Dideoxypetrosynol A, a polyacetylene from the marine sponge Petrosia sp., is known to exhibit significant selective cytotoxic activity against a small panel of human tumor cell lines, however, the mechanisms of which are poorly understood. In the present study, it was investigated the further possible mechanisms by which dideoxytetrosynol A exerts its anti-proliferative action in cultured human leukemia cell line U937. We observed that the proliferation-inhibitory effect of dideoxypetrosynol A was due to the induction of G1 arrest of the cell cycle and apoptosis, which effects were associated with up-regulation of cyclin D1 and down-regulation of cyclin E without any change in cyclin-dependent-kinases (Cdks) expression. Dideoxypetrosynol A markedly induced the levels of Cdk inhibitor p16/INK4a expression. Furthermore, down-regulation of phosphorylation of retinoblastoma protein (pRB) by this compound was associated with enhanced binding of pRB and the transcription factor E2F-1. The increase in apoptosis was associated with a dose-dependent up-regulation in pro-apoptotic Bax expression and activation of caspase-3 and caspase-9. Dideoxytetrosynol A decreased the levels of cyclooxygenase (COX)-2 mRNA and protein expression without significant changes in the levels of COX-1, which was correlated with a decrease in prostaglandin E2 (PGE2) synthesis. Furthermore, dideoxytetrosynol A treatment markedly inhibited the activity of telomerase, and the expression of human telomerase reverse transcriptase (hTERT), a main determinant of the telomerase enzymatic activity, was progressively down-regulated by dideoxytetrosynol A treatment in a dose-dependent fashion. Taken together, these findings provide important new insights into the possible molecular mechanisms of the anti-cancer activity of dideoxytetrosynol A.

• 한국세라믹학회지
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• 제45권4호
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• pp.245-249
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• 2008

The SiC and ZrC are critical and essential materials in TRISO coated fuel particles since they act as protective layers against diffusion of metallic and gaseous fission products and provides mechanical strength for the fuel particle. However, SiC and ZrC have critical disadvantage that SiC loses chemical integrity by thermal dissociation at high temperature and mechanical properties of ZrC are weaker than SiC. In order to complement these problems, we made new combinations of the coating layers that the ZrC layers composed of SiC. In this study, after Silicon carbide(SiC) were chemically vapor deposited on graphite substrate, Zirconium carbide(ZrC) were deposited on SiC/graphite substrate by using Zr reaction technology with Zr sponge materials. The different morphologies of sub-deposited SiC layers were correlated with microstructure, chemical composition and mechanical properties of deposited ZrC films. Relationships between deposition pressure and microstructure of deposited ZrC films were discussed. The deposited ZrC films on SiC of faceted structure with smaller grain size has better mechanical properties than deposited ZrC on another structure due to surface growth trend and microstructure of sub-deposited layer.

• Animal Systematics, Evolution and Diversity
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• 제25권2호
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• pp.179-181
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• 2009

A new species of the genus Halichondria (Eumastia) maraensis n. sp. (Demospongiae: Halichondrida: Halichondriidae) was collected from West Marado Island, Daejeong-eup, Seogwipo-si, Jeju-do Island, Korea during the period of Dec. 2006 to Feb. 2007 by a fishing net (60-80 m in depth). Halichondria (E.) maraensis n. sp. is similar to H. (E.) sitiens (Schmidt, 1870) in the type of the spicules. However they differ in spicules size and conical fistules at the surface. Oxeas of the new species are smaller and thicker than those of H. (E.) sitiens. Surface conules of the new species is not transparent, and has no oscules at the terminal short conules but H. (E.) sitiens has hollow papillae and terminal oscules.

• Animal Systematics, Evolution and Diversity
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• 제25권2호
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• pp.141-144
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• 2009

A new marine sponge in the family Ancorinidae, Penares hongdoensis n. sp. was collected from Hongdo Island, Korea by SCUBA diving in 2004. P. hongdoensis n. sp. is closely related to P. cortius de Laubenfels, 1930 in their spicules. However, they differ in kind and size of spicules and growth form. The dichotriaene rhabds of new species are larger than that of P. cortius. And the new species has oxyaster, but P. cortius has oxyspheraster. Although the growth forms of both species are irregular massive, this new species shows conspicuous long projection toward upper part.

• Animal Systematics, Evolution and Diversity
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• 제34권2호
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• pp.79-91
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• 2018

Three new genera and species of siphonostomatoid copepods are described as associates of sponges from shallow water of Samar Island in the Philippines: Samarus filipes n. gen., n. sp., Paurocheres dentatus n. gen., n. sp., and Platymyzon umbonatum n. gen., n. sp. A new family Samarusidae is proposed to accommodate Samarus n. gen. which has rudimentary legs 1-5 represented only by filiform setae. Paurocheres n. gen. is characterized by 2-segmented endopod of leg 4 and reduced setation of legs, and Platymyzon n. gen. by missing of mandibular gnathobase.

• Animal Systematics, Evolution and Diversity
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• 제35권4호
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• pp.200-203
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• 2019

A new marine sponges, Mycale (Carmia) ieoensis n. sp., of the family Mycalidae was collected SCUBA diving in June 2017 from Ieodo Ocean Research Station, Korea. M. (C.) ieoensis n. sp. is encrusting to irregular massive type, yellow in life. This new species is similar to M. nullarosette Hoshino, 1981 in color and growth form but it differs in spicule size and sigma shape. Spicule size of M. (C.) ieoensis n. sp. smaller than that M. nullarosette. Also, M. (C.) ieoensis n. sp. has two size toxa, but M. nullarosette is not. The new species are compared to other Mycale species from the Korean region, and similar species from elsewhere.