• Korean Journal of Plant Tissue Culture
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• v.22 no.5
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• pp.273-276
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• 1995

In vitro propagation of Neoregeria carorinae 'Tricolor' was achieved by using immature flowers and lateral buds, and the plantlets from tissue culture were transplanted and cultivated in greenhouse. The picking times of explants to decrease disappearance of stripes, and in vivo the growth and flowering of regenerated plantlets as influenced by in vivo healed nun were investigated. The normal plantlet were obtained at a frequency of 67%, in the culture of immature flowers picked at 4 weeks after flower bud differentiation, while all leaf stripes disappeared in the culture of immature flowers picked 1 and 5 weeks after flower bud differentiation. In vivo growth of plantlet from immature flower buds was better than those from lateral buds, and the flowering of 27.8% showed in the greenhouse culture of plantlet from immature culture, but the plantlets from lateral buds did not flower at all. The plantlets rooted on the medium with 0.5 mg/L IBA were the most favorable in green house culture, and the kinds and concentrations of auxin in vitro did not have any influence on variation of plane cultured in greenhouse.

• Korean Journal of Plant Tissue Culture
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• v.22 no.4
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• pp.223-227
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• 1995

Immature flowers and lateral buds of Neoregeria carorinae cv. Tricolor were cultured for micropropagation and the collecting times of materials, growth regulators and theirs concentrations, and cultural methods on the formation of adventitious buds and growth were investigated in this experiment The formation rate was the highest in immature flowers collected at 4weeks after flower bud differentiation and in buds at 7weeks after flower differentiation of adventitious buds. MS medium supplemented with 1.0 mg/L BA and 0.5 mg/L BA was the most favorable for the formation of adventitious buds. Solid medium was more effective for the formation of adventitious buds than liquid one. MS medium with 1.0 mg/L NAA was the most suitable for the rooting of regenerated shoot. Liquid medium was effective for the rooting of regenerated shoot than solid one.