• Title/Summary/Keyword: nematodes

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A New Technique for Single Spore Isolation of Two Predacious Fungi Forming Constricting Ring

  • Singh, Kalika Prasad;Kumar, Dharmendra;Bandyopadhyay, Pinaki
    • Mycobiology
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    • v.32 no.4
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    • pp.197-198
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    • 2004
  • A new technique for single spore isolation was developed for predacious fungi forming constricting rings directly on the spores using Dactylaria brochopaga and Arthrobotrys dactyloides. Constricting rings were induced directly on the spores by transferring the spores in 25 ppm solution of DL-Valine in sterile distilled water. Freshly hatched and thoroughly washed second stage juveniles of Meloidogyne incognita were transferred into cavity blocks containing induced rings for trapping and killing of nematodes. The killed nematodes were surface sterilized with streptomycin and inoculated into petri dishes containing maize meal agar media with 100 ppm streptomycin. The petri dishes were incubated at $29{\pm}1^{\circ}C$ for few days which yielded axenic culture of these fungi.

Pathogenicity of Entomopathogenic Nematodes to Some Agro-Forest Insect Pests (농림해충에 대한 곤충병원성 선충의 병원성)

  • 이상명;이동운;추호렬;김도완;김준범
    • The Korean Journal of Soil Zoology
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    • v.2 no.2
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    • pp.76-82
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    • 1997
  • Mortality of some agro-forest insect pests, Agrotis segetum, Blitopertha orientalis, Agerastica coerulea, Glyphodes perspectalis and Acantholyda parki caused by entomopathogenic nematodes was investigated in laboratory and in pot. Steinernema carpocapsae All and Pocheon strain were effective against 2nd of 3rd instar larvae of A. segetum showing 100% mortalities at the concentration of 10Ijs/larva but not effective against 4th inster larvae. Mortality of B. orientalis was 100% after 26 days in H. bacteriopora NC 1, H. bacteriophora Hamyang and S. glageri NC strain treatment. S. monticulum also showed 100% mortalities against A. coerulea andd G. perspectalis at the concentration of 80 and 40 Ijs/larva. However, H. bacteriophora Cheju and S. glaseri Cheju strain were not effective against A. parki, i.e., showing 23.3 and 20.0% mortalities, respectively at the concentration of 160Ijs/larva. S. glaseri Hanrim strain was more effective than H. bacteriophora Cheju strain against Pryeria sinica.

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Soil Nematode Fauna in Dokdo Island of Korea (독도의 토양 선충상)

  • Kim, Dong-Geun;Park, Byung-Yong;Ryu, Young-Hyun
    • Research in Plant Disease
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    • v.18 no.4
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    • pp.381-386
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    • 2012
  • Nematode fauna in Dokdo island was investigated for two years between 2008 and 2009. Population density and biomass were $239,500m^2$ (26,000-836,000) and $103.9mg/m^2$ (3.0-388.6), respectively. Total of 31 species of nematodes were identified : Acrobeloides is the most important genus with prominence value (PV) of 117.8, followed by Rhabditidae (PV = 81.9), Prismatolaimus (PV = 39.0), and Aphelenchoides (PV = 31.4). Among feeding group, bacteriovorus nematode composed 77% of density and 62% of biomass followed by plant-parasitic and fungivorus nematodes. A species of Pratylenchus having distinctive tail terminus is under study as a new species. Biological indices, Shannon and Wiener index (2.46), Simpson's diversity index (0.86), Species evenness (0.73), Species richness (1.30) indicate that nematode fauna in Dokdo island is diverse and unique. This study may give a general idea of nematode fauna at the Dokdo island.

Damage and Occurrence of Pratylenchus vulnus and Paratylenchus sp. on Spray Chrysanthemum, Dendranthema grandiflourm Kitamura (스프레이국화재배지의 사과뿌리썩이선충과 침선충의 발생 및 피해)

  • Lee, Jae-Kook;Park, Byeong-Yong;Choi, Dong-Ro;Heo, Jae-Won
    • Korean journal of applied entomology
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    • v.47 no.4
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    • pp.473-478
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    • 2008
  • Root-lesion nematode (Pratylenchus vulnus) and pin nematode (Paratylenchus sp.) were detected with high population density at the spray chrysanthemum greenhouse in Gumi, Gyeongbuk. The average density of P. vulnus and Paratylenchus sp. was 667 and 716 nematodes per 100 g soil and P. vulnus density were distributed 87% to the depth of $0{\sim}30$ cm in greenhouse. When spray chrysanthemum cv Chopin, was transplanted in September 2004 in greenhouse, P. vulnus were 854 nematodes/100 g soil in planting and increased 14,985 nematodes/100 g soils and 1g root after 10 weeks in harvest. Shoot weight and shoot height of spray chrysanthemum decreased 24.8% and 8.0% compared with non-infested P. vulnus after 10 weeks in greenhouse.