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(Image Analysis of Electrophoresis Gels by using Region Growing with Multiple Peaks) (다중 피크의 영역 성장 기법에 의한 전기영동 젤의 영상 분석)

  • 김영원;전병환
    • Journal of KIISE:Software and Applications
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    • v.30 no.5_6
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    • pp.444-453
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    • 2003
  • Recently, a great interest of bio-technology(BT) is concentrated and the image analysis technique for electrophoresis gels is highly requested to analyze genetic information or to look for some new bio-activation materials. For this purpose, the location and quantity of each band in a lane should be measured. In most of existing techniques, the approach of peak searching in a profile of a lane is used. But this peak is improper as the representative of a band, because its location does not correspond to that of the brightest pixel or the center of gravity. Also, it is improper to measure band quantity in most of these approaches because various enhancement processes are commonly applied to original images to extract peaks easily. In this paper, we adopt an approach to measure accumulated brightness as a band quantity in each band region, which Is extracted by not using any process of changing relative brightness, and the gravity center of the region is calculated as a band location. Actually, we first extract lanes with an entropy-based threshold calculated on a gel-image histogram. And then, three other methods are proposed and applied to extract bands. In the MER method, peaks and valleys are searched on a vertical search line by which each lane is bisected. And the minimum enclosing rectangle of each band is set between successive two valleys. On the other hand, in the RG-1 method, each band is extracted by using region growing with a peak as a seed, separating overlapped neighbor bands. In the RG-2 method, peaks and valleys are searched on two vertical lines by which each lane is trisected, and the left and right peaks nay be paired up if they seem to belong to the same band, and then each band region is grown up with a peak or both peaks if exist. To compare above three methods, we have measured the location and amount of bands. As a result, the average errors in band location of MER, RG-1, and RG-2 were 6%, 3%, and 1%, respectively, when the lane length is normalized to a unit value. And the average errors in band amount were 8%, 5%, and 2%, respectively, when the sum of band amount is normalized to a unit value. In conclusion, RG-2 was shown to be more reliable in the accuracy of measuring the location and amount of bands.

Development of Regularized Expectation Maximization Algorithms for Fan-Beam SPECT Data (부채살 SPECT 데이터를 위한 정칙화된 기댓값 최대화 재구성기법 개발)

  • Kim, Soo-Mee;Lee, Jae-Sung;Lee, Soo-Jin;Kim, Kyeong-Min;Lee, Dong-Soo
    • The Korean Journal of Nuclear Medicine
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    • v.39 no.6
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    • pp.464-472
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    • 2005
  • Purpose: SPECT using a fan-beam collimator improves spatial resolution and sensitivity. For the reconstruction from fan-beam projections, it is necessary to implement direct fan-beam reconstruction methods without transforming the data into the parallel geometry. In this study, various fan-beam reconstruction algorithms were implemented and their performances were compared. Materials and Methods: The projector for fan-beam SPECT was implemented using a ray-tracing method. The direct reconstruction algorithms implemented for fan-beam projection data were FBP (filtered backprojection), EM (expectation maximization), OS-EM (ordered subsets EM) and MAP-EM OSL (maximum a posteriori EM using the one-step late method) with membrane and thin-plate models as priors. For comparison, the fan-beam protection data were also rebinned into the parallel data using various interpolation methods, such as the nearest neighbor, bilinear and bicubic interpolations, and reconstructed using the conventional EM algorithm for parallel data. Noiseless and noisy projection data from the digital Hoffman brain and Shepp/Logan phantoms were reconstructed using the above algorithms. The reconstructed images were compared in terms of a percent error metric. Results: for the fan-beam data with Poisson noise, the MAP-EM OSL algorithm with the thin-plate prior showed the best result in both percent error and stability. Bilinear interpolation was the most effective method for rebinning from the fan-beam to parallel geometry when the accuracy and computation load were considered. Direct fan-beam EM reconstructions were more accurate than the standard EM reconstructions obtained from rebinned parallel data. Conclusion: Direct fan-beam reconstruction algorithms were implemented, which provided significantly improved reconstructions.

Cloning, cSNP Identification, and Genotyping of Pig Complement Factor B(CFB) Gene Located on the SLA Class III Region (SLA Class III 영역의 돼지 Complement Factor B(CFB) 유전자의 Cloning, cSNP 동정 및 유전자형 분석)

  • Kim, Jae-Hwan;Lim, Hyun-Tae;Seo, Bo-Yeong;Zhong, Tao;Yoo, Chae-Kyoung;Jung, Eun-Ji;Jeon, Jin-Tae
    • Journal of Animal Science and Technology
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    • v.50 no.6
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    • pp.753-762
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    • 2008
  • The primers for RT-PCR and RACE-PCR were designed by aligning the pig genomic sequence and the human complement factor B(CFB) coding sequence(CDS) from the GenBank. Each PCR product was amplified in pig cDNA and sequencing was carried out. The CDS length of pig CFB gene was determined to be 2298 bp. In addition, the pig CDS was more longer than human and mouse orthologs because of insertion and deletion. The identities of porcine nucleotide sequences with those of human and mice were 84% and 80%, and the identities of amino acids were 79% to 77%, respectively. Three complement control protein(CCP) domains, one Von Willebrand factor A(VWFA) domain and a serine protease domain, that are revealed typically in mammals, were found in the pig CFB gene. Based on the CDSs determined, the primers were designed in intron regions for amplification of entire length of exons. In amplification and direct sequencing with genomic DNAs of six pig breeds, three cSNPs(coding single nucleotide polymorphisms) were identified and verified as missense mutations. Using the Multiplex-ARMS method, we genotyped and verified the mutations identified from direct sequencing. To demonstrate recrudescence, we performed both direct sequencing and Multiplex-ARMS with two randomly selected DNA samples. The genotype of each sample exhibited the same results using both methods. Therefore, three cSNPs were identified from pig CFB gene and that can be used for haplotype analysis of the swine leukocyte antigen(SLA) class III region. Moreover, the results indicate that the Multiplex-ARMS method should be powerful for genotyping of genes in the SLA region.

A Literature Review and Classification of Recommender Systems on Academic Journals (추천시스템관련 학술논문 분석 및 분류)

  • Park, Deuk-Hee;Kim, Hyea-Kyeong;Choi, Il-Young;Kim, Jae-Kyeong
    • Journal of Intelligence and Information Systems
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    • v.17 no.1
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    • pp.139-152
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    • 2011
  • Recommender systems have become an important research field since the emergence of the first paper on collaborative filtering in the mid-1990s. In general, recommender systems are defined as the supporting systems which help users to find information, products, or services (such as books, movies, music, digital products, web sites, and TV programs) by aggregating and analyzing suggestions from other users, which mean reviews from various authorities, and user attributes. However, as academic researches on recommender systems have increased significantly over the last ten years, more researches are required to be applicable in the real world situation. Because research field on recommender systems is still wide and less mature than other research fields. Accordingly, the existing articles on recommender systems need to be reviewed toward the next generation of recommender systems. However, it would be not easy to confine the recommender system researches to specific disciplines, considering the nature of the recommender system researches. So, we reviewed all articles on recommender systems from 37 journals which were published from 2001 to 2010. The 37 journals are selected from top 125 journals of the MIS Journal Rankings. Also, the literature search was based on the descriptors "Recommender system", "Recommendation system", "Personalization system", "Collaborative filtering" and "Contents filtering". The full text of each article was reviewed to eliminate the article that was not actually related to recommender systems. Many of articles were excluded because the articles such as Conference papers, master's and doctoral dissertations, textbook, unpublished working papers, non-English publication papers and news were unfit for our research. We classified articles by year of publication, journals, recommendation fields, and data mining techniques. The recommendation fields and data mining techniques of 187 articles are reviewed and classified into eight recommendation fields (book, document, image, movie, music, shopping, TV program, and others) and eight data mining techniques (association rule, clustering, decision tree, k-nearest neighbor, link analysis, neural network, regression, and other heuristic methods). The results represented in this paper have several significant implications. First, based on previous publication rates, the interest in the recommender system related research will grow significantly in the future. Second, 49 articles are related to movie recommendation whereas image and TV program recommendation are identified in only 6 articles. This result has been caused by the easy use of MovieLens data set. So, it is necessary to prepare data set of other fields. Third, recently social network analysis has been used in the various applications. However studies on recommender systems using social network analysis are deficient. Henceforth, we expect that new recommendation approaches using social network analysis will be developed in the recommender systems. So, it will be an interesting and further research area to evaluate the recommendation system researches using social method analysis. This result provides trend of recommender system researches by examining the published literature, and provides practitioners and researchers with insight and future direction on recommender systems. We hope that this research helps anyone who is interested in recommender systems research to gain insight for future research.

Molecular Phylogenetic Study of Nesiohelix samarangae Based on Metallothionein Gene (동양달팽이(Nesiohelix samarangae)의 metallothionein 유전자를 기초로 한 분자계통 분류학적 연구)

  • Lee, Jun-Seo;Min, Byung-Jun;Kang, Se-Won;Lee, Jae-Bong;Baek, Moon-Ki;Hwang, Seung-Young;Kim, So-Hee;Kho, Weong-Gyu;Choi, Sang-Haeng;Chae, Sung-Hwa;Park, Hong-Seog;Han, Yeon-Soo;Lee, Jun-Sang;Jeong, Kye-Heon;Lee, Yong-Seok
    • The Korean Journal of Malacology
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    • v.24 no.1
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    • pp.73-80
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    • 2008
  • Numerous morphological studies on N. samarangae have been well conducted over the last ten years. In this context, we have attemtped to do molecular phylogenetic analysis by using metallothionein (MT) gene from N. samarangae. To this end, we cloned the full length cDNA of MT from cDNA library of N. samarangae. The complete cDNA sequences were obtained from the expressed sequence tag (EST) sequencing project of N. samarangae, The coding region of 195 bp gives an amino acid sequence of 65 residues including methionine. There are 5' (61 bp) and 3' (48 bp) untranslated region at both ends of the Ns-MT cDNA sequence. The combined results from BLAST analyses, multiple sequence alignment and molecular phylogenetic study of Ns-MT cDNA indicate that N. samarangae has similarity to land snails such as Helix pomatia, Helix aspersa and Arianta arbustorum.

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A Hybrid Forecasting Framework based on Case-based Reasoning and Artificial Neural Network (사례기반 추론기법과 인공신경망을 이용한 서비스 수요예측 프레임워크)

  • Hwang, Yousub
    • Journal of Intelligence and Information Systems
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    • v.18 no.4
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    • pp.43-57
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    • 2012
  • To enhance the competitive advantage in a constantly changing business environment, an enterprise management must make the right decision in many business activities based on both internal and external information. Thus, providing accurate information plays a prominent role in management's decision making. Intuitively, historical data can provide a feasible estimate through the forecasting models. Therefore, if the service department can estimate the service quantity for the next period, the service department can then effectively control the inventory of service related resources such as human, parts, and other facilities. In addition, the production department can make load map for improving its product quality. Therefore, obtaining an accurate service forecast most likely appears to be critical to manufacturing companies. Numerous investigations addressing this problem have generally employed statistical methods, such as regression or autoregressive and moving average simulation. However, these methods are only efficient for data with are seasonal or cyclical. If the data are influenced by the special characteristics of product, they are not feasible. In our research, we propose a forecasting framework that predicts service demand of manufacturing organization by combining Case-based reasoning (CBR) and leveraging an unsupervised artificial neural network based clustering analysis (i.e., Self-Organizing Maps; SOM). We believe that this is one of the first attempts at applying unsupervised artificial neural network-based machine-learning techniques in the service forecasting domain. Our proposed approach has several appealing features : (1) We applied CBR and SOM in a new forecasting domain such as service demand forecasting. (2) We proposed our combined approach between CBR and SOM in order to overcome limitations of traditional statistical forecasting methods and We have developed a service forecasting tool based on the proposed approach using an unsupervised artificial neural network and Case-based reasoning. In this research, we conducted an empirical study on a real digital TV manufacturer (i.e., Company A). In addition, we have empirically evaluated the proposed approach and tool using real sales and service related data from digital TV manufacturer. In our empirical experiments, we intend to explore the performance of our proposed service forecasting framework when compared to the performances predicted by other two service forecasting methods; one is traditional CBR based forecasting model and the other is the existing service forecasting model used by Company A. We ran each service forecasting 144 times; each time, input data were randomly sampled for each service forecasting framework. To evaluate accuracy of forecasting results, we used Mean Absolute Percentage Error (MAPE) as primary performance measure in our experiments. We conducted one-way ANOVA test with the 144 measurements of MAPE for three different service forecasting approaches. For example, the F-ratio of MAPE for three different service forecasting approaches is 67.25 and the p-value is 0.000. This means that the difference between the MAPE of the three different service forecasting approaches is significant at the level of 0.000. Since there is a significant difference among the different service forecasting approaches, we conducted Tukey's HSD post hoc test to determine exactly which means of MAPE are significantly different from which other ones. In terms of MAPE, Tukey's HSD post hoc test grouped the three different service forecasting approaches into three different subsets in the following order: our proposed approach > traditional CBR-based service forecasting approach > the existing forecasting approach used by Company A. Consequently, our empirical experiments show that our proposed approach outperformed the traditional CBR based forecasting model and the existing service forecasting model used by Company A. The rest of this paper is organized as follows. Section 2 provides some research background information such as summary of CBR and SOM. Section 3 presents a hybrid service forecasting framework based on Case-based Reasoning and Self-Organizing Maps, while the empirical evaluation results are summarized in Section 4. Conclusion and future research directions are finally discussed in Section 5.

ALL-SKY OBSERVATION OF THE 2001 LEONID METEOR STORM: 1. METEOR MAGNITUDE DISTRIBUTION (전천 카메라를 이용한 2001 사자자리 유성우 관측: 1. 유성 등급 분포)

  • 김정한;정종균;김용하;원영인;천무영;임홍서
    • Journal of Astronomy and Space Sciences
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    • v.20 no.4
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    • pp.283-298
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    • 2003
  • The 2001 Leonid meteor storm has been observed all over the world, and its most intense flux since the last few decades has caused great interest among both laymen and experts. Especially, its maximum hours occurred at dawn hours of Nov. 19 in the east Asia, during which moonless clear night at the Mt. Bohyun allowed us near perfect condition of observation. Observation was carried out in the period of 01:00∼05:40(KST), which include the predicted maximum hours, with all-sky camera installed for upper atmospheric airglow research. Tn this paper we analyze 68 all-sky images obtained in this period, which contain records of 172 meteors. Utilizing the zenith hourly rate(ZHR) of 3000 and magnitude distribution index of 2, which were reported to International Meteor Organization by visible observers in the east Asia, we estimate the limiting magnitude of about 3 for meteors detected in our all-sky images. We then derive magnitudes of 83 meteors with clear pixel brightness outlines among the initially detected 172 meteors by comparing with neighbor standard stars. Angular velocities of meteors needed for computing their passing times over an all-sky image are expressed with a simple formula of an angle between a meteor head and the Leonid radiant point. The derived magnitudes of 83 meteors are in the range of -6∼-1 magnitude, and its distribution shows a maximum new -3mag. The derived magnitudes are much smaller than the limiting magnitude inferred from the comparison with the result of naked-eye observations. The difference may be due to the characteristic difference between nearly instantaneuous naked-eye observations and CCD observations with a long exposure. We redetermine magnitudes of the meteors by adjusting a meteor lasting time to be consistent with the naked-eye observations. The relative distribution of the redetermined magnitudes, which has a maximum at 0 mag., resembles that of the magnitudes determined with the in-principle method. The relative distribution is quite different from ones that decrease monotonically with decreasing magnitudes for meteors(1∼6) sensitive to naked-eye observations. We conclude from the magnitude distribution of our all-sky observation that meteors brighter than about 0 mag., appeared more frequently during the 2001 Leonid maximum hours. The frequent appearance of bright meteors has significantly important implication for meteor research. We noted, however, considerably large uncertainties in magnitudes determined only by comparing standard stars due to the unknown lasting time of meteors and the non-linear sensitivity of all-sky camera.

Development of an Approach for Analysing Vegetation Community Mosaic Using Landscape Metrics (경관지수를 활용한 식생군락 모자이크화 분석법)

  • Lee, Peter Sang-Hoon;Jeong, Jong-Chul
    • Journal of Cadastre & Land InformatiX
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    • v.47 no.1
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    • pp.161-178
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    • 2017
  • Whereas the demand for development of forested areas covering more than 60% of Korean territory, permission on the forest development has been still given from the perspective of effective land utilization rather than conservation. As the assessment of large forested areas usually focuses more on forest structure, it has its limitation of observing and analyzing the interior change in forest in this way. This study was aimed at computing landscape metrics using a presence vegetation map and FRAGTSTATS 4.2 and analyzing vegetation mosaics. Colonies in native vegetation were classified into a series of major groups and sub-groups based on the native species within the colonies. The colonies were investigated by analyzing a suite of landscape metrics - Core Area, Percentage of Landscape, Number of Patches, Patch Density, Largest Patch Index, Total Edge, Edge Density, Landscape Shape Index, Mean Patch Area, Euclidean Nearest Neighbor. In the Chungnam province major groups and sub-groups of colonies classified based on the proportion of pine and oak species, and pine species was the principal one in terms of distribution area. As for the competition between pines and oaks, while the coverage of pine-centered colonies were three times larger than those of oak-centered ones, pine colonies showed the greater number of patches and therefore higher fragmentation than oaks at the major group level. For the sub-groups, the largest coverage colonies were not only indicated by Pinus densiflora-Quesrcus mongolica colonies among P. densiflora-centered colonies, Q. accutissima colonies among Q. accutissima-centered ones, Q. accutissima-P. densiflora colonies among Q. accutissima-centered ones, Q. mongolica colonies among Q. mongolica-centered ones, P. thumbergii colonies among P. thumbergii-centered ones, and Q. serrata-Q. acutissima colonies among Q. serrata-centered ones, but also revealed more severely mosaicked than other smaller colonies. The overall mosaicking degree estimated by landscape metrics was considered useful for monitoring and investigating vegetation. However, in order to develop management strategy based on analyzing the reason for the mosaicking process and anticipating a trend in vegetation succession, it is essential to further study about ecological characteristics of each colony in the vegetation.

Genetic Variation and Polymorphism in Rainbow Trout, Oncorhynchus mykiss Analysed by Amplified Fragment Length Polymorphism

  • Yoon, Jong-Man;Yoo, Jae-Young;Park, Jae-Il
    • Journal of Aquaculture
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    • v.17 no.1
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    • pp.69-80
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    • 2004
  • The objective of the present study was to analyze genetic distances, variation and characteristics of individuals in rainbow trout, Oncorhynchus mykis using amplified fragment length polymorphism (AFLP) method as molecular genetic technique, to detect AFLP band patterns as genetic markers, and to compare the efficiency of agarosegel electrophoresis (AGE) and polyacrylamide gel electrophoresis (PAGE), respectively. Using 9 primer combinations, a total of 141 AFLP bands were produced, 108 bands (82.4%) of which were polymorphic in AGE. In PAGE, a total of 288 bands were detected, and 220 bands (76.4%) were polymorphic. The AFLP fingerprints of AGE were different from those of PAGE. Separation of the fragments with low molecular weight and genetic polymorphisms revealed a distinct pattern in the two gel systems. In the present study, the average bandsharing values of the individuals between two populations apart from the geographic sites in Kangwon-do ranged from 0.084 to 0.738 of AGE and PAGE. The bandsharing values between individuals No.9 and No. 10 showed the highest level within population, whereas the bandsharing values between individuals No.5 and No.7 showed the lowest level. As calculated by bandsharing analysis, an average of genetic difference (mean$\pm$SD) of individuals was approximately 0.590$\pm$0.125 in this population. In AGE, the single linkage dendrogram resulted from two primers (M11+H11 and M13+H11), indicating six genetic groupings composed of group 1 (No.9 and 10), group 2 (No. 1, 4, 5, 7, 10, 11, 16 and 17), group 3 (No. 2, 3, 6, 8, 12, 15 and 16), group 4 (No.9, 14 and 17), group 5 (No. 13, 19, 20 and 21) and group 6 (No. 23). In AGE, the genetic distances among individuals of between-population ranged from 0.108 to 0.392. In AGE, the shortest genetic distance (0.108) displaying significant molecular differences was between individuals No.9 and No. 10. Especially, the genetic distance between individuals No. 23 and the remnants among individuals within population was highest (0.392). Additionally, in the cluster analysis using the PAGE data, the single linkage dendrogram resulted from two primers (M12+H13 and M11+H13), indicating seven genetic groupings composed of group 1 (No. 15), group 2 (No. 14), group 3 (No. 11 and 12), group 4 (No.5, 6, 7, 8, 10 and 13), group 5 (No.1, 2, 3 and 4), group 6 (No.9) and group 7 (No. 16). By comparison with the individuals in PAGE, genetic distance between No. 10 and No. 7 showed the shortest value (0.071), also between No. 16 and No. 14 showed the highest value (0.242). As with the PAGE analysis, genetic differences were certainly apparent with 13 of 16 individuals showing greater than 80% AFLP-based similarity to their closest neighbor. The three individuals (No. 14, No. 15 and No. 16) of rainbow trout between two populations apart from the geographic sites in Kangwon-do formed distinct genetic distances as compared with other individuals. These results indicated that AFLP markers of this fish could be used as genetic information such as species identification, genetic relationship or analysis of genome structure, and selection aids for genetic improvement of economically important traits in fish species.

Research on the Food Habits of Housewives of Korean Students Residing in a Southern Region of Texas, U.S.A. (미국 Texas 일부 지역에 거주하는 한국 유학생 부인들의 식생활습관에 관한 연구)

  • 김은실;송청락;정복미;심영자
    • Culinary science and hospitality research
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    • v.9 no.2
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    • pp.11-21
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    • 2003
  • The purpose of this study was to research on the preparing fermented foods and food habits of Korean women in Texas U. S. A. The preparing method of fermented foods and food habits of Korean housewives whose husbands are studying at the various universities in Houston, Texas are surveyed. The analysed results indicated were as follows ; 1. 64.4% of women were in the thirties at their age, those in twenties 29.7%. 75.2% of subject graduated college. Period of residence in America were 52.5% under 3yr, 25.7% over 5yr. The respondents were aged twenties(29.7%) to thirties(64.4%) who have graduated from college(75.2%) or graduate school(24.8%) and have had the residence period of less than three-(52.5%) to more than five years(25.7%). 2. The kind of cuisine chosen for dinner were Korean style food. The most considerable person when preparing menu were husband. 48.51% of those in age were 3∼39yr(p < 0.1). 60.40% of the response graduated college(p < 0.1). The Korean-style foods overwhelmed over western ones in their frequency for dinner(82.2% vs. 17.8%). The person most considered for choosing menu was their husbands as responded by the majority groups of aged thirties(48.5%), college graduates(60.4%) and residence period of less than three years(42.6%). They satisfied comparatively when they prepared the meal (p < 0.1). 55.4% of the response learned cooking method from neighbor, 39.6% of remainder studied from cuisine book, newspaper, magazine, TV. They satisfied comparatively for the preparation of the meal(56.4%) and have learned the new methods of cooking from neighborhoods(55.4%) or cuisine book, newspaper, magazine and TV(39.6%). 3. The frequency of eating-out mostly had once a month. 30.69% of response were 30∼39yr(p < 0.1). 33.66% of subjects lived under 3yr in America. The frequency of eating-out mostly had once a month. 30.69% of response were 30-39yr(p < 0.1). 33.66% of subjects lived under 3yr in America. Eating-out once a month occupied 30.7, 33.7, and 20.7% of the selected group of aged thirties, college graduates, and residence periods of less than three years, each respectively. The type of food selecting eating-out were western style. 38.31% of them were 30∼39yr(p < 0.1). 39.60% of them graduated college(p < 0.1). 29.70% of them lived under 3yr in America. For eating-out menu, the western-style foods were most favored by the groups of aged thirties(38.3%), college graduates(39.6%), and residence periods of less than three years(29.7%). 4. 50.5% of subject had beef once three days, 23.8% of the response had pork once two weeks, 27.7% of subject ate chicken once two weeks, 34.7% of the response enjoyed fish once a week. Eating animal foods once three days for beef(50.5%), once two weeks for pork(23.8%) and chicken(27.7%), and once a week for fish(34.7%) were most frequent. 5. Korean traditional fermented food were mostly enjoyed in order of kanjang(91.1 %), kochujang(87.2%), fermented fish(85.1%), deonjang(81.2%), jang-a-chi(76.2%). 81.2% of subject used prepared-food by themselves. 56.4% of response made kimch at home. Among Korean traditional fermented foods, kimchi(56.4%) and prepared side dishes(81.2%) were provided at home while kanjang(91.1%), kochujang(87.2%), fermented fish(85.1%), deonjang(81.2%), and jang-a-chi(76.2%) were mostly purchased from stores.

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