• Journal of fish pathology
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• v.3 no.1
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• pp.1-9
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• 1990

The present study was carried out to reveal the characteristics of organism responsible for so called vibriosis prevailing in warm water season among cultured kuruma prawn Penaeus japonicus. A bacterium was isolated from the heart, lymphoid organ and muscle of the diseased kuruma prawn. Six strains obtained from diseased kuruma prawn in some culture farms in Namhae and Anhung from august to october in 1989 were submitted to the morphological, biochemical and physiological characterization. All the strains were gram-negative, nonsporning short rods with one polar flagellum. Glucose was fermented with no gas production by these strains. Some distinguishing features of the organisms were negative to lysine, arginine, ornithine decarboxilization test. In general, the temperature $20{\sim}27^{\circ}C$, the NaCl concentration of 2~3% and pH of 6~8 were optimal for the growth in peptone water. The organisms were sensitive to the vibrio static agent 0/129. The isolated should be identified as Vibrio sp.. No essential differences in histopathological finding were noted between the naturally and the experimentally infected prawns. Most characteristic pathological changes were extensive necrosis caused by severe bacteria invasion and multiple formation of meranized nodules in the lymphoid organ.

• Experimental Neurobiology
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• v.26 no.5
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• pp.241-251
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• 2017

Saturation mutagenesis was performed on a single position in the voltage-sensing domain (VSD) of a genetically encoded voltage indicator (GEVI). The VSD consists of four transmembrane helixes designated S1-S4. The V220 position located near the plasma membrane/extracellular interface had previously been shown to affect the voltage range of the optical signal. Introduction of polar amino acids at this position reduced the voltage-dependent optical signal of the GEVI. Negatively charged amino acids slightly reduced the optical signal by 33 percent while positively charge amino acids at this position reduced the optical signal by 80%. Surprisingly, the range of V220D was similar to that of V220K with shifted optical responses towards negative potentials. In contrast, the V220E mutant mirrored the responses of the V220R mutation suggesting that the length of the side chain plays in role in determining the voltage range of the GEVI. Charged mutations at the 219 position all behaved similarly slightly shifting the optical response to more negative potentials. Charged mutations to the 221 position behaved erratically suggesting interactions with the plasma membrane and/or other amino acids in the VSD. Introduction of bulky amino acids at the V220 position increased the range of the optical response to include hyperpolarizing signals. Combining The V220W mutant with the R217Q mutation resulted in a probe that reduced the depolarizing signal and enhanced the hyperpolarizing signal which may lead to GEVIs that only report neuronal inhibition.

• Ocean and Polar Research
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• v.34 no.4
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• pp.403-411
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• 2012

Among others, a question that has long been unanswered is why the seasonal variation of volume transport is larger in the Soya and Korea/Tsushima Straits than in the Tsugaru Strait. An attempt is made to answer this question in terms of the island rule with friction being taken into account. The problem is idealized as a simple model. The model results indicate that volume transport through a channel is determined not only by the circulation created around the adjacent island but also by those created around the neighboring islands farther away. The latter is due to the presence of bottom friction in the channels. The volume transports through the Korea/Tsushima, Tsugaru and Soya Straits estimated from the model using observed wind data show the general pattern of observed seasonality, although they contain large errors associated with the uncertain frictional parameter employed in the model. The model indicates that the observed seasonality arises essentially from the fact that wind stress curl changes its sign, from negative in the summer to positive in winter, following a large fluctuation of zero-stress curl latitude east of Hokkaido.

• Bulletin of the Korean Chemical Society
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• v.35 no.5
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• pp.1440-1448
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• 2014

Co(II)-tetrasulfonated phthalocyanine (CoTSP) is known to be aggregated to dimer at high concentration levels in water. A study on the aggregation of CoTSP using multivariate curve resolution analysis of the visible absorbance spectra over a concentration range of 30, 40 and 50 ${\mu}M$ in the presence of dimethyl sulfoxide (DMSO), dimethyl formamide (DMF), acetonitrile (AN) and ethanol (EtOH) in the concentration range of 0 to 3.57 M is conducted. A hard modeling-based multivariate curve resolution method was applied to determine the dissociation constants of the CoTSP aggregates at various temperatures ranging from 25, 45 and $65^{\circ}C$ and in the presence of various co-solvents. Dissociation constant for aggregation was increased and then decrease by temperature and concentration of phthalocyanine, respectively. Utilizing the vant Hoff relation, the enthalpy and entropy of the dissociation equilibriums were calculated. For the dissociation of both aggregates, the enthalpy and entropy changes were positive and negative, respectively. Molecular dynamics simulation of cosolvent effect on CoTSP aggregation was done to confirm spectroscopy results. Results of radial distribution function (RDF), root mean square deviation (RMSD) and distance curves confirmed more effect of polar solvent to decrease monomer formation.

• The Korean Journal of Malacology
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• v.30 no.4
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• pp.443-447
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• 2014

Triploids have several potential advantages over diploids in aquaculture, drawing an elevated commercial reaction into the realistic application of the techniques despite we are still in the early stage of triploid industry for the Pacific oysters Crassostrea gigas. We traced the growth performance of the triploid C. gigas for over a year from hatchery spats, which was created by manipulations of chemicals (Cytochalasin B, CB or 6-Dimethylaminopurine, 6-DMAP). The growth was clearly marked by an initial longer dormancy and following a great magnitude of heterogeneity. The dormancy was almost 9 to 10-month long or even longer and was considered as a downside of the creation. The heterogeneity was magnified by appearance of extraordinarily growing oysters in part during summer season, which could be a representative upside of the triploids. Overall, however, the results were not as positive as were expected. The longer dormancy and following heterogeneity observed in our practice could be marked as an additional negative sign of the chemical use. The present study, thus, might be highly indicative in the introduction of biological cross between tetraploid and diploid to produce natural triploid embryos.

• Journal of Microbiology and Biotechnology
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• v.28 no.9
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• pp.1536-1541
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• 2018

A yellowish, flexirubin-pigment-producing strain $I3-3^T$ was isolated from river water in Iksan, the Republic of Korea. The strain was gram-negative, aerobic, non-motile, showed catalase and oxidase activities, and could grow at a temperature range of $10-35^{\circ}C$, pH 5.0-10 and 0-2.0% (w/v) of NaCl. The major fatty acids were iso-$C_{15:0}$, iso-$C_{17:0}$ 3-OH and summed feature 3 (comprising $C_{16:1}{\omega}7c$ and/or $C_{16:1}{\omega}6c$). The isolate contained phosphatidylethanolamine, one aminolipid, and two unidentified lipids as the major polar lipids. Menaquinone-6 (MK6) was the major respiratory quinone. The G+C content of the genomic DNA of strain $I3-3^T$ was 35.6%. Comparison of the 16S rRNA gene sequence with the sequences of the closely related type strains showed highest sequence similarity of 96.95% and 96.93% to Flavobacterium nitrogenifigens $NXU-44^T$ and Flavobacterium compostarboris $15C3^T$, respectively. Based on phenotypic and phylogenetic distinctiveness, strain $I3-3^T$ is considered as a member of novel species within the genus Flavobacterium, for which Flavobacterium amnigenum sp. nov. is proposed. The type strain is $I3-3^T$ (=KCTC $52884^T$ =NBRC $112871^T$).

• Structural Engineering and Mechanics
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• v.4 no.3
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• pp.227-242
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• 1996

An important class of problems in the field of geotechnical engineering may be analyzed with the aid of a simple integro-differential equation. Behavior of "rigid" piles(say concrete piles), "deformable" piles(say gravel piles), pile groups, pile-raft foundations, heavily reinforced earth, flow within circular silos and down drag on cylindrical structures (for example the crusher unit of a mineral processing complex) are the type of situations that can be handled by this type of equation. The equation under consideration has the form; $$\frac{{\partial}w(r,\;z)}{{\partial}z}+f(z){\int}^z_0g({\xi})(\frac{{\partial}^2w(r,\;{\xi})}{{\partial}r^2}+\frac{1}{r}\frac{{\partial}w(r,\;{\xi})}{{\partial}r})d{\xi}+h(r,\;z)=0$$ where w(r, z) is the vertical displacement of a soil particle expressed as a function of the polar cylindrical space coordinates (r, z) and the symbols f, g and h represent soil properties and the loading conditions. The merit of the analysis is its simplicity (both in concept and in application) and the ease with which it can be expressed in a computer code. In the present paper the analysis is applied to investigate the behavior of a single rigid pile to bedrock. The emphasis, however, is placed on developing the equation, the numerical techique used in its evaluation and validation of the technique, hereafter called the ID technique, against a formal program, CRISP, which uses the FEM.

• Atmosphere
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• v.25 no.3
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• pp.461-472
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• 2015

The sudden stratospheric warming (SSW), which is characterized by an abrupt increase of polar stratospheric temperature by several tens of degrees in a week, has been known to affect tropospheric weather and climate on sub-seasonal time scale in the boreal winter. Such downward coupling has been often examined in North Atlantic and Europe, but rarely examined in East Asia. In this study, by applying the two definitions of SSW to the reanalysis data, the possible impacts of the SSW events on the surface air temperature (SAT) and tropospheric circulation in East Asia are analyzed. It is found that Eurasian continent, including Siberia and the Northeast Asia, tends to experience anomalously cold SAT for up to sixty days after the SSW events. The resulting SAT anomalies largely resemble those associated with negative Artic Oscillation. However, over East Asia, SSW-related SAT change is weak and not statistically significant. Only during the extreme SSW events when the downward coupling between the stratosphere and troposphere is strong, East Asia exhibits significantly cold SAT anomalies. This relationship is presented by grouping SSW events into those followed by cold SAT anomalies over East Asia and those by warm anomalies for varying threshold values of the SSW events.

• Journal of Plant Biology
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• v.35 no.4
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• pp.307-316
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• 1992

A key to the species which have been isolated from the Korean forest soils was provided, and three unrecorded species found in the forest soils of Mt. Halla were described and illustrated. Twenty dictyostelid cellular slime molds had been found in Korean forest soils. Among these, nine species were PG positive, and eleven species were PG negative. Three species described in this study were all PG positive. These were Dictyastelium aurea-stipes Cavender, Raper et Norberg vaL aurea-stipes (new strain HL-5), Dictyastelium monachasiaides Hagiwara, PalysPhandylium tenuissimum Hagiwara. Morphological features and growth habits of these species were examined and compared, and their distribution patterns were illustrated.trated.

• Ocean and Polar Research
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• v.25 no.spc3
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• pp.409-416
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• 2003

Coastal development works generally increase the suspended sediment concentration of the ambient water, causing environmental impacts in various manners. The most active measures in three sides have been reviewed for their applicabilities in Korea. Referring to the cases in the USA, the legislation of environmental windows seems to be not proper for Environmentally Sound and Sustainable Developments (ESSD) until sufficient scientific data are obtained to address the individual issues of potential negative impacts. Feedback monitoring can be regarded as the best way for ESSD. Korea also has the basic legal system for the feedback monitoring as well as Environmental Impact Assessments (EIA). However, the frequency, period and parameter of related surveys should be improved for the true ESSD. Moreover, environmental facilities such as environmental dredgers should be widely used for mitigating environmental Impacts caused by coastal development works.