• Title/Summary/Keyword: needle-like

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Lysozyme Crystallization in Droplet-based Microfluidic Device (액적기반 미세유체장치에서 라이소자임 결정화)

  • Ko, Kwan-Young;Kim, In-Ho
    • Korean Chemical Engineering Research
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    • v.51 no.6
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    • pp.760-765
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    • 2013
  • Lysozyme crystallization was performed by using flow-focusing chip in droplet-based microfluidic system. Water-in-oil droplets were formed in the system and collected on petri-dish and cross type mold. Liquid-liquid reaction of lysozyme and sodium chloride occurred in the droplet and crystals were observed through microscope. Solution pH was varied as 4.8 and 7.2. Crystals of polyhedron and plate-like shape were obtained at pH 4.8, while needle structure crystals formed at pH 7.2. Lysozyme in single droplet for two pHs were crystallized with constant or decreased droplet size. However, crystals at pH 4.8 were only obtained in the droplet of which size was increased by the interaction between droplets. Droplet volume did not change at pH 7.2 and crystals formed in both droplets.

Physical Characteristics of Ridge Traversing Trail in Mount Jiri National Park (지리산 국립공원 종주 등산로의 물리적 특성)

  • Kim, Tae-Ho
    • Journal of the Korean Geographical Society
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    • v.46 no.4
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    • pp.425-441
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    • 2011
  • Ridge traversing trail in Mount Jiri National Park is classified as flat, gully-like, unilateral, and asymmetric bilateral, paths based on a location and gradient of paths. These types are interchangeable due to a drainage condition of trail surfaces. Using a rapid survey, the trail is 135.9 cm wide, 23.6 cm deep and $5.1^{\circ}$ in a gradient, respectively. All treads have been compacted due to human trampling. The path width is affected by a slope aspect and a distribution of Sasa borealis. An asymmetric path is wider than a symmetric path. A soil erosion rate is equivalent to $68.9cm^2/year$ for the period from 1960 to 2009, suggesting that the trail has been widened 2.7 cm/year and the tread lowered 0.4 cm/year. Trampling and needle ice action combined with rainwash induced by a pipeflow are dominant erosion processes contributing to the trail expansion.

Electrochemical Characteristics of Osteoblast Cultured Ti-Ta Alloy for Dental Implant (골아세포가 배양된 치과 임플란트용 Ti-Ta합금의 전기화학적 특성)

  • Kim, W.G.;Choe, H.C.;Ko, Y.M.
    • Journal of Surface Science and Engineering
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    • v.41 no.2
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    • pp.69-75
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    • 2008
  • Electrochemical behaviors of surface modified and MC3T3-E1 cell cultured Ti-30Ta alloys have been investigated using various electrochemical methods. The Ti alloys containing Ta were melted by using a vacuum furnace and then homogenized for 6 hrs at $1000^{\circ}C$. MC3T3-E1 cell culture was performed with MC3T3-E1 mouse osteoblasts for 2 days. The microstructures and corrosion resistance were measured using FE-SEM, XRD, EIS and potentiodynamic test in artificial saliva solution at $36.5{\pm}1^{\circ}C$. Ti-Ta alloy showed the martensite structure of ${\alpha}+{\beta}$ phase and micro-structure was changed from lamellar structure to needle-like structure as Ta content increased. Corrosion resistance increased as Ta content increased. Corrosion resistance of cell cultured Ti-Ta alloy increased predominantly in compared with non cell cultured Ti- Ta alloy due to inhibition of the dissolution of metal ion by covered cell. $R_p$ value of MC3T3-E1 cell cultured Ti-40 Ta alloy showed $1.60{\times}10^6{\Omega}cm^2$ which was higher than those of other Ti alloy. Polarization resistance of cell-cultured Ti-Ta alloy increased in compared with non-cell cultured Ti alloy.

Electrochemical Properties of Ti-30Ta-(3~15)Nb Alloys Coated by HA/Ti Compound Layer (HA/Ti 복합층 코팅한 Ti-30Ta-(3~15)Nb 합금의 전기화학적 특성)

  • Jeong, Yong-Hoon;Choe, Han-Cheol;Ko, Yeong-Mu
    • Journal of Surface Science and Engineering
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    • v.41 no.2
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    • pp.57-62
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    • 2008
  • Electrochemical properties of Ti-30Ta-$(3{\sim}15)$Nb alloys coated by HA/Ti compound layer have been studied by various electrochemical method. Ti-30Ta binary alloys contained 3, 7, 10, and 15 wt% Nb contents were manufactured by the vacuum furnace system. The specimens were homogenized for 24 hrs at $1000^{\circ}C$. The samples were cut and polished for corrosion test and coating. It was coated with HA/Ti compound layer by magnetron sputter. The HA/Ti non-coated and coated morphology of Ti alloy were analyzed by x-ray diffractometer(XRD) and filed emission scanning electron microscope(FE-SEM). The corrosion behaviors were investigated using potentiodynamic method in 0.9% NaCl solution at $36.5{\pm}1^{\circ}C$. The homoginazed Ti-30Ta-$(3{\sim}15wt%$)Nb alloys showed the ${\alpha}+{\beta}$ phase, and ${\beta}$ phase peak was predominantly appeared with increasing Nb content. The microstructure of Ti alloy was transformed from needle-like structure to equiaxed structure as Nb content increased. HA/Ti composite surface showed uniform coating layer with 750 nm thickness. The corrosion resistance of HA/Ti composite coated Ti-alloys were higher than those of the non-coated samples in 0.9% NaCl solution at $36.5{\pm}1^{\circ}C$. Especially, corrosion resistance of Ti-Ta-Nb system increased as Nb content increased.

Variation of Microstructure and Property of the Electro-slag Remelted M2 Steel with Heat Treatment Conditions (ESR한 M2강의 열처리에 따른 미세조직 및 물성 변화)

  • Lee, Ki-Jong;Kim, Moon-Hyun;Lee, Jeong-Keun;Joo, Dae-Heon;Kim, Myung-Ho
    • Journal of Korea Foundry Society
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    • v.22 no.6
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    • pp.281-287
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    • 2002
  • In order to investigate the variation of microstructure and property of the Electro-slag Remelted M2 steel, microstructure observation, hardness, and bending test were performed by using optical microscope. SEM/EDS, rockwell hardness tester, charpy impact tester and bending tester, respectively. It was revealed that the number of inclusions and content of gas elements(S, O, N) in M2 steel fabricated by ESR process decreased markedly compared to those of AIM. It seems to be due to refining effect of ESR process. The volume fraction of carbides in quenched and tempered specimens after austenitizing at 1150$^{\circ}C$ and 1240$^{\circ}C$ was measured. The volume fraction of grain boundary carbides were found to be similar for both specimens. However, The volume fraction of carbides in grain decreased with an increase of austenitizing temperature. When specimen was austenitized at 1150$^{\circ}C$, grain boundary carbides showed needle like morphology. But, the carbides were broken with an increase of austenitizing temperature. The specimen austenitized at 1240$^{\circ}C$ showed higher hardness and lower bending strength compared to that of 1150$^{\circ}C$. As expected, toughness increased with sub-zero quenching treatment.

Relationships between Dental Fear and Dental Services Utilization with Respect to Oral Health Promotion (구강건강증진 관점에서의 치과의료서비스와 치과공포의 연관성)

  • Choi, Jun-Seon;Kim, Kwang-Kee
    • Korean Journal of Health Education and Promotion
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    • v.23 no.4
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    • pp.47-65
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    • 2006
  • Objectives: This research is to provide the basic data for the improvement on oral health by examining the relationship between the dental fear and the dental service utilization, and to discuss a strategy for dental health in order to lower the level of dental fear. Methods: Data were collected by conducting a structured survey of 1,607 people between the age of 13 and 70 who have experiences in visiting a dental institution. Both univariate and multivariate analyses were employed to analyze the relationship between the number of times dental service and dental fears. Results: The average level of dental fear the respondents reported was $52.16{\pm}15.71$ and 31.5% of the response was at the level of high dental fear. A strong physiologic response during the dental treatment was muscle tension and the stimulations that strongly arouse the dental fear were the anaesthetic needle and the sound of drill. A result of analysis on the relationship between the dental fear and the dental service utilization shows that as the level of dental fear became higher, the number of times for the dental service utilization for the last year had reduced. Also when a patient has an experience of putting off or canceling an appointment due to dental fear and as a physiologic response during the dental treatment became stronger, the result shows a tendency that the number of times for the dental service utilization for the last year had reduced. Conclusions: It has been observed that dental fear is one of the main barrier to use dental service. Therefore, a physio-psychological factor like the dental fear should be included in the task of promotion of dental health, and in analyzing the dental service utilization behavior. A discussion about how to lower dental fear was made with respect to oral health promotion. At individual level, improvement of communication skill by dental service providers should be made with rearrangement of clinic environment into more cozy one.

Treatment of large sized cystic lesion of the jaws with specific appliance for decompression:cases report (악골 내 거대 낭종성 병소의 감압술을 위해 고안된 장치를 이용한 치험례: 증례보고)

  • Jang, Chang-Su;Kim, Ju-Won;Yang, Seung-Bin;Yim, Jin-Hyuk;Kim, Jwa-Young;Yang, Byoung-Eun
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
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    • v.37 no.2
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    • pp.133-136
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    • 2011
  • Cystic lesions on the jaws with expansion can invade the adjacent anatomical structure, infiltrate and expand the jaws, cause facial deformity, etc. There is great potential for pathologic fractures after cyst enucleation, and damage to the major structures like the nerve, artery. For these reasons, marsupialization and decompression are commonly used to reduce the cystic size. In 1947, Thomas first mentioned decompression that reduces the osmotic pressure in a cyst by making a hole in the cyst and insert a drain. In our cases, a large sized cystic lesion was treated with a specific device made from an orthodontic band and spinal needle. This device is easy and effective for applications and self irrigation.

Primary Lung Tumors in Five Dogs (개의 원발성 폐 종양 5증례)

  • Kim You-seok;Bhang Dong-ha;Kim Min-kyu;Seo Kyoung-won;Joo Min-suk;Park Jung-hyun;Choi Ul-soo;Jung Joo-hyun;Choi Eun-wha;Yoon Junghee;Youn Hwa-young;Choi Min-cheol;Lee Chang-woo
    • Journal of Veterinary Clinics
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    • v.22 no.3
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    • pp.288-295
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    • 2005
  • Five dogs at the age of 2-13 years, with variant respiratory signs like as dry cough, dyspnea, exercise intorelance and lameness were presented. On thoracic radiographs, all dogs had abnormalities such as soft tissue density, round mass image, increased pulmonary infiltration and pleural effusion. All dogs were diagnosed as adenocarcinoma on cytopathologic examinations by fine needle aspiration with ultrasound guidance. Computed tomography was performed in three cases to evaluate clinical stage. Primary papillary adenocarcinoma was confirmed after necropsy in one case.

Effects of Propofol on Electroencephalogram in Dogs (Propofol이 개의 뇌파에 미치는 영향)

  • 장환수;장광호;채형규;권은주;김정은
    • Journal of Veterinary Clinics
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    • v.17 no.2
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    • pp.359-367
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    • 2000
  • The aim of this study was to evaluate the effects of propofol on cortical electroencephalogram (EEG) in seven dogs. Propofol infusion was accomplished from low concentration to high concentration in series, and each concentration was infused for 20 minutes (M0: 0, M0.5: 0.5, M1.0:1.0, and M1.5: 1.5 mg/kg/min of infusion rate). EEG was recorded via needle electrode placed at Cz, which was applied to International 10-20 system. Arterial blood pressure. blood gas analysis and ECG were also measured. Hoemodynamics, Pa$CO_2$, PaO$_2$, heart rate and respiratory rate were variable, but were net significant(p>0.05). The power spectra of EEG in every concentration was compared wish those of control (MO). The powers at a1l frequencies at M1.0 and Ml.5 were decreased. Especially, the powers of the frequencies over 20 Hz were significantly decreased (p<0.O5). Powers at frequencies between 8 and 15Hz at MO.S were significantly increased (p<0.05) in response to the painful stimuli. It was inferred that they may reflect activity of the brain which is consciously processing the external Stimuli. Like the Power spectra, al1 the band powers of He EEG ($\delta$ 1-4, $\theta$4-8, $\alpha$ 8-13, $\beta$L13-21. $\beta$H 21-30, \ulcorner 30-50, and total 1-5OHz) were decreased in proportion to the increase of infusion rate at M1 .0 and M1.5. Especially, decrease of $\beta$H and ${\gamma}$ were significant(p<0.01). At M0.5, $\alpha$ band was significantly increased(p<0.05) among all the bands. Seizure activities which were concide with occurrence of spike wave were shown in all dogs at Ml .0 and M1.5.

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The effect of Cu and Sb on the microstructure and mechanical properties in Sn-Sb-Cu-Ni-Cd whitemetal (Sn-Sb-Cu-Ni-Cd whitemetal에서 Cu와 Sb가 미세조직과 기계적 특성에 미치는 영향)

  • Kim, Jin-Kon;Kang, Dae-Sung;Kwon, Young-Jun;Kim, Ki-Sung;Sang, Hie-Sun;Cho, Hyun
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.18 no.1
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    • pp.33-37
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    • 2008
  • The effects of Cu and Sb on the microstructure and mechanical properties of Sn-Sb-Cu-Ni-Cd whitemetal were investigated. Any compound phase was not observed in the whitemetal with 0.05 wt% Cu, while as the Cu content was increased, star- or needle-like $Cu_6Sn_5$ phases were found. The tensile strength gradually increased with Cu up to 5 % and then remained almost constant with Cu content above 5 %, while the hardness continuously increased with Cu content because of the increased hard $Cu_6Sn_5$ phases. As the Sb content increased, SbSn cuboids were present as well as $Cu_6Sn_5$. The tensile steength and hardness continuously increased and the elongation decreased with Sb content.