• Korean Journal of Ecology and Environment
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• v.52 no.3
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• pp.192-201
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• 2019

While a fairly large amount of organic matter is produced daily via phytoplankton photosynthesis in Lake Paldang, South Korea, knowledge of the role of algal-derived organic matter (OM) as a refractory OM source is not adequate. To understand the contribution of algal-derived OM to the refractory pool, biodegradation experiment and $KMnO_4$ oxidation experiment were conducted for 60 days using $^{13}C$ and $^{15}N$ labeled natural phytoplankton assemblage. The assemblage was collected from Lake Paldang on May 20, 2010. The photosynthetically produced total organic carbon ($TO^{13}C$), particulate organic carbon ($PO^{13}C$), and particulate nitrogen ($P^{15}N$) remained at 26%, 20%, and 17% of the initial concentrations, respectively, in the form of non-biodegradable organic matter. In addition, 12% and 38% of $PO^{13}C$ remained after $KMnO_4$ treatment on Day 0 and 60, respectively. These results indicate that photosynthetic products could be an important source of refractory organic matter after microbial degradation. Moreover, the microbially transformed algal-derived OM could contribute to the oxidation rate of the chemical oxygen demand.

• Korean Journal of Poultry Science
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• v.31 no.3
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• pp.137-143
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• 2004

The fractional synthesis rates(FSR) were measured with 2l-wk and 3l-wk-old broiler breeder pullets and hens to investigate the effect of sexual maturity on FSR. The FSR were obtained from chicken tissues and blood samples using High-Performance Liquid Chromatography/Mass Spectrometry(HPLC/MS). A L-l-13C, 15N -leucine saline solution was infused by bolus injection as a tracer into broiler breeder pullets in the experiment. A rapid HPLC/MS method was developed to measure the isotopic enrichments of leucine in plasma, tissue samples, and eggs. The enrichments of stable isotope leucine incorporated into protein and the enrichments of the stable isotope free leucine were measured in liver, breast muscle and blood samples. Two sets of experiments were conducted. In experiment one, 2l-wk-old, sexually immature broiler breeder pullets were divided into groups of three and blood samples were collected at 20 or 30 min intervals until 1.5 h from initial injection. The pullets were sacrificed in groups of three at varying time intervals for 7 h after injection. The liver, breast muscle and blood samples were removed for analysis. The FSR were estimated to be 8.7l%/day for liver, 4.06％/day for breast muscle, and 5.08％/day for blood samples in 30 minutes after injection from the enrichment ratios. In experiment two, sexually matured 3l-wk-old broiler breeder hens were assorted into groups of three and blood samples were obtained at 20 or 30 min intervals for 2 h. The FSR for blood samples were determined. The broiler breeder hens were sacrificed in groups of three at various time intervals until 7 h after injection and liver, breast muscle and blood samples were removed for analysis. The FSR were calculated to be 5.96％/day for liver. Eggs were collected from five chickens daily for 10 days after large bolus injection. The average of total enrichments of stable isotope in egg albumin was increased by 0.064% at 4 days after injection and was back to normal in 7 days.

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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• 2006.04a
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• pp.54-56
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• 2006

Single-well-gas-sparging tests were developed and evaluated for assessing the feasibility of in-situ aerobic cometabolism of trichloroethylene (TCE), using propane as a growth substrate. To evaluate transport characteristics of dissolved solutes [sulfur hexafluoride (SF6) or bromide (non-reactive tracers), propane (a growth substrate), ethylene, propylene (nontoxic surrogates to probe for CAH transformation activity), and DO], push-pull transport tests were performed. Mass balance showed about 90% of the injected bromide and about 80% of the injected SF6 were recovered, and the recoveries of other solutes were comparable with bromide and slightly higher than SF6. A series of Gas-Sparging Biostimulation tests were performed by sparging propane/oxygen/argon/SF6 gas mixtures, and temporal ground water samples were obtained from the injection well under natural gradient 'drift' conditions. The decreased time for propane depletion and the longer time to deplete SF6 as a conservative tracer indicate the progress of biostimulation. Gas-Sparging Activity tests were performed. .Propane utilization, DO consumption, and ethylene and propylene cometabolism were well demonstrated. The stimulated propane-utilizers cometabolized ethylene and propylene to produce ethylene oxide and propylene oxide, as cometabolic by-products, respectively. Gas-Sparging Acetylene Blocking tests were performed by sparging gas mixtures including acetylene to demonstrate the involvement of monooxygenase enzymes. Gas substrate degradation was essentially completely Inhibited in the presence of acetylene, and no production of the corresponding oxides was also observed. The Gas-Sparging tests supports the evidences that the successive stimulation of propane-oxidizing microorganisms, cometabolic transformation of ethylene and propylene by the enzyme responsible for methane and propane degradation.

• 한국해양학회지
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• v.4 no.1
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• pp.9-16
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• 1969

The sand drift movement at Young Il Bay area was tentatively investigated using Co-60 glass sand. Grain size and specific gravity of the glass sand which has originally contained natural cobalt, Co-59, was made similar to those of the real coastal sand, and was irradiated in a beam port of the reactor Triga Mark II to make the glass sand radioactive by the reaction of 59Co(n, r)60Co. The radioactive cobalt glass sand was deposited on the preselected point of sea bed, and, after a couple of weeks, the drift of sand was traced by means of specially designed water-proof G-M detector. Net counts at each point were plotted to figure out equicount lines and consequently such aspects as the direction of sand drift movement, the scope of distribution and the relative drift velocity etc. were indicated.

• Korean Journal of Pharmacognosy
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• v.11 no.3_4 s.43
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• pp.133-140
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• 1980

In order to develop the radio-immunoassay procedure for the ginsenoside $Rg_1$ we prepared the $Rg_1-BSA$ conjugate and $Rg_1-tyramine$ conjugate by condensing the $Rg_1-azide$, which was prepared by a series of six step chemical modification of the $Rg_1-side$ chain, with bovine serum albumin(BSA) or with tyramine. Rabbits were immunized by repeated injection of $Rg_1-BSA$ conjugate with Freund's Complete Adjuvant for 5 month long to obtain very potent $anti-Rg_1$ serum. The radio-labelled haptene was prepared by direct radio-iodination $(125_J)$ of $Rg_1-tyramine$ according to the chloramine-T method. The radio-immunoassay procedure was successfully furnished by using DCC method (dextran coated charcoal) and the anti-body titer of the anti-serum was found as being $1600{\sim}3200$ by using 15000cpm tracer per test. Calibration test using non-labelled $Rg_1$ showed linear competetive binding response in the $(8-300){\times}34pg$. range of non-labelled $Rg_1$. The cross reaction test using 19 ginsenoside analogues enabled us a full structure-activity analysis on the antigen-antibody reaction that the anti-body in the serum would recognize the full structure of ginsenoside $Rg_1$ except the side chain moiety.

• Textile Coloration and Finishing
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• v.8 no.1
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• pp.83-89
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• 1996

Carbon black has been used as a particulate soil to prepare artificial soiled fabrics for detergent study but it has two major defects. The one is the difficulty of quantitative analysis of carbon black for evaluate the detergency, the other is that there is no reliable correlation between the removal of carbon black and oily soil which is the major component of natural soil. In this study ferric oxinate was used as a particulate soil since it is in black color and can be soiled on fabric by suspension in water or by solution in chloroform and it is easily analysed quantitatively by extracting it from soiled fabric with chloroform to get correct value of soil removal. The characteristics of soil removal of ferric oxinate were compared with that of carbon black and Sudan black, an oil soluble dye, which had been proved that it's detergency correlated with that of oily soil The soil removal of ferric oxinate and Sudan black estimated from quantitative analysis and from K/S value were in good agreement whereas the result calculated by simple reflectance was consistently low. The soil removal of ferric oxinate was exceeded from that of carbon black without regard to surfactants, Triton and Las, but the effect of washing conditions such as temperature and washing time on soil removal of both soils with different suffactants showed no considerable difference. Though the soil removal of Sudan black was little effected by the conditions, the soil removal in Triton exceeded considerably that of in Las, which is the characteristic of oily soil. Thus the soil removal of Sudan black was in good agreement with ferric oxinate in Triton, a non-ionic surfactant, and with carbon black in Las, an artionic surfactant. We concluded that ferric oxinate is a more realistic model particulate soil for artificial soiled cotton fabric washed with non-ionic surfactant than carbon black.

• Proceedings of the Korea Water Resources Association Conference
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• 2012.05a
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• pp.194-194
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• 2012

본 연구에서는 자연하천에서 Rhodamine-WT를 이용하여 추적자 실험을 수행하고 이를 바탕으로 오염사고대응예측모델에 대한 검증을 실시하고자 하였다. 최근 국내에서는 다양한 형태와 규모의 수질오염사고가 매년 수십건 이상 발생하고 있으며, 따라서 수치모형 기반의 수질오염 사고 대응 예측시스템에 대한 높은 신뢰성이 요구되고 있다. 수질사고에 노출되어 있는 지표수를 각종 용수로서 안전하게 공급하기 위해서는 정확한 수질예측이 필수적이며, 이를 위해서 수십 년간 연구되어 온 수질모델을 오염사고 대응예측시스템에 적합하도록 정확성과 신뢰성을 갖추기 위한 연구가 진행되어야 한다. 수치 모형을 이용한 물질의 이송 및 확산 모의에서는 오염물질 도달시간과 확산 농도를 결정하는 것이 가장 중요한 요소이므로 이송 및 확산 모의에 대한 검증 기법 개발 및 적용이 필요하다. 본 연구에서는 낙동강수계 지류하천인 감천에서 추적자 실험을 4회 실시하여 측정한 수리량과 농도 실측치를 이용하여 분산계수를 종 횡분산계수 산정이 가능한 2차원 유관추적법을 적용하여 산정하였다. 각 단면에 유속은 ADV-3차원 유속계인 Flow-Tracker를 사용하여 도섭으로 측정하였으며 하천의 흐름 방향의 직각으로 측선을 설치하고 펌프를 이용하여 채수를 한 다음 Rhodamie WT의 농도를 측정하였으며 측선의 위치 보정은 GPS를 통하여 보정하였다. 측정 자료를 이용하여 2차원 유관추적법으로 분산계수를 산정한 결과 각각의 측선에 따라서 다소 차이가 발생하였으며, 일부 구간에서는 이론식으로 계산한 분산계수와 근사한 값이 나타났다. 이는 사주가 매우 발달하고 만곡이 많은 실험구간의 특성상 Elder와 Fischer 식으로 계산한 값과 차이가 발생할 가능성이 높은 구간이기 때문인 것으로 판단된다. 또한 하폭에 대한 수심비가 커질수록 분산계수도 증가하고 평균유속에 대한 전단유속에 비에 비례하는 것으로 나타났다.

• Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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• v.5 no.2
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• pp.113-122
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• 2007

In this study, uranium migration experiments have been performed using a natural groundwater and a granite core with natural fractures in a glove-box constructed to simulate an appropriate subsurface environment. Groundwater flow experiments using the non-sorbing anionic tracer Br were carried out to analyze the flow properties of groundwater through the fracture of the granite core. The result of the uranium migration experiment showed a breakthrough curve similar to that of the non-sorting Br. This result may imply that uranium migrates as anionic complexes through the rock fracture since uranium can form carbonate complexes at a given groundwater condition. The distribution coefficient $K_d$ of the uranium between the groundwater and the fracture filling material was obtained as low as 2.7 mL/g from a batch sorption experiment. This result agrees well with the result from the migration experiment, showing a faster elution of the uranium through the rock fracture. In order to analyze retardation properties of the uranium through the rock fracture, the retardation factor $R_d({\sim}16.2)$ was obtained by using the $K_d$ obtained from the batch sorption experiment and it was compared with the $R_d({\sim}14.3)$ obtained by using the result from the uranium migration experiment. The values obtained from the both experiments were very similar to each other. This reveals that the retardation of the uranium is mainly occurred by the fracture filling material when the uranium migrates through the fracture of a granite core.

• Journal of the Korean Society for Marine Environment & Energy
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• v.4 no.4
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• pp.3-11
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• 2001

To know the seawater infiltration into tidal flat sediment in coastal area is very important, because it is significantly correlated with the infiltration and transportation of pollutants in soil, the supply of dissolved oxygen, nutrients and organic matter to benthic organisms for survival of benthic organisms and the seawater purification. So, we set up purpose to clarify the infiltration behavior of seawater by wave action in tidal flat, to clear the effects of slope of tidal flat and breaking wave height on seawater infiltration and to quantify the infiltration volume of seawater. For purpose, the seawater infiltration was studied with visualization method by using coloring tracer and transparent glass beads replaced as natural sediment in model tidal flat. Specific conclusions derived from this study are as follows. The semi-circular type infiltration of seawater by wave action into saturated sediment was a new infiltration behavior that was not considered in previous studies. The infiltration rate of seawater was increased with increasing of breaking wave height and slope of tidal flat. However, the effects of the slope was bigger than that of breaking wave height on seawater infiltration into tidal flat sediments. It was possible to calculate the infiltration volume of seawater by wave action in natural tidal flat sediment and in fields. Therefore, we can point out that wave action play an important role in the supply of dissolved oxygen, nutrients and organic matter to benthic organisms, transportation or diffusion of pollutants and seawater purification. So, we hope to be studied the supply of food to benthic organism, pollutant transport and seawater purification on the base of these results.

• Economic and Environmental Geology
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• v.30 no.1
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• pp.25-33
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• 1997

The purpose of this study is to examine the feasibility of using stable isotopes as a hydrologic tracer, and to elucidate the groundwater circulation system and the source of S component dissolved in thermal water of the Chonju Jukrim thermal spring district based on the O, H and S isotopic variabilities of environmental materials including bedrock, rainwater, surface water, shallow subsurface water and thermal spring water. The ${\delta}^{18}O$ and ${\delta}D$ of subsurface waters and surface water show highly restricted range and plotted on the same meteoric water line as a ${\delta}D=8{\delta}^{18}O+19$ line, and derivate from the mean annual isotopic composition of the rain water but are analogous to those of rain waters precipitated during winter season, indicating that ground waters are originated from the meteoric water and are strongly affected by the seasonal variation of air mass. Thermal spring waters are more depleted in ${\delta}^{18}O$ and ${\delta}D$ than those of shallow ground water and surface water. It can be explained by the difference of recharge area. The hydrochemical properties of subsurface waters and surface water devide into two groups: $Ca(HCO_3)_2$ type including shallow subsurface water and surface water, and $Na(HCO_3)$ type of thermal spring waters. The ${\delta}^{34}S$ values of thermal spring water show very high positive and quitely distinct from those of shallow subsurface water and surface water that are similar to those of bed rocks, indicating that sulfate dissolved in thermal spring water has not only a terrigenic origin, but also originates partially from the foreign source containing very heavy ${\delta}^{34}S$ component such as an ancient sea water. However, the presence of $H_2S$ can not be ignore the affact of the isotopic fractionation to explaine the heavy ${\delta}^{34}S$ of thermal spring water. Overall, the Oxygen and Hydrogen stable isotopes can identify the source and the circulation system of the natural waters and the S-isotopes can provide a crucial clue on tracing the dissolved material transports in the circulation system of the natural water.