• Journal of Microbiology and Biotechnology
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• 제31권10호
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• pp.1438-1445
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• 2021

A bifunctional glycoside hydrolase GH78 from the ascomycete Xylaria polymorpha (XpoGH78) possesses catalytic versatility towards both glycosides and esters, which may be advantageous for the efficient degradation of the plant cell-wall complex that contains both diverse sugar residues and esterified structures. The contribution of XpoGH78 to the conversion of lignocellulosic materials without any chemical pretreatment to release the water-soluble aromatic fragments, carbohydrates, and methanol was studied. The disintegrating effect of enzymatic lignocellulose treatment can be significantly improved by using different kinds of hydrolases and phenoloxidases. The considerable changes in low (3 kDa), medium (30 kDa), and high (> 200 kDa) aromatic fragments were observed after the treatment with XpoGH78 alone or with this potent cocktail. Synergistic conversion of rape straw also resulted in a release of 17.3 mg of total carbohydrates (e.g., arabinose, galactose, glucose, mannose, xylose) per gram of substrate after incubating for 72 h. Moreover, the treatment of rape straw with XpoGH78 led to a marginal methanol release of approximately 17 ㎍/g and improved to 270 ㎍/g by cooperation with the above accessory enzymes. In the case of beech wood conversion, the combined catalysis by XpoGH78 and laccase caused an effect comparable with that of fungal strain X. polymorpha in woody cultures concerning the liberation of aromatic lignocellulose fragments.

• International Journal of Industrial Entomology and Biomaterials
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• 제8권1호
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• pp.61-66
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• 2004

Bacillus thuringiensis 656-3 which was isolated from a soil sample of mushroom house and showed high toxicity to mushroom flies, Lycoliella mali and Coboldia fuscipes, was surveyed for insecticidal effect in the oyster mushroom house.3. thutingiensis 656-3 was mass-cultured in the fermenter containing soybean cake(2%) and wheat bran (2%) as media source. Semi-for-mutation of B. thuringiensis 656-3 was performed with metamorphic starch only. When the formulation suspension containing $5{\times}{10^7}$ cfu was sprayed on the mushroom in mushroom house, the insecticidal effect of B. thuringiensis 656-3 to mushroom flies,1. mali and C. fuscipes, was maintained over 90% by the fifth day after starting spraying. The yield of oyster mushroom house with B. thuringiensis 656-3 was significantly increased compared to control. B. thuringiensis 656-3 represents a powerful biological insecticide for the control of mushroom flies.

• 한국문헌정보학회지
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• 제22권
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• pp.143-177
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• 1992

The main purpose of the school library is to present the various kinds of instructional media for an effective instruction including not only books, but also audiovisual materials and equipments. But unfortunately our school libraries have only books for the students and faculties at this time. But hereafter school library should house the various kinds of audiovisual media as well as printed media for the promotion of scholarstic achivemint. With a view to promoting the teaching method, and at the same time improving the scholarstic achivement of the students, instructional media were used in the class. To verify the efficiency of the instructional media, transparencies were used in the social science class of the 1st grade in the middle school and the natural science class of the 1st grade in the high school. In consequence, the academic achievement of the experimental class in which instructional media were used is superior to that of controlled class in which the textbook was only used. In < Social Science I >, the mean difference between the experimental class and the controlled class is 4.199 marks. And the difference, 0.032 is verified as a significant value in concordance to the t-test. In < Natural Science I >, the mean difference of the experimental class over the controlled class is 6.333. And the difference, 0.034 is verified as a significant value in concordance to the t-test. As shown in the above experimental research, using various kinds of the instructional media is more efficient than using textbook only. As a result of the questionaire on using the instructional media, most of the students desire to use of them continually because the use of the instructional media induce the interest, promote the comprehension, and entice the development of better memories.

• 한국암석학회:학술대회논문집
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• 한국암석학회 1999년도 춘계학술대회
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• pp.63-63
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• 1999

• 한국멀티미디어학회논문지
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• 제21권5호
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• pp.571-582
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• 2018

Recently, development of deep learning has shown better image classification result than human. According to recent research, a hidden layer of deep learning is deeper, and a preservation of extracted features shows good results. However, in the case of general images, the extracted features are clear and easy to sort. This study aims to classify plant leaf images. This plant leaf image has high similarity in each image. Since plant leaf images have high similarity not only between images of different species but also within the same species, classification accuracy is not increased by simply extending the hidden layer or connecting the layers. Therefore, in this paper, we tried to improve the hidden layer of the algorithm called DenseNet which shows the recent excellent classification results, and compare the results of several different modified layers. The proposed method makes it possible to classify plant leaf images collected in a natural environment more easily and accurately than conventional methods. This results in good classification of plant leaf image data including unnecessary noise obtained in a natural environment.

• 한국약용작물학회지
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• 제11권1호
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• pp.31-39
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• 2003

바위솔의 추출물로부터 항산화 활성 및 동물 항암세포를 이용한 생물학적 특성을 조사 해 본 결과는 다음과 같다. 생육지별 DPPH 프리라디칼 소거 및 xanthine/xanthine oxidase 억제 활성비교에서 재배지에서 수확한 시료의 활성이 $6.07(IC_{50}:{\mu}g/m{\ell})$로 가장 높았다 생육시기별 DPPH 프리라디칼 소거 및 xanthine/xanthine oxidase 억제의 활성은 9월 수확물이 가장 활성이 높았다. 실리카겔 컬럼크로마토그래피를 이용한 항산화물질 분리결과에서 S-4 분획물의 DPPH와 xanthine oxidase 억제활성이 $5.02(IC_{50}\;:\;{\mu}g/m{\ell})$와 $6.18(IC_{50}:{\mu}g/m{\ell})$을 나타냄으로써 높은 분리효율를 보였다. 항산화활성이 가장 높았던 LH-4 분획물의 주요화합물은 GC/MS에 의하여 지방산, 폴리페놀화합물 및 페놀유도체로 동정되었으며 alpha-androsta-7,14-diene과 1,2,3-benzenetriol이 주 물질이었다. POD 및 SOD 활성은 생육지간에 재배지, 산, 바닷가 순으로, 생육시기간에는 시기가 늦을수록 높게 나타났다 SOD 동위효소의 밴드수는 전 생육지, 전 생육시기에서 공히 2개의 밴드가 나타났으나, 그 세기는 재배지와 9월의 수확물이 가장 높은 활성을 나타내었다. 정제된 LH-4 분획물은 HL-60 세포의 종양증식을 억제시켰으며 종양유발유전자와 IPTG로 콜로니 형성을 유도한 암세포(2-12 cells)에서 분획물 400ppm 처리와 negative control 처리간의 colony형성은 유의차가 없었다.

• 미생물학회지
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• 제50권2호
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• pp.95-100
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• 2014

Hrq1은 곰팡이 유전체에서 생물정보분석에 의해 발견된 새로운 RecQ helicase이다. 이 단백질은 인간의 RECQL4와 가장 상동성이 높으며 최근의 유전학적 생화학적 연구를 통해서 유전체 안정성을 유지하는데 어떤 역할을 할 것으로 예상되었다. 본 연구에서는 RECQL4와 상호작용하는 것으로 알려진 인간 유전자들과 상동성이 있는 효모 유전자들이 Hrq1과 상호작용하는지를 yeast two-hybrid assay를 이용하여 조사하였다. 총 11개의 유전자를 조사한 결과, nucleotide excision repair (NER) 인자 중의 하나인 Rad14이 Hrq1과 상호작용하는 것을 발견하였다. 또한 정제한 단백질을 이용한 pull-down assay로 Hrq1과 Rad14 사이의 직접적인 상호작용을 확인하였다. Hrq1과 Rad14 사이의 yeast two-hybrid 상호작용은 4-nitroquinoline-1-oxide에 의한 DNA 손상으로 더욱 증가하였으며, 이러한 상호작용의 증가는 또 다른 NER 인자인 Rad4에 의존적이었다. 이러한 결과들은 Hrq1이 Rad14과의 상호작용을 통하여 NER 과정에 어떤 역할을 할 가능성을 제시하고 있다.

• 천문학회보
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• 제44권1호
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• pp.66.4-66.4
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• 2019

NGC 6822 is a dwarf irregular galaxy whose metal abundance is lower than of the Large Magellanic Cloud. Hubble V is the brightest HII complex where molecular clouds surround the core cluster of OB stars. Because of its proximity (d = 500 kpc), we can resolve the star-forming regions on parsec scales (1 arcsec = 2.4 pc). Using the high-resolution (R = 45,000) near-infrared spectrograph, IGRINS, we observed molecular hydrogen emission lines from photo-dissociation regions (PDRs) and $Br{\gamma}$ emission line from ionized regions. In this presentation, we compare our data PDR models in order to derive the density distribution of the molecular clouds on parsec scales and to estimate the total mass of the clouds.

• Journal of Species Research
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• 제5권1호
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• pp.157-165
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• 2016

The hyporheic zone is an ecologically important area for investigating habitat biodiversity. However, only few studies have been conducted on this aspect in Korea. This study aimed to investigate the distribution of arthropod communities in the hyporheic zone of Nakdonggang River between 2012 and 2013. The meiobenthic arthropod communities found in the hyporheic zone were identified using a stereomicroscope and classified into 9 taxanomic groups. The abundance of arthropod communities was higher in the hyporheic zones of streams having well-formed sandbanks and gravelly areas. The arthropod communities found along the Nakdonggang River differed depending on the conditions of levees and the regions of the river from where they were collected. The frequency of species of the order Harpacticoida was high in the Nakdonggang main stream and western downstream region. The abundance of species belonging to Cyclopidae was high in the upstream region, midstream region, and eastern downstream region of the river. The frequency of species of the order Bathynellacea was high in the riverside parks or cement levees, but that of species belonging to Cyclopidae was high in the natural levees and gabion levees. Our findings suggested that arthropod communities preferred natural levees.

• 한국시스템다이내믹스연구
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• 제8권2호
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• pp.235-252
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• 2007

This study focused on the analysis of the abilities and characteristics of the systems thinking for pre-service elementary teachers. Systems thinking is defined as the ability to see the world as a complex system in which one understands that everything is connected to everything else. For this study, 345 subjects carrying of the 2nd grade were selected and participated in making out the protocol developed by Sweeney and Sterman(2000) for the estimating ability of the systems thinking. As a result, the proportion of the correct answers based on the total points of view was 63%. However, the proportion of correct answer was considerably different according to the gender. The women group showed 53%, while the men group was 76%. Especially, there was a great difference in the rates of correct answer depending on their academic tracks of the humanities or natural science courses in the high school. The average rates of the correct answers for the natural science and the human science were 84 and 46%, respectively. Consequently, the ability of the systems thinking for pre-service elementary teachers is highly related with their gender and learning experience in the high school. The results of this study may contribute the practical use of the systems thinking in the area of the science education.