• Journal of Microbiology and Biotechnology
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• v.24 no.12
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• pp.1654-1663
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• 2014

To examine the effect of tumor suppressor protein p53 on the antitumor activity of 2-methoxyestradiol (2-MeO-$E_2$), 2-MeO-$E_2$-induced cell cycle changes and apoptotic events were compared between the human colon carcinoma cell lines HCT116 ($p53^{+/+}$) and HCT116 ($p53^{-/-}$). When both cell types were exposed to 2-MeO-$E_2$, a reduction in the cell viability and an enhancement in the proportions of $G_2/M$ cells and apoptotic sub-$G_1$ cells commonly occurred dose-dependently. These 2-MeO-$E_2$-induced cellular changes, except for $G_2/M$ arrest, appeared to be more apparent in the presence of p53. Immunofluorescence microscopic analysis using anti-${\alpha}$-tubulin and anti-lamin B2 antibodies revealed that after 2-MeO-$E_2$ treatment, impaired mitotic spindle network and prometaphase arrest occurred similarly in both cell types. Following 2-MeO-$E_2$ treatment, only HCT116 ($p53^{+/+}$) cells exhibited an enhancement in the levels of p53, p-p53 (Ser-15), $p21^{WAF1/CIP1}$, and Bax; however, the Bak level remained relatively constant in both cell types, and the Bcl-2 level decreased only in HCT116 ($p53^{+/+}$) cells. Additionally, mitochondrial apoptotic events, including the activation of Bak and Bax, loss of ${\Delta}{\psi}m$, activation of caspase-9 and -3, and cleavage of lamin A/C, were more dominantly induced in the presence of p53. The Bak-specific and Bax-specific siRNA approaches confirmed the necessity of both Bak and Bax activations for the 2-MeO-$E_2$-induced apoptosis in HCT116 cells. These results show that among 2-MeO-$E_2$-induced apoptotic events, including prometaphase arrest, up-regulation of Bax level, down-regulation of Bcl-2 level, activation of both Bak and Bax, and mitochondria-dependent caspase activation, the modulation of Bax and Bcl-2 levels is the target of the pro-apoptotic action of p53.

• Journal of Korean Association for Spatial Structures
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• v.16 no.3
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• pp.89-97
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• 2016

With the recent trend to construct high-rise and large buildings, the steel structure system is widely used, but there are not enough studies on the vibration characteristics of the iFLASH system on the buildings. Therefore, in this study, we performed a vibration measurement of the natural frequency and damping ratio in the stage of iFLASH panel, composit, frame completion and cladding completion. The result findings suggest that the damping ratio after the cladding completion has a greater effect on the reduction of the damping ratio, than the stage after the frame completion.

• Korean Journal of Agricultural and Forest Meteorology
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• v.11 no.4
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• pp.252-255
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• 2009

In this study we investigated the natural habitats of Allium grayi, Allium monanthum, Allium tuberosum' and Allium schoenoprasmum to develop a fundamental database for their breeding and cultivation. Both Allium grayi and Allium monanthum were growing wild mainly in the areas with the altitude of ${\leq}\;300\;m$. The natural habitats of Allium grayi and Allium monanthum were limited to the altitude of 1,000 and 800 m, respectively. Allium thunbergii was growing at a wide range of altitude from the lowlands (${\geq}\;100\;m$) to the high elevated areas (${\leq}\;1,000\;m$) whereas Allium maximowiczii was growing wild only at the high altitude of ${\geq}\;900\;m$. The number and the size of the natural habitat of Allium grayi were greater than those of Allium monanthum. The natural habitats of Allium grayi were in the Gyeonggi, Gangwon, Jeolla, and Gyeongsang Provinces whereas those of Allium monanthum were found mainly in the Chungcheong and Jeju Provinces. Allium grayi was growing wild in both inland and seaside districts whereas Allium monanthum was mainly in the inland areas.

• Korean Journal of Mathematics
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• v.17 no.3
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• pp.315-330
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• 2009

Using the fixed point method, we prove the generalized Hyers-Ulam stability of the following cubic-quadratic functional equation $$(0.1)\;\frac{1}{2}(f(2x+y)+f(2x-y)-f(-2x-y)-f(y- 2x))\\{\hspace{35}}=2f(x+y)+2f(x-y)+4f(x)-8f(-x)-2f(y)-2f(-y)$$ in fuzzy Banach spaces.

• Animal cells and systems
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• v.7 no.2
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• pp.133-137
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• 2003

Secreted proteins and membrane proteins play key roles in the formation, differentiation, and maintenance of multicellular organisms. In this study, we undertook to characterize these protein types in the central nervous system of the marine snail Aplysia kurodai using a yeast-based signal sequence trap method. One hundred and three cDNA clones were obtained by screening 300,000 clones from the signal sequence trap cDNA library. Of these, twelve were identical to previously identified Aplysia genes, 19 were related to known proteins in other organisms, and 54 clones were novel. These 54 new genes had high signal peptide scores or were found likely to contain a transmembrane domain sequence. Only 18 of the 103 clones proved to be false positive. The study demonstrates that the signal sequence trap method is an effective tool for Isolating Aplysia genes encoding secreted and membrane proteins.

• The Journal of Natural Sciences
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• v.12 no.1
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• pp.103-110
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• 2002

The aim of this study was to analysis the landscape equipments of 42 school gardens in Taejon metropolitan city. Outdoor educational landscape equipments were found out at 10 schools out of 42 schools. The materials of main entrance were consisted of steel and stone at 19 schools. The stands at front of the main ground were existed at 21 schools. The pergolas and benches were existed at 20 schools. The living material fences were found out at 21 schools. Plant nameplates were found out at 22 schools. But the contents of the nameplates were so poor. The wetland and roof garden of the school were not existed. School landscape equipments should be introduced by the distinction of elementary school, middle school, and high school as well as by the space of the school site.

• ALGAE
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• v.28 no.2
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• pp.173-183
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• 2013

Sodium hypochlorite (NaOCl) is widely used to disinfect seawater in power plant cooling systems in order to reduce biofouling, and in ballast water treatment systems to prevent transport of exotic marine species. While the toxicity of NaOCl is expected to increase by ongoing ocean acidification, and many experimental studies have shown how algal calcification, photosynthesis and growth respond to ocean acidification, no studies have investigated the relationship between NaOCl toxicity and increased $CO_2$. Therefore, we investigated whether the impacts of NaOCl on survival, chlorophyll a (Chl-a), and effective quantum yield in three marine phytoplankton belonging to different taxonomic classes are increased under high $CO_2$ levels. Our results show that all biological parameters of the three species decreased under increasing NaOCl concentration, but increasing $CO_2$ concentration alone (from 450 to 715 ${\mu}atm$) had no effect on any of these parameters in the organisms. However, due to the synergistic effects between NaOCl and $CO_2$, the survival and Chl-a content in two of the species, Thalassiosira eccentrica and Heterosigma akashiwo, were significantly reduced under high $CO_2$ when NaOCl was also elevated. The results show that combined exposure to high $CO_2$ and NaOCl results in increasing toxicity of NaOCl in some marine phytoplankton. Consequently, greater caution with use of NaOCl will be required, as its use is widespread in coastal waters.

• Korean Journal of Materials Research
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• v.20 no.5
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• pp.278-288
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• 2010

High Pressure High Temperature (HPHT) treatment can significantly change the color of diamonds. We studied the variation of the optical properties according to the nitrogen arrangement in natural brown diamonds of various types (type IaAB, type IaB, type IaA > B, type IaA < B, IaA = B) after HPHT treatment. The diamonds with different arrangements of nitrogen were annealed at temperatures in the range $1700-1800^{\circ}C$ under a stabilizing pressure of 5 GPa. HPHT treated samples were analyzed using UV-Vis-NIR, FT-IR, and PL spectroscopy. The absorption and luminescence spectra were measured to compare the variations of nitrogen arrangement in the natural brown diamonds before and after HPHT treatment. After HPHT treatment, the brown coloration in all types of diamonds was reduced and a decrease in the peaks related to the A-aggregate of nitrogen was more predominant than the B-aggregate. Furthermore, the peaks related to N3 (415.4 nm), H4 (496.4 nm), and platelet decreased and the peaks related to H3 (503.2 nm) and G-band increased after HPHT treatment. In conclusion, spectroscopic analysis of natural brown diamonds after HPHT treatment showed that a yellow color was produced by absorption in the H3 centers and a green color was generated by interaction between absorptions of the H3 and H2 centers.

• Fisheries and Aquatic Sciences
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• v.15 no.1
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• pp.15-19
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• 2012

A new cyclic peptide (six-membered amino acid), gamakamide-E (L-Leu-L-Met (SO)-L-Me-Phe-L-Leu-D-Lys-L-Phe), was isolated as a strongly bitter tasting compound from cultured oysters, Crassostrea gigas. The molecular formula of $C_{43}H_{61}N_7O_8S$ was deduced from high resolution fast atom bombardment mass spectrometry (HR FAB-MS) ($[M+H]^+$ m/z 836.4356 ${\Delta}$= -2.4 mmu). Its unique structure including a hydantoin structure was firstly elucidated by nuclear magnetic resonance (NMR) analysis. Stereochemistries of constituent amino acids were determined by chiral high performanced liquid chromatography analysis of natural and synthesized peptides.

• The Bulletin of The Korean Astronomical Society
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• v.42 no.1
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• pp.36.3-36.3
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• 2017

The Telescope Array (TA) experiment reported the arrival direction distribution of ultra-high-energy cosmic rays (UHECRs) with energies above $5.7{\times}10^{19}eV$ in the northern sky. A clustering of TA events, the so-called hotspot, was found; however, its nature has not yet been understood. To understand the origin of the TA hotspot, we examine the sky distributions of the TA UHECR arrival direction and filamentary structures of galaxies in the local universe. By statistical tests for anisotropy, we find a close correlation of the TA events with the filaments of galaxies connected to the Virgo cluster. We discuss our finding and its implications.