• 한국응용과학기술학회지
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• 제21권4호
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• pp.320-326
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• 2004

We have extracted the natural pine-needles to use ethanol in solvent, and could obtain the refined oil component from pine-needles extract. We have tested the antimicrobial effect from microbe experiment and analyzed with ICP/OES, GC/MS. we could obtain the next conclusion from the result of this experiment. In the first result of this experiment we could know that in case of increasing concentration of pine-needles, the number of microbe decreased more and more. Also we could know that the refined oil component of pine-needles appeared the sterilization effect of S-aureus and E-coli after 96hrs and 120hrs. So we could know that the refined oil component of appeared effect to microbe. In the second result of this experiment we could know that inorganic materials of Ca, Mg, V, Mn etcs from pine-needles detected to ICP/OES analysis and the aromatic compounds from refined oil component of pine-needles was certificated to GC/MS analysis.

• 한국염색가공학회지
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• 제24권3호
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• pp.196-203
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• 2012

The purpose of this study was to investigate usefulness of the pine needles extract on Atopic Dermatitis(AD)-like skin lesions. To investigate the effect of pine needles extract in vivo, atopic dermatitis (NC/Nga) mice using DNCB (2.4-Dinitrochlorbenzene) was used. NC/Nga mice were challenged with DNCB during 2 weeks to develope AD-like skin lesion. After that, pine needles extract was applied to AD-like skin lesion on the backs of the NC/Nga mice during 3 weeks. The efficacy of pine needles extract in the NC/Nga mice was evaluated by measurement of the skin lesion severity(NC mouse score), the serum IgE level, epidermal thickness changes, and mast cell number. Blood was collected from the retro-orbital area and the level of IgE in the blood was measured. The epidermal thickness and mast cell number were observed by microscopic method after H&E stain. The serum IgE levels were decreased after treatment with pine needles extract. The epidermal thickness and mast cell number were decreased after treatment with pine needles extract. To conclude, the topical application of pine needles extract suppressed the progression of AD-like skin lesion.

• 복식문화연구
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• 제18권2호
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• pp.371-381
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• 2010

The pine needles can be used for four seasons in normal living and it can be taken friendly everywhere as it is distributed over 50% in Korea. The pine needles consist of vitamins, protein, minerals, essential oil and enzyme related to antimicrobial activity. It has effect like high blood pressure, neuralgia and hanged over by terpene, glucokinin, rutin, apigenic acid and tannin. Also the extract of them can be used for dyeing of fabrics. However, the extract components and effects of them are not well known yet. Therefore, the purpose of this study was to investigate the volatile components of the pine needles extract and functionality. The pine needles extract was dyed into various fabrics(nylon, silk, wool and soybean) and mordanted with Al, Cu, Cr, Fe and Sn. The extracted aroma compounds were compared by gas chromatography-mass spectrometry. The major volatile compounds of pine needles verified by using SPME were alpha-pinene, beta-pinene, beta-phellandrene, caryophyllene, ethanon, benzen. A total of 15 compounds were identified by using the SPME fibers. In the UV-visible spectra, the maximum absorption of wavelength of the pine needles ethanol extract appeared at 460, 630nm for chlorophyll component and at 237, 281nm for tannin component with the pine needles distilled water extract. Most of sample showed high antibacterial effect in none mordant but wool fabric showed high antibacterial effect in mordants. The result of UV block test showed a superior ability of blocking ultraviolet ray infiltration in all sample.

• 한국염색가공학회지
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• 제25권4호
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• pp.327-336
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• 2013

The purpose of this study was to evaluate the color image of the natural-dyed silk fabrics. The dye was extracted from pine needle by boiling pine needle with ethanol at $78^{\circ}C$ for 3hours and distilled water at $100^{\circ}C$ for 2hours. The 100% silk fabric was dyed of extract in pH 5 at $90-100^{\circ}C$ for 1 hr. As mordants used were compounds of Al, Sn, Fe, and Cr, color image of pine-needle dyed silk fabrics was classified into 5 factors (pure, gentle, sophisticate, comfortable, pastorale) and the factor pure is most important one of those. Most cheerful image in pure factor was from the fabrics dyed with ethanol extract and then, none and Cr mordanting. Dignified image was from the fabrics dyed with ethanol extract and then, Cu or Fe mordanting. In production, products dyed with ethanol extracts was preferred to those dyed with distilled water extracts. Color image and preference of the silk fabrics dyed with pine needles extracted was affected by extraction solvents and mordants.

• 한국균학회지
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• 제39권2호
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• pp.111-116
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• 2011

본 연구는 미강과 솔잎, 강황을 첨가한 현미에 배양한 상황버섯균사체 추출물의 전자공여능, 아질산염소거활성, tyrosinase 저해활성, ACE 저해활성 및 혈전 용해 활성을 검토하고자 수행되었다. 상황버섯 배양물의 전자공여능은 에탄올 추출물 보다 물추출물에서 더 낮았다. 아질산염 소거활성은 물추출물 보다 에탄올 추출물로 부터의 상황 버섯 배양물에서 가장 높았다 특히, 솔잎 첨가물 처리시 아질산염 소거활성은 에탄올 추출물의 pH 1.2에서 약 70%였다. 상황버섯 배양물의 Tyrosinas 저해활성은 에탄올 추출물 보다 물추출물에서 가장 높았고, 저해율은 솔잎 첨가된 열수 추출물에서 가장 높았다. ACE 저해활성은 물보다 에탄올 추출물에서 매우 낮은 효과가 있었다. 혈전용해활성은 미강과 솔잎 그리고 강황에서 유사하게 높았다. 특히 미강 첨가시 그 활성이 plasmin 보다 약 5% 증가되었다. 그러므로, 유용 성분 분리 및 정제와 같은 추가적인 연구를 통하여 기능성 물질의 천연소재로써 식품 및 화장품 산업에 이용될 수 있을 것이다.

• 펄프종이기술
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• 제48권1호
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• pp.93-99
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• 2016

The extraction efficiency depending on the extracting methods and the conditions of extraction was investigated. The common steam extraction was compared to the distillation extraction method. The effects of the samples size and the extraction time on the extract yield were also investigated by using UV-Vis spectrophotometer. One of the functional components of pine needle extract as the natural phenol base components were detected by the UV-VIS at around 235 nm wavelength range. The absorbance intensity at around 235 nm wavelength of the pine needle extract was used as the indicator of the extraction efficiency in this experiment. The distillation extraction showed the higher extract yield than the steam extraction. The grinding treatment of pine needles also resulted in the better extract performance, but the severe grinding showed a little decrease in the extract yield especially in case of the distillation extraction method. More than half of the extract was collected at the first stage of the extraction, that was the first 15 minutes in the total 60 minutes extraction.

• 한국균학회지
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• 제39권2호
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• pp.105-110
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• 2011

본 연구는 현미에 미강, 솔잎 그리고 강황을 첨가한 장수버섯 배양물의 전자공여능, 아질산염 소거활성, tyrosinase 저해활성, ACE 저해활성 및 혈전용해 활성을 검토하고자 수행되었다. 전자공여능은 에탄올 보다 열수를 이용한 추출물에서 더 높았다. 아질산염 소거활성은 솔잎첨가 에탄올 추출물에서 가장 높았고 소거활성은 거의 50%였다. tyrosinase 저해활성은 열수이용 추출물에서 가장 높았고 미강, 솔잎 그리고 강황 첨가에 의한 저해율은 장수버섯 열수 추출물에서 각각 72.49%, 87.34% 및 82.75%를 보였다. ACE 저해효과는 모든 물과 에탄올 추출물에서 매우 낮은 효과가 있었다. 혈전용해 활성은 미강, 솔잎, 강황에서 유사하게 높았다. 특히 미강 그리고 강황이 첨가 되었을 때 활성이 plasmin보다 약 10% 증가 되었다. 따라서 동물 실험과 같은 추가 연구가 진행 후, 천연소재로서 식품 및 화장품 산업에 이용될 수 있을 것이다.

• Food Science and Biotechnology
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• 제15권4호
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• pp.582-588
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• 2006

The antioxidant and antimicrobial activity of the fermentation extract (PFE) and the 50 and 80% ethanol extracts (PE 50, PE 80) of Pinus densiflora pine needles were evaluated. Electron donating ability, superoxide dismutase (SOD) ability, and antimicrobial activity were observed in PFE; those abilities differed in PE 80 and PE 50, depending on the ethanol concentration used for the extraction. PFE had the highest electron donating ability with a value of 92.20%, while PE 80 and PE 50 had values of 74.66 and 53.47%, respectively. For SOD activity, PE 80 exhibited a slightly higher value of 31.11% compared to that of PFE and PE 50, which were 29.65 and 25.43%, respectively. PFE, PE 50, and PE 80 were all found to inhibit bacteria, and the effectiveness of this inhibition was strongly related to the type of extracts used. PFE showed good antimicrobial effects for all of the tested Gram-positive strains and for most of the tested Gram-negative strains. These results suggest that PFE has superior functionality compared to the ethanol extracts (PE 80, PE 50), in terms of antioxidant and antimicrobial activity. On the basis of these results, pine needle fermentation extracts can be used for industrial applications as a functional material.

• Molecules and Cells
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• 제20권2호
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• pp.235-240
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• 2005

Extracts of pine needles (Pinus densiflora Sieb. et Zucc.) have diverse physiological and pharmacological actions. In this study we show that pine needle extract alters pacemaker currents in interstitial cells of Cajal (ICC) by modulating ATP-sensitive $K^+$ channels and that this effect is mediated by prostaglandins. In whole cell patches at $30^{\circ}C$, ICC generated spontaneous pacemaker potentials in the current clamp mode (I = 0), and inward currents (pacemaker currents) in the voltage clamp mode at a holding potential of -70 mV. Pine needle extract hyperpolarized the membrane potential, and in voltage clamp mode decreased both the frequency and amplitude of the pacemaker currents, and increased the resting currents in the outward direction. It also inhibited the pacemaker currents in a dose-dependent manner. Because the effects of pine needle extract on pacemaker currents were the same as those of pinacidil (an ATP-sensitive $K^+$ channel opener) we tested the effect of glibenclamide (an ATP-sensitive $K^+$ channels blocker) on ICC exposed to pine needle extract. The effects of pine needle extract on pacemaker currents were blocked by glibenclamide. To see whether production of prostaglandins (PGs) is involved in the inhibitory effect of pine needle extract on pacemaker currents, we tested the effects of naproxen, a non-selective cyclooxygenase (COX-1 and COX-2) inhibitor, and AH6809, a prostaglandin EP1 and EP2 receptor antagonist. Naproxen and AH6809 blocked the inhibitory effects of pine needle extract on ICC. These results indicate that pine needle extract inhibits the pacemaker currents of ICC by activating ATP-sensitive $K^+$ channels via the production of PGs.

• 디지털융복합연구
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• 제17권7호
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• pp.395-400
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• 2019

4차 산업혁명시대인 현대사회는 뷰티산업에 대한 뜨거운 관심 속에 새로운 기술 개발을 통한 화학 소재의 화장품 출시가 줄을 이으면서 화장품산업이 호황을 누리고 있지만 화학 화장품에 대한 안구 건조증, 피부 트러블, 소화 장애, 호르몬 교란 등 수 많은 부작용들이 노출되고 있는 실정이다. 이와 같이 인체에 유해(有害)한 기존 화학성분 화장품의 부작용을 보완할 수 있는 솔잎의 천연화장품 소재로서의 범용적(汎用的) 활용가치를 과학적으로 검증하고 바람직한 천연화장품의 롤 모델을 제시하고자 솔잎에 대한 DPPH 라디칼 소거 활성실험, ABTS assay, flavonoid 함량을 연구하였다. 실험 결과 $70^{\circ}C$와 $100^{\circ}C$ 솔잎 열수(熱水) 추출물은 DPPH radical과 ABTS radical 소거능 모두 농도 의존적인 증가가 나타났으며 $70^{\circ}C$와 $100^{\circ}C$의 솔잎 열수 추출물에서 각각 $32.0{\pm}2.5mg\;QE/ml$와 $116.8{\pm}5.5mg\;QE/ml$의 총 flavonoid가 검출되었다. 따라서 인체에 이로운 항산화물이 다량 검출된 솔잎 열수 추출물은 인체에 무해(無害)한 천연화장품의 소재로서 활용 가능성이 매우 높다고 사료한다.