• Title/Summary/Keyword: natural oil

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Production of Volatile Oil Components by Cell Culture of Agastache rugosa O. Kuntze

  • Shin, Seung-Won;Kim, You-Sun;Kang, Chan-Ah
    • Natural Product Sciences
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    • v.7 no.4
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    • pp.120-123
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    • 2001
  • To develop systems for economic production of useful essential oil compounds, callus was induced from the seedlings of Agastache rugosa and cultured on MS medium. The volatile oil fraction was extracted from the callus and investigated by mean of GC-MS. The composition of the oil was compared with that of the mother plant. As a result, sixty five compounds including ferruginol were identified in the essential oil fraction. The main component of the oil from the leaves of Agastache rugosa was methyl chavichol (53.6%). Methyl jasmonate and jasmonic acid were added to the culturing cell suspension, separately and the composition of induced oil were compared. The oils from cultured cells treated with jasmonates showed considerably different patterns. Especially, the peak of estragole was found in callus oil after treatment with methyl jasmonate as though the amount was limited to 0.58%. In general, the TIC pattern of GC-MS of the callus oil became more similar to the oil from the leaves after elicitation.

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Changes of Chemical Composition during Seedling Development in Soybean

  • Seong, Rak-Chun;Choi, Kyu-Hoon;Harry C. Minor
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.43 no.2
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    • pp.95-100
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    • 1998
  • Seedling establishment of soybean [Glycine mar (L.) Mer-rill] is an important factor for soybean production in the field. The objective of this study was to determine the distribution of chemical composition in the emerging organs during seedling development in soybeans. Three soybean cultivars (Hill, Paldalkong, and Jangyeobkong) were planted at the Research Farm of College of Natural Resources, Korea University, on May 26, June 5, and June 14. Protein, oil, sugar, and starch contents were measured in each organ at each developing stage. Mean dry weight of three soybean cultivars decreased until VE stage and increased after this stage. Protein content of whole seedling did not change significantly during the seedling growth stage, but the amount in cotyledons markedly decreased with each growth stage increment. About 88% of the cotyledon protein was translocated to the other parts of the seedling at the V2 stage. Oil content of cotyledons sharply decreased until the V1 stage. Sugar content of the seedling was not detected at VE stage and starch content of seedlings increased slightly at VE and VC stages. For the changes of each metabolic component, the amount for whole plants decreased until the V1 stage and started to increase after this stage. The results of this study provide evidence for the breakdown of carbohydrates and oil at the initial stage of seedling growth.

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A Study on the Quantitative Measurement of Oil Carry-over in Natural Gas Vehicle fueling Station Using A Gravimetric Method (무게측정법을 이용한 천연가스 자동차 충전소 오일전이 정량 분석에 대한 연구)

  • Hwang, Sung-Soo;Oh, Jun-Seok;Kim, Ki-Dong;Oh, Young-Sam;Choi, Kyung-Sik;Kim, Hack-Eun
    • Journal of the Korean Institute of Gas
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    • v.19 no.1
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    • pp.12-17
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    • 2015
  • The core of the CNG fueling station is the compressor and most of CNG compressors in Korea require lubrication. Lubrication oil of CNG compressor that can be transferred into the pressure regulators and the engines of fueling system can cause a negative effect on NGV(Nature Gas Vehicle) performance during refueling due to oil Carry-over. In order to avoid the problem, it is necessary to enhance the quality of the compressed natural gas by measuring quantitatively the amount of the transferred oil. In this research, a sampling device and sampling tube were developed, which can be used with a gravimetric method of detection to measure CNG oil Carry-over. In addition, CNG samples were taken at 6 pre-selected CNG fueling stations and analysed for their trace oil Carry-over. The measured total oil Carry-over ranged from 2.569 to 6.509 ppm. This test measurements were compared with those of previous studies to verify the results.

Effects of the Essential Oil Components from Ligusticum chuanxiong on Proinflammatory Mediators of RAW264.7 Macrophage Cells

  • Lim, Hye-Rim;Shin, Seung-Won
    • Natural Product Sciences
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    • v.16 no.4
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    • pp.259-264
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    • 2010
  • The essential oil fraction was obtained from the underground parts o of Ligusticum chuanxiong (Umbelliferae) by steam distillation, and its main components, Z-ligustilide and butylidene phthalide, were isolated by column chromatography. Its essential oil fraction and the isolated main components were examined for effects on their anti-inflammatory properties in RAW 264.7 macrophage cells to develop a new natural anti-inflammatory drug. The results showed that the L. chuanxiong essential oil fraction and its main components, Z-ligustilide and butylidene phthalide, inhibited the production of nitric oxide significantly in lipopolysaccharide (LPS)-treated RAW 264.7 cells. LPS-induced interleukin-$1{\beta}$ (IL-$1{\beta}$), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-$\alpha$) production was also decreased in a dose-dependent manner. In addition, western blot analysis revealed that the L. chuanxiong essential oil fraction and also its main components, Z-ligustilide, and butylidene phthalide reduced the expression levels of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS).

Construction of Oil-Spill Warning System based on Remote Sensing/Numerical Model and Its Application to the Natural Resource Damage Assessment and Restoration System

  • Goto, Shintaro;Kim, Sang-Woo
    • Proceedings of the KSRS Conference
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    • 1999.11a
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    • pp.243-248
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    • 1999
  • From the lessons after the Nakhodka oil-spill in Jan. 1997, oil slick detection by using remote sensing data and assimilating the data to the simulation program is important for monitoring the oil-drift pattern. For this object, we are going to construct the oil-spill warning system for estimating the oil-drift pattern using remotesensing/numerical simulation Model. Additionally we plan to use this system for restorating oil-spill damage domestically, such as estimating the ecological damage and making the priority fur restorating the oil-spilled shoreline. This report is intended to summarize the role of geo-informatics in the oil spill accident by not only paying attention to the effect of information provision/information management via the map, but also reporting the interim result in part based on the details discussed in the processes of recovery support and environmental impact assessment during the Nakhodka's accident.

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Investigations on Eco Friendly Insulating Fluids from Rapeseed and Pongamia Pinnata Oils for Power Transformer Applications

  • Thanigaiselvan, R.;Raja, T. Sree Renga;Karthik, R.
    • Journal of Electrical Engineering and Technology
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    • v.10 no.6
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    • pp.2348-2355
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    • 2015
  • Transformer mineral oil which is normally hydrocarbon based is non- biodegradable and pollutes the environment in all aspects. Though vegetable oils are eco-friendly in nature and potentially could be used in transformers as a replacement for the mineral oil, there usage is restricted because of their oxidative instability. The present work focuses on using rapeseed oil and pongamia (pongamia pinnata) oil as effective alternatives for the traditional mineral oil in power transformer. The oxidative stability of the rapeseed oil and pongamia oil is increased by using combinations of the natural and synthetic anti-oxidants as additives. The parameters like breakdown voltage, viscosity, flash point, fire point are measured for the rapeseed oil and pongamia oil with and without the additives as per IEC and ASTM standards. The results shown encouraging changes in the parameter values and ensures the use of the oils as a potential alternative insulation in power transformers.

Simulation of Natural Gas Injected Dual-Fuel DI 2-Stroke Diesel Engine (천연가스를 파이럿오일과 이원공급하는 직접분사식 2행정 디이젤기관의 시뮬레이션)

  • Choi, In Su
    • Transactions of the Korean Society of Automotive Engineers
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    • v.3 no.3
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    • pp.9-18
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    • 1995
  • The substitution of conventional fuel oil by alternative fuels is of immense interest due to liquid oil shortage and requirements of emission control standard. Among the alternative fuels, natural gas may be the most rational fuel, because of its widespread resource and clean est burning. Meanwhile, engine simulation is of great importance in engine development. Hence a zero-dimensional combustion model was developed for dual-fuel system. Natural gas was injected directly into the cylinder and small amount of distillate was used to provide the ignition kernel for natural gas burning. The intake air and exhaust gas flow was modeled by filling and emptying method. Although the single zone approach has an inherent limitation, the model showed promise as a predictive tool for engine performance. Its simulation was also made to see how the engine performance was influenced by the fuel injection timings and amount of each fuel.

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A Study of Salmon Oil Type Analysis by FT-IR and Carbon Isotopes Ratio (FT-IR과 탄소동위원소 분석을 통한 연어유의 구분에 관한 연구)

  • Cho, Eun-Ah;Cha, Yun-Hwan;Lee, Young-Sang
    • The Korean Journal of Food And Nutrition
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    • v.25 no.4
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    • pp.968-973
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    • 2012
  • This study analyzes the structure types of salmon oil to evaluate the purity of salmon oil products based on the 38 different types of imported salmon oil products distributed in the Republic of Korea. The major types of omega-3 foods in the salmon oil are ethyl ester (EE) and triglyceride (TG). If the salmon oil contained potential contaminants and was processed in order to remove it, EE type omega-3 fatty acids are found in concentration. This provides a good guide in assessing if products were made with EE type ingredients or re-esterified contaminated materials. The results of the FT-IR analysis showed significant difference in the C=O, C-O band positions in TG and EE. There were 19 TG type products and 19 EE type products. The analysis of carbon isotope ratio was performed on the types of TG and EE. There were different properties in the 19 TG type products. In one product, the carbon isotope ratio was -25.15 and the other 18 products showed -22.15~-23.96. The carbon isotope ratio of all 19 EE type products showed -21.91~-23.74. The results of the TLC analysis showed similar results with FR-IR. The re-esterified TG form was not detected in the TG type products, confirming that the TG type products contained natural salmon oil. This study aimed to provide the basic material in classifying the types of natural salmon oil and re-esterified salmon oil, by analyzing the pattern and proportion of FT-IR spectrum, carbon isotope ratio, and TLC.

Effect of Carbon Source Consumption Rate on Lincomycin Production from Streptomyces lincolnensis

  • Choi, Du-Bok;Cho, Ki-An
    • Journal of Microbiology and Biotechnology
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    • v.14 no.3
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    • pp.532-539
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    • 2004
  • For efficient lincomycin production from Streptomyces lincolnensis L1245, various vegetable oils, natural nitrogen sources, and surfactants were investigated at the pilot-scale level in the flask. Olive oil as the sole carbon source was the most suitable one for producing lincomycin. When 20 g/lof olive oil was used, the lincomycin concentration and lipase activity reached 1.01 g/land 182 U/ml, respectively, after 5 days of culture. Among the various unsaturated fatty acids, when linolenic acid was used, the cell growth and lincomycin production were markedly decreased. On the other hand, when 0.2 g/l of oleic acid was added to the culture broth, the maximum lincomycin concentration was 1.0 g/l, which was about 1.7-fold higher than that obtained without the addition of oleic acid. Among the various natural nitrogen sources, pharmamedia or soybean meal was the most suitable nitrogen source. In particular, in the case of a mixture of 10 g/l of pharmamedia and soybean meal, 1.5 g/l of lincomycin concentration and 220 U/ml of lipase activity were obtained. When Span 180 was used as the surfactant, lincomycin production, lipase activity, and oil consumption increased. The correlation between the consumption rates of oil and lincomycin production in a culture using olive oil as the sole carbon source was also investigated. The lincomycin production depended on the consumption rate of olive oil. Using these results, fed-batch cultures for comparing the use of olive oil and starch as a conventional carbon source were carried out in a 5-1 fermentor. When olive oil was used as the sole carbon source, 34 g/l of olive oil was consumed after 7 days of culture. The maximum lincomycin concentration was 3.0 g/l, which was about 2.0-fold higher than that of starch medium after 7 days of culture. The product yield was 0.09 gig of consumed carbon source, which was about 3.0-fold higher than that of starch medium after 7 days of culture.