• Journal of Animal Science and Technology
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• v.53 no.5
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• pp.409-418
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• 2011

This study was conducted to investigate the effects of dietary probiotics and immunomodulator on growth performance, carcass characteristics, fecal $NH_3$ content and pathogenic bacteria counts in ileum and cecum and ileum of broiler chicken (Korean native chicken, HanHyup No. 3). A total of 120 (day-old) chicks were randomly divided into 5 treatments with 3 replicates and there were 8 birds per replicate. Dietary treatments consisted of five diets; the corn-soybean based control diet (C), the diet containing antibiotics (Avilamycin) 10 ppm (T1), the diet containing probiotics 1 [(Lactobacillus ($4.45{\times}10^6$) + yeast ($1.51{\times}10^6$) + Bacillus subtilis ($3.50{\times}10^5$)] at 0.5% level (T2), probiotics 2 [(Lactobacillus ($6.70{\times}10^7$) + yeast ($3.10{\times}10^6$)] at 0.5% level in diet (T3), and the diet containing probiotics 3 [T2 + ${\beta}$-glucan + organic acid] (T4) and raised for 9 weeks. There were no significant differences among treatments in weight gain, feed intake and feed conversion. Carcass ratios of broilers were higher in T3 and T4 than other treatments, however, the differences were non-significant. Internal organs and liver, heart weight were significantly increased in T4 (p<0.05) compared to other treatments. The fecal $NH_3$ gas content was decreased (p<0.05) in antibiotics fed group than others. However, probiotic fed groups were not different when compared with control. The number of Salmonella and E. coli in cecum were reduced in the group supplemented with probiotics and immunomodulator compared to the antibiotics (p<0.05). In this experiment, we showed that diets containing pro-biotics and immunomodulator were capable of an alternative to antibiotics.

• Preventive Nutrition and Food Science
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• v.9 no.1
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• pp.45-52
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• 2004

Effects of dietary calcium lactate (CaL-A) and Chungkukjang (Korean native fermented soybean) on bone mass, calcium status, body weight, serum glucose and cholesterol levels in young male rats were investigated. Chungkukjang was fermented by mixing 4 types of Bacillus sp., and then dried at 45$^{\circ}C$. Calcium lactate was prepared from the ash of black snail. The rats were fed a commercial rat diet for 1 week and then the experimental diets for 4 weeks. Animals were divided into four dietary groups: one calcium-deficient diet (Ca-De) and one of three with calcium supplemented diets (5 g calcium/kg diet) with either calcium phosphate (Ca-P), CaL-A, or CaL-A + Chuntkukjang (CaL-AC). Calcium supplemented diets contained 39 g Ca-P/kg diet and 28 g/kg of calcium lactate in the CaL-A and CaL-AC diets. Body weight gains during the 4 weeks in the Ca-P, CaL-A, CaL-AC and Ca-De groups were 130.45 g,112.50 g, 143.40 g and 10.20 g, respectively. Feed consumption of the groups from high to low was CaL-AC > Ca-P > CaL-A > Ca-De. The Ca-De group had low femur weights and low serum calcium concentrations, while they were comparatively high in CaL-AC, Ca-P and CaL-A groups. The Ca-De groups excreted less calcium in urine than did the other rats, probably due to increased absorption of the mineral in Ca-P, CaL-A and CaL-AC groups. Microscopic observations revealed that there were many regularly spaced holes in the femur of Ca-De group, while there were much smaller regularly spaced holes in Ca-P group. However, no holes in femur were observed in the CaL-A and CaL-AC groups. Bone surfaces were especially smooth and clean in the CaL-AC group. Serum concentrations of glucose and total cholesterol were remarkably lower in the CaL-AC group than in the other supplemented groups. These results suggest that calcium from CaL-A has higher bioavailability than from Ca-P, and dietary Chungkukjang may have a beneficial effect on calcium metabolism.

• Applied Biological Chemistry
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• v.8
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• pp.11-20
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• 1967

In order to study the change of nitrogeneous compounds during the "Natto" preparation, the contents of insoluble protein, water soluble protein and amino nitrogen were determined and the pattern of peptides and amino acids was investigated by paper chromatography for the fractions resulting from molecular sieving. The results are summarized as follows: 1. Insoluble protein nitrogen which was increased to 84% by autoclaving the native soybean decreased to 44%, whereas the trichloroacetic acid soluble nitrogen increased from 8% to 45% during Natto preparation, But the soluble protein nitrogen showed a slight increase. 2. Fractionation of the peptides using Dowex-50 resins showed that they consisted mostly of lower molecular weight peptides which increased in accordance with the progress of fermentation, especially after 30-hour period. 3. Sixteen known and two unknown amino acids, and three peptides with different Rf values were identified during the Natto preparation. Their appearance showed some difference in that phenylalanine appeared after 10 hours, methionine, after 20 hours and proline, after 30 hours, respectively. The three peptides appeared at the different stage of fermentation.

• Korean Journal of Environmental Biology
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• v.40 no.3
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• pp.239-246
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• 2022

Lactuca scariola L. is one of ecosystem-disturbance plants that grow everywhere such as roadsides, grasslands, railroads, banks, and fields. L. scariola usually occurs in autumn. It overwinters in rosette form. It flowers and produces seeds in early summer of the next year. Seeds of L. scariola can germinate immediately without dormancy when the temperature is over 20℃. Due to endogenous bacteria in seeds of L. scariola, it has a strong drought tolerance. Thus, it can grow well on roadsides. L. scariola should be controlled as it can result in 60-80% of soybean yield loss at densities above 50 plants m^-2. It is advisable to remove L. scariola as it competes with native plants by acting as a pioneer to other ecosystem-disturbance plants. Among various control methods, chemical control is the most effective method that is widely used. Soil treatment with herbicides such as oxyfluorfen EC and pendimethalin EC can inhibit the development of L. scariola. Foliar treatment herbicides glyphosate and glufosinateammonium are widely used. L. scariola is resistant to 2,4-D, dicamba, and MCPA among foliar treatment herbicides. Thus, it is recommended to apply herbicides with different modes of action.

• Journal of The Korean Society of Grassland and Forage Science
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• v.16 no.2
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• pp.93-104
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• 1996

A field experiment was conducted in Chungju and Jungwon to evaluate growth characteristics, dry matter yield, protein yield and palatability of intercropping comparing with monocropping forage crops by the use of sorghum $\times$ sudangrass hybrid(S.S.H.), five forage soybeans and two forage cowpeas of superior to selected experiment of varieties. All eight treatment plots were replicated three times and cutting date were cut July 6 and September 9. 1. The mean leaf number of S.S.H. in intercropping T2 treatment showed high compared to T1 treatment in all treatment except for Hwangkeum treatment, but T3 treatment was lower than the other treatments. In the leaf number of soybean, Jangbaek treatment was the highest as 47 leaves per plant, cowpea treatments showed high above 50 leaves. The stem diameter of S.S.H. in Jangyeob treatment of T2 treatment was 9.6mm. In legume, cowpea of T3 treatment was high as ranged from 7.9mm to 8.2mm. In stem hardiness of S.S.H., TI treatment was the lower than the other treatments, while Jangbaek treatment of T2 treatment was the highest as $2.0kg/cm^2$. In legume crops, Jangbaek and Baekun treatment were the highest as $1.6kg/cm^2$, but T3 treatment was very low as $0.3~0.6kg/cm^2$. 2. In the mean leaf ratio of S.S.H., T1 treatment was higher than T2 and T3 treatment as 34.9%. In the legume crops, Togyu treatment was the highest as 40.9%. In the mean tiller number of S.S.H., T2 treatment showed high compared to TI treatment, but T3 treatment of was rather decrease than The tiller number of S.S.H. in Jangyeob treatment of T2 treatment was the highest as 4.6 per plant. The dead stubble of S.S.H. was high in order to T3>T1>T2 treatment>, T3 treatment was highly about 2 times compared to T2 treatment. 3. S.S.H. as T3 treatment was resistant to lodging, but T3 and T1 treatment showed highly lodging. In the legums, T3 treatment was resistant to lodging, but T2 treatment showed highly lodging. In the S.S.H. of T2 treatment, the leaf of summer depression was not occurrence, but T3 treatment was higher than the other treatments. 4. The palatability of domestic animals was high in order of T2>Tl>T3, especially Togyu treatment of T2 treatment was the highest at the holstein and deer, and Jangyeob treatment of T2 treatment was the highest in the Korean native cattle. But T3 treatment was lower than other treatments in the holstein and Korean native cattle. 5. Fresh yield of monocropping(T1) was the highest as 94, 650kg/ha, while dry matter yield in Jangyeob treatment of T2 treatment was the highest as 15, 575/ha But fresh yield and dry matter yield of T3 treatment were the lowest(P<0.05). 6. Protein yield in T2 treatment was high, especially Jangyeob treatment of T2 treatment was the highest as 1, 605.5 kg/ha T3 treatment was low in spite of high protein content, because dry matter yield was lower than the other treatments. In conclusion, among T1, T2 and T3 treatment, Jangyeob, Togyu and Baekun treatments of T2 treatment showed optimum varieties for intercropping with S.S.H., because they showed high Dry matter yield, protein yield and palatability.

• Journal of the Korean Society of Food Science and Nutrition
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• v.38 no.10
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• pp.1381-1391
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• 2009

Dietary life style of the elderly living in rural areas of Gyeongbuk Andong during the busy farming season was surveyed and a monthly meal plan for the elderly was developed using eco-friendly native crops and considering the characteristics of a busy farming season and their preferences. A week meal plan was selected and applied to those fields. The meal plan was developed for an elder's house in Gyeongbuk Andong during the busy farming season. Especially, to increase the intake of calcium and dietary fiber, bamboo sprouts, pepper leaves, dried slices of whitebait, and fried anchovies were used. Considering the busy farming season, the ginseng chicken soup and the soybean noodle soup were served to provide high quality of protein and to improve health condition. A cold soup of cucumber and brown seaweed was used to supplement liquid, vitamins, and minerals. The results are as follows: the highest score of the preference on a staple was 8.77 for the boiled barley of menu 5; the favorite soup was the ginseng chicken soup (8.73) in menu 4; for side dishes, those in menu 5 were most popular (8.69); the favorite meal was the ginseng chicken soup of menu 4 (8.69). Regarding the amount of leftover foods, the lightest was 30.14 g in Gyeongbuk Andong menu 3 which significantly indicates high preference (p<0.05). The current study was a planned meal service menu using local crops and seasonal foods, which was actually applied to the field resulting in high preference and satisfaction levels.

• Korean Journal of Soil Science and Fertilizer
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• v.22 no.3
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• pp.239-244
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• 1989

This work was for the elucidation of heterogeneity in a natural population of Rhizobium japonicum existing in Seoul National University's Experiment Field and of sensitivities of R. japonicum isolates for several antibiotics by using a method based on intrinsic antibiotic resistance (IAR). In addition, the suitability of IAR method for the recognition of R. japonicum strain was elucidated. Twenty seven isolates from various soybean cultivars cultivated at SNU's Experiment Field were tested to 4 antibiotics (streptomycin sulphate, kanamycin sulphate, ampicillin, oxytetracycline);There were 21 different IAR patterns among 27 isolates. It demonstrated diverse distribution of R. japonicum strains in SNU's Experiment Field. Their growth was inhibited at from a low concentration of about $1{\mu}g/ml$ to a high concentration of $400{\mu}g/ml$ for streptomycin sulphate, ampicillin, and oxytetracyclin. For kanamycin sulphate, on the contrary, all 27 isolates showed their growth inhibitances at below the concentration of $12.5{\mu}g/ml$. Two isolates identified as different strains from each other by the previous seroimmunological tests showed the same sensitivities for 4 antibiotics, and it seemed that IAR method was not perfect for the exact recognition of R. japonicum strain.

• Korean Journal of Food Science and Technology
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• v.30 no.4
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• pp.908-915
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• 1998

By using Korean native soybean, traditional meju was prepared in Chuncheon, Kangweondo according to the traditional process. Analysis of physico-chemical, enzymatic and microbiological changes during meju fermentation were carried out in order to obtain a basic information for industrial scale production of meju. The enviroments for natural meju fermentation were $10{\sim}15^{\circ}C$ and $60{\sim}70%{\;}RH$. Moisture content decreased from 59% to 11% (exterior section) and 19% (interior section). the pH of meju rapidly increased up to 8.5 at $33^{rd}{\;}day$ of fermentation and thereafter decreased down to 7.9 at $70^{th}{\;}day$ of fermentation. Souble protein content was 1.47% at initial stage and increased up to $6.31{\sim}7.34%$ at $33^{rd}{\;}day$ of fermentation. Amino nitrogen content was $460{\sim}770{\;}mg%$ at $70^{th}{\;}day$ of fermentation. the color of meju became gradually black and decreased in redness and yellowness. During the process, protease and lipase seemed to play an important role in the digestion of soy protein and fat. Acidic protease activity increased up to $135.9{\sim}152.4{\;}unit/g$ at $33^{rd}{\;}day$ of fermentation and were $181.3{\sim}272.6{\;}unit/g$ at $70^{th}{\;}day$ of fermentation. Lipase activity increased up to 6 unit/g (interior section) and 15 unit/g (exterior section) at $70^{th}{\;}day$ of fermentation. the viable cell count of meju was at the level of $10^8{\;}CFU/g$ during the overall fermentation period. Aerobic halophilic count was $1.51{\times}10^7{\;}CFU/g$ at initial stage and maintained $10^8{\;}CFU/g$ level during the process. Initial anaerobic cell count was $2.0^9{\times}10^4{\;}CFU/g$ and increased up to $10^5{\;}CFU/g$ level at 47 days. Yeast and mold counts were $10^4{\sim}10^5{\;}CFU/g$ for the fermentation period.