• Parasites, Hosts and Diseases
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• v.37 no.1
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• pp.13-19
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• 1999

The effects of in vitro culture methods on morphological development and infectivity of Strongyloides venezuelensis filariform larvae ($L_3$) to rats were investigated. A significantly higher body length was observed in $L_3$ from filter paper culture ($597.3{\;}{\pm}{\;}32.2{\;}{\mu\textrm{m}}$) than those in fecal (($509.9{\;}{\pm}{\;}35.0{\;}{\mu\textrm{m}}$) and nutrient broth culture (503.3{\;}{\pm}{\;}31.0{\;}{\mu\textrm{m}}) (P<0.05). Larval infectivity was assessed by exposing rats to 1,000 $L_3$ from each culture and worms were recovered from the lungs and small intestines. Recovery rate of these worms did not show any significant difference. A significantly greater body length of adults was recorded in those corresponding to the $L_3$ harvested from filter paper (2,777.5{\;}{\pm}{\;}204.4{\;}{\mu\textrm{m}}$) and nutrient broth culture (($2.732.5{\;}{\pm}{\;}169.8{\;}{\mu\textrm{m}}$) than those corresponding to the $L_3$ obtained from fecal culture (($2.600.5{\;}{\pm}{\;}172.4{\;}{\mu\textrm{m}}$) (P<0.05). Although worm fecundity and EPG counts differed among culture methods but worm burdens and course of infection did not. These findings suggest that the methods of cultures have a significant effect on the morphological development of the larvae to the $L_3$ stage, but do not influence the infectivity to rats.

• KSBB Journal
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• v.25 no.5
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• pp.471-477
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• 2010

In order to improve bio-ethanol productivity by various cultivation methods in this paper, the culture modes using food wastes, such as batch culture, high-cell-density fermentation, SSF (simultaneous saccharification and fermentation) by fill & draw, continuous culture by fill & draw were performed and their productivities were compared. SSFs by fill & draw were performed by continuous decompression using 1 L evaporator system, and by 10 L bioreactor without decompression. In addition, the continuous cultures by fill & draw mode using SFW (saccharafied food wastes) medium were performed by changes of 40% culture broth with intervals of 12 h (0.03 $h^{-1}$), 6 h (0.07 $h^{-1}$), 3 h (0.13 $h^{-1}$). Consequently, productivities of bio-ethanol were 2.52 g/L-h and 1.30 g/L-h in batch culture and high- cell-density fermentation, respectively. The productivities of SSF by fill & draw showed 2.24 g/L-h and 2.03 g/L-h in continuous decompression with 1 L evaporator and 10 L bioreactor without decompression, respectively. Also, the productivities in continuous culture by fill & draw modes showed 2.02 g/L-h, 4.07 g/L-h and 6.25 g/L-h by medium change with intervals of 12 h, 6 h, and 3 h, respectively. In conclusion, the highest ethanol productivity was obtained in the continuous culture mode by fill & draw with dilution rate of 0.13 $h^{-1}$.

• Korea and Global Affairs
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• v.3 no.2
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• pp.131-154
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• 2019

The state behavior has a strong consequence with the national strategic culture. However, different scholars hold different views on the classification of the national strategic culture. As one of the most significant land neighbors in West China, Pakistan is China's all-weather strategic cooperative partner. Understanding Pakistan's strategic culture will not only help understand Pakistan's national policies and state behavior, but also help deepen China-Pakistan cooperation. Cutting in from the perspectives of geography, social economy, culture, history and military, the author believes that the formation mechanism of Pakistan's strategic culture is mainly affected by the following four factors: geopolitical environment, production mode and lifestyle, cultural tradition, historical experience and diplomatic relations. The analysis has found that Pakistan's strategic culture has been shaped by Islam and can be classified as an outward-oriented strategic culture, the state behavior also shows a strong Islamic identity.

• Microbiology and Biotechnology Letters
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• v.44 no.3
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• pp.246-253
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• 2016

The antibacterial activities of thirty mushroom species belonging to Basidiomycetes and Ascomycetes, cultivated on two liquid media, were evaluated against gram-positive (Bacillus subtilis and Staphylococcus aureus) and gram-negative (Escherichia coli) bacteria by the disk diffusion method. All of the mushrooms, except Auriporia aurea, Fomes fomentarius, and Lyophyllum shimeji, showed different antibacterial activity levels—from 9.5 mm in diameter of the inhibition zone to full inhibition of growth of the test bacteria. The antibacterial activities of Crinipellis schevczenkovi, Hohenbuehelia myxotricha, Oxyporus obducens, and Spongipellis litschaueri were observed for the first time. The antibacterial potential of culture liquids of the investigated species was higher than that of their mycelia activity. Dependence of the intensity of antibacterial activity on the culture medium was shown. The antibacterial efficiency of the most active species (Lentinus edodes, Piptoporus betulinus, and Phellinus igniarius) was verified and compared with those of some commercial antibiotics and natural essential oils of Salvia and Eucalyptus. The culture liquid of Piptoporus betulinus, obtained after cultivation on glucose-peptone-yeast culture medium, is a potential substance for further creation of antibacterial products.

• Journal of muscle and joint health
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• v.26 no.3
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• pp.195-204
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• 2019

Purpose: This study was conducted to identify the relationships between role conflict, nursing organizational culture, resilience and nursing performance and the factors influencing nursing performance in clinical nurses. Methods: The participants were 191 nurses working for more than 6 months in a National University Hospital. Data were collected through self-report questionnaires from October to November in 2016. Collected data were analyzed using descriptive statistics, t-test, ANOVA, Pearson correlation coefficients, and hierarchical multiple linear regression with SPSS/WIN 24.0. Results: Factors affecting nursing performance in clinical nurses were resilience (β=.41, p<.001), relation-oriented nursing organizational culture (β=.12, p=.007) and hierarchical-oriented nursing organizational culture (β=.13, p=.047). These factors explained about 37.7% of the variability in nursing performance (F=10.57, p<.001). Conclusion: It is necessary to estabilish a relation-oriented nursing organizational culture in nursing organization. Also, education and intervention programs to improve nurses' resilience should be developed in order to increase nursing performance in clinical nurses.

• New & Renewable Energy
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• v.17 no.3
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• pp.8-15
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• 2021

In this study, we assessed the effect of vitamin components (such as biotin, thiamine-HCl, and folic acid) on microorganism microbial growth and ethanol production was examined to enhance increase the ethanol concentration in the Clostridium autoethanogenum culture process using syngas as a sole carbon source. Biotin and folic acid concentrations of 0.2, 2, 20, and 100 ㎍/L were used in the culture experiments at 0.2, 2, 20, and 100 ㎍/L concentrations. The maximum ethanol concentrations of 2.81 g/L and 3.12 g/L were obtained by adding at 0.2 ㎍/L biotin and folic acid, respectively. Moreover, Thiaminethiamine--HCl at concentrations of 0.5, 5, 50, and 250 ㎍/L were was examined evaluated to in the culture experiments. The maximum ethanol concentration of 2.84 g/L was observed at 0.5 ㎍/L of thiamine--HCl. As a resultThus, the optimized concentrations of biotin, thiamine--HCl, and folic acid were determined at 0.2, 0.5, and 0.2 ㎍/L, respectively, for enhancing increasing the ethanol production. In conclusion, the maximum ethanol production was obtained by adding the minimal concentration of vitamins in C. autoethanogenum culture.

• Nuclear Engineering and Technology
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• v.54 no.7
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• pp.2467-2474
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• 2022

Nuclear Power Plants (NPPs) are the most protected facilities among all critical infrastructures (CIs). In addition to physical security, cyber security becomes a significant concern for NPPs since swift digitalization and overreliance on computer-based systems in the facility operations transformed NPPs into targets for cyber/physical attacks. Despite technical competencies, humans are still the central component of a resilient NPP to develop an effective nuclear security culture. Turkey is one of the newcomers in the nuclear energy industry, and Turkish Akkuyu NPP has a unique model owned by an international consortium. Since Turkey has limited experience in nuclear energy industry, specific multinational and multicultural characteristics of Turkish Akkuyu NPP also requires further research in terms of the Facility's prospective nuclear security. Yet, the link between "national cultures" and "nuclear security" is underestimated in nuclear security studies. By relying on Hofstede's national culture framework, our research aims to address this gap and explore possible implications of cross-national cultural differences on nuclear security. To cope with security challenges in the age of hybrid threats, we propose a security management model which addresses the need for cyber-physical security integration to cultivate a robust nuclear security culture in a multicultural working environment.

• Journal of English Language & Literature
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• v.55 no.3
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• pp.497-515
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• 2009

Culture as a source of identity, as Edward Said says, can be a battleground on which various political and ideological causes engage one another. It is not mere individual cultivation or private possession, but a program for social cohesion. Sensitively aware that a national culture should be independent from Europe, Walt Whitman enacts a new form of literature by placing different cultural values against Old World tradition. His interest in autochthonous culture originates from his deep concern about national consciousness. He believes that literary taste directed toward highly-ornamented elite culture is an obstacle to cultural unification of a nation. In order to represent American culture of the common people, Whitman incorporates a lot of cultural material into his poetry. Since he believes that America has many respectable writers at home, he urges people to adjust to their own taste instead of running after foreign authors. Whitman differentiated his poetry from previous literary models by disrupting the established literary norms and reconfiguring cultural values on the basis of American ways of life. In his comment on other poets, he concentrates on the originality and nativity of poetry. By claiming that words have characteristics of nativity, independence, and individuality, he envisions American literature to be distinguished from British literature in literary materials as well as in language. Whitman s language is composed of a vast number of words that can fully portray the nation. He works over language materials in two ways: reterritorialization and deterritorialization. Not only does his literary language become subversive of the established literary language, but also makes it possible to express strength and intensity in feeling.

• Journal of the Korean Society of Safety
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• v.30 no.3
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• pp.123-134
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• 2015

This study was conducted with the following three study objectives. First, effects of safety awareness level of lab researchers to the improvement of safety culture in the organization Second, effects of organizational safety behaviors to the improvement of safety culture Third, test of mediating effects of organizational safety behaviors in the relationship between safety awareness level and the improvement of safety culture. The results show that organizational safety behavior is an indispensable factor for the improvement level of safety culture. Especially, the factors in safety training activities, safety compliance and management system are mediating variables which affect the safety awareness level and improvement level of safety culture, which shows these variables are very important factors in reducing safety accidents through the improvement of safety culture. Therefore, safety behaviors in the organization should be considered with priority. If the organization leads to improve safety awareness through regular safety training and rewards and punishes according to the test results, safety awareness could be improved. This study was conducted to identify the necessary factors to improve the overall safety culture in the organization and contribute to the diffusion of safety culture by improving the safety training awareness of the researchers.

• International Journal of Oral Biology
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• v.45 no.4
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• pp.197-203
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• 2020

Mesenchymal stem cells in the dental pulp exhibit a tendency for differentiation into various dental lineages and hold great potential as a major conduit for regenerative treatment in dentistry. Although they can be readily isolated from teeth, the exact characteristics of these stem cells have not been fully understood so far. When compared to two-dimensional (2D) cultures, three-dimensional (3D) cultures have the advantage of enriching the stem cell population. Hence, 3D-organoid culture and 3D-sphere culture were applied to dental pulp cells in the current study. Although the establishment of the organoid culture proved unsuccessful, the 3D-sphere culture readily initiated the stable generation of cell aggregates, which continued to grow and could be passaged to the second round. Interestingly, a significant increase in SOX2 expression was detected in the 3D-spheroid culture compared to the 2D culture. These results indicate the enrichment of the stemness-high population in the 3D-sphere culture. Thus, 3D-sphere culture may act as a link between the conventional and 3D-organoid cultures and aid in understanding the characteristics of dental pulp stem cells.