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In Vitro Antioxidant and Antiproliferative Activities of Novel Orange Peel Extract and It's Fractions on Leukemia HL-60 Cells

  • Diab, Kawthar AE;Shafik, Reham Ezzat;Yasuda, Shin
    • Asian Pacific Journal of Cancer Prevention
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    • v.16 no.16
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    • pp.7053-7060
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    • 2015
  • In the present work, novel orange peel was extracted with 100%EtOH (ethanol) and fractionated into four fractions namely F1, F2, F3, F4 which were eluted from paper chromatographs using 100%EtOH, 80%EtOH, 50%EtOH and pure water respectively. The crude extract and its four fractions were evaluated for their total polyphenol content (TPC), total flavonoid content (TFC) and radical scavenging activity using DPPH (1,1-diphenyl-2-picrylhydrazyl) assay. Their cytotoxic activity using WST assay and DNA damage by agarose gel electrophoresis were also evaluated in a human leukemia HL-60 cell line. The findings revealed that F4 had the highest TPC followed by crude extract, F2, F3 and F1. However, the crude extract had the highest TFC followed by F4, F3, F2, and F1. Depending on the values of $EC_{50}$ and trolox equivalent antioxidant capacity, F4 possessed the strongest antioxidant activity while F1 and F2 displayed weak antioxidant activity. Further, incubation HL-60 cells with extract/fractions for 24h caused an inhibition of cell viability in a concentration-dependent manner. F3 and F4 exhibited a high antiproliferative activity with a narrow range of $IC_{50}$ values ($45.9-48.9{\mu}g/ml$). Crude extract exhibited the weakest antiproliferative activity with an $IC_{50}$ value of $314.89{\mu}g/ml$. Analysis of DNA fragmentation displayed DNA degradation in the form of a smear-type pattern upon agarose gel after incubation of HL-60 cells with F3 and F4 for 6 h. Overall, F3 and F4 appear to be good sources of phytochemicals with antioxidant and potential anticancer activities.

The Application of High-resolution Land Cover and Its Effects on Near-surface Meteorological Fields in Two Different Coastal Areas (연안지역 특성에 따른 상세 토지피복도 적용 효과 및 기상장에 미치는 영향 분석)

  • Jeong, Ju-Hee;Kim, Yoo-Keun
    • Journal of Korean Society for Atmospheric Environment
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    • v.25 no.5
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    • pp.432-449
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    • 2009
  • In this study, the effects of high-resolution land cover on the simulation of near-surface meteorological fields were evaluated in two different coastal regions using Weather Research and Forecasting (WRF) model. These analyses were performed using the middle classification land cover data upgraded by the Korean Ministry of Environment (KME). For the purpose of this study, two coastal areas were selected as follows: (1) the southwestern coastal (SWC) region characterized by complex shoreline and (2) the eastern coastal (EC) region described a high mountain and a simple coastline. The result showed that the application of high-resolution land cover were found to be notably distinguished between the SWC and EC regions. The land cover improvement has contributed to generate the realistic complex coastline and the distribution of small islands in the SWC region and the expansion of urban and built-up land along the sea front in the EC region, respectively. The model study indicated that the improvement of land cover caused a temperature change on wide areas of inland and nearby sea for the SWC region, and narrow areas along the coastal line for the EC region. These temperature variations in the two regions resulted in a decrease and an increase in land-breeze and sea-breeze intensity, respectively (especially the SWC region). Interestingly, the improvement of land cover can contribute large enough to change wind distributions over the sea in coastal areas.

A study on the Paradigm Shift in Design Management Through An Annotated Bibliography (문헌 주석을 통한 디자인 경영의 패러다임 변화에 관한 연구)

  • 정경원
    • Archives of design research
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    • v.13
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    • pp.93-103
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    • 1996
  • Why are there semantic difficulties in the jargon, design management\ulcorner A series of changes in the meaning of design management during 40 years. since the beginning of 1950s when the term was used officially in the U.K., has been studied through a set of annotated bibliography. As a result, it is identified that such semantic confusions have been situated in relation to the shift in the emphasis on design management as a narrow sense of design - a broad sense of design - strategic management. This may imply that design management becomes an intrinsic part of corporate management in line with the increasing importance of design in the success of business.

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A study on the Scythian costume (스키타이계(係) 복식(服飾)에 대(對)한 연구(硏究))

  • Kim, Moon-Ja
    • Journal of Fashion Business
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    • v.11 no.4
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    • pp.204-220
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    • 2007
  • The background of Korean Ethnical Costume was originated from those northern mounted nomadic groups, which was Scythe style Costume Culture. Through the antique records and paintings of tombs bequests hereby describe the forms of Scythian Cotume (1) Headgear : There was Conical Cap(or Pointed Cap), Feathered Cap, and Crown. (2) Clothes : Both Men and Women wore Jacket as upper garment with leftsided collars, narrow sleeves to the length of the hip line. As lower garment, they wore the tight Trousers and Kungo(:窮袴)that was attached with gusset. (3) Belts and Boots : On the upper garment bound the leather Belts that was hanged a hook that was shaped of animal form at the end. Scythian Buckles was divided into six groups, animal-shaped, animal's head shaped, animal fight-shaped, rectangle-shaped, rectangle openwork-shaped, genre scene shaped Buckle. To the Boots, they wore leather boots. (4) Ornaments : Ornaments divided into Dress Trimming(:Gold plaques), Earrings, Necklaces(;Torques), Bracelets, Rings. Scythian Gold Plaques were divided into several types according to the shape, animal style(curved beast shape, profile shape, head reversed over its back shape), round shape, quadrilateral form, star shape, flower shape, crescent shape, bundle shape, human appearance. Earrings consisted of a plain ring and pendant ring was a middle ornament hung from it to a pendants which hung was made of heart shaped leaves of the tree, beads-linked. Scythian Torques were divided into several types according to the shape, Torque with Terminal style, Spiral style, Layers style, Crescent-shaped pectoral style, Crown style. Scythian Bracelet were divided into 4 styles according to the shape, Bracelets with ends shaped like beasts style, Spiral style, Layers style, Crown with openwork style. Rings were rhomb-shaped and animal shaped styleRings (5) Animal motifs used in Scythian ornaments appears that in some cases the work was intended to be purely ornamental, while many times the motifs had symbolic meaning (such as the successful dominance of the aggressor over the victim portrayed in the attack scenes). Magical use of symbols may have been inten-ded to guarantee the power of the aggressor.

A Design of CPW Band-Pass Filter with Rejection Band for Ultra-Wideband System (저지 대역을 갖는 UWB용 CPW 대역 통과 여파기의 설계)

  • No, Jin-Won;Hwang, Hee-Yong
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.18 no.7
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    • pp.704-709
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    • 2007
  • In this paper, a CPW band-pass filter with a rejection band is proposed for UWB(Ultra-Wideband) communication systems. The proposed filter has a band-pass characteristic of wide-band by inserting only a slot in $50{\Omega}$ transmission line. To obtain the band-rejection function at WLAN frequency band($5.15{\sim}5.725GHz$), the designed filter is combined with folded slot resonators on the ground plane of the CPW structure. The fabricated CPW band-pass filter shows a compact size of $15.35{\times}13.60mm$, a wide passband of 2.8 GHz to 9.8 GHz and the narrow stop-band of 5.15 GHz to 5.71 GHz for 3-dB bandwidth. Also, the measured group delay is less than 400 psec throughout the operation frequency band except the rejection band.

Study on the Electromagnetic Wave Propagation In the Parallel-Plate Waveguide with the Metamaterial ENZ Tunnel Embedded (Metamaterial ENZ 터널이 포함된 평행 평판 도파관 내 전자기파의 전파 특성에 관한 연구)

  • Kahng, Sung-Tek
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.20 no.2
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    • pp.135-140
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    • 2009
  • This paper discusses how to change the electromagnetic waves' property in the cut-off causing discontinuity existing in the guiding structure of the RF passive component by using the metamaterial and elaborates on its principle. Particularly, we find and explain, from the viewpoint of electromagnetics and circuit theories, the so-called tunneling condition that when the segment with an extremely narrow cross-section leading to blockage in the parallel-plate waveguide is given the ENZ(Epsilon Near Zero) for its filling material, the wave starts to propagate through the segment. The analysis method as a transmission-line theory taking the discontinuity and material change into consideration is shown valid through the comparison with other methods for analyzing parallel-plate waveguides, and provides the illustration of the S-parameters and impedance describing the characteristics of the tunneling.

다채널 표면 플라즈몬 공명 영상장치를 이용한 자기조립 단분자막의 표면 분석

  • Pyo, Hyeon-Bong;Sin, Yong-Beom;Yun, Hyeon-Cheol
    • 한국생물공학회:학술대회논문집
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    • 2003.04a
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    • pp.74-78
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    • 2003
  • Multi-channel images of 11-MUA and 11-MUOH self-assembled monolayers were obtained by using two-dimensional surface plasmon resonance (SPR) absorption. Patterning process was simplified by exploiting direct photo-oxidation of thiol bonding (photolysis) instead of conventional photolithography. Sharper images were resolved by using a white light source in combination with a narrow bandpass filter in the visible region, minimizing the diffraction patterns on the images. The line profile calibration of the image contrast caused by different resonance conditions at each points on the sensor surface (at a fixed incident angle) enables us to discriminate the monolayer thickness in sub-nanometer scale. Furthermore, there is no signal degradation such as photo bleaching or quenching which are common in the detection methods based on the fluorescence.

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Sizing System for Medical Compression Stockings -Focus on Imported Medical Compression Stockings in the Korean Market- (의료용 압박스타킹의 사이즈 체계에 관한 연구 -한국 시장에서의 수입 의료용 압박스타킹을 중심으로-)

  • Do, Wol-Hee;Kim, Nam-Soon
    • Journal of the Korean Society of Clothing and Textiles
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    • v.36 no.8
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    • pp.860-874
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    • 2012
  • This study provides size information on current imported medical compression stockings in Korea. A total 20 brands from 6 countries imported and sold in the Korean on-off line markets were selected for this study and the sizing system of medical compression stockings was researched. The results of this study were as follows: there were size differences among different USA brands according to product type or compression class of medical compression stockings. To establish the sizing system for medical stockings of the German brands, the basic body measurements standard was set as cA, cY, cB, cB, cC, cD, cE, cF, cG, cH, cT, lD, lG, and lT. Italian brands developed the sizing system for medical compression stockings by compression class. In the Swiss brands, size types were sub-divided as 'Normal', 'Plus', 'Short', and 'Long'. The sizing 1 were confined within very narrow limits. There were differences of the basic body measurements between Taiwan brands. The results of the comparison of the size range of the imported brands with Size Korea (KATS, 2010) by two-way size distribution shows that the sizing systems of the imported brands were unsuitable for Koreans. To enhance the suitability of the sizing system for medical compression stockings, a new sizing system for Korean adult males and females needs to be established.

A Study on the Dress of the Period of the Three States From the Wall Painting of Afrasiab's Palace in Samarkand (사마르칸트 아프라시압궁전 벽화에 나타난 삼국시대복식연구)

  • Lee, Sang-Eun;Kim, Ae-Ryeon
    • Journal of the Korea Fashion and Costume Design Association
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    • v.8 no.2
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    • pp.183-199
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    • 2006
  • This study aims to estimate the nationality of two envoys by examining their dresses and appearances depicted on the wall painting of Afrasiab's Palace in Samarkand with the concentration on the history of the international exchange between three states and the countries in Central Asia. The international exchange between three states and the countries in Central Asia are examined with the concentration on the literature review. And what are related to the dresses during the period of the three states are examined with the concentration on the literature review and the wall painting of Goguryeo tomb. The followings results were identified. The dresses of envoys were comprised of Jowugwan, the soft hat shaped like a peaked hat, the upper garment reaching a hip line with Jikryeong Gyoim and Tongsu, narrow trousers, black belt with a hook, front-pointed shoes and big sword with a round ring. The items of dressing are very similar to those during the Goguryeo period as observed on the wall paintings and excavated articles from the tombs from the Goguryeo period and literature. The next examination is estimated that Goguryeo had the official relationship with the countries in Central Asia before the invasion of Tang in mid-7 century when the wall painting, in our estimation, was produced, and to this end, Goguryeo sent the envoys to those countries. The envoys on the wall painting were estimated to be from Goguryeo as discussed above.

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A Study on the Costume of Loulan - Centered Around Interrelationship with Ancient Korean Costume - (누란(樓蘭) 복식 연구 - 고대 한국복식과의 상관관계를 중심으로 -)

  • Chae, Keum-Seok;Kim, Eun-Kyoung
    • Journal of the Korea Fashion and Costume Design Association
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    • v.18 no.3
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    • pp.197-211
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    • 2016
  • Loulan was an ancient kingdom located on the northeast of the Taklamakan Desert, a diverging point between the north and south of Silk Road, on the east of the current Turpan, from the 2nd century BC and the 7th century A.D, absorbing and propagating culture of both sides through active trade. Meanwhile, as many previous research investigations suggest the early trade between Korea and the countries bordering on Western China, characteristics of Loulan costume have been found to have similarity to those of ancient Korean costume. Also, it can be compared to costume in other surrounding regions. Therefore, it is a highly valuable region in researching ancient costume. This study examined the historical background of Loulan and its relationship with ancient Korea, analyzed the clothes found in historic sites of Louland and Niya, and compared costume elements of Loulan and those of Korea. The result of the study shows that first, the Loulan Jeogori was based on the Jikryeonggyoim, with Chaksu, narrow sleeves and the length coming between below the chest and above the bottom, and the line decoration and waist belt were very similar to Korean costumes, as well as the Banryeong, curved collar and Bansu, short sleeves. Second, the types of pants included Gunggo, characterized by closed bottom and many creases, and Daegugo, wide-leg trousers, which are very similar in shape to the Korean Shilla lay sculpture, Stele To Martyr Yi Ch'a-don, Yangjikgongdo and Wangheedo. Third, in accessories as well, the Jowu stuck on the Gwanmo is similar in the shapes to Korean costume.

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