• The Korean Journal of Malacology
• /
• v.20 no.1
• /
• pp.45-53
• /
• 2004

This study have conducted to calibrate the productivities on the mussel (Mytilus galloprovincialis) cultures in Jinhae Bay. Annual water temperature, salinity and dissolved oxygen have varied to 7.2-$25.9^{\circ}C$, 32.9-34.0 psu, 7.9-8.7 mg/l, respectively. In April, the smallest size of spat, 3.3 mm in shell length, have recruited, and 82.5 mm have recorded to the maximum. In summer, variations of growing up between individuals showed both shell length and weights at same cohort. Annual production at 2 m, 5 m, 8 m depth calculated to 10.91, 10.85 and 9.19 gWWt/ind./yr, respectively. Mussel cultivated in the upper part of the water column (two meter depth) were significantly longer and heavier than those in lower part (eight meter depth). After the recruitment, monthly production showed gradual increment at all positions during summer. As results, annual production of mussel cultivated seemed to be controlled by conditions of growing up in summer. Based on the productivities calculated to this study, the total annual production of the mussel cultivated in Jinhae Bay have estimated about 196 ton/ha/yr.

• Journal of the Korean Society of Food Culture
• /
• v.30 no.2
• /
• pp.158-169
• /
• 2015

Tean Mado Shipwreck No. 3 is presumed to have been shipwrecked between 1260 and 1268. It departed from a Southern costal area of Yeosu in Jeonnam Province to Ganghwa Island, its final destination at which the temporal regime of Koryo Dynasty was located. In the shipwreck, a total of 35 wooden tablets were found, and forwarding places, senders, receivers, descriptions, and quantities of freight were written on the wooden tablets. The names of receivers included Kim Jun, who was influential in the late Musin Era of the Koryo Dynasty, and key institutions such as Junmin and Sambyulcho of the Musin force. Twenty wooden tables had lists of food items such as barley, abalone, salted-fermented abalone, mussel, dried mussel, salted fermented mussel, dried shark meat, fish oil, pheasant, and dried dog meat. The food items in the late 13th century were systematically examined using scientifically determined food organic remains and records of wooden tablets among the marine relics of Mado Shipwreck No. 3.

• Journal of the Korean Society for Marine Environment & Energy
• /
• v.5 no.1
• /
• pp.68-75
• /
• 2002

This study were observed to the conditions of organic pollution applying to tile environmental assessments based on macrobenthos around the raft culture ground which became an issue by organic matter input in Jinhae Bay. Three stations, mussel farm, oyster farm and the control station on which rarely culture activities were fixed and have analyzed to the sediment characteristics and macrobenthic communities. Muddy substrates between 6.0 and 6.60 on mean grain size have prevailed to the bottom layer in all stations. The highest organic matter content of 2.8% was observed to the mussel farm while the lowest one of 1.5% was recorded on the control area. As a total, 79 species from seven phyla were identified. Comparing to shell farms, the control station have composed to more diversified community structure. In the mussel and the oyster farm, opportunistic species, living in high organic contents area, Lumbrineris longifolia, Heteromastus. filiformis in polychaetes and Corophium sinense in amphipod were dominated. As results on environmental assessment, in spite of changing to the species composition and densities seasonally, the mussel farm have recorded only to moderately polluted condition.

• Korean Journal of Environmental Biology
• /
• v.35 no.2
• /
• pp.152-159
• /
• 2017

For the effective seedling production of the hard shelled mussel, Mytilus coruscus, this study assessed the effects of the dietary value of live food, density, water temperature and salinity on growth and survival rate of the larvae. The optimal survival rate and growth rate were examined under differing conditions of water temperature, salinity, and rearing density for 30 days. The three groups were provided different feeding organisms, such as Isochrysis galbana and Teleaulax suecica. The mixtures were provided at a rate of $5{\times}10^4cell\;mL^{-1}$. The best growth was observed in the group with conditions $21^{\circ}C$ water temperature ($16.2{\pm}9.1{\mu}m$), 33 psu of salinity ($16.82{\pm}3.9{\mu}m$), $2500individual\;m^{-2}$ ($17.2{\pm}5.9{\mu}m$), and fed with $5{\times}10^4cell\;mL^{-1}$ of I. galbana and T. suecica mixture ($16.0{\pm}7.3{\mu}m$). The highest survival rate was found in the group at conditions $18^{\circ}C$ water temperature (66.4%), 33 psu of salinity (24.4%), $2500individual\;m^{-2}$ (65.8%), and fed with $5{\times}10^4cell\;mL^{-1}$ of I. galbana and T. suecica mixture (58.8%). We therefore conclude that the suitable culture conditions for the stable production of hard shelled mussel artificial seedlings was at 18 to $21^{\circ}C$ of temperature, 30 to 33 psu of salinity, 2500 to $5000individual\;m^{-2}$ of rearing density, and feeding supplement of $5{\times}10^4cell\;mL^{-1}$ of I. galbana and T. suecica mixture under semi running water system.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.39 no.1
• /
• pp.49-54
• /
• 2006

Cultured cells of the toxic Alexandrium tamarense were fed to four mussel species, Mytilus coruscus, M. edulis, M. galloprovincialis and Septifer vulgatus, to examine the interspecies and interlocality differences in the ability to accumulate paralytic shellfish poisoning (PSP) toxins. Toxin content of A. tamarense cells varied during culture period. In contrast, toxin composition in the cell (C1,2, GTX1-4 and neoSTX) was constantly stable. In feeding experiment, the four mussel species collected from Geoje intoxicated after uptake of A. tamarense. Toxin content ($average{\pm}SD\;{\mu}g$ STXeq/100 g) of M. coruscus, M. edulis, M. galloprovincialis and Septifer vulgatus were $1,660{\pm}79,\;3,914{\pm}2,242,\;5,626{\pm}1,620\;and\;958{\pm}163$, respectively. Toxin profiles included C1,2, GTX1,4 and neoSTX as the major components, and dcGTX2,3, GTX2,3, neoSTX and STX as the minor ones. Toxin accumulation of three mussel species collected from Pohang, Geoje and Anmyon-do showed interspecies and interlocality differences. Toxin content ($average{\pm}SD\;{\mu}g$ STXeq/100 g) were $91{\pm}4,\;151{\pm}14,\;39{\pm}3$ in M coruscus, $189{\pm}1,\;231{\pm}11,\;206{\pm}15$ in M edu/is and $214{\pm}28,\;326{\pm}30,\;291{\pm}26$ in M. galloprovincialis in order of Anmyon-do, Geoje and Pohang.

• Environmental Analysis Health and Toxicology
• /
• v.21 no.2 s.53
• /
• pp.103-113
• /
• 2006

This study examined effects of crude oil on the phase II drug-metabolizing enzymes UDP-glucuronosyl transferase (UDPGT) and glutathione S-transferase (GST) in mussel Mytilus edulis and rockfish Sebastes schlegeli, a representative bivalve and a culture fish, respectively. This work also intended indirectly to evaluate the post impact recovery from the massive oil tanker spillage accidents occurred during the summer of 1995 in the sea area off Yosu City, Chonnam. For these, enzyme activities of UDPGT and GST were examined in the fish and mussel following laboratory exposure to fresh crude oil, weathered oil, field-obtained oil residues, or in the field biota samples. Decreased GST activity was observed in rock fish following exposure to oil-soluble fraction (OSF) of fresh oil. A similar diminished GST activity was also observed after OSF of artificially weathered oil. OSF of field oil residues retrieved from the spillage area approximately 1 year later also exerted a slight inhibition of GST to rockfish. There was neither a change in UDPGT in rockfish, nor were there changes in mussel in both enzymes to any oil fractions. We could not observe any difference in the two enzymes either in rockfish or mussel sampled from the field during $1.5{\sim}2.0$ years post spillage, indicating that their enzyme systems might had been recovered by the sampling time. In conclusion, it seems that the inhibition of GST activity in rockfish is a biomarker response to crude oil exposure. The results, however, must be interpreted with care, as the inhibition nay reflect various factors such as oil concentration, duration and water temperature.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.5 no.3
• /
• pp.71-75
• /
• 1972

The growth of the mussel, Mytilus edulis, in a salt-field reservoir was investigated at Dangjin, Choongnam, from June to October in 1970. l. Temperature, salinity, specific gravity, and pH of water were $22.5-27.5^{\circ}C,\;28.6-34.8\%$, 1.019-1.024, and 7.6 respectively in the salt-field reservoir from June 20 to July 19. 2. Three species of Gastropoda and seven species of Pelecypoda were collected. 3. The shell height of the mussel group in the highest abundance increased from 200mm($15\~25mm$) on June 15 to 40mm ($20\~50mm$) on September 26 in the salt-field reservoir, whereas the average shell heights of them were 19.80mm (June 15) and 35. 10mm (Sept. 26) respectively. 4. The daily growth of the mussel from July 10 to August 12 was 0.22 mm in shell height and highest during the experimental period. 5. The relationships between shell length (L) and shell height (H), and between shell length (L) and shell breadth (B) of the young mussel are represented by the following equations respectively : H=1.26776L+5.07978 B=35041L+3.05502 6. From the above results, it is suggested that the culture of the mussell, Mytilus edulis, in a salt-field reservoir is possible.

• The Korean Journal of Malacology
• /
• v.32 no.3
• /
• pp.203-209
• /
• 2016

Growth and survival of the hard shelled mussel spat were investigated to confirm the possibe spat culture in seawater pond from September 2014 to April 2015. Also, we measured simultaneously environmental factors including water temperature, salinity, dissolved oxygen, pH, DIN, DIP, chlorophyll a, and abundance and dominant species of phytoplankton in seawater pond every month. Ranges of water temperature and salinity were $4.0-23.4^{\circ}C$ and 18.8-25.2 psu, respectively, which were rather lower than the reported optimal level. Monthly measured survival rates in all the spat cages were over 90%. Concentration of chlorophyll-a and abundance of phytoplankton were very high, and dominant species phytoplankton were cryptomonads and nanoflagellates. These dominant species were considered to be good food organisms for the bivalve spat. The experimental cage stocked 100 individuals per basket ($2,700ind.\;m^{-2}$) hanging in bottom of seawater pond revealed the highest growth in shell height ($7.63{\pm}4.65mm$), but all experimental cages stocked below 200 individuals per basket ($5,400ind.\;m^{-2}$) did not show statistically significant difference. We may expect that seawater pond would be one of the best culture ground for bivalve spats when appropriate measures are available.

• The Korean Journal of Malacology
• /
• v.19 no.1
• /
• pp.25-32
• /
• 2003

To know the effects of the red tide and toxic dinoflagellates on survival and growth of larvae of the mussel, Mytilus galloprovincialis, laboratory experiments were conducted by incubating larvae with either unialgal culture of 4 dinoflagellate species (Amphidinium carterae, Prorocentrum triestinum, Gymnodinium impudicum, or Akashiwo sanguinea) or a standard food (Isochrysis galbana) for 10 days. The survival of larvae was higher than 80% when the food was A. carterae, G. impudicum, or A. sanguinea. The lowest survival (20%) was found when the food was P. triestinum. When the food was P. triestinum, the survival of larvae rapidly decreased from 87% at day 4 down to ca. 50% at day 6, and 20% at day 10. This implies that the larval population of M. galloprovincialis can seriously be affected if they are exposed to the red tide water dominated by P. triestinum for more than 4 days. Shell length of larvae either increased or decreased according to the food species. When the food was A. carterae, G. impudicum, or A. sanguinea, shell length of larvae increased. But, it decreased when the food was P. triestinum. Though shell length increased in 3 treatments, the daily increments (0.63 $\mu$ m for A. carterae, 0.46 m for $\mu$ G. impudicum, and 1.10 m for $\mu$ A. sanguinea) were smaller than that of the standard food (3.79 m for $\mu$ I. galbana). Correlation analyses chowed that the change in shell length was not significant when the food was A. carterae or G. impudicum. Therefore, all of 4 dinoflagellates affected the growth of M. galloprovincialis larvae: growth was negative for P. triestinum, nil for A. carterae and G. impudicum, and positive but lower than standard food for A. sanguinea. These imply that the dinoflagellates are less valuable as foods for M. galloprovincialis larvae. So, decreased growth rate of larvae is expected during red tides, which will consequently cause delayed metamorphosis or failure to recruitment to the adult populations. In considering the harmful effects of red tides on the aquatic ecosystem, not only the effects on adult populations of fish and shellfish, but also the effects on larval populations should be included.

• Journal of Microbiology and Biotechnology
• /
• v.23 no.5
• /
• pp.656-660
• /
• 2013

Pseudomonas fluorescens strain CL0145A was discovered at the New York State Museum Field Research Laboratory as an effective agent against the environmentally destructive zebra mussel, which has contaminated US waters. Dried cells of the microbe are being commercialized as an environmentally friendly solution to the problem. We found that antibiotic activity against the Gram-positive bacterium Bacillus subtilis is produced and excreted by this strain. We have carried out studies to optimize production of the antibiotic. Studies were begun in a complex corn meal medium. Activity was found in both cells and culture supernates and was maximal after one day of fermentation. Static fermentation conditions were found to be superior to shaken culture. Production of extracellular antibiotic in complex medium was found to be dependent on the content of sucrose and enzyme-hydrolyzed casein. Indeed, production was greater in sucrose plus enzyme-hydrolyzed casein than in the complex medium. Of a large number of carbon sources studied as improvements over sucrose, the best was glycerol. An examination of nitrogen sources showed that production was improved by replacement of enzyme-hydrolyzed casein with soy hydrolysates. Production in the simple glycerol-Hy-Soy medium was not improved by addition of an inorganic salt mixture or by complex nitrogen sources, with the exception of malt extract. In an attempt to keep the medium more defined, we studied the effect of amino acids and vitamins as replacements for malt extract. Of 21 amino acids and 7 vitamins, we found tryptophan, glutamine, biotin, and riboflavin to be stimulatory. The final medium contained glycerol, Hy-Soy, tryptophan, glutamine, biotin, and riboflavin.