• Journal of Microbiology and Biotechnology
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• v.20 no.7
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• pp.1069-1076
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• 2010

A novel laccase from Tricholoma mongolicum was purified by using a procedure that entailed ion-exchange chromatographies on DEAE-cellulose, CM-cellulose, and Q-Sepharose, and FPLC-gel filtration on Superdex 75. The purified enzyme was obtained with a specific activity of 1,480 U/mg-protein and a final yield of 15%. It was found to be a monomeric protein with a molecular mass of 66 kDa as estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Its N-terminal amino acid sequence was GIGPVADLYVGNRIL, similar to some but also different to other mushroom laccases. The optimum pH and temperature for the purified enzyme were pH 2 to pH 3 and $30^{\circ}C$, respectively. It displayed a low $K_m$ toward 2,7-azinobis (3-ethylbenzothiazolone-6-sulfonic acid) diammonium salt (ABTS) and high $k_{cat}/K_m$ values. The purified laccase oxidized a wide range of lignin-related phenols, but exerted maximal activity on ABTS. It was significantly inhibited by $Hg^{2+}$ ions, and remarkably stimulated by $Cu^{2+}$ ions. It inhibited HIV-1 reverse transcriptase and proliferation of hepatoma HepG2 cells and breast cancer MCF7 cells with an $IC_{50}$ of 0.65 ${\mu}M$, 1.4 ${\mu}M$, and 4.2 ${\mu}M$, respectively, indicating that it is also an antipathogenic protein.

• Journal of the Korean Wood Science and Technology
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• v.33 no.3 s.131
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• pp.79-88
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• 2005

This research studied the utilization of the bed log wastes as a papermaking grade pulp. Five different bed log samples from shiitake mushroom (Lentinus edodes (Berk.) Sing) cultivation were collected by the cultivating periods of 1 to 5 years. The wood chemical composition and the characteristics of kraft pulping of each sample were investigated. The results of chemical composition showed that the rate of carbohydrate (glucose and xylose) content in sapwood was decreased as the cultivation period was increased. In heartwood, there was no significant difference. The screening yield of non-cultivated bed log from kraft pulping was higher than that of cultivated one, but the reject of cultivated one, especially for 5 year-cultivated, was lower than non-cultivated bed log. The fiber length and width was continuously decreased as the cultivation period was increased. Therefore, the freeness of the pulp from the cultivated bed log was sharply decreased comparing to non-cultivated due to the fiber cutting and the increased fine content. The dry strengths were increased according to the increasing addition level of bed log kraft pulp to KOCC and non-cultivated wood pulp. From the overall results, the pulp from 5 years cultivated bed log can be reasonably used if it is mixed with long fiber pulp for advantages such as reducing beating time.

• Journal of Mushroom
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• v.2 no.1
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• pp.1-3
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• 2004

This study were conducted to investigate pin-heading induction and growing temperature of Agaricus brunescens. The temperature for fruit body formation was better at $15^{\circ}C$ than at 18 and $21^{\circ}C$. As the temperature go up to $21^{\circ}C$, the fruit body number was decreased but the fruit body size and weight was increased. Optimal temperature for both pin-heading and growth of Agaricus brunescens was $18^{\circ}C$.

• The Korean Journal of Mycology
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• v.28 no.2
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• pp.93-96
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• 2000

To investigate the effect of inorganic nutrient supplement in oyster mushroom (Pleurotus ostreatus), we have conducted some study on cultural and growth characteristics of fruitbody formation and chemical composition of media and fruitbody. When supplemented with slow releasing fertilizer, contamination rate was not different from non-supplemented medium, days for incubation time and first pinhead were faster than non supplemented medium. And fruitbody yield and biological efficiency were increased $10{\sim}28%,\;7{\sim}20%$ respectively, but biological efficiency was decreased when increased supplement ratio. The chemical compositions (total carbon, total nitrogen, ammonia nitrogen, nitrate nitrogen, potassium and phosphate) of slow releasing fertilizer supple mented medium and fruitbody were compared with non-supplemented.

• Korean Journal of Agricultural Science
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• v.10 no.1
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• pp.90-96
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• 1983

The short period fermentation of the mushroom compost was studied as a method for the labor saving and mechanization of composting. Rice straw was crushed, watered and supplemented with organic nutrient sources. The straw-nutrient mixtures prepared were stacked and turned for short periods at the phase I and the phase II was carried out for 8 days. The total nitrogen of the short compost was lower than that of the conventional straw compost and it caused the yield decrease. The productivity of the short compost could be improved by the supplement of nurient sources. The effective nutrients were perilla meal and rice bran. Higher microflora of the rice straw-nutrient mixture during fermentation could shorten the composting period. For the short compost method the proper period of the phase I was 5 days.

• Journal of Mushroom
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• v.16 no.4
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• pp.287-292
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• 2018

'Yeoreumhyang1ho' - a new variety of Flammulina velutipes, light brown in color and adaptable to high temperature - was bred by mating two monokaryons isolated from 'Garlmoe' and 'Konkuk1ho'. It exhibited better quality compared to 'Geumhyang' and 'Garlmoe' under conditions where temperature was maintained at $16^{\circ}C$, without exposure to low temperature ($4^{\circ}C$), during the cultivation period. Bottle cultivation at $16^{\circ}C$ required 22, 6, and 10 days for mycelial growth, primordia formation, and growth of the fruit body, respectively. The total cultivation period for 'Yeoreumhyang1ho' was observed to be 38 days, which is 3 and 7 days shorter than that for 'Geumhyang' and 'Garlmoe', respectively. The primordia formation showed remarkable uniformity and the pileus color was observed to be light brown, an intermediate between yellowish ivory of 'Geumhyang' and medium brown of 'Garlmoe'. The yield was 138 g per 850 ml bottle, which was 10% higher than that of 'Geumhyang'.

• Journal of Mushroom
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• v.16 no.4
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• pp.263-266
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• 2018

This study was investigated the growth characteristics of 'Nongjingo' cultivar(Lentinula edodes) according to relative humidity(RH). The color difference of the pileus showed the highest L(Lightness, L) value in RH65 and the a(Redness, a) value in RH95. b(Yellowness, b) values were similar in all treatments. The hardness of pileus is highest at RH95. As the relative humidity increased, the length of pileus and stipe tended to increase. The diameter and thickness of pileus were high at RH95%. The diameter of stipe could not see the big difference in the three treatment groups. This study growth characteristics and yield were increased at higher relative humidity, but quality was decreased. Therefore, must adjust the relative humidity to produce high quality mushrooms.

• Journal of Mushroom
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• v.22 no.2
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• pp.53-59
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• 2024

This study analyzed the effects of different nitrogen sources in substrate composition on the growth of Pleurotus ostreatus, as well as the subsequent changes in flavor and antioxidant activity. The T2, composed of poplar sawdust, beet pulp, cotton seed dregs, and cotton seed coat in a ratio of 40:20:20:20, exhibited the highest yield at 156.6 g. The total polyphenol content and ABTS and DPPH radical scavenging activities were 8.25 mg GAE/g, 70%, and 49%, respectively, showing higher radical scavenging activity compared to the Control and T1. Additionally, varying nitrogen content resulted in distinct aroma patterns and is presumed to influence taste profiles such as sourness, umami, and saltiness.

• Journal of Mushroom
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• v.3 no.4
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• pp.140-144
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• 2005

This study was conducted to verify the cause of suppression symptom in mycelial growth during summer and to be able to establish a countermeasure. Cultivation of Flammulina velutipes was experimented with varying elapsed time of 0, 3, 6, 9 hours after mixing the sawdust media and two kinds of water temperature (normal water, $24^{\circ}C$ and cold water, $6^{\circ}C$) for mixing sawdust media. There were trends of increased media temperature from $24^{\circ}C$ to $31^{\circ}C$ and decreased pH from 6.5 to 5.2~5.6 with varying elapsed time from mixing the media to sterilization. Bacterial density also increased with bacterial density in Medium $24^{\circ}C$ being 1.9~4.1 times higher than that in Medium 6. Growth of F. velutipes was delayed with dual culture of bacteria isolated from sawdust media. Total nitrogen content of sawdust media was lowered by elapsed time from mixing the media to sterilization. The use of normal water($24^{\circ}C$) delayed mushroom growth by 1~2 days compared with that of cold water($6^{\circ}C$). Furthermore, mycelial growth of F. velutipes in the bottle cultivation ceased 9 hours after mixing the media. Primordia formation of F. velutipes was delayed by 1~3 days by elapsed time after mixing sawdust media, while fruit-body yield decreased by 7~12% 6 hours after mixing the media. Fruit-body yield was more stabilized with the use of cold water($6^{\circ}C$) than with that of normal water($24^{\circ}C$). Results showed that it is effective to use cold water as low as $6^{\circ}C$ in mixing media for F. velutipes cultivation, especially during summer when environmental temperature is high, high pressure sterilization after bottling work can prevent bacterial propagation in the media and can stabilize media ingredient.

• The Korean Journal of Mycology
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• v.42 no.1
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• pp.57-63
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• 2014

This study was carried out to obtain morphological and physiological characteristics of ear mushrooms for an artificial cultivation. Eighteen strains were cultivated with bag culture and classified into mainly five groups such as brown, black, white, purple and others group. The highest yield was shown in 43007 strain as 98.3 g/bag and 43009, 43016, 43025 and 44035 strains were more than 60 g/bag. Among collected strains, 43007, 43009 and 43035 were selected in this study as superior strains. Three selected strains were investigated for optimal mycelial growth conditions. MCM and GPYM media were selected for the favorable culture medium. The carbon sources of 43007, 43009 and 43035 on mycelial growth were maltose, fructose and glucose, respectively and peptone was selected as a nitrogen source. Highest mycelial growth was observed when the C/N ratio was 10 for 43007 and 20 for 43009 and 43035.