• Tuberculosis and Respiratory Diseases
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• 제49권2호
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• pp.231-236
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• 2000

10년전 자궁근종으로 자궁전절제술을 시행받은 39세 여자환자로 기침을 주소로 내원하여 흉부 단순촬영, 흉부 전산화 단층촬영상 좌측상엽기관지에 연하여 3$\times$4cm크기 종괴 및 우하부 기저부에 5mm크기의 소결절이 관찰되어, 좌측 상엽 절제술을 시행받았다. 종괴의 병리조직소견상 유사분열을 보이는 세포가 50개의 고배율시야당 2-3개 정도이었으며 세포의 이형성은 관찰할 수 없었고 면역조직화학염색상 Smooth muscle actin, Vimentin, Desmin에 양성반응을 보여 평활근종임을 확인하였다. 수술 후 별다른 증상의 호소는 없었고, 경과 추적 관찰중 우측 폐에 새로운 결절이 발견되어 양성 전이성 평활근종으로 확진하였으며, 이에 문헌고찰과 함께 보고하는 바이다.

• Journal of Periodontal and Implant Science
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• 제36권3호
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• pp.613-625
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• 2006

Periodontal ligament(PDL) cells and human gingival fibroblasts(HGFs) play important roles in development, regeneration, normal function, and pathologic alteration. PDL cells and HGFs have the similarity related with general characteristics of fibroblast such as spindle shaped morphology, the presence of vimentin intermediate filament and the synthesis of interstitial collagens and fibronectin. There were many studies about the differences between PDL cells and HGFs, but they were not about whole gene level. In this study, we tried to explain the differences of gene expression profiles between PDL cells and HGFs, and the differences among three individuals by screening gene expression patterns of PDL cells and HGFs, using cDNA microarray. Although there were some variants among three experiments, a set of genes were consistentely and differentially expressed in one cell type. Among 3,063 genes, 49 genes were more highly expressed in PDL cells and 12 genes were more highly expressed in HGFs. The genes related with cell structure and motility were expressed more highly in PDL cells. These are cofilin 1, proteoglycan 1 secretory granule, collagen type I(${\alpha}$ 1), adducin gamma subunit, collagen type III(${\alpha}$ 1), fibronectin, lumican(keratan sulfate proteoglycan), and ${\alpha}$ -smooth muscle actin. Tissue inhibitor of metalloproteinase known as the enzyme controlling extracellular matrix with matrix metalloproteinase is more highly expressed in PDL cells, osteoprotegerin known as osteoclastogenesis inhibitory factor is more highly expressed in HGFs. We performed northern blot to verify cDNA microarray results on selected genes such as tissue inhibitor of metalloproteinase, fibronectin, osteoprogeterin. The result of northern blot analysis showed that each cell expressed the genes in similar pattern with cDNA microarray result. This result indicates that cDNA microarray is a reliable method in screening of gene expression profiles.