Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
권호 표지

Screening of genes differentially expressed in cultured human periodontal ligament cells and human gingival fibroblasts

배양된 치주인대세포와 치은섬유아세포에서 상이하게 발현된 유전자들의 검토 양상

  • Yoon, Hye-Jeong (Department of Periodontology, college of Dentisrtry, Kyungpook National University) ;
  • Choi, Mi-Hye (Department of Periodontology, college of Dentisrtry, Kyungpook National University) ;
  • Yeo, Shin-II (Department of Periodontology, college of Dentisrtry, Kyungpook National University) ;
  • Park, Jin-Woo (Department of Periodontology, college of Dentisrtry, Kyungpook National University) ;
  • Choi, Byung-Ju (Department of Dental Pharmacology, College of Dentistry, School of Medicine, Kyungpook National University) ;
  • Kim, Moon-Kyu (Department of Immunology, School of Medicine, Kyungpook National University) ;
  • Kim, Jung-Chul (Department of Immunology, School of Medicine, Kyungpook National University) ;
  • Suh, Jo-Young (Department of Periodontology, college of Dentisrtry, Kyungpook National University)
  • 윤혜정 (경북대학교 치과대학 치주과학교실) ;
  • 최미혜 (경북대학교 치과대학 치주과학교실) ;
  • 여신일 (경북대학교 치과대학 치주과학교실) ;
  • 박진우 (경북대학교 치과대학 치주과학교실) ;
  • 최병주 (경북대학교 치과대학 치과약리학교실) ;
  • 김문규 (경북대학교 의과대학 면역학교실3) ;
  • 김정철 (경북대학교 의과대학 면역학교실3) ;
  • 서조영 (경북대학교 치과대학 치주과학교실)
  • Published : 2006.09.30
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Abstract

Periodontal ligament(PDL) cells and human gingival fibroblasts(HGFs) play important roles in development, regeneration, normal function, and pathologic alteration. PDL cells and HGFs have the similarity related with general characteristics of fibroblast such as spindle shaped morphology, the presence of vimentin intermediate filament and the synthesis of interstitial collagens and fibronectin. There were many studies about the differences between PDL cells and HGFs, but they were not about whole gene level. In this study, we tried to explain the differences of gene expression profiles between PDL cells and HGFs, and the differences among three individuals by screening gene expression patterns of PDL cells and HGFs, using cDNA microarray. Although there were some variants among three experiments, a set of genes were consistentely and differentially expressed in one cell type. Among 3,063 genes, 49 genes were more highly expressed in PDL cells and 12 genes were more highly expressed in HGFs. The genes related with cell structure and motility were expressed more highly in PDL cells. These are cofilin 1, proteoglycan 1 secretory granule, collagen type I(${\alpha}$ 1), adducin gamma subunit, collagen type III(${\alpha}$ 1), fibronectin, lumican(keratan sulfate proteoglycan), and ${\alpha}$ -smooth muscle actin. Tissue inhibitor of metalloproteinase known as the enzyme controlling extracellular matrix with matrix metalloproteinase is more highly expressed in PDL cells, osteoprotegerin known as osteoclastogenesis inhibitory factor is more highly expressed in HGFs. We performed northern blot to verify cDNA microarray results on selected genes such as tissue inhibitor of metalloproteinase, fibronectin, osteoprogeterin. The result of northern blot analysis showed that each cell expressed the genes in similar pattern with cDNA microarray result. This result indicates that cDNA microarray is a reliable method in screening of gene expression profiles.

Keywords

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