• Title/Summary/Keyword: muscle growth

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Factors Influencing Satellite Cell Activity during Skeletal Muscle Development in Avian and Mammalian Species

  • Nierobisz, Lidia S;Mozdziak, Paul E
    • Asian-Australasian Journal of Animal Sciences
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    • v.21 no.3
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    • pp.456-464
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    • 2008
  • Avian and mammalian skeletal muscles exhibit a remarkable ability to adjust to physiological stressors induced by growth, exercise, injury and disease. The process of muscle recovery following injury and myonuclear accretion during growth is attributed to a small population of satellite cells located beneath the basal lamina of the myofiber. Several metabolic factors contribute to the activation of satellite cells in response to stress mediated by illness, injury or aging. This review will describe the regenerative properties of satellite cells, the processes of satellite cell activation and highlight the potential role of satellite cells in skeletal muscle growth, tissue engineering and meat production.

cDNA Microarray Analysis of the Gene Expression Profile of Swine Muscle

  • Kim, Chul Wook;Chang, Kyu Tae;Hong, Yeon Hee;Jung, Won Yong;Kwon, Eun Jung;Cho, Kwang Keun;Chung, Ki Hwa;Kim, Byeong Woo;Lee, Jung Gyu;Yeo, Jung Sou;Kang, Yang Su;Joo, Young Kuk
    • Asian-Australasian Journal of Animal Sciences
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    • v.18 no.8
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    • pp.1080-1087
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    • 2005
  • By screening specific genes related to the muscle growth of swine using cDNA microarray technology, a total of 5 novel genes (GF (growth factor) I, II, III, IV and V) were identified. Results of southern blotting to investigate the number of copies of these genes in the genome of swine indicated that GF I, GF III, and GF V existed as one copy and GF II, and GF IV existed as more than two copies. It was suggested that there are many isoforms of these genes in the genome of swine. Also, results of northern blotting to investigate whether these genes were expressed in grown muscle, using GF I, III, and V indicated that all the genes were much more expressed in the muscle of swine with body weight of 90 kg. Expression patterns of these genes in other organs, namely muscle and propagation and fat tissues, were investigated by extracting RNA from the tissues. These genes were not expressed in the propagation and fat tissues, but were expressed in the muscle tissue. To determine the mechanism of muscle growth, further studies should be preceded using the 3 specific genes related to muscle growth, that is GF I, III, and V.

AN EXPERIMENTAL STUDY ON THE MANDIBULAR GROWTH FOLLOWING RESECTION OF THE LATERAL PTERYGOID MUSCLE IN RAT (백서 외측익돌근 제거가 하악골 성장에 미치는 영향에 관한 실험적 연구)

  • Kim, Tae-Woo
    • The korean journal of orthodontics
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    • v.16 no.1
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    • pp.119-131
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    • 1986
  • The purpose of the study is to note the effects of the resection of the lateral pterygoid muscle on the mandibular growth in the growing rats. Twenty four female Wistar rats were used in the experiment. They were divided into three groups: group 1 ; bilateral sham operation group 2 ; bilateral resenction of the lateral pterygoid muscle group 3 ; unilateral resection of the lateral pterygoid muscle (The right lateral pterygoid muscle was resected and the left one was sham-operated.) Groups 1&2 were sacrificed eight weeks lateral and group 3, four weeks later. All specimens were measured with calipers, and stained with hematoxylin and eosin. Groups 1 & 2 were compared with each other. In group 3, the right side was compared with the left. The results were as follows: 1. In the growing rats, the resection of the lateral pterygoid muscle was followed by a decrease of the mandibular growth. After eight weeks, the condyle-mental foramen distance, the angular process-mental foramen distance, the size of the condylar head, the supradentale-first molar distance, and the diameter of the symphysis were significantly smaller than the control. 2. Resection of the lateral pterygoid muscle resulted in decrease of the thickness of the cartilage layer and the prechondroblastic-chondroblastic layer after four weeks. 3. After eight weeks, group 1 and group 2 were not different significantly in the histologic sections. 4. The condylar cartilage was stabilized eight weeks after the experiment.

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Growth- and Breed-related Changes of Fetal Development in Cattle

  • Mao, W.H.;Albrecht, E.;Teuscher, F.;Yang, Q.;Zhao, R.Q.;Wegner, J.
    • Asian-Australasian Journal of Animal Sciences
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    • v.21 no.5
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    • pp.640-647
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    • 2008
  • Breed differences in adult animals are determined during fetal development. If interventions are to be developed that influence growth of muscle and fat, it is important to know at which time during gestation breed differences appear and are fixed. The objective of this study was to characterize fetal development in cattle of different breeds. Pregnant cows of 4 cattle breeds with different growth impetus and muscularity were slaughtered under normal processing conditions and the fetuses were removed. German Angus, a typical beef cattle; Galloway, a smaller, environmentally resistant beef type; Holstein Friesian, a dairy type; and Belgian Blue, an extreme type for muscle growth were used. Fetuses of each breed were investigated at 3, 6, and 9 mo of gestation. Fetuses were weighed and dissected into carcass, organs, and muscles. Body fat weight was obtained using the Soxhlet extraction method. Fetal weight increased most rapidly in the third trimester of gestation mainly due to the accelerated muscle and fat deposition. The organ weight to body weight (BW) ratios decreased and the muscle and fat weight to BW ratios increased. At 3 mo of gestation, Galloway fetuses had the significantly smallest BW, half-carcass weight, leg weight, organ weight, muscle weight and shortest leg length. In contrast, Holstein fetuses had the significantly greatest BW, liver, kidney, and lung weights and significantly longest leg length among the 4 breeds, but no differences between Holstein Friesian and Belgian Blue were detected in half-carcass and leg weight. Indeed, Belgian Blue fetuses had the significantly greatest half-carcass weight, leg weight, and muscle weight at 9 mo of gestation, and Galloway had a significantly greater body fat to BW ratio than Holstein Friesian and Belgian Blue. These differences were not evident at 3 and 6 mo of gestation. These data show that the profound increase of tissue and organ weights occurred in later gestation in cattle fetuses even though breed differences were evident as early as 3 mo of gestation. Depending on the tissue of interest, impacting fetal growth likely needs to occur early in gestation before the appearance of breed-specific differences.

The Effect of Swiss Ball Exercises and Taping Therapy on Back Muscle Strength of Healthy Subjects

  • Kim, Yong-Kwon;Lee, Hyeon-Min;Lee, Mun-Hwan
    • Journal of International Academy of Physical Therapy Research
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    • v.1 no.2
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    • pp.149-154
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    • 2010
  • The purpose of this study was to investigate the effects of swiss ball exercise and taping therapy on back muscle strength on normal college students. The aim of this study was to find effective method for back muscle strengthening. Subjects of 30 college students divided 3 groups(taping therapy group: 10, swiss ball group: 10, control group: 10). All subjects inquired physical conditions and normal exercise habits for data base. Back muscle strength measured before and after 3 weeks intervention. Taping therapy was displayed stable a growth curve in continuative a growth graph of back muscle strength better than swiss ball exercise, because it was taping therapy by periodic effect. The result of this study known to effective either taping therapy or swiss ball exercise, but both taping therapy and swiss ball exercise were effect to increase in back muscle strength.

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Developmental Proteomic Profiling of Porcine Skeletal Muscle during Postnatal Development

  • Kim, Nam-Kuk;Lim, Jong-Hyun;Song, Min-Jin;Kim, Oun-Hyun;Park, Beom-Young;Kim, Myung-Jick;Hwang, In-Ho;Lee, Chang-Soo
    • Asian-Australasian Journal of Animal Sciences
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    • v.20 no.10
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    • pp.1612-1617
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    • 2007
  • In this study, we have compared the skeletal muscle proteome at various stages of porcine postnatal development. Korean native pigs were divided into five postnatal stages of 30, 70, 130, 170 and 300 d and their loin muscles were analyzed for muscle proteome by using two-dimensional electrophoresis and mass spectrometry. We found 5 proteins showing a consistent pattern during skeletal muscle growth. Four proteins were identified as myosin light chain 1 slow-twitch (MLC1sa) isoform, troponin T, triosephosphate isomerase (TIP) and DJ-1 protein. The remaining protein was not identified. Two muscle fiber proteins of MLC1sa isoform and troponin T showed a high expression level at an early postnatal stage and then their levels were decreased markedly during growth stages. On the other hand, the expression of TIP and DJ-1 protein, which are well known as catalysis enzyme and antioxidant-related protein, respectively, were linearly increased during growth stages. Thus, the stage-related muscle proteins may be useful as parameters for understanding the developmental characteristics of biochemical and physiological properties in Korean native pig skeletal muscle.

Genetic Variation in Growth and Body Dimensions of Jersey and Limousin Cross Cattle. 2. Post-Weaning Dry and Wet Season Performance

  • Afolayan, R.A.;Pitchford, W.S.;Weatherly, A.W.;Bottema, C.D.K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.15 no.10
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    • pp.1378-1385
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    • 2002
  • The importance of direct genetic, maternal, heterosis and epistatic effects were examined on post-weaning weight, height, length, girth, fat depth and muscle (ratio of stifle to hip width) with dry and wet season gains in these traits. The breeds used were two pure breeds (Jersey and Limousin), the Limousin${\times}$Jersey $F_1$, and two backcrosses ($F_1{\times}$Jersey dams and $F_1{\times}$Limousin dams). Direct genetic effects were large (p<0.001) for all traits except for length. Jersey maternal effects were large for weight, girth, fat depth and muscle in the post-weaning wet season gains which is an evidence of the impact of Jersey dam on progeny beyond weaning. There were large heterosis effects on fat depth and muscle relative to other traits. Epistatic effects were observed for post-weaning performance in weight, girth, fat depth and muscle. There are indications that there were different genetic effects for post-weaning compared to preweaning growth traits. Thus, it could be hypothesized from this study that different quantitative trait loci (QTL) affect early and late growth in Jersey and Limousin cross cattle breeds. The follow up work will examine the different chromosomal gene effects on pre- and post-weaning growth.

Effect of Shear Stress on Bovine Aortic Smooth Muscle Cell Growth (우 대동맥 평활근 세포의 성장에 관한 shear stress의 영향)

  • 김동욱
    • KSBB Journal
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    • v.11 no.1
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    • pp.52-57
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    • 1996
  • Bovine aortic smooth muscle cells cultured on the slide glass were exposed to sheared flow up to 120 hours in flow chamber to see the effect of shear stress on cell growth in wall shear stresses of 0 to 26dyn/$cm^2$. From lactate dehydrogenase concentration measurement of the circulating medium, it was shown that sheared flow in the shear stress range did not remove additional smooth muscle cells from the slide glass compared with cells in stationary condition. According to smooth muscle cell counting per$cm^2$ of the surface, smooth muscle cells grew fastest in the stationary condition. As the wall shear stress increased, the growth of cells became slower. When the wall shear stress increased over 17dyn/$cm^2$, cell growth was not observed throughout the experiment.

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AN EXPERIMENTAL STUDY OF THE EFFECTS OF REMOVAL OF THE MASTICATORY MUSCLES ON THE GROWTH OF THE MANDIBLE IN THE GUINEA PIG (저작근의 제거가 Guinea Pig의 하악골 성장에 미치는 영향에 관한 실험적 연구)

  • Ryu, Young Kyu;Park, Tae Soo
    • The korean journal of orthodontics
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    • v.11 no.1
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    • pp.25-30
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    • 1981
  • Fifteen young pigs were used in this study. The animals were divided into three groups 1. Group for removal of the temporal muscle, 2. Group for removal of the masseter muscle, 3, Group for removal of masseter and internal pterygoid muscles. The animals were anesthetized with $3.5\%$ chloral hydrate intraperitoneally. In the right side the head was shaved. The masticatory muscle was removed. The animals were sacrificed four months later. The head was separated from trunk and cleaned by boiling in a solution of potassium hydroxide. The results were as follows; 1. In the group for removal of the temporal muscle, the Coronoid process of the mandible was resorbed. 2. In the group for removal of the masseter muscle, there was produced asymmetrical growth of the mandible, attrition of the molar teeth in the control side, and resorption of the mandibular angle. 3. In the group for removal of the Masseter and Internal muscles, the changes were more severe than that of the group for removal of the masseter muscle. The mandibular angle was completely absent. 4. The growth of the bone seems definitely related to the presence of the muscular tissue actively pulling upon it.

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Muscle differentiation induced up-regulation of calcium-related gene expression in quail myoblasts

  • Park, Jeong-Woong;Lee, Jeong Hyo;Kim, Seo Woo;Han, Ji Seon;Kang, Kyung Soo;Kim, Sung-Jo;Park, Tae Sub
    • Asian-Australasian Journal of Animal Sciences
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    • v.31 no.9
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    • pp.1507-1515
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    • 2018
  • Objective: In the poultry industry, the most important economic traits are meat quality and carcass yield. Thus, many studies were conducted to investigate the regulatory pathways during muscle differentiation. To gain insight of muscle differentiation mechanism during growth period, we identified and validated calcium-related genes which were highly expressed during muscle differentiation through mRNA sequencing analysis. Methods: We conducted next-generation-sequencing (NGS) analysis of mRNA from undifferentiated QM7 cells and differentiated QM7 cells (day 1 to day 3 of differentiation periods). Subsequently, we obtained calcium related genes related to muscle differentiation process and examined the expression patterns by quantitative reverse-transcription polymerase chain reaction (qRT-PCR). Results: Through RNA sequencing analysis, we found that the transcription levels of six genes (troponin C1, slow skeletal and cardiac type [TNNC1], myosin light chain 1 [MYL1], MYL3, phospholamban [PLN], caveolin 3 [CAV3], and calsequestrin 2 [CASQ2]) particularly related to calcium regulation were gradually increased according to days of myotube differentiation. Subsequently, we validated the expression patterns of calcium-related genes in quail myoblasts. These results indicated that TNNC1, MYL1, MYL3, PLN, CAV3, CASQ2 responded to differentiation and growth performance in quail muscle. Conclusion: These results indicated that calcium regulation might play a critical role in muscle differentiation. Thus, these findings suggest that further studies would be warranted to investigate the role of calcium ion in muscle differentiation and could provide a useful biomarker for muscle differentiation and growth.