• 한국자원식물학회지
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• 제24권3호
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• pp.267-271
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• 2011

The main objective of the study was to investigate the phytic acid contents of various cereals and legumes produced in Korea. The results showed that the phytic acid contents of buckwheat, foxtail millets, sorghum, millet, barley, jobs' tears, yellow corn and wheat flour (Urimil) were in a range of 0.13 to 2.27%. The contents of the phytic acid ranged from 1.03 to 1.16% for legumes including red Indian bean, black Indian bean, mung bean, and black soybean. The phytic acid content of brown rice was five times higher than those of 100% polished rice. The polishing process of the rice decreased significantly the content of the phytic acid. We estimate that the daily phytic acid intake from rice was changed from 653 mg/day in 1995 to 430 mg/day in 2005 based on the results of a national nutrition survey.

• 대한영양사협회학술지
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• 제27권1호
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• pp.35-44
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• 2021

This study analyzed the intake of calories and nutrients based on the consumption of black beans. The survey was conducted on 124 college students (male: 42, female: 82), of whom 63.7% (79 students) were consumers of black beans. The calorie intake of the black bean intake group (1599.81±555.48 kcal) was significantly higher than that of the non-black bean intake group (1259.99±507.58 kcal) (P<0.01). The black bean intake group also showed a significantly higher intake of crude protein (P<0.05), plant protein (P<0.01), animal protein (P<0.05), crude fiber (P<0.001), plant calcium (P<0.001), plant iron (P<0.001), zinc (P<0.01), vitamin B2 (P<0.01), vitamin C (P<0.01), vitamin E (P<0.01) and folic acid (P<0.001) compared to the non-black bean intake group. There was a positive correlation between the frequency of black bean intake and crude fiber (P<0.05), plant calcium (P<0.05), plant iron (P<0.05), and folic acid (P<0.05). Overall, the black bean intake group was more likely to eat black sesame 13.82 times (95% CI=5.37, 35.55), white beans 10.79 times (95% CI=3.53, 33.02), mung beans 7.22 times (95% CI=2.58, 20.23), and brown rice 4.72 times (95% CI=1.88, 11.84), than the non-black bean intake group. In conclusion, we believe that black beans constitute a vital food ingredient that is necessary to provide Korean college students with a well-balanced diet.

• Journal of Plant Biotechnology
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• 제2권2호
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• pp.61-71
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• 2000

By screening a cDNA library of auxin-treated mung bean (Vigna radiata L.) hypocotyls, we have isolated two full-length cDNA clones, pVR-ACS6 and pVR-ACS7, for 1-aminocyclopropane-1-carboxylate (ACC) synthase, the rate-limiting enzyme in the ethylene biosynthetic pathway. While PVR-ACS6 corresponds to the previously identified PCR fragment pMBA1, pVR-ACS7 is a new cDNA clone. A comparison of deduced amino acid sequences among auxin-induced ACC synthases reveal that these enzymes share a high degree of homology (65-75%) to VR-ACS6 and VR-ACS7 polypeptides, but only about 50% to VR-ACS1 polypeptide. ACS6 and ACS7 are specifically induced by auxin, while ACS1 is induced by cycloheximide, and to lesser extent by excision and auxin treatment. Results from nuclear run-on transcription assay and RNA gel blot studies revealed that all three genes were transcriptionally active displaying unique patterns of induction by IAA and various hormones in etiolated hypocotyls. Particularly, 24-epibrassinolide (BR), an active brassinosteroid, specifically enhanced the expression of VR-ACS7 by distinct temporal induction mechanism compared to that of IAA. In addition, BR synergistically increased the IAA-induced VR-ACS6 and VR-ACS7 transcript levels, while it effectively abolished both the IAA- and kinetin-induced accumulation of VR-ACS1 mRNA. In light-grown plants, VR-ACS1 was induced by IAA in roots, whereas W-ACS6 in epicotyls. IAA- and BR-treatments were not able to increase the VR-ACS7 transcript in the light-grown tissues. These results indicate that the expression of ACC synthase multigene family is regulated by complex hormonal and developmental networks in a gene- and tissue-specific manner in mung bean plants. The VR-ACS7 gene was isolated, and chimeric fusion between the 2.4 kb 5'-upstream region and the $\beta$-glucuronidase (GUS) reporter gene was constructed and introduced into Nicotiana tobacum. Analysis of transgenic tobacco plants revealed the VR-ACS7 promoter-driven GUS activity at a highly localized region of the hypocotyl-root junction of control seedlings, while a marked induction of GUS activity was detected only in the hypocotyl region of the IAA-treated transgenic seedlings where rapid cell elongation occurs. Although there was a modest synergistic effect of BR on the IAA-induced GUS activity, BR alone failed to increase the GUS activity, suggesting that induction of VR-ACS7 occurs via separate signaling pathways in response to IAA and BR.

• Applied Biological Chemistry
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• 제29권3호
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• pp.279-287
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• 1986

발아초기(發芽初期) 녹두(綠豆)의 부위별(部位烈) peroxidase 활성(活性)은 뿌리가 타부위(他部位)보다 약 $1.5{\sim}3.5$배 더 높은 효소활성(酵素活性)을 나타내었으며 발아(發芽)가 진행(進行)됨에 따라 자엽(予葉), 뿌리에서는 효소활성(酵素活性)이 증가(增加)하다가 점차 감소(滅少)하는 경향(傾向)을 보인 반면에 타부위(他部位)에서는 계속 감소(減少)하는 경향(傾向)을 보였다. 전기영동상(電氣泳動上) isozyme의 수는 발아(發芽), 생육(生育)이 진행(進行)됨에 따라 모든 부위(部位)에서 계속 증가(增加)하여 활성도(活性度)와는 무관(無關)하게 발아(發芽) 후 6일(日)째에 가장 많이 나타났다. Ammonium sulfate에 의한 단백질(蛋白質)의 분획(分劃), Sephadex G-75 Column, DEAE-cellulose column한 결과(結果) isozyme A는 16배 정제(精製)되었으며, isozyme B는 조금도 순도(純度)가 증가(增加)되지 않았으며 isozyme A와 B의 전기영동상(電氣泳動上) Rm값은 각각(各各) 0.22와 0.62였다. isozyme A와 B 모두 PH5.6에서 최대(最大) 활성(活性)을 나타내었으며 또한 반응최적온도(反應最適溫度)는 각각(各各) $65^{\circ}C$와 $70^{\circ}C$였으며, 열(熱)에 대해서도 비교적(比較的) 안정(安定)한 결과(結果)를 얻었다. Lineweaver-Burk Polt를 이용(利用)하며 Km값을 측정(測定)해 본 결과(結果) 0-dianisidine에 대한 isozyme A는 0.071mM, isozyme B는 0.052mM이었으며 $H_2O_2$에 대한 isozyme A는 0.28mM, isozyme B는 0.23mM이었다.

• 한국식품저장유통학회지
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• 제16권5호
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• pp.726-733
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• 2009

본 연구에서는 6종류의 종실에서 기름을 착유하여 조지방 함량(crude fat content) 측정 및 총 지방산 조성과 위치별 지방산 조성을 비교 분석 하였으며, triacylglycerol(TAG)의 조성과 tocopherol 함량을 분석하였다. Folch법을 이용하여 분석된 조지방 함량은 들깨 21.64%, 홍화씨 13.85%, 고추씨 9.60%, 석류씨 8.85%, 녹두 2.25%, 결명자 2.00%로 나타났다. 추출된 종실유의 지방산 분석결과 불포화 지방산인 linoleic acid(C18:2)가 81.57 wt%~46.17 wt%로 가장 높은 비중을 차지하였으며, sn-2 위치의 지방산 조성에서도 linoleic acid의 함량이 88.30 wt%~15.99 wt%로 높은 함량을 나타내었다. Triacylglycerol(TAG)의 조성은 reversed-phase HPLC를 이용하여 분석하였으며 partition number(PN)=36~48의 분포를 보였다. Total tocopherol의 함량은 석류씨에서 가장 높은 377.74 mg /100 g으로 나타났으며 이 외에 각각 녹두(141.16 mg/100 g), 결명자(107.23 mg/100 g), 고추씨(33.88 mg/100 g), 들깨(30.05 mg/100 g), 홍화씨(29.80 mg/100 g) 순으로 측정되었다.

• 한국환경농학회지
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• 제17권2호
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• pp.122-126
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• 1998

작물들의 염분에 대한 반응을 알아보기 위해 200mM의 NaCl이 들어있는 영양액을 공급하는 사경재배를 통하여 작물들의 내염성을 비교하였고,간척지 토양에 파종하여 작물들의 출현율과 초기생육을 관찰하였다. 작물종 중 내염성이 강한 종은 목화, 보리, 밀, 옥수수 등으로 이들은 200mM의 NaCl이 함유된 영양액에서 고사하지 않고 생육이 가능하다. 그러나 녹두, 완두, 팥, 콩과 갚은 콩과작물이 염분에 특히 약하였다. 작물을 간척지 토양에 파종했을때 건물중의 피해가 가장 심하였고, 출현율, 초장 순으로 염분에 대한 반응 정도가 작았다. 또한 염분 정도가 증가함에 따라 작물체의 엽수도 감소하였다. 공시 작물들의 초장이 50% 저해 되는 염분 농도를 추정해 볼 때 화본과의 벼, 보리, 옥수수는 각각 22.6, 56.9, 40.4ds/m의 EC에서 초장 50% 저해되었고, 콩과 작물인 녹두와 콩은 각각 22.6과 21.7ds/md의 EC에서 초장이 50% 감소됨을 추정할 수 있었다.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2003년도 생물공학의 동향(XII)
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• pp.361-362
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• 2003

본 실험은 생물비료인 인산가용화균 Penicillium sp. PS-113의 어독성과 주요작물에 대한 유해성을 조사하였다. 어독성 실험의 모든 시험구에서 무해한 것으로 판정되었고, 작물생육효과에서도 매우 뛰어난 효과가 있음을 확인할 수 있었다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 추계학술대회
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• pp.272-272
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• 2017

• 한국동물학회:학술대회논문집
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• 한국동물학회 2001년도 한국생물과학협회 학술발표대회
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• pp.227.2-227
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• 2001

No Abstract, See Full Text

• 대한가정학회지
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• 제19권1호
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• pp.25-31
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• 1981

The changes of various nitrogenfractions and free amino acid composition were investigated at various growing stages. The results are summarized as follows 1) Total nitrogen, water soluble protein nitrogen, and true protein nitrogen were decreased with growth. 2) Free amino acid increased with sprout-growth. the content of free amino acid were about 74.4%(combined Ser, Asp, Arg, Val) after 4 day sprout. It is belived that serine, aspartic acid, arginine and valine play an important role as taste compounds in mungbean sprout. 3) 16kinds of amino acid, including essential amino acids in human nutrition except tryptophan and cystin were quantified.