• Journal of Korean Neurosurgical Society
• /
• 제53권2호
• /
• pp.72-76
• /
• 2013

Objective : Cerebral aneurysm (CA) is an important acquired cerebrovascular disease that can cause catastrophic results. MicroRNAs (miRNAs) are small non-coding RNAs, playing essential roles in modulating basic physiologic and pathological processes. Currently, evidences have been established about biologic relationship between miRNAs and abdominal aortic aneurysms. However, biologic roles of miRNAs in CA formation have not been explained yet. We employed microarray analysis to detect and compare miRNA expression profiles in late stage of CA in rat model. Methods : Twenty-six, 7-week-old male Sprague-Dawley rats underwent a CA induction procedure. The control animals (n=11) were fed a normal diet, and the experimental animals (n=26) were fed a normal diet with 1% normal saline for 3 months. Then, the rats were sacrificed, their cerebral arteries were dissected, and the five regions of aneurysmal dilation on the left posterior communicating artery were cut for miRNA microarrays analysis. Six miRNAs (miRNA-1, miRNA-223, miRNA-24-1-5p, miRNA-551b, miRNA-433, and miRNA-489) were randomly chosen for validation using real-time quantitative PCR. Results : Among a set of differentially expressed miRNAs, 14 miRNAs were over-expressed more than 200% and 6 miRNAs were down-expressed lower than 50% in the CA tissues. Conclusion : The results show that miRNAs might take part in CA formation probably by affecting multiple target genes and signaling pathways. Further investigations to identify the exact roles of these miRNAs in CA formation are required.

• Biomolecules & Therapeutics
• /
• 제23권6호
• /
• pp.549-556
• /
• 2015

Consumption of herbal tea [flower buds of Cleistocalyx operculatus (Roxb.) Merr. et Perry (Myrtaceae)] is associated with health beneficial effects against multiple diseases including diabetes, asthma, and inflammatory bowel disease. Emerging evidences have reported that High mobility group box 1 (HMGB1) is considered as a key "late" proinflammatory factor by its unique secretion pattern in aforementioned diseases. Dimethyl cardamonin (2',4'-dihydroxy-6'-methoxy-3',5'-dimethylchalcone, DMC) is a major ingredient of C. operculatus flower buds. In this study, the anti-inflammatory effects of DMC and its underlying molecular mechanisms were investigated on lipopolysaccharide (LPS)-induced macrophages. DMC notably suppressed the mRNA expressions of TNF-${\alpha}$, IL-$1{\beta}$, IL-6, and HMGB1, and also markedly decreased their productions in a time- and dose-dependent manner. Intriguingly, DMC could notably reduce LPS-stimulated HMGB1 secretion and its nucleo-cytoplasmic translocation. Furthermore, DMC dose-dependently inhibited the activation of phosphatidylinositol 3-kinase (PI3K), phosphoinositide-dependent kinase 1 (PDK1), and protein kinase C alpha (PKC${\alpha}$). All these data demonstrated that DMC had anti-inflammatory effects through reducing both early (TNF-${\alpha}$, IL-$1{\beta}$, and IL-6) and late (HMGB1) cytokines expressions via interfering with the PI3K-PDK1-PKC${\alpha}$ signaling pathway.

• 한국의료질향상학회지
• /
• 제12권1호
• /
• pp.52-61
• /
• 2006

Background : The promotion and advertisement of pharmaceuticals should be based on evidence from clinical trials. We conducted this study to assess whether the pharmaceutical advertisement claims in Korean medical journals had relevant references, and whether the claims were supported by the references. Methods : We reviewed pharmaceutical advertisements in five Korean medical journals issued during the first half of 1999 and during the first half of 2004. Three investigators independently reviewed the advertisements to see whether the studies quoted to endorse the advertising messages supported the corresponding claims. Using multiple logistic regression analyses, we investigated which factors were associated with the quality of the advertisement claims. Results : From the 550 advertisements in the five journals, we identified 157 different advertisements and 475 different promotional claims. Only 149 claims had at least one reference, and 105 claims had references of published article. We could find supporting evidences in the 90 claims. The factors which were associated with the quality of advertisement claims were category of drugs, category of claims, and the manufacturer characteristics. Claims for cardiovascular and endocrine drugs, and claims on efficacy, and claims of multinational company were more evidence-based. Conclusion : Majority of the pharmaceutical advertisement claims in Korea did not have appropriate references. Drug category, claim category, and the manufacturer characteristics were associated with the quality of advertisement claims, and the manufacturer characteristics was the most important determinants.

• 한국콘텐츠학회:학술대회논문집
• /
• 한국콘텐츠학회 2007년도 추계 종합학술대회 논문집
• /
• pp.107-111
• /
• 2007

학술 정보에서 저자는, 실세계의 한 저자가 형태적으로 둘 이상의 저자명으로 출현할 수 있으며, 서로 다른 저자들이 동일한 저자명을 공유하기도 한다. 이는 각각 학술정보에 대한 검색 및 탐색에 있어, 재현율과 정확률을 저하시키는 요인이다. 이 연구에서는 후자에 해당하는 저자의 동명이인 문제에 있어, 그 중의성 해소를 위한 자질의 특성에 집중하고자 한다. 최근까지, 저자 식별을 위한 자질로, 공저자, 논문 제목, 게재지명과 같은 서지 내적 자질과, 논문 원문 텍스트로부터 획득되는 전자메일주소, 소속기관, 논문의 토픽 등과 같은 서지 외적 자질이 사용되어 왔다. 그러나, 이러한 자질들이 저자 식별에 미치는 영향에 대한 비교 분석 연구는 찾아보기 힘들다. 이 연구에서는, 한글 저자명에 대해 원문과 연계된 대용량 저자 식별 평가 셋을 구축하여, 동명 저자 중의성 해소에 있어 다양한 자질들의 특성을 비교한다.

• The Korean Journal of Physiology
• /
• 제6권2호
• /
• pp.49-56
• /
• 1972

This experiment was designed to explore specific functional relationship between the vestibular canals and the extraocular oblique muscles by observing the isometric tension responeses of the muscles to the selected vestibular canal excitation. The vestibular excitation was simulated by either stimulation of the individual canal nerve or endolymphatic fluid displacement in each canal. Each canal nerve was subjected to square wave pulses with a monopolar wire electrode placed closely to the ampullary nerve endings for electrical stimulation, and a fine stainless cannula was introduced into the each canal toward the ampulla and a minute amount $(0.5{\sim}3.5\;microliter)$ of fluid was injected in or ejected out by means of a microsyringe connected to the cannula to produce ampullopetal or ampullofugal displacement of endolymphatic fluid. The superior oblique muscle was contracted by the excitation of homolateral canals and was relaxed by contralateral canals. On the contrary, the inferior oblique was contracted by the contralateral canals and was relaxed by the homolateral canals. Summation of excitatory and inhibitory canal effects from the bilateral vestibular system was demonstrable on the tension changes of the oblique muscles. Excitation of either dual or triple canals of the unilateral vestibular system also caused summation effect on the tension response of the oblique pair; thus multiple signals from the different ampullary receptors seems to be converged into the relevant ocular motor muclei. Since the superior and inferior obliques are known to receive their motor fibers from the contralateral trochlear nuclei and intermediate nuclei of the homolateral oculomotor complex respectively, the above experimental evidences indicate that the ocular motor nuclei for oblique muscles receive excitatory signals from the contralateral vestibular canals and inhibitory signals from the homolateral canals.

• 대한가정학회지
• /
• 제38권8호
• /
• pp.69-84
• /
• 2000

This study intended to investigate the relationship between father's/mother's values(materialism, competitive achievement pressure, neighborhood relations), father/mother-student emotional intimacy & father's/mother's over control and university student's alienation, and examin the relative influences of independent variables on university student's alienation. For this purpose, data were obtained from 402 male 7nd female university students in Seoul and Inchon, and analyzed by factor analysis, Cronbach's $\alpha$, frequencies, %, Pearson's r and stepwise multiple regression. The major findings were as follows : 1) father's and mother's values had significant relations to university student's alienation. As father and mother had more materialism and competitive achievement pressure, their university student was more alienated, and as father and mother had more neighborhood relations, the student was less alienated. 2) Emotional intimacy between father/mother and student had negative relation to university student's alienation. As the level of emotional intimacy was higher, the student was less alienated 3) Father's and mother's over control had positive relations to university student's alienation. As father's and mother's over control was higher, the student was more alienated. 4) Mother's neighborhood relations, father's and mother's over control, mother-student emotional intimacy and father's materialism had influences on university student's alienation in descending order. These independent variables explained 25. 0 % of the total valiance. Father's materialism and father's/mother's over control increased the level of university student's alienation, but mother's neighborhood relations and mother-student emotional intimacy decreased the bevel of student's alienation. Emerging evidences suggested that in order to prevent or decrease the university student's alienation, their parents should have less materialism and more neighborhood relations, and should not use over control on their university student but produce more emotional support.

• 대한의생명과학회지
• /
• 제5권1호
• /
• pp.27-40
• /
• 1999

본 연구는 MarA와 함께 MarB가 AcrAB efflux pump를 목표로 하는지 여부, 그리고 항생제의 균체외 배출기능에 대하여 어떤 조절 작용이 있는지를 항생제 내성검사를 통하여 살펴보았다. 본 연구 결과는 MarB가 MarA와 함께 AcrAB/TolC efflux pump의 항생제 배출기 능을 억제적으로 조절한다는 것을 간접적으로 보여 주었으며, 한편 이미 알려진 대로 MarA는 acrRAB operon의 발현을 전사 수준에 서 positive regulation하므로, MarB는 AcrAB efflux pump의 기능을 억제적으로 조절한다는 것을 암시하고 있다. 그리고 MarA와 함께 MarB단백질의 작용 목표는 AcrAB efflux pump임을 간접적으로 보여주고 있다. 또한 MarB는 MarA와 함께 대장균의 다른 efflux system(들)의 항생제 배출기능에 대해서도 억제적으로 조절할 가능성이 높은 것으로 나타났다.

• Plant Resources
• /
• 제4권3호
• /
• pp.161-170
• /
• 2001

A major portion of drugs used in Ayurvedic system of medicine which has been practiced since the early human civilization in Indian subcontinent were of plant origin. It should be noted that 70% of the population in this region depends on Ayurveda for their medical treatment and 60% of the drug resources are obtained from the Himalayan region. Therefore, Nepal becomes a potential source of plant drug resource since it occupies a major portion of the Himalaya. In the present paper, in general a current status of medicinal plant resources of Himalayan region especially Nepal will be discussed. In addition to this, a typical example of antidiabetic activity of Shilajit will be taken for the discussion. Shilajit is one of the crucial elements in several formulations including those of Rasayana, a therapy in Ayurveda, which has been practiced in the prevention of ageing and mental disorder. Although, Shilajit is widely used for the treatment of diabetes, no satisfactory scientific reports are available up to now. The crude Shilajit in the market is a dark brown or black rock-like substance collected from the Himalayan region with a strong smell of cow's stale urine. In our studies, Shilajit (collected in the central Himalayan region) prevented the diabetes in nonobese diabetic (NOD) mice model. Shilajit also prevented the diabetes in the rats against the action of multiple low-dose (10 mg/kg, i.v., 5 times) of streptozotocin. On the other hand, Shilajit did not show antioxidative activity. The preventive action of Shilajit on diabetes is mainly focused on the Thl and Th2 cell activities, since Th2 cells activity was found to be significantly upregulated. Shilajit, however, showed a mild action in controlling the blood sugar level in young, old, and mild diabetic rats, but not in the severe diabetic rats. It also stimulated the nitric oxide production in macrophages. Based on these evidences, the antidiabetic activities of Shilajit appear to be immunomodulative probably by protecting or strengthening insulin-producing b-cells in the pancreas. Further systematic research on constituents of Shilajit and its quality evaluation is necessary to enable the use of natural medicines in the treatment of diabetes.

• 동의생리병리학회지
• /
• 제26권4호
• /
• pp.432-436
• /
• 2012

Among a number of factors related to the effect of acupuncture and moxibustion treatment, prescriptions do important work. Traditional eastern doctors also placed emphasis on prescriptions of acupuncture and moxibustion, and a lot of experiences have been accumulated. The method forms prescriptions of Sa-am acupuncture therapy pivots on five transport points. Studies show prescriptions are made on the basis of the theory of 'Wang Xiang Xiu Qiu Si'(旺相休囚死) used engendering and restraining of five phases, and it is considered the principle of transformed prescriptions through this theory. Because there is still no study analyzing prescriptions of Sa-am acupuncture by existing principles, we report opinion about Combination principle of points in them. 1. We studied history of acupuncture and moxibustion, development and kinds of points' Combination principle. 2. Around Combination principles of points in Classic of Difficult Issues and Internal Classic, we analyzed and compared between Combination principles of points in Sa-am acupuncture therapy and them in Korea and overall acupuncture methods. 1. There were many Combination principles of acupunture point combination in Classic of Difficult Issues, Internal Classic and Korea acupuncture methods. For example, Point combination of the same meridian, Point combination of the different meridian, Exterior-Interior point combination, Point combination of the same name meridian, Point combination of the multiple meridian, Point combination of Adjacent and Distant points, Left-Right point combination, Anterior-Posterior point combination, Superior-Inferior point combination, Brook point and Stream point combination, Triple energizer point combination, Eight meeting point combination, Five transport point combination, Source-Connecting point combination, Alram point and Transport point combination, Mother-Sun meridian combination, Empirical point combination, Important point combination, Point combination according to syndrome. 2. Existing Combination principles of points in prescriptions of Sa-am acupuncture therapy included every rule. 3. They could be evidences that prescriptions of Sa-am acupuncture therapy are forms inheriting and developing acupuncture and moxibustion treatments.

• Steel and Composite Structures
• /
• 제26권1호
• /
• pp.79-87
• /
• 2018

In order to study the natural vibration characteristics of steel-concrete composite truss beam (SCCTB), the influence of multiple factors such as interface slip, shear deformation and moment of inertia are considered. Afterwards, based on the Hamilton principle the vibration control differential equation and natural boundary conditions of SCCTB are deduced. By solving SCCTB differential equations of vibration control, an analytical calculation method is proposed for analyzing the natural vibration characteristics of SCCTB. The natural frequencies of SCCTBs with different degrees of shear connection and effective lengths are calculated by using the analytical method, and the results are compared against those obtained from ANSYS finite element numerical calculation method. The results show that the analytical method considering the influence factors such as interface slip, shear deformation and moment of inertia are in good agreement with those obtained from ANSYS finite element numerical calculation method. This evidences the correctness of the analytical method and show that the method proposed exhibits improvement over the previously developed theories for the natural vibration characteristics of SCCTB. Finally, based on the analytical method, the influence factors of SCCTB natural vibration characteristics are analyzed. The results indicate that the influence of interface slip stiffness on SCCTB's natural frequency is more than 10% and therefore cannot be neglected. Moreover, shear deformation has an effect of more than 35% on SCCTB's natural frequency and the effect cannot be ignored either in this case too.