Browse > Article
http://dx.doi.org/10.3340/jkns.2013.53.2.72

Dysregulated Expression Profiles of MicroRNAs of Experimentally Induced Cerebral Aneurysms in Rats  

Lee, Hyung-Jin (Department of Neurosurgery, Clinical Research Institute, The Catholic University of Korea)
Yi, Jin-Seok (Department of Neurosurgery, Clinical Research Institute, The Catholic University of Korea)
Lee, Hong-Jae (Department of Neurosurgery, Clinical Research Institute, The Catholic University of Korea)
Lee, Il-Woo (Department of Neurosurgery, Clinical Research Institute, The Catholic University of Korea)
Park, Ki-Cheol (Department of Neurosurgery, Daejeon St. Mary's Hospital, College of Medicine, The Catholic University of Korea)
Yang, Ji-Ho (Department of Neurosurgery, Clinical Research Institute, The Catholic University of Korea)
Publication Information
Journal of Korean Neurosurgical Society / v.53, no.2, 2013 , pp. 72-76 More about this Journal
Abstract
Objective : Cerebral aneurysm (CA) is an important acquired cerebrovascular disease that can cause catastrophic results. MicroRNAs (miRNAs) are small non-coding RNAs, playing essential roles in modulating basic physiologic and pathological processes. Currently, evidences have been established about biologic relationship between miRNAs and abdominal aortic aneurysms. However, biologic roles of miRNAs in CA formation have not been explained yet. We employed microarray analysis to detect and compare miRNA expression profiles in late stage of CA in rat model. Methods : Twenty-six, 7-week-old male Sprague-Dawley rats underwent a CA induction procedure. The control animals (n=11) were fed a normal diet, and the experimental animals (n=26) were fed a normal diet with 1% normal saline for 3 months. Then, the rats were sacrificed, their cerebral arteries were dissected, and the five regions of aneurysmal dilation on the left posterior communicating artery were cut for miRNA microarrays analysis. Six miRNAs (miRNA-1, miRNA-223, miRNA-24-1-5p, miRNA-551b, miRNA-433, and miRNA-489) were randomly chosen for validation using real-time quantitative PCR. Results : Among a set of differentially expressed miRNAs, 14 miRNAs were over-expressed more than 200% and 6 miRNAs were down-expressed lower than 50% in the CA tissues. Conclusion : The results show that miRNAs might take part in CA formation probably by affecting multiple target genes and signaling pathways. Further investigations to identify the exact roles of these miRNAs in CA formation are required.
Keywords
Intracranial aneurysm; MicroRNAs; Cell proliferation; Apoptosis; Inflammation;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Liu G, Huang Y, Lu X, Lu M, Huang X, Li W, et al. : Identification and characteristics of microRNAs with altered expression patterns in a rat model of abdominal aortic aneurysms. Tohoku J Exp Med 222 : 187-193, 2010   DOI
2 Maegdefessel L, Azuma J, Toh R, Deng A, Merk DR, Raiesdana A, et al. : MicroRNA-21 blocks abdominal aortic aneurysm development and nicotine-augmented expansion. Sci Transl Med 4 : 122ra22, 2012
3 Mishra PK, Metreveli N, Tyagi SC : MMP-9 gene ablation and TIMP-4 mitigate PAR-1-mediated cardiomyocyte dysfunction : a plausible role of dicer and miRNA. Cell Biochem Biophys 57 : 67-76, 2010   DOI
4 Park SH, Yim MB, Lee CY, Kim E, Son EI : Intracranial fusiform aneurysms : it's pathogenesis, clinical characteristics and managements. J Korean Neurosurg Soc 44 : 116-123, 2008   DOI
5 Wang XF, Shi ZM, Wang XR, Cao L, Wang YY, Zhang JX, et al. : MiR-181d acts as a tumor suppressor in glioma by targeting K-ras and Bcl-2. J Cancer Res Clin Oncol 138 : 573-584, 2012   DOI
6 Zhuang G, Meng C, Guo X, Cheruku PS, Shi L, Xu H, et al. : A novel regulator of macrophage activation : miR-223 in obesity-associated adipose tissue inflammation. Circulation 125 : 2892-2903, 2012   DOI
7 Chan MC, Hilyard AC, Wu C, Davis BN, Hill NS, Lal A, et al. : Molecular basis for antagonism between PDGF and the TGFbeta family of signalling pathways by control of miR-24 expression. EMBO J 29 : 559-573, 2010   DOI
8 Aoki T, Kataoka H, Moriwaki T, Nozaki K, Hashimoto N : Role of TIMP-1 and TIMP-2 in the progression of cerebral aneurysms. Stroke 38 : 2337-2345, 2007   DOI
9 Aoki T, Moriwaki T, Takagi Y, Kataoka H, Yang J, Nozaki K, et al. : The efficacy of apolipoprotein E deficiency in cerebral aneurysm formation. Int J Mol Med 21 : 453-439, 2008
10 Bartel DP : MicroRNAs : genomics, biogenesis, mechanism, and function. Cell 116 : 281-297, 2004   DOI
11 Guo F, Li Z, Song L, Han T, Feng Q, Guo Y, et al. : Increased apoptosis and cysteinyl aspartate specific protease-3 gene expression in human intracranial aneurysm. J Clin Neurosci 14 : 550-555, 2007   DOI
12 Cheung TH, Quach NL, Charville GW, Liu L, Park L, Edalati A, et al. : Maintenance of muscle stem-cell quiescence by microRNA-489. Nature 482 : 524-528, 2012   DOI
13 Choi YM, Yi JS, Lee HJ, Yang JH, Lee IW : Apolipoprotein E expression in experimentally induced intracranial aneurysms of rats. J Korean Neurosurg Soc 39 : 46-51, 2006
14 Garzon R, Heaphy CE, Havelange V, Fabbri M, Volinia S, Tsao T, et al. : MicroRNA 29b functions in acute myeloid leukemia. Blood 114 : 5331-5341, 2009   DOI
15 Gurha P, Abreu-Goodger C, Wang T, Ramirez MO, Drumond AL, van Dongen S, et al. : Targeted deletion of microRNA-22 promotes stress-induced cardiac dilation and contractile dysfunction. Circulation 125 : 2751-2761, 2012   DOI
16 Johnnidis JB, Harris MH, Wheeler RT, Stehling-Sun S, Lam MH, Kirak O, et al. : Regulation of progenitor cell proliferation and granulocyte function by microRNA-223. Nature 451 : 1125-1129, 2008   DOI
17 Kanematsu Y, Kanematsu M, Kurihara C, Tada Y, Tsou TL, van Rooijen N, et al. : Critical roles of macrophages in the formation of intracranial aneurysm. Stroke 42 : 173-178, 2011   DOI