• 수완나부미
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• 제7권1호
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• pp.139-155
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• 2015

The relation of culture and development is complicated and multilayered. Inclusive development has been the national strategy of the government of President Benigno Aquino III. However, the role the culture is scarcely mentioned. This paper will try to contextualize development in Philippine history to further show the importance of national consciousness, ethnic cultures, indigenous cultures and modern culture,. This paper concludes that inclusive development of the Philippines will be achieved through glocalization, based on the creative reconstruction of cultures in and out of the Philippines.

• Journal of Microbiology and Biotechnology
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• 제25권9호
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• pp.1547-1554
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• 2015

The potential of microalgae biofuel has not been realized because of the low productivity and high costs associated with the current cultivation systems. In this study, a new low-cost and transparent attachment material was tested for cultivation of a filamentous algal strain, Stigeoclonium sp., isolated from wastewater. Initially, the different materials tested for Stigeoclonium cultivation in untreated wastewater were nylon mesh, polyethylene mesh, polypropylene bundle (PB), polycarbonate plate, and viscose rayon. Among the materials tested, PB led to a firm attachment, high biomass (53.22 g/m², dry cell weight), and total lipid yield (5.8 g/m²) with no perceivable change in FAME profile. The Stigeoclonium-dominated biofilm consisted of bacteria and extracellular polysaccharide, which helped in biofilm formation and for effective wastewater treatment (viz., removal efficiency of total nitrogen and total phosphorus corresponded to ~38% and ~90%, respectively). PB also demonstrated high yields under multilayered cultivation in a single reactor treating wastewater. Hence, this system has several advantages over traditional suspended and attached systems, with possibility of increasing areal productivity three times using Stigeoclonium sp. Therefore, multilayered attached growth algal cultivation systems seem to be the future cultivation model for large-scale biodiesel production and wastewater treatment.

• The Korean Journal of Physiology
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• 제28권2호
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• pp.233-240
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• 1994

In this study, it was investigated whether immortalized proximal tubule cells transformed with pRSVT could survive through the numerous passages. Results were as follows: 1. The cells transfected with pRSVT formed rapidly growing, multilayered colonies within 2 weeks in a hormone defined medium. Domes were also observed in some of the cultures. 2. r-glutamyl transpeptidase activity was equivalent to that observed in primary renal proximal tubule cell cultures. 3. Transformed cells with pRSVT form tubules in matrigel following 20 passages. 4. Genomic DNA of transformants was digested with either the restriction enzyme Xba or BamH1. A band of approximately 7.5kb was detected with Xba. Three BamH1 bands were detected at approximately 15 kb, 6.5 kb, and 3 kb.

• 한국조경학회지
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• 제31권2호
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• pp.12-27
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• 2003

The purpose of this study is to look for an environmental design method based on open narrative structure, and to promote various experiences and interpretations of space through user's engagements. That is to say, the designer does not lead specific events through separate Boning but using the continuous open composition users are provided with margins for their imaginations. Spatial formulation through open narrative structure gives us various thoughts and it plays an important role in making a sequential space. Like an abstract painting, it is a complex story making or arranging a montage of images containing stories that elicit the reader's engagement through diverse interpretations. Like this, open composition exists in an ambiguous state and it is possible to interpret unfinished‘evolving work’within it. Utilizing open narrative structure, this study attempts to apply the idea of sequencing and open composition in the case of designing Arirang Culture Park. Open composition should induce various engagements by users and could be a medium which organically connects nature, culture and people. The spatial strategies of‘ambiguity’ and‘transparency’are like a bundle of complex and heterogeneous factors. Finally, the study focuses on the ‘integration’of the main ideas that compose multilayered space. ‘Voidness’and‘thickening’are also used for spatial strategies in open narrative structure. As alternative plans for undecided programs of the space, the voidness can be a strategic design program with flexibility about changes of futures. Also, thickening can be a strategic design program for functional reinforcement of the space, for the dramatic effects and for the generation of incidental events. Although both voidness and thickening seem paradoxical, we can see they are similar in the way that both focus on various spatial uses and by how they do not function as one-to-one correspondence, but as multiple correspondences. Therefore, open narrative structure is possible to apply in designing space and it can be an alternative design strategy for inducing multiple interpretations of space.

• 복식
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• 제51권2호
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• pp.149-167
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• 2001

Today, every culture has taken on the compromise form by means of the cultural difference, variety, and pluralism according to the internationalization and the advance so that it has been developed toward the half-blooded and multilayered the aspect. In accordance with this current of the times, this thesis observed the feminism found in the multiculturism of the end of the 20th century, the third world, and technology with the side of the tendency of modern fashion, considered the hybride phenomenon which is pulling down the wall between culture and genre due to the social diversification. and predicted the fashion trend do 21st century serf on it. Multiculturism is the movement that began to arise in the academic world of America and the literary world form the close of 1980's in accepting the variety of culture and regarding the culture with the more balanced and wide view and just as it is, it means the attitude of accepting one or more cultures of variely and the position of taking interest in the culture of minority race not the culture of a governing race. It is the fashion of feminism adapts dualism like unisex, androgynous look, etc of bisexual lendency in the 1980's, it shows new style with crossover of liberal sense because there is not the difference of sex in fashion. The eco-feminism pursues the natural sexuality not being instrumental and dismantling and expressed it in the Gender expression of an integrated human being. The trend of ethnic fashion in the close of 20th century is that the element of hippie is working so strongly. By adding embroidery of Oriental style, accessories of Indian style, feathers, beads, a hempen hood to the ethnic costumes of Asia and Latin, is shows the figure of ethnic hippie. As the cycler fashion is the future clothes through technology of computer, it uses a cool glass material bringing up the image of a spacesuit in order to expresses cyber image through artificial color combination of sheen colors, Though this techno-color fashion has established the fresh stimulation and the innovative aspect with ultramodern materials and image of futurism, it transmits a hope of estranged people and the natural elements. Hybride means a cross and mixture of animal and plant in Korean and is also called fusion. The phenomenon of hybrid predicts to comes the period of a cross and variation because something completely new comes into the world by contamination, mixture and compromise through meeting something different each other and it has on advantage of developing something existing to one more stage. It is prospected that in the society of 21st century, the borderline of traditional gender will be disappeared, variety and individuality will determine the individual behavior, and the masculine value will be substituted by feminine value. In the society giving priority to feminine value, a fashion stuck closely to women is what must reflect lives of woman under the proposition of woman's beauty, being on original function. So, it is considered that a fashion with added convenience and practicality having the function which is easy to put on, comfortable to act, able to express solves so much, and able to show various appearances according to T.P.O will get into the spotlight.

• 한국표면공학회:학술대회논문집
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• 한국표면공학회 2017년도 춘계학술대회 논문집
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• pp.134.1-134.1
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• 2017

In this study, we propose a new scaffold fabrication method, "direct electro-hydrodynamic jet process," using the initial jet of an electrospinning process and ethanol media as a target. The fabricated threedimensional (3D) fibrous structure was configured with multilayered microsized struts consisting of randomly entangled micro/nanofibrous architecture, similar to that of native extracellular matrixes. The fabrication of the structure was highly dependent on various processing parameters, such as the surface tension of the target media, and the flow rate and weight fraction of the polymer solution. As a tissue regenerative material, the 3D fibrous scaffold was cultured with preosteoblasts to observe the initial cellular activities in comparison with a solid-freeform fabricated 3D scaffold sharing a similar structural geometry. The cell-culture results showed that the newly developed scaffold provided outstanding microcellular environmental conditions to the seeded cells (about 3.5-fold better initial cell attachment and 2.1-fold better cell proliferation).

• The Korean Journal of Physiology and Pharmacology
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• 제26권5호
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• pp.297-305
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• 2022

The presence of artificial light enables humans to be active 24 h a day. Many people across the globe live in a social culture that encourages staying up late to meet the demands of various activities, such as work and school. Sleep deprivation (SD) is a severe health problem in modern society. Meanwhile, as with cardiometabolic disease, there was an obvious tendency that coronary heart disease (CHD) to become a global epidemic chronic disease. Specifically, SD can significantly increase the morbidity and mortality of CHD. However, the underlying mechanisms responsible for the effects of SD on CHD are multilayered and complex. Inflammatory response, lipid metabolism, oxidative stress, and endothelial function all contribute to cardiovascular lesions. In this review, the effects of SD on CHD development are summarized, and SD-related pathogenesis of coronary artery lesions is discussed. In general, early assessment of SD played a vital role in preventing the harmful consequences of CHD.

• Asian-Australasian Journal of Animal Sciences
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• 제15권3호
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• pp.340-345
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• 2002

In this experiment, oocytes were collected from goat ovaries available in slaughterhouse by follicle puncture method. Morphologically culturable type of oocytes which having compact, multilayered cumulus granulosa cell complex and evenly granulated cytoplasm, was separated under a stereozoom microscope. Oocytes were washed thoroughly in maturation medium containing TCM-199, $1{\mu}g/ml$ estradiol-$17{\beta}$, 0.5 ${\mu}g/ml$ FSH, $100{\mu}g/ml$ LH, 3 mg/ml BSA and 10% estrus goat serum. Washed oocytes were cultured into maturation medium on granulosa cell monolayer. Culture plate was then kept into $CO_2$ incubator at $38{\pm}1^{\circ}C$, maximum humidity and 5% $CO_2$ for 18 h. After maturation the oocytes were washed thoroughly with maturation medium containing polyvinyl alcohol (PVA) without serum and BSA and further cultured for 12 h for secretory proteins of oocytes. PVA medium was collected, pooled and concentrated by 5000 cut off centrisart. Secretory proteins were separated on 12.5% SDS-PAGE. A total number of 3.41 oocytes per ovary were obtained and 2.17 culturable oocytes per ovary were cultured into maturation medium. After 18 h of maturation, 4,567 oocytes (1.82 oocytes per ovary) were further cultured into serum and BSA free PVA medium for its secretory proteins. Four secretory proteins of oocytes with approximately molecular weight of 45, 55, 65 and 95 kDa were obtained on SDS-PAGE in silver staining and three proteins with approximately molecular weight of 45, 55 and 65 kDa in Coomassie brilliant blue staining. In conclusion, four secretory proteins with approximately molecular weight of 45, 55, 65 and 95 kDa was obtained from in vitro cultured oocytes of goats.

• 문화기술의 융합
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• 제6권2호
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• pp.515-520
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• 2020

디지털 회로설계 기술에서 사용되는 CMOS는 양자 터널링 현상 등으로 인해 집적도의 한계에 다다르고 있다. 이를 대체할 수 있는 양자점 셀룰러 오토마타(QCA : Quantum-dot Cellular Automata)는 적은 전력 소모와 빠른 스위칭 속도 등으로 많은 장점이 있음으로 CMOS의 많은 디지털 회로들이 QCA 기반으로 제안되었다. 그중에서도 멀티플렉서는 D-플립플롭, 레지스터 등 다양한 회로에 쓰이는 기본 회로로써 많은 연구가 되고 있다. 하지만 기존의 멀티플렉서는 공간 효율성이 좋지 않다는 단점이 있다. 따라서, 본 논문에서는 셀 간 상호작용을 이용하여 새로운 다층구조 멀티플렉서를 제안하고, 이를 이용하여 D-래치를 제안한다. 본 논문에서 제안하는 멀티플렉서와 D-래치는 면적, 셀 개수, 지연시간이 개선되었으며, 이를 이용하여 큰 회로를 설계할 시 연결성과 확장성이 우수하다. 제안된 모든 구조는 QCADesigner를 이용해 시뮬레이션하여 동작을 검증한다.

• 비교문화연구
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• 제44권
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• pp.87-121
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• 2016

식민지 시기 함대훈은 러시아 문학을 자신의 소설 창작에 반복적으로 활용했다. 그 중에서 투르게네프의 소설 "그 전날 밤"은 함대훈의 첫 장편소설 "폭풍전야"에도, 1943년 발표된 "북풍의 정열"에도 반복적으로 차용되고 있다. 함대훈 문학에 차용된 러시아 문학은 지금 이곳과는 다른 문화, 다른 질서에 대한 인물들의 동경을 이끌어냈으며, 인물들의 동경은 1930년대 중반 발표된 "폭풍전야"에서는 민족운동에 뛰어든 신청년(新靑年)의 형상으로 구체화되어 당대 식민지 조선의 상황과 긴장 관계를 만들어냈다. 반면 1943년 발표된 "북풍의 정열"은 "폭풍전야"와 마찬가지로 투르게네프의 "그 전날 밤"을 차용하고 있지만, 이 소설에는 "폭풍전야"와는 변별되는 지정학적 상상력이 구현되어 있다. "북풍의 정열"에서는 '만주'를 둘러싼 당대의 정치적 역사적 맥락은 소거된 반면, '만주'와 과거 지식인 청년들의 열정을 이끌어냈던 '러시아'를 연결시키고 있다. '러시아'에 대한 열정이 만주에 대한 동경으로 대체되는 과정은 함대훈 문학에 나타난 '북국(北國)' 표상의 변화와도 밀접하게 연관된다. 1930년대 후반 함대훈의 소설에서는 '러시아'가 '북국'으로도 표상되고 있었다. 그러나 함대훈 문학에서 '북국'은 점차 '러시아'가 아니라 '만주'를 지칭하는 공간 표상으로 사용되기 시작한다. 북국' 표상을 통해 러시아와 만주를 연결시키는 방식은 '만주'가 시베리아 지방과 멀지 않은 지역임을 부각시키는 언술에서도 암시되어 있듯이 시베리아 지역으로까지 대동아공영권을 확대하고 싶은 제국 일본의 욕망과 긴밀하게 연동되어 있었다. 함대훈은 '북국' 표상을 통해 러시아에 대한 낭만적 동경을, 제국 일본의 지정학적 논리를 정당화하는 담론으로 바꾸어 놓고 있는 것이다. 식민지 시기 함대훈에게 '러시아'는 동경을 불러일으키는 공간이었지만, 그 동경은 '만주'에 대한 함대훈의 인식에서 드러나듯 언제든 세속적 욕망으로 변화될 수 있는 것이었다. 이는 해방 직후 발표된 함대훈 장편소설 "청춘보"에서도 확인된다. 해방 직후는 소련이 냉전질서의 한 축으로 부각되고 러시아어가 한국인의 일상적 담화공간에까지 영향력을 행사했던 시기였다. 함대훈은 "청춘보"를 통해 러시아 문학을 전공했고 소련의 문화를 동경하던 연구자를 주인공으로 등장시킨 후, 그의 시선으로 해방 전후의 북조선 사회를 재현하고 있다. 그 과정에서 이 작품은 러시아의 문화 및 '소련'과 관련된 다층적 표상을 보여주고 있다. 그러한 재현의 양상을 만들어낸 것은 주인공이 지니고 있는 심퍼사이저(sympathizer) 의식, 즉 '동반자 의식'이다. 이러한 주인공의 동반자 의식은 식민지 후반의 조선에서 금지의 대상이었던 '소비에트' 문화의 이국성을 그가 동경하고 있었던 것에 기인한다. 그러나 러시아어가 일상에서 사용될 수 있는 언어이자 영어와 교환될 수 있는 위상을 확보하게 된 해방 직후 '러시아' 및 '소비에트'를 재현하던 주인공의 시선은 변모하게 된다. '붉은 군대'라는 상징 아래 '러시아'와 '소비에트'를 통합적으로 인식하던 시선은 점차 변모하였고, 월남(越南) 이후에는 '러시아'를 '공산주의'라는 도깨비에 의해 점령된 소굴로 간주하기에 이른다. 그 과정에서 '러시아'와 '소비에트'는 분리되어 이해되기 시작했다. 식민지 시기 가장 핵심적인 러시아 문학 연구자이자 번역자로 규정된 함대훈의 해방 이후 행보, 그리고 해방 직후 그가 발표한 소설 "청춘보"의 러시아어 번역/통역가 곽성식의 면모는 해방직후 한국의 러시아 문학 연구가 걸어야 할 침체의 길들을 서사의 형태로 예견하고 있다. 금지된 것에 대한 열망 및 러시아의 이국적 문화에 대한 동경에 의해 수행되던 낭만적 번역, 해방 전후 함대훈 소설의 러시아 표상은 그러한 낭만적 번역의 시대가 종언을 고했음을 보여주고 있는 것이다.