• 한국균학회지
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• 제42권4호
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• pp.333-340
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• 2014

미생물을 이용한 생물학적 방제제의 개발을 위해 연작피해가 없는 순창군 토양으로부터 다양한 미생물 201종을 분리하였고, 이들의 생물학적 활성을 조사하였다. 201종의 분리 세균 중에서 다양한 식물병원성 곰팡이에 대하여 항균활성이 우수한 5종을 선별하였다. 5종의 분리주에 대하여 siderophore를 생산하며 cellulase, protease, amylase와 같은 곰팡이 세포벽 분해효소를 생산하는 능력이 가장 우수한 SCS3 균주를 최종 선별하였다. 최종 선별한 균주 SCS3의 형태학적, 생리학적 및 생화학적 특성을 조사하였고, 16S rRNA 염기서열의 분석에 의해 B. subtilis SCS3으로 명명하였으며, 염기서열 분석에 기반하여 계통수를 작성하였다. 이상의 결과로부터 B. subtilis SCS3은 식물병원성 곰팡이의 방제를 위한 생물학적 방제제로 유용하게 이용될 수 있을 것으로 생각한다.

• Composites Research
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• 제32권6호
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• pp.349-354
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• 2019

복합재료 중심인장선의 구성은 강화재로 사용되는 탄소섬유 및 유리섬유와 기지재로 사용되는 속경화 열경화성 에폭시로 되어있다. 본 연구에서는 가공송전선의 복합소재 중심인장선(ACCC)에 사용되는 에폭시 경화물에 대하여 분석하였다. 다관능성 에폭시 중 4관능성 에폭시(PA 806)을 사용하고 MNAn, HHPA 두가지 경화제 및 경화 촉진제를 사용하여 에폭시 경화물을 만들고 이를 분석하였다. 경화제의 종류 및 경화 촉진제의 함량에 따른 최적조건을 도출하였다.

• 한국미생물·생명공학회지
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• 제28권6호
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• pp.334-340
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• 2000

In order to select multifunctional powerful antagonistic biocontrol agent against red-pepper rotting fungi Phytophthora capsici, we isolated an indigenous antagonistic bacterium which produces antifungal substances and siderophores from a local soil of Kyongju, Korea. The isolated strain was identified as Pseudomonas fluorescens biotype F. The antibiotic produced from P. fluorescens 2112 inhibited hyphae growth and the zoospore germination of Phytophthora capsici. The favorable carbon, nitrogen source and salts for the production of antibiotic from P. fluorescens 2112 were glycerol, beef extract and LiCi at 1.0%, 0.5% and 5 mM, respectively. And antagonistic activity of P. fluorescens 2112 was confirmed against P. capsici in vivo.

• 한국미생물·생명공학회지
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• 제27권5호
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• pp.349-353
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• 1999

Antagonistic Promicromonospora sp. KH-28 isolated from a suppressive soil could produced a chitinase and a antifungal antibiotic for the biocontrol ability. The chitinase and the antibiotic appeared to inhibit plant pathogens of Fusarium oxysporum. Phytophthora capsici, Alternaria kiki, fusarium solani, Stemphylium sp., and Psudomonas fluorescens. the antibiotic produced from the strain was identified as a antifungal substance of 503 dalton having a pyrimidine skeleton with an aliphatic side chain. The Promicromonospora sp. KH-28 was able to suppress effectively F. oxysporum derived-fusarium wilt of red-pepper plant in the pot in vivo test.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2014년도 제46회 동계 정기학술대회 초록집
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• pp.132.1-132.1
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• 2014

Fluorescent-magnetic nanoclusters were synthesized for biomedical applications. The nanoclusters consisted of superparamagnetic core-nanoclusters, highly fluorescent shell of nanocrystals, and lipid A. Magnetic cores were used for both magnetic resonance imaging (MRI) and cell separation. Fluorescent shell was used for optical imaging. The lipid-A-loaded nanoclusters were up-taken by dendritic cells via phagocytosis, which successfully activated dendritic cells. The dendritic cells were migrated to lymph nodes and spleen of mice. The results showed that our novel nanoclusters can play a role as an efficient optical and magnetic imaging, a cell separating and a pathogen mimetic agent at the same time. Additionally, synthesis of wavelength conversion nanowires will be discussed, which may be used as an optical nanoprobe in biological studies.

• 고무기술
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• 제5권2호
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• pp.94-103
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• 2004

The present work describes modification of dual phase filler by electron beam irradiation in presence of multifunctional acrylates like trimethylol propane triacrylate (TMPTA) or silane coupling agent like bis (3-triethoxysilylpropyltetrasulphide) and in-fluence of the modified fillers on the physical properties of styrene-butadiene rubber (SBR) vulcanizates. Modulus at 300 % elongation increases whereas the tensile strength decreases with increase in radiation dose for the dual phase filler loaded styrene-butadiene rubber vulcanizates (SBR). However, modulus and tensile strength significantly increase, which is more, pronounced at higher filler loadings for TMPTA modified dual phase filler loaded SBR. These changes in properties are explained by the equilibrium swelling data and Kraus plot interpreting the polymer-filler interaction. Electron beam modification of the filler results in a reduction of tan ${\delta}$ at $70^{\circ}C$, a parameter for rolling resistance and increase in tan ${\delta}$ at $0^{\circ}C$, a parameter for wet skid resistance of the SBR vulcanizates. Finally, the influence of modified fillers on the properties like abrasion resistance, tear strength and fatigue failure and the improvement in the properties have been explained in terms of polymer-filler interaction.

• Journal of Ginseng Research
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• 제43권1호
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• pp.135-142
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• 2019

Background: Panax ginseng Meyer, known as Korean Red Ginseng (KRG), is one of the important age-old traditional herbs used in boosting libido and improving male fertility. In this study, the effects of Rg3-enriched KRG extract (KGC04P) on heat stress-induced testicular damage in experimental rats was evaluated. Methods: Male rats (Sprague-Dawley) were divided into four groups (n = 10): normal control (NC), heat-stressed control (HC), heat-stressed plus KGC04P-100 mg/kg (HK100), and heat-stressed plus KGC04P-200 mg/kg (HK200) groups. Starting 1 week prior to heat stress, animals were administered orally with KGC04P (100 and 200 mg/kg) mixed with a regular pellet diet and continued for 25 weeks. Heat stress was induced to HC, HK100, and HK200 groups by intermittently exposing the animals to high temperatures ($32{\pm}1^{\circ}C$, 2 h/day). After 6 months, animals were euthanized under general anesthesia with carbon dioxide and evaluated for various parameters in serum and testicular tissue by using Western blotting, biochemical kits, and reverse transcription-polymerase chain reaction. Results: Significant (p < 0.05) alterations in several parameters, such as body/organ weight, sperm kinematics, and lipid metabolism marker levels, in the serum and testis of rats were observed. Further, the expression of testicular antioxidant enzymes, inflammatory cytokines, sex hormonal receptors, and spermatogenesis-related genes were also affected significantly (p < 0.05) in the heat-stressed group. However, KGC04P prevented the heat stress-induced changes in rats significantly (p < 0.05) at both concentrations. Conclusion: KGC04P attenuated heat stress-induced testicular damage by a multifunctional approach and can be developed as an excellent therapeutic agent for hyperthermia-mediated male infertility.

• 농약과학회지
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• 제20권3호
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• pp.189-196
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• 2016

바실러스는 토양 및 반추동물의 소화기관과 같은 다양한 곳에서 분리되고 있으며, 작물의 생육 촉진과 병방제를 위한 미생물 재제로서 널리 사용되고 있다. 바실러스 GH1-13 균주는 전남 완도의 간척지 논에서 분리되었으며, 16S rRNA 유전자와 gyrB 유전자를 이용하여 계통유전학적으로 분석한 결과 Bacillus velezensis인 것으로 동정되었다. GH1-13 균주의 특성을 분석한 결과 생육촉진에 관련된 IAA를 생성할 뿐 아니라 벼 뿌리의 생육을 촉진하는 것을 확인하였다. 또한 벼의 주요 병원균의 생육을 억제할 뿐 아니라 작물의 병원균인 다양한 곰팡이의 생육을 저해하였다. 더불어 식물병원 진균 및 세균의 생육 억제와 밀접하게 관련된 것으로 판단되는 bacillomycin, bacilycin, fengycin, iturin, surfactin을 생성하는 생합성유전자를 보유한 것으로 확인되었다. 본 연구는 GH1-13균주가 작물의 생육촉진과 병 방제를 동시에 해결할 수 있는 강력한 복합기능성 미생물제로의 가능성이 있음을 보여주었다.

• 폴리머
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• 제34권3호
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• pp.226-236
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• 2010

UV조사에 의한 공중합반응을 통해 PDP의 방열패드용 무용제형 아크릴계 점착제를 제조하고 이때 도입되는 공단량체의 곁사슬의 화학적 구조와 가교제의 종류에 따른 점착제의 젖음성을 온도별로 조사하였다. 또한, 동일 조건에서 점착제의 초기 접착력 및 박리강도와 주파수 변화에 따른 점착제의 점탄성 거동 간의 상관관계를 확인하였다. 공단량체의 곁사슬의 길이가 짧을수록 넓은 온도범위에서 우수한 젖음성과 점착 물성을 보였으며 경화제로는 di(ethylene glycol) dimethylacrylate(DEGDMA)를 사용한 계가 우수한 박리 에너지를 보였는데, 이것은 유리전이온도와 점탄성거동의 차이에 기인하는 것으로 판단된다.

• Journal of Microbiology and Biotechnology
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• 제22권11호
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• pp.1568-1574
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• 2012

Microbiological calcium carbonate precipitation (MCCP) has been investigated for its ability to improve the durability of cement mortar. However, very few strains have been applied to crack remediation and strengthening of cementitious materials. In this study, we report the biodeposition of Bacillus subtilis 168 and its ability to enhance the durability of cement material. B. subtilis 168 was applied to the surface of cement specimens. The results showed a new layer of deposited organic-inorganic composites on the surface of the cement paste. In addition, the water permeability of the cement paste treated with B. subtilis 168 was lower than that of non-treated specimens. Furthermore, artificial cracks in the cement paste were completely remediated by the biodeposition of B. subtilis 168. The compressive strength of cement mortar treated with B. subtilis 168 increased by about 19.5% when compared with samples completed with only B4 medium. Taken together, these findings suggest that the biodeposition of B. subtilis 168 could be used as a sealing and coating agent to improve the strength and water resistance of concrete. This is the first paper to report the application of Bacillus subtilis 168 for its ability to improve the durability of cement mortar through calcium carbonate precipitation.