• Korean Journal of Veterinary Service
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• v.37 no.4
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• pp.247-252
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• 2014

The economic impact of swine mycoplasma infection is high. An accurate diagnosis is often difficult and time consuming. We report the development and validation of an effective multiplex polymerase chain reaction (PCR) assay that detects Mycoplasma (M.) hyopneumoniae and M. hyorhinis. The multi detection of M. hyopneumoniae and M. hyorhinis primer set were employed to detect mycoplasma species and typing of the species was performed on the basis of sequence analysis of the PCR product. The target nucleic acid fragments were specifically amplified by M. hyopneumoniae and M. hyorhinis PCR with 16S ribosomal DNA primers. Single and mixed Mycoplasma species DNA templates were used to evaluate the specificity of the multiplex assay. The corresponding specific DNA products were amplified for each pathogen. The multiplex PCR assay provides a novel tool for simultaneous detection and differentiation of M. hyopneumoniae and M. hyorhinis.

• Korean Chemical Engineering Research
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• v.48 no.2
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• pp.244-252
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• 2010

The objective of this study is to assess feasibility of simultaneous analysis for trace multi-components odorous and volatile organic compounds using a Triple-bed adsorbent tube with a thermal desorber and GC-MS. Triple-bed adsorbent tube is 3 bed packed Tenax-TA with small amount of Carbopack B and Carbosieve SIII in order of adsorption strength in a tube. The operating conditions of GC-MS was possibly able to and effectively detect high volatile and low molecular weight compounds at the mass range of 20~350 m/z using a below impurity 1ppm of Helium carrier gas, of which quantitatively analyzed by target ion extracts. According to the experiment, $C_1{\sim}C_5$ of 14 components; sulfur containing compounds(2), ketones(2), alcohols(4) and aldehydes(6) were simultaneously analyzed with recoveries of 99%, and good repeatability and linearity. High volatile and low molecular weight compounds such as methyl alcohol and acetaldehyde can be safely quantified with high recovery at a condition of 50mL/min of flow rate, below 2L of adsorption volume, and 45% of relative humidity. Target ion extract can also simultaneously quantify multicomponents with odorous and volatile organic compounds in an occasion of piled up two peaks.

• The Korean Journal of Pesticide Science
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• v.9 no.4
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• pp.311-315
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• 2005

The simultaneous or consecutive product analysis is needed for quality control because the various items are produced in pesticide manufacturer. This study was conducted to establish a multi-pesticide analysis making possible to analyze several active ingredients with one injection of mixed active ingredients under same instrument-condition in the cause of quality control with accuracy and speed. The test was conducted with 3 pesticides, iprobenfos 17%GR, isoprothiolane 12%GR, tebufenozide 20%SC and performed by GLC and HPLC. With the GLC method, 2 active ingredients of iprobenfos and isoprothiolane were analyzed but tebufenozide was not detective simultaneously. With the HPLC method, all of the active ingredients in those three pesticides were simultaneously analyzed in this study.

• Journal of Advanced Marine Engineering and Technology
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• v.28 no.6
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• pp.981-992
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• 2004

Freeze drying under vacuum condition is a complex process that involves simultaneous heat and mass transfer, sublimation of ice, and motion of sublimation front. Proper treatment of the motion of sublimation interface is crucial for an accurate prediction of the freeze drying process. Based on the enthalpy formulation that has been successfully used in liquid/solid phase change problems. a fixed grid method. streamlined for the freeze drying analysis. was developed in this study. The accuracy of the fixed grid method was checked by solving a one-dimensional tray freeze drying and a two-dimensional vial freeze drying problem and then comparing the results with those by the moving grid method. Finally. the freeze drying characteristics of two-dimensional slab and axis-symmetric cylinder was investigated using the fixed grid method.

• Transactions of the Korean Society of Automotive Engineers
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• v.12 no.3
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• pp.51-58
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• 2004

Optimization of engine desist and operation parameters using a genetic algorithm was demonstrated for direct injection diesel engine combustion. A micro genetic algorithm and a modified KIVA-3V code were used for the analysis and optimization of the engine combustion. At each generation of the optimization step the micro genetic algorithm generated five groups of parameter sets, and the five cases of KIVA-3V analysis were to be performed either in series or in parallel. The micro genetic algorithm code was also parallelized by using MPI programming, and a multi-CPU parallel supercomputer was used to speed up the optimization process by four times. An example case for a fixed engine speed was performed with six parameters of intake swirl ratio, compression ratio, fuel injection included angle, injector hole number, SOI, and injection duration. A simultaneous optimization technique for the whole range of engine speeds would be suggested for further studies.

• Journal of the Korean Institute of Telematics and Electronics
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• v.15 no.4
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• pp.14-19
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• 1978

Here a method is shown to extract features from two multi-variable classes by using the coordinate systems transformed by one-class and mixture normalization algorithms. Some properties and implemented results of this technique are described. Also, comparision of these features with factor analysis results is performed. This method is thought to be more powerful one, in feature extraction sense, than factor analysis.

• International Journal of Fuzzy Logic and Intelligent Systems
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• v.5 no.3
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• pp.268-273
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• 2005

We propose the specialized product-line development methodology for developing the embedded system of an MSDFS (Multi Sensor Data Fusion System : called MSDFS). The product-line methodology provides a simultaneous design between software and hardware, high level reusability. However this is insufficient in requirement analysis stage due to be focused on software architecture, detailed design and code. Thus we apply the business model based on IDEF0 technique to traditional methodology. In this paper, we describe the processes of developing Core-Asset, which are requirement analysis, feature modeling, validation. The proposed model gives the efficient result for eliciting features, and ensures the high level reusability of modules performing on embedded system.

• Korean Journal of Environmental Agriculture
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• v.41 no.4
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• pp.288-309
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• 2022

BACKGROUND: The objective of this study was to develop a comprehensive and simple method for the simultaneous determination of 59 veterinary drug residues in livestock products for safety management. METHODS AND RESULTS: For sample preparation, we used a modified liquid extraction method, according to which the sample was extracted with 80% acetonitrile followed by incubation at -20℃ for 30 min. After centrifugation, an aliquot of the extract was evaporated to dryness at 40℃ and analyzed using liquid chromatography combined with tandem mass spectrometry. The method was validated at three concentration levels for beef, pork, chicken, egg, and milk in accordance with the Codex Alimentarius Commission/Guidelines 71-2009. Quantitative analysis was performed using a matrix-matched calibration. As a results, at least 52 (77.6%) out of 66 compounds showed the proper method validation results in terms of both recovery of the target compound and coefficient of variation required by Codex guidelines in livestock products. The limit of quantitation of the method ranged from 0.2 to 1119.6 ng g-1 for all matrices. CONCLUSION(S): This method was accurate, effective, and comprehensive for 59 veterinary drugs determination in livestock products, and can be used to investigate veterinary drugs from different chemical families for safety management in livestock products.

• The Journal of the Korea Contents Association
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• v.7 no.2
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• pp.84-91
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• 2007

Recently, the researches based on vision about user attention and action awareness are being pushed actively for human computer interaction. Among them, various applications of tabletop display system are developed more in accordance with touch sensing technique, co-located and collaborative work. Formerly, although supported only one user, support multi-user at present. Therefore, collaborative work and interaction of four elements (human, computer, displayed objects, physical objects) that is ultimate goal of tabletop display are realizable. Generally, tabletop display system designs according to four key aspects. 1)multi-touch interaction using bare hands. 2)implementation of collaborative work, simultaneous user interaction. 3)direct touch interaction. 4)use of physical objects as an interaction tool. In this paper, we describe a critical analysis of the state-of-the-art in advanced multi-touch sensing techniques for tabletop display system according to the four methods: vision based method, non-vision based method, top-down projection system and rear projection system. And we also discuss some problems and practical applications in the research field.

• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.252-252
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• 2022

Genotype-by-environment interaction (GEI) refers to the comparative response of genotypes to different environments conditions. Thus, understanding GEI is a fundamental component for selecting superior genotypes for breeding programs. The significance of utilizing early maturing cultivars not only provides flexibility in planting dates, but also serves as an effective strategy to reduce methane emission from the paddy fields. In this study, we conducted multi-environment trials (METs) to evaluate yield-related traits such as culm length, panicle length, panicle number, spikelet per plant, and thousand grain weight. A total of eighty-one Korean commercial rice cultivars categorized as early maturing cultivars, were cultivated in three regions, two planting seasons for two years. The genotype main effect plus genotype-by-environment interaction (GGE) biplot analysis of yield-related traits and grain yield explained 70.02-91.24% of genotype plus GEI variation, and exhibited various patterns of mega-environment delineation, discriminating ability, representativeness, and genotype rankings across the planting seasons and environments. Moreover, simultaneous selection using weighted average of absolute scores from the singular value decomposition (WAASB) and multi-trait stability index (MTSI) revealed six highly recommended genotypes with high stability and crop productivity. The winning genotypes under specific environment can be utilized as useful genetic materials to develop regional specialty cultivars, and recommended genotypes can be used as elite climate-resilient parents to improve yield-potential and reduce methane emission as part to accomplish carbon-neutrality.