Korean Journal of Environmental Agriculture (한국환경농학회지)

The Korean Society of Environmental Agriculture (한국환경농학회)
권호 표지
DOI QR코드

DOI QR Code

Multi-class, Multi-residue Analysis of 59 Veterinary Drugs in Livestock Products for Screening and Quantification Using Liquid Chromatography-tandem Mass Spectrometry

  • Yu Ra Kim (Pesticide and Veterinary Drug Residues Division, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety) ;
  • Sun Young Park (Pesticide and Veterinary Drug Residues Division, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety) ;
  • Tae Ho Lee (Pesticide and Veterinary Drug Residues Division, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety) ;
  • Ji Young Kim (Pesticide and Veterinary Drug Residues Division, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety) ;
  • Jang-Duck Choi (Pesticide and Veterinary Drug Residues Division, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety) ;
  • Guiim Moon (Pesticide and Veterinary Drug Residues Division, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety)
  • Received : 2022.10.25
  • Accepted : 2022.12.12
  • Published : 2022.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

BACKGROUND: The objective of this study was to develop a comprehensive and simple method for the simultaneous determination of 59 veterinary drug residues in livestock products for safety management. METHODS AND RESULTS: For sample preparation, we used a modified liquid extraction method, according to which the sample was extracted with 80% acetonitrile followed by incubation at -20℃ for 30 min. After centrifugation, an aliquot of the extract was evaporated to dryness at 40℃ and analyzed using liquid chromatography combined with tandem mass spectrometry. The method was validated at three concentration levels for beef, pork, chicken, egg, and milk in accordance with the Codex Alimentarius Commission/Guidelines 71-2009. Quantitative analysis was performed using a matrix-matched calibration. As a results, at least 52 (77.6%) out of 66 compounds showed the proper method validation results in terms of both recovery of the target compound and coefficient of variation required by Codex guidelines in livestock products. The limit of quantitation of the method ranged from 0.2 to 1119.6 ng g-1 for all matrices. CONCLUSION(S): This method was accurate, effective, and comprehensive for 59 veterinary drugs determination in livestock products, and can be used to investigate veterinary drugs from different chemical families for safety management in livestock products.

Keywords

Acknowledgement

This work was supported by the Ministry of Food and Drug safety of Republic of Korea (grant no. 21161MFDS603) in 2021.

References

  1. Bretschneider G, Elizalde JC, Perez FA (2008) The effect of feeding antibiotic growth promoters on the performance of beef cattle consuming forage-based diets: A review. Livestock Science, 114(2-3), 135-149. https://doi.org/10.1016/j.livsci.2007.12.017.
  2. Kelly L, Smith DL, Snary EL, Johnson JA, Harris AD, Wooldridge M, Morris Jr JG (2004) Animal growth promoters: To ban or not to ban? A risk assessment approach. International Journal of Antimicrobial Agents, 24(3), 205-212. https://doi.org/10.1016/j.ijantimicag.2004.04.007.
  3. Ternak G (2005) Antibiotic may cat as growth/obesity promoters in humans as an inadvertent result of antibiotic pollution?. Medical Hypotheses, 6(1), 14-16. https://doi.org/10.1016/j.mehy.2004.08.003.
  4. Wegener HC (2003) Antibiotics in animal feed and their role in resistance development. Current Opinion in Microbiology, 6(5), 439-445. https://doi.org/10.1016/j.mib.2003.09.009.
  5. Lopes RP, Reyes RC, Romero-Gonzalez R, Frenich AG, Vidal JLM (2012) Development and validation of a multiclass method for the determination of veterinary drug residues in chicken by ultrahigh performance liquid chromatography-tandem mass spectrometry. Talanta, 89, 201-208. https://doi.org/10.1016/j.talanta.2011.11.082.
  6. Albright JL, Tuckey SL, Woods GT (1961) Antibiotics in milk-A Review. Journal of Dairy Science, 44(5), 779-807. https://doi.org/10.3168/jds.S0022-0302(61)89819-6.
  7. Herz U, Lacy P, Renz H, Erb K (2000) The influence of infections on the development and severity of allergic disorders. Current Opinion in Immunology, 12(6), 632-640. https://doi.org/10.1016/S0952-7915(00)00155-2.
  8. Kemper N (2008) Veterinary antibiotics in the aquatic and terrestrial environment. Ecological Indicators, 8(1), 1-13. https://doi.org/10.1016/j.ecolind.2007.06.002.
  9. Martinez JL (2009) Environmental pollution by antibiotics and by antibiotic resistance determinants. Environmental Pollution, 157(11), 2893-2902. https://doi.org/10.1016/j.envpol.2009.05.051.
  10. Gustafson RH (1991) Use of antibiotics in livestock and human health concerns. Journal of Dairy Science, 74(4), 1428-1432. https://doi.org/10.3168/jds.S0022-0302(91)78299-4.
  11. Sorum H, L'Abee-Lund TM (2002) Antibiotic resistance in food-related bacterial-a result of interfering with the global web of bacterial genetics. International Journal of Food Microbiology, 78(1-2), 43-56. https://doi.org/10.1016/S0168-1605(02)00241-6.
  12. Wallmann J (2006) Monitoring of antimicrobial resistance in pathogenic bacteria from livestock animals. International Journal of Medical Microbiology, 296, 81-86. https://doi.org/10.1016/j.ijmm.2006.01.064.
  13. Ellis RL (2008) Development of veterinary drug residue controls by the Codex Alimentarius Commission: a review. Food Additives & Contaminants: Part A, 25(12), 1432-1438. https://doi.org/10.1080/02652030802267405.
  14. Zhao L, Lucas D, Long D, Richter B, Stevens J (2018) Multi-class multi-residue analysis of veterinary drugs in meat using enhanced matrix removal lipid cleanup and liquid chromatography-tandem mass spectrometry. Journal of Chromatography A, 1549, 14-24. https://doi.org/10.1016/j.chroma.2018.03.033.
  15. Gressler V, Franzen AR, de Lima GJ, Tavernari FC, Dalla Costa OA, Feddern V (2016) Development of a readily applied method to quantify ractopamine residue in meat and bone meal by QuEChERS-LC-MS/MS. Journal of Chromatography B, 1015-1016, 192-200. https://doi.org/10.1016/j.jchromb.2016.01.063.
  16. Zhang Y, Liu X, Li X, Zhang J, Cao Y, Su M, Shi Z, Sun ZH (2016) Rapid screening and quantification of multi-class multi-residue veterinary drugs in royal jelly by ultra performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry. Food Control, 60, 667-676. https://doi.org/10.1016/j.foodcont.2015.09.010.
  17. Pyo MJ, Hah DY, Choi YJ, Jeong KO, Han CH, Park YH, Kim WG, Jung JG, Kim MK et al. (2015) QuEChERS-based determination of tissue residues and acute toxicity of pyraclofos in rat. Korean Journal of Veterinary Service, 38(3), 173-180. https://doi.org/10.7853/kjvs.2015.38.3.173.
  18. Kang J, Fan CL, Chang QY, Bu MN, Zhao ZY, Wang W, Pang GF (2014) Simultaneous determination of multi-class veterinary drug residues in different muscle tissues by modification QuEChERS combined with HPLC-MS/MS. Analytical Methods, 6, 6285-6293. https://doi.org/10.1039/C4AY00589A.
  19. Zhu WX, Yang JZ, Wang ZX, Wang CJ, Liu YF, Zhang L (2016) Rapid determination of 88 veterinary drug residues in milk using automated TurborFlow online clean-up mode coupled to liquid chromatography-tandem mass spectrometry. Talanta, 148, 401-411. https://doi.org/10.1016/j.talanta.2015.10.037.
  20. Boscher A, Guignar, C, Pellet T, Hoffmann L, Bohn T (2010) Development of a multi-class method for the quantification of veterinary drug reisudes in feedingstuffs by liquid chromatography-tandem mass spectrometry. Journal of Chromatography A, 1217 (41), 6394-6404. https://doi.org/10.1016/j.chroma.2010.08.024.
  21. De Ruyck H, Daeseleire E, Ridder H, van Renterghem R (2002) Development and validation of a liquid chromatographic-electrospray tandem mass spectrometric multiresidue method for anthelmintics in milk. Journal of Chromatography A, 976(1-2), 181-194. https://doi.org/10.1016/S0021-9673(02)00936-6.
  22. Lu Y, Shen Q, Dai Z, Zhang H, Wang H (2011) Development of an on-line matrix solid-phase dispersion/fast liquid chromatography/tandem mass spectrometry system for the rapid and simultaneous determination of 13 sulfonamides in gas carp tissues. Journal of Chromatography A, 1218(7), 929-937. https://doi.org/10.1016/j.chroma.2010.12.064.
  23. Maldaner L, Santana CC, Jardim IC (2008) HPLC determination of pesticides in soybeans using matrix solid phase dispersion. Journal of Liquid Chromatography & Related Technologies, 31(7), 972-983. https://doi.org/10.1080/10826070801924675.
  24. Martinez Vidal JL, Frenich AG, Aguilera-Luiz MM, Romero-Gonzalez R (2010) Development of fast screening methods for the analysis of veterinary drug residues in milk by liquid chromatography-triple quadrupole mass spectrometry. Analytical and Bioanalytical Chemistry, 397(7), 2777-2790. https://doi.org/10.1007/s00 216-009-3425-1.
  25. Yamada R, Kozono M, Ohmori T, Morimatsu F, Kitayama M (2006) Simultaneous determination of residual veterinary drugs in bovine, porcine, and chicken muscle using liquid chromatography coupled with electrospray ionization tandem mass spectrometry. Bioscience, Biotechnology, and Biochemistry, 70(1), 54-65. https://doi.org/10.1271/bbb.70.54.
  26. Kaufmann A, Butcher P, Maden K, Widmer M (2008) Quantitative multiresidue method for about 100 veterinary drugs in different meat matrices by sub 2-µm particulate high-performance liquid chromatography coupled to time of flight mass spectrometry. Journal of Chromatography A, 1194(1), 66-79. https://doi.org/10.1016/j.chroma.2008.03.089.
  27. Codex Committee on Residues of Veterinary Drugs in Foods (2012) Guidelines for the design and implementation of national regulatory food safety assurance programme associated with the use of veterinary drugs in food producing animals (CAC/GL 71-2009). pp. 1-30, Codex Alimentarius Commission, Rome, Italy.
  28. Kim EJ, Park HJ, Park SH, Choi JD, Yun HJ, Kim JH (2021) Simultaneous determination of multi-class veterinary drugs in fishery products with liquid chromatography-tandem mass spectrometry. Applied Biological Chemistry, 64, 40. https://doi.org/10.1186/s13765-021-00611-8.
  29. Dasenaki ME, Thomaidis NS (2015) Multi-residue determination of 115 veterinary drugs and pharmaceutical residues in milk powder, butter, fish tissue and eggs using liquid chromatography-tandem mass spectrometry. Analytical Chimica Acta, 880, 103-121. https://doi.org/10.1016/j.aca.2015.04.013.
  30. Villar-Pulido M, Gilbert-Lopez B, Garcia-Reyes JF, Martos NR, Molina-Diaz A (2011) Multiclass detection and quantitation of antibiotics and veterinary drugs in shrimps by fast liquid chromatography time of flight mass spectrometry. Talanta, 85(3), 1419-1427. https://doi.org/10.1016/j.talanta.2011.06.036.
  31. Deng XJ, Yang HQ, Li JZ, Song Y, Guo DH, Luo Y, Du XN, Bo T (2011) Multiclass residues screening of 105 veterinary drugs in meat, milk, and egg using ultra high performance liquid chromatography tandem quadrupole time-of-flight mass spectrometry. Journal of Liquid Chromatography & Related Technologies, (34)19, 2286-2303. https://doi.org/10.1080/10826076.2011.587224.
  32. Dasenaki ME, Bletsou AA, Koulis GA, Thomaidis NS (2015) Qualitative multiresidue screening method for 143 veterinary drugs and pharmaceuticals in milk and fish tissue using liquid chromatography quadrupole-time-of-flight mass spectrometry. Journal of Agricultural and Food Chemistry, 63(18), 4493-4508. https://doi.org/10.1021/acs.jafc.5b00962.
  33. Biselli S, Schwalb U, Meyer A, Hartig L (2012) A multi-class, multi-analyte method for routine analysis of 84 veterinary drugs in chicken muscle using simple extraction and LC-MS/MS. Food Additives & Contaminants: Part A, 30(6), 921-939. https://doi.org/10.1080/19440049.2013.777976.
  34. Stubbings G, Bigwood T (2009) The development and validation of a multiclass liquid chromatography tandem mass spectrometry (LC-MS/MS) procedure for the determination of veterinary drug residues in animal tissue using QuEChERS (QUick, Easy, CHeap, Effective, Rugged and Safe) approach. Analytica Chimica Acta, 637(1-2), 68-78. https://doi.org/10.1016/j.aca.2009.01.029.
  35. Zhao S, Jian H, Li X, Mi T, Li C, Shen J (2007) Simultaneous determination of trace levels of 10 quinolones in swine, chicken, and shrimp muscle tissues using HPLC with programmable fluorescence detection. Journal of Agricultural and Food Chemistry, 55(10), 3829-3834. https://doi.org/10.1021/jf0635309.
  36. Gajda A, Posyniak A, Zmudzki J, Gbylik M, Bladek T (2012) Determination of (fluoro)quinolones in eggs by liquid chromatography with fluorescence detection and confirmation by liquid chromatography-tandem mass spectrometry. Food Chemistry, 135(2), 430-439. https://doi.org/10.1016/j.foodchem.2012.04.106.
  37. Kumar P, Companyo R (2011) Development and validation of an LC-UV method for the determination of sulfonamides in animal feeds. Drug Testing and Analysis, 4(5), 368-375. https://doi.org/10.1002/dta.296.
  38. Hermo MP, Barron D, Barbosa J (2006) Development of analytical methods for multiresidue determination of quinolones in pig muscle samples by liquid chromatography with ultraviolet detection, liquid chromatography-mass spectrometry and liquid chromatography-tandem mass spectrometry. Journal of Chromatography A, 1104(1-2), 132-139. https://doi.org/10.1016/j.chroma.2005.11.080.
  39. Kim YR, Kang HS (2021) Multi-residue determination of twenty aminoglycoside antibiotics in various food matrices by dispersive solid phase extraction and liquid chromatography-tandem mass spectrometry. Food Control, 130, 108374. https://doi.org/10.1016/j.foodcont.2021.108374.
  40. Hou X, Lei S, Qiu S, Guo L, Yi S, Liu W (2014) A multi-residue method for the determination of pesticides in tea using multi-walled carbon nanotubes as a dispersive solid phase extraction absorbent. Food Chemistry, 153, 121-129. https://doi.org/10.1016/j.foodchem.2013.12.031.
  41. Freitas A, Barbosa J, Ramos F (2014) Multi-residue and multi-class method for the determination of antibiotics in bovine muscle by ultra-high-performance liquid chromatography tandem mass spectrometry. Meat Science, 98(1), 58-64. https://doi.org/10.1016/j.meatsci.2014.04.003.
  42. Kim J, Suh JH, Cho HD, Kang W, Choi YS, Han SB (2016) Analytical method for fast screening and confirmation of multi-class veterinary drug residues in fish and shrimp by LC-M/SMS. Food Additives & Contaminants: Part A, 33(3), 420-432. https://doi.org/10.1080/19440049.2016.1139752.
  43. Valese AC, Molognoni L, de Souza NC, de Sa Ploencio LA, Costa ACO, Barreto F, Daguer H (2017) Development, validation and different approaches for the measurement uncertainty of a multi-class veterinary drugs residues LC-MS method for feeds. Journal of Chromatography B, 1053, 48-59. https://doi.org/10.1016/j.jchromb.2017.03.026.
  44. Park H, Kim J, Kang HS, Cho BH, Oh JH (2020) Multiresidue analysis of 18 dye residues in animal products by liquid chromatography-tandem mass spectrometry. Journal of Food Hygiene and Safety, 35(2), 109-117. https://doi.org/10.13103/JFHS.2020.35.2.109.