• 한국동물학회지
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• 제19권1호
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• pp.25-32
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• 1976

양서류 발생에 따른 위점막 점액세포의 조직학적 및 조직화학적인 변화를 밝히고저 개구리(Rana nigromaculata)변태 각 단계의 위체부 조직을 10% formalin 완충액에 고정($4^{\circ}C$), paraffin에 포매, 4 $\mu$m 두께로 절편한후 periodic acid-Schiff(PAS) 및 alcian blue(AB) pH 2.5, pH 1.0에 반응시켜 다음과 같은 결과를 얻었다. 1. 위 표면점액세포내 점액질은 변태과정에 따라 특이한 변화없이 PAS에 강한 반응을 AB pH 2.5 및 pH 1.0에서는 약한 alcianophilia를 보였으며, 변태 XXIV와 XXV에서 세포내 중성 점액질의 함량이 현저히 증가하였다. 2. 위소와점액세포는 변태 XXI이후에서 볼 수 있었는데 PAS에 강한 반응을, AB pH 2.5 및 pH 1.0에서 약한 alcianophilia를 보였으나, AB pH 1.0에서는 AB pH 2.5에서 보다 강하였고, 변태 XXIV와 XXV에서 세포내 중성 점액질의 함량이 현저히 증가하였다. 3. 분비능을 보이는 점액질세포는 변태 XXIV이후인데 변태 XXIV에서는 PAS에 강양성을, AB pH 1.0에서는 약한 alcianophilia를 보였고, 변태 XXV 이후에는 PAS에 약한 반응을, AB pH 2.5에는 약하고 AB pH 1.0에는 중등도의 alcianiphilia를 보였는데 이들 점액질은 전 세포질에 비교적 고르게 분포하였다.

• 대한한방내과학회지
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• 제25권3호
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• pp.497-507
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• 2004

This study was carried out to investigate the effects of Ikwitang extract on indomethacin-induced gastric mucosal lesions in mice. Experimental groups were classified into a non-treated group(NM group), a non-administered group(CON group), the Misoprostol-administered group(MI group) and the Ikwitang-extract-administered group(IW group). This study examined the morphological change, distribution of mast cells, mucous surface cells, acid mucose secreted cells, and apoptic cells, BrdU, COX-1, Hsp70, $NF-{\kappa}B\;p50$, PKC, COX-2 and IL-12B of gastric mucosa. Results : The results of this study were as follows: 1. In the Misoprostol-administered group and the Ikwitang-extract-administered group, the hemorrhagic erosion of gastric mucous and infiltrated mast cells decreased. 2. Mucous surface cells and acid mucose secreted cells abserved in the Misoprostol-administered group and the Ikwitang-extract administered group. 3. The distribution of apoptic cells, Hsp70, $NF-{\kappa}B\;p50$, PKC, COX-2, and IL-12B increased in the Control group, but decreased in the Misoprostol-administered group and Ikwitang-extract-administered group. 4. Cells proliferation of gastric mucosa and the COX-l positive cells decreased in the Control group, but increased in the Misoprostol-administered group and the Ikwitang-extract -administered group. The above results suggest the lkwitang extract had beneficial effects on indomethacin induced gastric mucosal lesions.

• Applied Microscopy
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• 제34권2호
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• pp.139-143
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• 2004

돌돔 아가미의 미세구조를 광학현미경과 투과전자현미경을 이용하여 관찰하였다. 아가미는 새궁 앞, 뒤 2열로 된 빗모양의 많은수의 새엽과 그리고 각 새엽은 전후 두 개의 새판(gill lamellae)이 근접하여 2열로 배열되어 있다. 상피세포층은 단층으로 큰 핵을 가진 편평상피로 구성되고 표면에는 미세융기가 잘 발달되어 있다. 새판의 기둥 구조는 세로 방향의 미세소관들과 측면 membrane interdigitation을 가지며, 염세포는 수많은 미토콘드리아와 tubular system이 잘 발달되어 있다. 미성숙된 점액세포는 조면세포체와 골지체가 잘 발달되었으며 일부 발달된 점액 과립을 가지고 있다. 성숙한 점액세포는 AB-PAS에 푸른 색을 띄었으며 구형이고 다양한 전자밀도를 가진 거의 같은 크기의 점액과립을 가지고 있다.

• ALGAE
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• 제29권3호
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• pp.197-201
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• 2014

This is the first recorded observation of Akashiwo sanguinea excreting mucilaginous substances from pores on the cell surface. Observations were from samples collected in the Caloosahatchee Estuary, Florida, USA during a bloom event, with densities of 672 cells $mL^{-1}$ of A. sanguinea, including 51 cells surrounded by mucous. Scanning electron microscopy observations revealed that the mucous was secreted from thecal pores on the cell surface. The potential significance of mucous production is discussed.

• 대한한방내과학회지
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• 제29권4호
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• pp.939-949
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• 2008

Objective : This study was carried out to investigate the effects of Jisildochehwan extract on indomethacin-induced gastric mucosal lesions of mice. as compared with misoprostol. Methods : Experimental mice were classified into four groups. No gastro-inflammation elicited mice were the normal group(NOR). Gastro-inflammation elicited mice were the control group (CON). Misoprostol-administered mice after gastro-inflammation elicitation were the misoprostol-administered group (MA). Jisildochehwan-administered mice after gastro-inflammation elicitation were the Jisildochehwan-administered group(JA). In this study, we examined superoxide dismutase(SOD) ability, anti-inflammatory ability arrangement of mucous-secreted cells. distribution of mucosal neck cells, mucosal surface cells. neutral mucous-secreted cells. PAS reaction. COX-1, $TNF-{\alpha}$, $NF-{\kappa}B$ p65 and iNOS. Results : The SOD ability of the Jisildochehwan group increased dose-dependently. The hemorrhagic erosion and the damage of arrangement of mucous-secreted cells increased in the CON group. but decreased in the MA and JA groups. The COX-1 positive were decreased in the CON group, but increased in the MA and JA groups. The distribution of mucosal neck cells and mucosal surface cells. PAS positive reaction of surface mucous cells were decreased in CON group, but increased in MA and JA group. $TNF-{\alpha}$, $NF-{\kappa}B$ p65 and iNOS increased in the CON group, but decreased in the MA and JA groups. In all the results, the effects were better in the JA group than in the MA group. Conclusion : Jisildochehwan extract was more effective than Misoprostol. Jisildochehwan has excellent protective effects on indomethacin-induced gastric mucosal lesions.

• 대한수의학회지
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• 제32권4호
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• pp.463-479
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• 1992

The morphological findings of the parotid, mandibualr and sublingual salivary glands of the Korean native goat have been investigated by the histological and histochemical observation using the light microscope. Tissues were fixed with 10% neutral buffered formalin and Bouin's solution, and embedded in paraffin. The tissue sections were stained with hematoxylin and eosin, Heidenhain's azocarmine-aniline blue, alcian blue, toluidine blue, periodic acid Schiff, aldehyde fuchsin, alcian blue-periodic acid Schiff and aldehyde fuchsin-alcian blue. Some sections were stained with the alcian blue after each teatment of diastase digestion, methylation, methylation-saponification, and neuraminidase digestion. The results were as follows ; 1. The major salivary glands were compound. tubuloacinar glands, and the parenchyma was composed of acini, intercalated ducts, striated ducts and excretory ducts. 2. The acini were composed of serous cells in the parotid gland, and mucous cells, serous cells and seous demilunes in the mandibular gland. The acini of the sublingual glands were composed of mucous cells and serous demilunes. 3. In histochemistry, the serous cells of the parotid gland contained neutral mucin and enzyme-liable silaic acid. 4. The serous cells and demilunes of the mandibular gland contained neutral mucin and enzyme-liable sialic acid, and the mueous cells contained sulfated mucin, enzyme liable sialic acid and neutral mucin 5. In the sublingual gland, the mucous cells contained sulfated mucin, enzyme-resistant sialic acid and neutral mucin, and the serous demilunes contained neutral mucin and enzyme-resistant sialic acid.

• Applied Microscopy
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• 제46권1호
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• pp.51-57
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• 2016

The histology and anatomy of the olfactory organ in Luciogobius guttatus was investigated using a light microscopy and scanning electron microscopy. The paired olfactory organs in the dorsal part of the snout are situated in between the upper lip and the eyes. They consist of two nostrils, one anterior and the other posterior openings, and a single olfactory cavity. The anterior nostril, an incurrent opening, forms a short tubular structure from the skin. The posterior nostril, an excurrent opening, forms a circular structure opened to the exterior. The distributional pattern of the sensory epithelium is a continuous type. The sensory epithelium with numerous-motile cilia is made up of receptor cells, supporting cells, basal cells, and mucous cells. In contrast, the non-sensory epithelium is comprised of stratified epithelial cells and two types of mucous cells, acidic and neutral cells. The cilia number of the receptor cell is in range of 3 to 4 units. Such results in L. guttatus may reflect its ecological habit and microhabitat in the tidal zone with a periodic tide.

• 한국어류학회지
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• 제11권2호
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• pp.134-138
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• 1999

피부흡충인 Entobdella hippoglossi가 숙주인 Atlantic halibut의 피부에 기생할 때 기생 부위별 밀도와 숙주의 피부 점액세포 밀도를 조사하여 E. hippoglossi의 기생이 피부점액 세포의 밀도와 관계가 있는가를 조사하였다. 숙주인 Atlantic halibut 복측과 배측에 기생하는 E. hippoglossi의 밀도는 비슷하였으나 기생충의 크기는 복측이 배측보다 더 컸으며, 배측내에서는 배측의 머리부분에 기생하는 E. hippoglossi의 밀도가 배측의 중앙부나 꼬리부분에서보다 높았다. 숙주의 점액세포 크기, 밀도 그리고 표피 점액층의 두께는 대체로 기생충의 밀도와 비례하였고, 숙주내에서 기생충의 선택적 기생은 점액세포의 밀도와 관계가 있었다.

• Animal cells and systems
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• 제11권2호
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• pp.183-189
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• 2007

To investigate the properties of mucosubstances of the epidermis in various teleostean species, conventional histochemical stainings were used on the skin in five species of order Perciformes, i. e., yellowtail, Seriota quinqueradiat, striped beakperch, Oplegnathus fasciatus, brown spotted grouper, Epinephelus chlorostigma, sea chub, Ditrema temmincki and multicolorfin rainbowfish, Halichoeres poecilopterus. The following methods were used: periodic acid Schiff (PAS), alcian blue (AB) pH at 2.5, AB pH at 1.0, AB pH at 2.5-PAS, AB pH at 1.0-PAS, aldehyde fuchsin (AF) pH at 1.7-AB pH at 2.5 and high iron diamine (HID)-AB pH at 2.5. The epidermis of all five species consisted of three layers: superficial, middle, and basal layer. The superficial layer was comprised of rather flattened cells. In particular, the outermost layer of striped beakperch and middle layer of sea chub consisted of mucus-secreting cells. Mucous cells, the unicellular glands, were found in epidermis but varied in number in different body regions and species. Although there was a slight difference in the amount in various species and body regions, the secretory contents of the mucous cells in the five teleostean species contained acidic mucopolysaccharides. In yellowtail, striped beakperch, and multicolorfin rainbowfish, the property of mucosubstances was identified as sialomucin, while it was sulphomucin in brown spotted grouper and sea chub.

• Applied Microscopy
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• 제25권3호
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• pp.40-50
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• 1995

The results of observation on the salivary gland and salivary secretory duct of Korean slug, Incilaria fruhstorferi, in histochemical method are as follows. It is observed that there are six kinds of gland cells(Type-A, B, C, E and F) making up the salivary gland of Korean slug. Of those, type-A gland cell is observed as an acid mucous cell, and type-B, C, D, F gland cells as neutral mucous cells. But in type-E gland cell, while the membrane surrounding granules exhibit alcianophilia, granules show no reaction. The salivary secretory duct composing the salivary gland of Korean slug is composed of supporting epithelial cell and four kinds of gland cells(type-A, E, F and G), of which type-A, E, F gland cells compose both the acinus of salivary gland and endothelial tissue of salivary secretory duct, and are secreted into lumen through salivary secretory duct. But, type-G gland cell is observed only in the endothelium of salivary secretory duct and mucous granules are observed as neutral mucopolysaccharide.