• 제목/요약/키워드: mtDNa

검색결과 548건 처리시간 0.133초

초음파를 이용한 출토 인골 DNA 추출법 연구 (Applied Research of Ultra Sonication for Ancient DNA Preparation of Excavated Human Skeletal Remains)

  • 김윤지;지상현;홍종욱
    • 보존과학연구
    • /
    • 통권29호
    • /
    • pp.137-148
    • /
    • 2008
  • 고고 유적지에서 출토된 뼈에서 추출한 DNA는 부식산과 풀빅산 등의 토양성분과 콜라겐 등 다양한 오염물질이 포함되어 있어 DNA의 추출 및 분석이 매우 어렵다. 본 연구에서는 phenol 추출법, silica 추출법 등 두 가지 대표적인 고대 DNA 추출법의 효율을 DNA 증폭 결과에 의하여 비교하였다. 또한 울트라급의 초음파를 시료 용해에 적용한 후 silica 추출법으로 DNA를 분리한 방법이 기존의 phenol 및 silica 추출법에 비하여 미토콘드리아 DNA와 아밀로제닌 유전자 증폭 결과가 더 우수한 것으로 나타났다.

  • PDF

Alpers-Huttenlocher Syndrome First Presented with Hepatic Failure: Can Liver Transplantation Be Considered as Treatment Option?

  • Park, Sowon;Kang, Hoon-Chul;Lee, Jin-Sung;Park, Young Nyun;Kim, Seung;Koh, Hong
    • Pediatric Gastroenterology, Hepatology & Nutrition
    • /
    • 제20권4호
    • /
    • pp.259-262
    • /
    • 2017
  • Mitochondria play essential role in eukaryotic cells including in the oxidative phosphorylation and generation of adenosine triphosphate via the electron-transport chain. Therefore, defects in mitochondrial DNA (mtDNA) can result in mitochondrial dysfunction which leads to various mitochondrial disorders that may present with various neurologic and non-neurologic manifestations. Mutations in the nuclear gene polymerase gamma (POLG) are associated with mtDNA depletions, and Alpers-Huttenlocher syndrome is one of the most severe manifestations of POLG mutation characterized by the clinical triad of intractable seizures, psychomotor regression, and liver failure. The hepatic manifestation usually occurs late in the disease's course, but in some references, hepatitis was reportedly the first manifestation. Liver transplantation was considered contraindicated in Alpers-Huttenlocher syndrome due to its poor prognosis. We acknowledged a patient with the first manifestation of the disease being hepatic failure who eventually underwent liver transplantation, and whose neurological outcome improved after cocktail therapy.

Restriction and transcription maps of mitochondrial DNA of trimorphomyces papilionaceus

  • Jeoung, Won-Jin;Hong, Soon-Gyu;Won, Kang-Young;Jung, Hack-Sung
    • Journal of Microbiology
    • /
    • 제33권2호
    • /
    • pp.149-153
    • /
    • 1995
  • Mitochondrial DNA has been isolated from Trimorphomyces papilionaceus. By analyzing DNA fragments digested by restriction enzymes, a restriction site map has been constructured. The mtDNA of T. papilionaceus amounts to 48.5 kb in size and is circular in structure. Entire mitochondrial DNA was cloned in E coli plasmids and Northern blot hybridization was done using cloned and subcloned DNAs as probes. Based on hybridization results of mitochondrial RNA transcripts, a transcription map was prepared.

  • PDF

Chlamydomonas reinhardtii로부터 분리, 정제된 DNA Methyltransferase 활성에 대한 Polyamine의 영향 (Effect of Polyamines on purified DNA Methyltransferase from Chlamydomonas reinhardtii)

  • 이명민
    • Journal of Plant Biology
    • /
    • 제32권4호
    • /
    • pp.331-341
    • /
    • 1989
  • DNA methyltransferase was purified 282.6-fold from Chlamydomonas reinhardtii 21gr (mt+) gametic cell to examine the effect of polyamine on the enzyme acctivity. Polyacrylamide gel electrophoresis(PAGE) revealed at least three bands(1 major band, 2 minor bands). Among these, the major band represents DNA methyltransferase. Polyacrylamide gel electrophoresis in the presence of 0.1% sodium dodecylsulfate(SDS-PAGE) revealed a major band with M.W. 60,000. DNA methyltransferase activity was inhibited more effectively by spermine than by spermidine, and the inhibition by putrescine was smaller than spermine and spermidine. DNA methyltransferase activity was inhibited by 40% and 53% at 5mM and 20mM spermine, respectively. In the case of spermidine, the inhibition was 35% at 10mM and 44% at 20mM. However, the inhibition by putrescine appeared only above 5mM and reached about 25% at 20mM.

  • PDF

DNA Barcoding of Boccardiella hamata (Annelida: Polychaeta: Spionidae) in South Korea

  • Lee, Geon Hyeok;Yoon, Seong Myeong;Min, Gi-Sik
    • Animal Systematics, Evolution and Diversity
    • /
    • 제36권3호
    • /
    • pp.268-273
    • /
    • 2020
  • A spionid polychaete, Boccardiella hamata (Webster, 1879) has been found from mud in crevices between the shells of oysters and adherent substrates in South Korea. The sequences of mitochondrial DNA (mtDNA) cytochrome c oxidase subunit 1 (CO1), 16S ribosomal DNA (16S), and the nuclear 18S ribosomal DNA (18S) from Korean individuals of Boccardiella hamata were determined in the present study. The molecular analysis based on the 18S rRNA gene sequences showed clear separation among the spionid polychaete species, and the sequences of Korean and Japanese individuals are completely identical. The morphological diagnosis and photographs of B. hamata are also provided.