• 제목/요약/키워드: moutan cortex

검색결과 89건 처리시간 0.03초

고지방식이 유도 비만 마우스에서 창출, 지모, 육계, 목단피 혼합추출물의 항비만 및 항당뇨 효능 연구 (Antiobesity and Antidiabetic Effects of Polyherbal Extract with Atractylodis Rhizoma, Anemarrhenae Rhizoma, Cinnamomi Cortex, and Moutan Radicles Cortex in High Fat Diet-induced Obesity Mice)

  • 정수민;설영현;전가윤;박민하;유이;강석용;박용기;정효원
    • 한방비만학회지
    • /
    • 제20권2호
    • /
    • pp.69-77
    • /
    • 2020
  • Objectives: In this study, we investigated the antiobesity and antidiabetic effects of polyherbal extract, DM2 consisting of Atractylodis Rhizoma, Anemarrhenae Rhizoma, Cinnamomi Cortex, and Moutan Radicles Cortex in high fat diet-induced obesity mice. Methods: DM2 extract was prepared with a hot water. Six-week-old male C57BL/6N mice were fed a high-fat diet (HFD) for 8 weeks and then administrated with DM2 extract (500 mg/kg, p.o.) for 4 weeks. The changes of physiological markers, body weight (BW), food and water intakes, and the levels of fasting blood glucose (FBG) were measured once a week for 4 weeks in mice. The the serum levels of glucose, insulin, aspartate aminotransferase (AST), alanine aminotransferase (ALT), total cholesterol (T-CHO), triglyceride, and low density lipoprotein cholesterol in sera were measured in mice using autometic chemical analyzer and enzyme linked immunosorbant assay. We also observed the histological changes of liver and pancreatic tissues with Hematoxylin & Eosin staining. Results: In physiological change, the increases of BW, calorie intake, and FBG in HFD-induced obese mice were significantly decreased after administration of DM2 extract for 4 weeks. The decrease of water intake was significantly increased in DM2 extract-administrated mice. In serological change, the administration of DM2 extract in obesity mice was significantly decreased the serum levels of glucose, insulin, T-CHO, AST, and ALT levels. We also found that DM2 extract inhibited the increase of lipid droplets in liver and the structural destruction of pancreatic tissues in obesity mice. Conclusion: Our study demonstrated that DM2 extract has antiobesity antidiabetic effects with body weight loss, decrease of glucose and insulin levels, and lipid accumulation on liver tissue.

Synergistic Growth Inhibition of Herbal Plant Extract Combinations against Candida albicans

  • Jeemin YOON;Tae-Jong KIM
    • Journal of the Korean Wood Science and Technology
    • /
    • 제51권2호
    • /
    • pp.145-156
    • /
    • 2023
  • Many skin diseases are caused by microbial infections. Representative pathogenic fungus and bacterium that cause skin diseases are Candida albicans and Staphylococcus aureus, respectively. Malassezia pachydermatis is a fungus that causes animal skin diseases. In this study, we propose a method for removing pathogenic microorganisms from the skin using relatively safe edible herbal extracts. Herbal extracts were screened for skin health through the removal of pathogenic microorganisms, and combinations for effective utilization of the screened extracts were identified. In this study, among methanol extracts of 240 edible plants, C. albicans, S. aureus, and M. pachydermatis were killed by extracts of 10 plants: Acori Gramineri Rhizoma, Angelicae Tenuissimae Radix, Cinnamomi Cortex, Cinnamomi Ramulus, Impatientis Semen, Magnoliae Cortex, Moutan Cortex Radicis, Phellodendri Cortex, Scutellariae Radix, and Syzygii Flos. By evaluating the synergistic antifungal activities against C. albicans using all 45 possible combinations of these 10 extracts, five new synergistic antifungal combinations, Acori Gramineri Rhizoma with Magnoliae Cortex extracts, Acori Gramineri Rhizoma with Phellodendri Cortex extracts, Angelicae Tenuissimae Radix with Magnoliae Cortex extracts, Magnoliae Cortex with Phellodendri Cortex extracts, and Phellodendri Cortex with Syzygii Flos extracts, were identified. By utilizing the selected extracts and five combinations with synergistic antifungal effects, this work provides materials and methods to develop new and safe methods for treating candidiasis using natural products.

Isolation of Anti-Septic Shock Agents from Moutan Cortex

  • Li, Gao;Kim, Sung-Hwan;Xu, Ming-Lu;Kim, Jae-Hyun;Song, Dong-Keun;Son, Jong-Keun
    • 대한약학회:학술대회논문집
    • /
    • 대한약학회 2001년도 Proceedings of International Convention of the Pharmaceutical Society of Korea
    • /
    • pp.262.3-263
    • /
    • 2001
  • PDF

생약 제제가 세포활성도에 미치는 효과 (Effects of some herbal drugs on gingival fibroblast and periodontal ligament cellular activity)

  • 두진수;강정구;유형근;신형식
    • Journal of Periodontal and Implant Science
    • /
    • 제27권3호
    • /
    • pp.459-468
    • /
    • 1997
  • Healing of periodontal tissues require the migration and proliferation of gingival fibroblasts and periodontal ligament cells. There is many evidences that the some agents like cytokines and polypeptide growth factors are mediate these cellular events in wound healing. Recently someone is interested in herbal drugs on periodontal tissue healing processes. The purpose of this study was to examine the effects of 4 herbal drugs, Carthami Flis, Moutan Redias Cortex, Scirpi Rhisoma, Seed of Carthamus tinctorius L. on human gingival fibroblasts and periodontal ligament cells. Periodontal ligament cells and gingival fibroblasts were primarily cultured from extracted premolar with non-periodontal diseases. The powder from extracted. herbal drugs were prepared with distilled water. Cells were cultured with DMEM at $37^{\circ}C$, 5% $CO_2$, 100% humidity incubator, and treated with each herbal drugs with proper concentration for 1, 2, and 3 days. The cell activity was determined by ELISA reader using MTT assay. There was the most significant elevation in $10^{-3}g/ml$ of almost herbal drugs on cellular activities. The result of this study demonstrated that Carthami Flis, Moutan Radicis Cortex, Scirpi Rhisoma, Seed of Carthamus tinctorius L. appears to have beneficial effect on healing process after periodontal treatment.

  • PDF

목단피가 세포고사의 억제에 미치는 영향에 관한 연구 (Studies on the Anti-apoptotic Effect of the Mudanpi)

  • 권덕윤;배영춘;이상민;유관석;주종천;김경요
    • 동의생리병리학회지
    • /
    • 제18권4호
    • /
    • pp.1071-1077
    • /
    • 2004
  • Mudanpi (Cortex Moutan Radicis; the root cortex of Paeonia suffruticosa Andrews) is an important Chinese crude drug used in many oriental prescriptions. 1,2,3,4,6-Penta-O-galloyl-beta-D-glucose (PGG), a major component of this crude drug, has been shown to possess potent antioxidant, anti-mutagenic and anti-proliferative effects. In this study, I examined whether PGG could protect Neuro 2A cells, a kind of neuronal cell lines, from oxidative damage through the induction of HO-1 expression and HO activity. Exposure of Neuro 2A cells to PGG (10-50μM) resulted in a concentration- and time-dependent induction of HO-1 mRNA, and protein expressions and heme oxygenase activity. PGG protected the cells from hydrogen peroxide-induced cell death. The protective effect of PGG on hydrogen peroxide-induced cell death was abrogated by zinc protoporphyrin IX (ZnPP IX), a HO inhibitor. These results indicate that PGG is a potent inducer of HO-1 and HO-1 induction is responsible for the PGG-mediated cytoprotection against oxidative damage.

계지복령환(桂枝茯笭丸) 및 그 구성약물(構成藥物)의 혈소판응집억제(血小板凝集抑制)에 관(關)한 연구(硏究) (Effect of Geijibokryunghwan and each constituent herb on inhibition of platelet aggregation)

  • 김종구;박선동;박원환
    • 동국한의학연구소논문집
    • /
    • 제8권2호
    • /
    • pp.115-129
    • /
    • 2000
  • 동물성(動物性) 지방섭취량(脂肪攝取量)의 증가(增加), 운동부족(運動不足), 비만(肥滿), 스트레스의 가중(加重), 고령화(高齡化)의 증가(增加) 등(等)의 원인(原因)으로 순환기계질환(循環器系疾患)의 발병률(發病率)이 증가(增加)하고 있으며, 이러한 순환기계질환(循環器系疾患)의 위험인자(危險因子)로서 혈전증(血栓症)이 중요(重要)하게 대두되고 있다. 특히 최근 문제시되고 있는 협심증(狹心症)이나 심근경새(心筋梗塞)등의 허혈성(虛血性) 심질환(心疾患)은 혈소판응집(血小板凝集)에 의해 일어나는 혈전형성(血栓形成)에 기인(起因)하고 있다. 한의학(韓醫學)에서 혈전증(血栓症)은 어혈(瘀血)의 범주(範疇)에 속(屬)하며, 어혈(瘀血)은 각종 병리적(病理的) 원인(原因)에 의해 발생한 전신성(全身性) 또는 국소성(局所性)의 혈액순환(血液循環) 장애(障碍) 또는 혈류정체(血流停滯)와 그에 수반되는 일련의 증후(症候)를 나타내며, 경계정충, 고창(鼓脹), 적취(積聚), 미하, 전광(癲狂), 중풍등(中風等)의 발병원인(發病原因)이 된다. 또한 어혈(瘀血)에 의한 각종 증후(症候)에는 활혈거어제(活血祛瘀劑) 또는 구어혈제(驅瘀血劑)등이 사용되고 있다. 본(本) 연구(硏究)에서는 한의학(韓醫學)에서 어혈증(瘀血症)으로 야기(惹起)되는 여러 가지 증상(症狀)의 개선에 사용되는 구어혈제(驅瘀血劑)들의 혈소판응집(血小板凝集)에 미치는 영향을 검색하기 위하여 계지복령환(Geijibokryunghwan; GBH) 및 그 구성약물(構成藥物)을 사용(使用)하였다. 계지복령환은 "금궤요략" 에 있는 방(方)으로써 거사부상정(祛邪不傷正)하고 조기한열(調氣寒熱)하여 예로부터 구어혈제(驅瘀血劑)로 사용되어 왔다. 이에 계지복령환 및 그 구성약제(構成藥劑)의 ADP, AA 또는 collagen으로 유도되는 혈소판응집(血小板凝集)에 대하여 억제효과(抑制效果)를 탐색(探索)한 결과(結果), 계지복령환 및 개별(個別) 구성약물(構成藥物)의 혈소판응집억제작용(血小板凝集抑制作用)을 확인하였고, 혈소판응집(血小板凝集)으로 야기(惹起)되는 혈전증(血栓症)등에 계지복령환 및 개별(個別) 구성약물(構成藥物)은 매우 임상실험적(臨床實驗的) 응용가치(應用價値)가 있는 것으로 생각되었다.

  • PDF

항균작용을 가진 수종 한약재의 구취억제 효과 (Deodorizing Effect of Several Antibacterial Medicinal Herbs on Oral Malodor)

  • 김현경;박재우;윤성우;류봉하;김진성
    • 대한한의학회지
    • /
    • 제31권4호
    • /
    • pp.151-163
    • /
    • 2010
  • Objective: We investigated the oral malodor inhibitory effect of Scutellariae Radix (SR), Phellodendri Cortex (PC), Moutan Cortex (MTC) and Magnoliae Cortex (MGC) for the development of a gargle solution. Methods: 1. Against P. gingivalis and Pr. intermedia, the minimal bactericidal concentration (MBC) and the change of viable cells that were exposed to 1% each herbal extract were observed. 2. Deodorizing activity of 2% herbal extract and Garglin $Mint^{(R)}$ against methyl mercaptan were evaluated by gas chromatography (GC). 3. We used the salivary sediment system (SSS) as the malodor model. 4. The clinical examination was repeated 3 times by 2 subjects by $Halimeter^{(R)}$. Baseline VSC of each subject was measured. Then, the control subject gargled with cysteine for 30 sec. After 4 min, subjects would gargle for 30 seconds with herbal extracts (2%) and Garglin $Mint^{(R)}$. Subsequently, the concentration of VSC was measured at 0, 4, 8, 12, 16, 20, 40 and 60 minutes. Results: 1. Against P. gingivalis, MBC of SR, PC and MTC was 0.1%, and MBC of MGC was 1%. Removal time of P. gingivalis was as follows; 5 hr in MGC, 24 hr in SR and PC, and 48 hr in MTC. Against Pr. intermedia, MBC of SR and PC was 0.5%, and MBC of MTC, MGC was 1%. Removal time of Pr. intermedia was as follows; 5 hr in MTC and 24 hr in SR, PC and MGC. 2. Deodorizing effect of herbal extracts against methyl mercaptan was as follows; MGC and MTC had 100%, SR had 82.22%, PC had 66.60%, Garglin $Mint^{(R)}$ had 40.54%. 3. In the experiment using SSS, PC and MTC had statistically significant malodor-inhibitory effects (p<.05). 4. In the clinical examination, PC and MGC had statistically significant inhibitory effects at every elapsed time compared to the control subject. MTC had that until 40 min. SR had that at 0, 4, 8, 20, and 60 min. Conclusions: SR, PC, MTC and MGC have an antibacterial effect and the chemical removable activity of the oral malodor caused by VSC. These four herbs could have potential as effective anti-malodor agents.

생약재 추출물의 Staphylococcus aureus 와 Escherichia coli O157:H7에 대한 항균효과 (Antimicrobial Effects of Herbal Medicine Extracts on Staphylococcus aureus and Escherichia coli O157:H7)

  • 채화;최수임;이윤미;허태련
    • KSBB Journal
    • /
    • 제17권6호
    • /
    • pp.537-542
    • /
    • 2002
  • 50가지 생약재를 95% 에탄올과 물추출하여 식중독 세균인 S. aureus와 E. coli O157:H7에 대한 항균활성을 조사하였다. 한천배지확산법에 따른 생육저해효과를 측한 결과, S. aureus 에 대하여 우슬>목단피>지실>황백, 건강, 민들레 순으로, E. coli O157:H7에 대하여 목단피>우슬>건강>지실, 황백 순으로 활성을 나타내었다. MIC는 S. aureus에 대하여 우슬이 156.25 $\mu$g/mL, 목단피와 황백이 625 $\mu$g/mL로 나타났으며, E. coli O157:H7에 대하여 우슬과 목단피의 MIC는 각각 625, 312.5 $\mu$g/mL로 높은 항균력을 나타내었다. 가장 높은 활성을 보인 우슬과 목단피를 용매분획(hexane, CHC1$_3$, ethyl acetate, butanol)하여 한천배지 확산법에 의한 두 균에 대한 생장저해 효과를 측정하였다. 우슬은 ethyl acetate 분획물에서, 목단피는 CHC1$_3$과 ethyl acetate 분획물에서 각각 높은 활성을 나타내었다.

목단피의 세포독성 물질 (A Cytotoxic Compound from Moutan Cortex Radicis)

  • 주보연;김문일;최수라;명평근;성연희;송경식;배기환
    • 한국약용작물학회지
    • /
    • 제12권3호
    • /
    • pp.255-261
    • /
    • 2004
  • 21 종의 생약 추출물을 선정하여 $5\;{\mu}g/ml$의 농도에서 SK-MEL-28 cell 에 대한 세포독성을 조사한 결과 목단피의 메탄올 추출물이 74.3%의 성장율을 보였다. 활성 물질을 찾기 위하여 목단피로부터 silica gel column chromatography를 실시하여 hexane 분획과 EtOAc 분획에서 충 5개의 화합물을 분리하였고 $mp,\;UV,\;IR,\;^1H-NMR,\;^{13}C-NMR$ 등 각종 물리, 화학적 data로부터 그 구조를 paeonol (1), benzoylpaeoniflorin (2), benzoic acid (3), 2,5-dihydroxy-4-methoxy-acetophenone (4), paeoniflorin (5)으로 동정하였다. 분리한 물질을 human 피부 암세포인 SK-MEL-28 세포주에 대하여 $10\;{\mu}g/ml$의 농도에서 SRB방법으로 세포독성을 측정한 결과 compound 4가 $ED_50$ 값이 $5.92\;{\mu}g/ml$로 가장 좋은 세포독성을 나타내었다. 이결과는 compound 4가 SK-MEL-28 melanoma 세포주에 대한 새로운 항암 후보 물질임을 제시한다.