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Studies on the Anti-apoptotic Effect of the Mudanpi  

Kwon Duck Yun (Department of Sasang Constitutional Medicine, College of Oriental Medicine, Wonkwang University)
Bae Young Chun (Department of Sasang Constitutional Medicine, College of Oriental Medicine, Wonkwang University)
Lee Sang Min (Department of Sasang Constitutional Medicine, College of Oriental Medicine, Wonkwang University)
Yoo Kwan Seok (Department of Sasang Constitutional Medicine, College of Oriental Medicine, Wonkwang University)
Joo Jong Cheon (Department of Sasang Constitutional Medicine, College of Oriental Medicine, Wonkwang University)
Kim Kyung Yo (Department of Sasang Constitutional Medicine, College of Oriental Medicine, Wonkwang University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.18, no.4, 2004 , pp. 1071-1077 More about this Journal
Abstract
Mudanpi (Cortex Moutan Radicis; the root cortex of Paeonia suffruticosa Andrews) is an important Chinese crude drug used in many oriental prescriptions. 1,2,3,4,6-Penta-O-galloyl-beta-D-glucose (PGG), a major component of this crude drug, has been shown to possess potent antioxidant, anti-mutagenic and anti-proliferative effects. In this study, I examined whether PGG could protect Neuro 2A cells, a kind of neuronal cell lines, from oxidative damage through the induction of HO-1 expression and HO activity. Exposure of Neuro 2A cells to PGG (10-50μM) resulted in a concentration- and time-dependent induction of HO-1 mRNA, and protein expressions and heme oxygenase activity. PGG protected the cells from hydrogen peroxide-induced cell death. The protective effect of PGG on hydrogen peroxide-induced cell death was abrogated by zinc protoporphyrin IX (ZnPP IX), a HO inhibitor. These results indicate that PGG is a potent inducer of HO-1 and HO-1 induction is responsible for the PGG-mediated cytoprotection against oxidative damage.
Keywords
Mudanpi(Cortex Moutan Radicis); anti-apoptotic effect; PGG; Neuro 2A cell;
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