• 한국식품과학회지
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• 제31권5호
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• pp.1203-1210
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• 1999

더덕, 곰취, 컴프리를 사용하여, 총질소함량을 기준으로 소맥대신 이들 산채류를 일정비율로 대체하여 더덕 및 산채혼합 간장을 제조하여 그 특성을 살펴보았다. 제조된 간장의 총 질소함량은 일반적으로 산채 대체량의 증가에 따라 증가하였다. 숙성 4개월 후의 무기질 함량에 있어 더덕 20% 대체간장은 Ca, K 함량이 각각 1.3배, 1.5배의 증가율을 나타내었으며, 산채혼합 10% 대체간장도 Ca 함량이 1.5배 증가하였다. 대체량 별로 아미노산 함량 변화를 살펴보면 더덕간장 10% 대체군의 경우 threonine과 aspartic acid가, 산채혼합 10% 대체군은 tyrosine과 arginine이 높은 증가율를 나타내었다. Rec assay system을 이용한 항돌연변이 시험에서, 더덕간장은 10%와 20% 대체군이, 산채혼합 간장은 7%와 10% 대체군이 다른 시료에 비해 높은 항돌연변이성을 나타내었다. 관능검사 결과. 전체적 기호도에 있어 더덕간장은 7% 대체군, 산채혼합 간장은 5% 대체군이 가장 우수하였다.

• Applied Biological Chemistry
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• 제31권1호
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• pp.38-45
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• 1988

10종류(種類)의 산채류가열즙(山菜類加熱汁)에 대한 돌연변이원성(突然變異原性)을 Spore rec-assay, Ames test 그리고 DNA 절단실험(切斷實驗)으로 검토(檢討)하였으며 가열즙(加熱汁)의 조제(調製)는 $100^{\circ}C$에서 20분간 가열하여 가열즙(加熱汁)을 얻었다. Spore rec-assay 결과 10종류(種類)의 시료(試料)모두 변이원성(變異原性)은 없었으며 금속이온의 영향에서 소리쟁이가열즙은 $Pb^{2+}$, 곰취와 참나물가열즙의 경우는 $Zn^{2+}$의 존재하에서 변이원성을 나타내었다. Ames 시험과 DNA 절단시험에서도 시료모두 변이원성은 없었으며 DNA 절단(切斷)시험에서는 곰취, 머위, 잔대, 개미취가열즙의 경우는 25mM $Cu^{2+}$ 공존하에서 절단활성(切斷活性)이 촉진되었다. 그러나 두릅, 원추리, 잔대, 참나물 그리고 참취가열즙(加熱汁)은 $Fe^{2+}$의 공존시 DNA 절단활성(切斷活性)을 억제(抑制)시키는 결과(結果)를 얻었다. Benzo${\alpha}$pyrene에 대한 돌연변이(突然變異) 억제작용(抑制作用)에서는 시료(試料) 모두 농도증가(濃度增加)에 따라 강(强)한 변이원(變異原) 억제작용(抑制作用)을 나타내었다.

• 한국식품영양과학회지
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• 제27권6호
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• pp.1177-1182
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• 1998

The antimutagenic and cancer cell growth inhibitory effects of woorimil contained herb and seaweed powders were examined. While woorimil itself showed only 40% antimutagenic effect on S. typhymurium TA98 against 4NQO(0.15 g/plate), water extracts of mountain herbs and seweeds including Comfrey, wormwood, Kale, Angelica utilis and pine leaves showed 80~90% antimutagenicity. On the other hand, these extracts along with woorimil showed 68 to 80% antimutagenic activities. Low antimutagenic activities of less than 50% were shown when these extracts were tested on TA98 against Trp P 1(0.5 g/plate), but high antimutagenic activities of 80~93.3% were shown on TA100. Water extracts of Capsella bursa pastoris and Allium grayi exhibited 60~80% of the activites in cytotoxicity tests of woorimil water extracts(0.5mg/ml) on human lung carcinoma cell. A549 showed 10% cell growth inhibitory effect. When mixed with Comfrey and Angelica utilis extracts, it showed 23~25% inhibition and other extracts showed only 12~23% inhibition. Cytotoxicity test of woorimil extracts on human liver cancer cell Hep3B revealed 20% inhibition. The additions of pine needle extracts, Angelica utilis and Comfrey showed 33%, 29% and 25% inhibition, respectively. But other extracts showed only 20% inhibition.

• Journal of Ginseng Research
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• 제34권1호
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• pp.41-46
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• 2010

Expensive herbs such as ginseng are always a possible target for fraudulent labeling. New mountain ginseng strains have occasionally been found deep within mountain areas and commercially traded at exorbitant prices. However, until now, no scientific basis has existed to distinguish such ginseng from commonly cultivated ginseng species other than by virtue of being found within deep mountain areas. Polymerase chain reaction (PCR) analysis of the internal transcribed spacer has been shown to be an appropriate method for the identification of the most popular species (Panax ginseng) in the Panax ginseng genus. A single nucleotide polymorphism (SNP) has been identified between three newly found mountain ginseng (KGD4, KGD5, and KW1) and already established Panax species. Specific PCR primers were designed from this SNP site within the sequence data and used to detect the mountain ginseng strains via multiplex PCR. The established multiplex-PCR method for the simultaneous detection of newly found mountain ginseng strains, Korean ginseng, and foreign ginseng in a single reaction was determined to be effective. This study is the first report of scientific discrimination of "mountain ginsengs" and describes an effective method of identification for fraud prevention and for uncovering the possible presence of other, cheaper ginseng species on the market.

• 동의생리병리학회지
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• 제17권1호
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• pp.203-208
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• 2003

Antioxidant effects of Rehmannia vaporata, Discorea Radix, Corni Fructus, Hoolen, Alismatis Radix, and Mountain Cortex Radicis composing Yukmijihwang-tang were studied. The results are as follows; 1. As a result of detecting the defensive effect of each component on cell damage, only the survival rate of cells with 10 ㎎/㎖ Mountain Cortex Radicis was significantly increased. 2. Next, we examined the inhibitory effects of them on ROS occurrence. The result showed significant inhibition of ROS occurrence in cells; with 10 ㎎/㎖ Rehmannia vaporata, cells with 10 ㎎/㎖ Corni Fructus, and cells with 10 ㎎/㎖ Mountain Cortex Radicis. Since the cells with 10 ㎎/㎖ Rehmannia vaporata, however, showed significant cypotoxicity, its result is not meaningful. 3. Finally, the investigation of ROS occurrence / cell found that Corni Fructus and Mountain Cortex Radicis had significant inhibitory effect on ROS occurrence.

• 한국식품저장유통학회지
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• 제6권1호
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• pp.48-54
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• 1999

The effect of irradiation of heat treatment either alone or in combination on the shelf-life of mixed mountain edible herb drinks (MMEHD) was investigated during storage. The MMEHD made from fresh Spuriopinella bracycarpar, Ligularia fischeri and Aster scaber was gamma -irradiated at doses of 0.1 kGy to 1 kGy. Microbial population, color change, vitamin C content, and sensory quality were evaluated during storage at 4, 25 and 35oC. Heated MMEHD induced the growth of total counts, mold and yeast as compared to the non-heated MMEHD. While some reduction in the microbial growth was observed in 1kGy-irradiated groups of both with or without heating. In both groups, L and b values decreased, but a value increased during storage. Also, heating drinks showed increased L and b values and reduced a value, compared to the non-heating drinks. Non-heated drinks showed 82% loss of vitamin C, whereas 25% loss of vitamin C was occurred in the irradiated drinks during storage at 4oC for 4days. In the meanwhile, non-irradiated heating drinks showed 99% loss of vitamin C, but irradiated heating drinks showed 58-65% reduction of vitamin C. Non-irradiated drinks without heating showed more bright color than irradiated ones, but irradiated drinks showed more enhanced brightness during storage. Also, irradiated drinks showed better falvor, sensory quality, and overall acceptability than non-irradiated drinks.

• 한국산림과학회지
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• 제95권3호
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• pp.282-290
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• 2006

울릉도 산림식생의 유형은 총 232개 조사구를 토대로 식물사회학과 TWINSPAN분석에 의해 구분되었으며, 유형별 생활형조성이 분석되었다. 이 지역의 산림식생유형은 모두 22개 식생단위로 나타났으며, 크게 산지식생과 해안식생의 2개 광역그룹으로 대별되고, 전자는 4개 군락, 11개 군, 7개 소군, 후자는 2개 군락, 5개 군의 체계를 나타내었다. 산지식생과 해안식생의 생활형조성 특성을 보면 전자는 교목성과 초본성식물, 반지중식물, 바람에 의한 산포와 자연낙하기관형, 직립형식물 등의 구성비가 상대적으로 높았고, 후자는 관목성식물, 지표식물, 바람과 동물에 의한 산포기관형, 좁은 범위에 연락체를 만드는 유형과 포복경식물의 구성비가 높았다. 특히 산지식생형의 하위단위에 속하는 말오줌나무군락(A6)은 교란의 영향으로 바람에 의한 산포기관형, 약간 넓은 범위에 연락체를 만드는 유형과 덩굴형의 식물의 구성비가 높게 나타났다.

• 대한본초학회지
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• 제32권2호
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• pp.17-24
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• 2017

Objective : This study was designed to evaluate candidate materials as anti-inflammation agent from extracts of various Korean Compositae herbs in Hwaak mountain. Among Korea medicinal herbs, Ainsliaea acerifolia (AA) belongs to the Compositae family, has been used for the treatment of rheumatic arthritis. However, AA has not been previously reported to have an anti-inflammatory effect. Therefore, we investigated the anti-inflammatory effects of AA and its underlying molecular mechanisms in lipopolysaccharide (LPS)-induced RAW 264.7 macrophages. Methods : Cell viability was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay in RAW 264.7 macrophages. Nitric oxide (NO) was measured with Griess reagent and pro-inflammatory cytokines were detected by enzyme immunoassay (EIA) kits in LPS-stimulated RAW 264.7 macrophages. Protein expressions of inducible nitric oxide synthase, and cyclooxygenase-2 (COX-2) and p65 subunit of nuclear $factor-{\kappa}B$ ($NF-{\kappa}B$) were determined by Western blot analysis. Results : Among 8 extracts of Korean Compositae herbs tested, AA showed the inhibition of NO production without cytotoxicity. Consistent with the observation, AA reduced the expression levels of iNOS and COX-2 proteins in LPS-simulated RAW 264.7 macrophages in dose-dependent manner. In addition, AA inhibited the productions of $TNF-{\alpha}$ and IL-6 in LPS-simulated RAW 264.7 macrophages. However, AA did not inhibit activation of p65 $NF-{\kappa}B$ in LPS-simulated RAW 264.7 macrophages. Conclusion : These results suggest that down-regulation of iNOS, COX-2 protein expression and $TNF-{\alpha}$ and IL-6 production by AA are responsible for its anti-inflammatory effects.

• 한국자원식물학회지
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• 제18권1호
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• pp.117-122
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• 2005

본 연구는 산림의 유용한 식물중 하나인 멸가치의 생육특성을 밝혀 보고자 습도와 광도를 조사하였다. 주요 산채류중 하나인 멸가치의 생약성분이 정신분열증을 일으키는 dopamine함량 감소작용을 일으켰다는 보고 등 자원으로서의 가치가 큰 것으로 나타났다. 이러한 멸가치의 생육에 가장 큰 영향을 주는 것은 습도로 $50\%$ 이상이 좋은 것으로 나타났다. 또한 온도변화가 자고 광량은 $200\;{\mu}mol\;s^{-1}m^{-2}$이하의 곳이 생육에 적합한 장소로 분석되었다. 이 연구 결과 음지식물의 증식에 있어서 습도를 $50\%$이상 유지하는 것이 중요한 것으로 나타났다.

• 한국산림과학회지
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• 제104권3호
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• pp.495-502
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• 2015

본 연구는 주요 약초류(8개 품목) 재배에 투입된 비용과 수입 등을 파악하여 소득분석을 실시함으로써 향후 단기소득임산물 손실에 대한 보상 기준 및 임업경영환경 개선을 위한 정책수립에 필요한 기초자료 제공을 목적으로 수행하였다. 약초류 재배에 대한 소득분석(10a 기준) 결과, 단위면적당 연평균소득은 잔대가 14,233천원으로 가장 높았고, 다음으로 하수오 4,121천원, 천마 3,766천원, 삼지구엽초 3,537천원, 삽주 2,655천원, 독활 1,048천원, 작약 1,025천원, 시호 919천원 등으로 나타났다. 이는 2014년에 분석한 주요 수실류 소득과 비교하면 약초류의 단위면적당 연평균소득이 비교적 높은 것으로 나타났다. 특히, 잔대와 독활의 경우 새순은 식용으로 뿌리는 약용으로 이용되고 있어 향후 농산촌지역의 새로운 소득원으로 부상할 수 있을 것으로 판단된다. 한편, 작약은 중국산으로 인한 판매 가격하락을 보이고 있어 향후 한-중 FTA가 시행되면 타 약초류에도 중국산으로 인한 소득손실 피해가 예상된다.