• BMB Reports
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• v.50 no.10
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• pp.522-527
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• 2017

A large number of transcriptional activation domains (TADs) are intrinsically unstructured, meaning they are devoid of a three-dimensional structure. The fact that these TADs are transcriptionally active without forming a 3-D structure raises the question of what features in these domains enable them to function. One of two TADs in human glucocorticoid receptor (hGR) is located at its N-terminus and is responsible for ~70% of the transcriptional activity of hGR. This 58-residue intrinsically-disordered TAD, named tau1c in an earlier study, was shown to form three helices under trifluoroethanol, which might be important for its activity. We carried out heteronuclear multi-dimensional NMR experiments on hGR tau1c in a more physiological aqueous buffer solution and found that it forms three helices that are ~30% pre-populated. Since pre-populated helices in several TADs were shown to be key elements for transcriptional activity, the three pre-formed helices in hGR tau1c delineated in this study should be critical determinants of the transcriptional activity of hGR. The presence of pre-structured helices in hGR tau1c strongly suggests that the existence of pre-structured motifs in target-unbound TADs is a very broad phenomenon.

• BMB Reports
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• v.50 no.10
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• pp.485-486
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• 2017

Many intrinsically unstructured/unfolded proteins (IUPs) contain transient local secondary structures even though they are "unstructured" in a tertiary sense. These local secondary structures are named "pre-structured motifs (PreSMos)" and in fact are the specificity determinants for IUP-target binding, i.e., the active sites in IUPs. Using high-resolution NMR we have delineated a PreSMo active site in the intrinsically unfolded mid-domain (residues 201-300) of SUMO-specific protease 4 (SUSP4). This 29-residue motif which we termed a p53 rescue motif can protect p53 from mdm2 quenching by binding to the p53-helix binding pocket in mdm2(3-109). Our work demonstrates that the PreSMo approach is quite effective in providing a structural rationale for interactions of p53-mdm2-SUSP4 and opens a novel avenue for designing mdm2-inhibiting anticancer compounds.

• Journal of the Korean Society of Costume
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• v.66 no.2
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• pp.15-31
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• 2016

Flower images are used as a design motif in various fields. Flower printings in clothes, in particular, usually represent nature. This study sets out to identify the characteristics of different fashion styles with flower printings, as well as the preferences and emotional images of Korean women in their 20s. The flower printings used in fashion design were classified into 5 types of styles: Modern, Natural Romantic, Maximalism, Neo-Hippie, and Ethnic style. Literature review and survey were conducted to identify the emotional images associated with the flower printings, as well as women's preferences. Through literature review, this study noted the formative elements of flower printings and their characteristics, as expressed in fashion designs. Then, the different styles were classified in order to provide theoretical foundation for the survey. The results of the study were significant in that they contributed to the definition and academic systemization of the characteristics of fashion styles with flower printings. Moreover, the study opened up possibilities for utilizing flowers to express a greater variety of meanings and influences in fashion. The findings can be used to enable fashion styles and emotional influences to be expressed through designs using natural motifs besides flowers.

• Fashion & Textile Research Journal
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• v.19 no.4
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• pp.373-384
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• 2017

This research has its significance on contributing to the establishment of sustainable fashion industry ecosystem of Seongsu area based on the creativity which is the driving force of economic growth in the 21st century. This study reviewed the major activities and processes of Seongsu-dong's regional specialized industries project and the social economic organizations's creation of fashion clusters by using specialized books, previous research, press releases such as newspapers, magazines, and the specialized internet site(www.seoul.go.kr). Also street casual-style clothes were designed based on work-wear that themed Seongsu-dong's industrial scene and social problems. The results of this study are as follows. Seongsu-dong represents the political and economic characteristics of a typical semi-industrial area in which the automobile repair, printing, textile, leather, and handmade industries are concentrated in accordance with the government's business and policy, and shows the technical characteristics through the internal complete industrial ecosystem integration of the handmade industries. In addition, social and cultural characteristics such as various local activities based on creativity are shown by social enterprises, and cultural artists. Based on the results of analyzing the regional characteristics of Seongsu-dong according to political, economic, technological, socio-cultural characteristics, eight fashion designs were made as motifs reflecting the regional characteristics of Seongsu-dong.

• The Research Journal of the Costume Culture
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• v.15 no.1 s.66
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• pp.68-80
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• 2007

Surrealism's method of expression and inspiration has been a continuous influence on fashion. What is important is how to identify and research a source of inspiration, and how to use this inspiration to guide the designs that can be worn on a real human body. Therefore, the purpose of this study was to show how to develop ideas and designs from Surrealist objects and how to contribute to the development of designs to the diversity in visual and functional effects along with the changes in consumer's interest. Documentary studies about Surrealism and Surrealist-inflected fashion applying the works of Surrealist and fashion images as a tool of historical research were proceeded. And the ideas for theme, the motifs for print patterns, and the concept of designs were borrowed from Surrealist objects touching on the imagery between the real and reflected, optical and artificial, and the designs were developed with several different visual and functional effects changing color, pattern, size, and length with the function of zippers in each item. It also gives the person wearing it amusement because it includes the concept of play and D.I.Y. With effective planning, it is possible to attract consumer's interest in the market.

• BMB Reports
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• v.39 no.6
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• pp.788-793
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• 2006

Bacterial DNA containing immunostimulatory CpG motifs can stimulate antigen-presenting cells to express co-stimulatory molecules and to produce various cytokines in vivo and in vitro. In this study, we fragmented macromolecular E.coli genomic DNA with DNase I, and analyzed the ability of the resulting DNA fragments to induce the NF-${\kappa}B$ activation and humoral immune response. Furthermore, using computational analysis and luciferase assay for synthetic ODNs based on the sequence of the immunostimulatory DNA fragments (DF-ODNs), an active component of DF-ODNs sequences was investigated. Experimental results demonstrated that DF-ODN is optimal for the NF-${\kappa}B$-responsive promoter activation in the mouse macrophage cell line and the humoral immune response in vivo. In agreement with the activity of the DF-ODNs processed by DNase I, a synthetic ODN based on the DF-ODN sequences is potent at inducing IL-12 mRNA expression in primary dendritic cells. These results suggest that the discovery and characterization of a highly active natural CpG-ODN may be achieved by the analyses of bacterial DNA fragments generated by a nuclease activity.

• BMB Reports
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• v.49 no.5
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• pp.282-287
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• 2016

Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) is a homo-trimeric cytotoxic ligand. Several studies have demonstrated that incorporation of artificial trimerization motifs into the TRAIL protein leads to the enhancement of biological activity. Here, we show that linkage of the isoleucine zipper hexamerization motif to the N-terminus of TRAIL, referred as ILz(6):TRAIL, leads to multimerization of its trimeric form, which has higher cytotoxic activity compared to its native state. Size exclusion chromatography of ILz(6):TRAIL revealed possible existence of various forms such as trimeric, hexameric, and multimeric (possibly containing one-, two-, and multi-units of trimeric TRAIL, respectively). Increased number of multimerized ILz(6):TRAIL units corresponded with enhanced cytotoxic activity. Further, a high degree of ILz(6):TRAIL multimerization triggered rapid signaling events such as activation of caspases, tBid generation, and chromatin condensation. Taken together, these results indicate that multimerization of TRAIL significantly enhances its cytotoxic activity.

• Journal of the Korean Society of Costume
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• v.54 no.6
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• pp.13-23
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• 2004

To date, Italian fashion rans the top even its rise is far behind Paris and New York. The purpose of this study is to analyze and compare the aesthetic characteristics of the italian fashion through two representative designers who contributed to italian fashion. Italy had unique industrial system and craftsmanship from the history and sense of the beauty and the uniqueness from the culture. After the World War II, Italy started to produce elegant sportswears influenced by the progressive manufacturing techniques and practical culture of America. Italian fashion of 'easy elegance' is spearheaded by Vesace and Armani. The aesthetic characteristics of Italian fashion which are shown in the artworks of them are as follows. First, the characteristics of Versace dated from southern sensibility are sensualism, expressionism, historicism. 1) Sensualism: tight silhouette, overexposure, glamorous form, brilliant color, fabric showing femininity, gaudy accessaries 2) Expressionism: brilliant print and color, trimmings and accessaries 3) Historicism: form and motifs that are shown in the historical costume Second, the characteristics of Armani succeeded from northern tailoring are purism, functionalism, and multi-culturism. 1) Purism: clean lines, architectural and geometric forms, natural body line, neutral tones, luxurious fabric 2) Functionalism: functional form, neutral tone, natural fabrics 3) Multi-culturism: design and motif inferred from ethnic costume As such. competition between two different groups made it possible that Italian fashion occupies a special place in the international fashion system.

• Korean Institute of Interior Design Journal
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• v.19 no.3
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• pp.86-94
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• 2010

Art-Wall is the term for a surface wall representing artistic expression by using different materials or images in interior spaces. Usually this is the focal point in an interior space and sometimes it called as an image wall. In the new millenium Art-walls began to use in living spaces. While in the early stages of the development, Art-Wall was limited in a living room, in contemporary it spread into overall living spaces using various patterns and materials. Therefore, the research on Art-Walls in contemporary living spaces is essential to understand this development. The purpose of this study is to find out characteristics of patterns on Art-Walls as an imagery element in contemporary interior spaces. To analyze the characteristic, the analysis table for Art-Walls are constructed based on classifications of wallpapers and textiles. Based on this analysis table, 89 examples of Art-Walls from four major periodicals from 2008 to 2009 were examined. As a result of the analysis, in contemporary interior spaces solid-texture pattern is most frequently appeared. Then natural motifs are the next most popular using oversized or irregular layouts. The result shows that naturalistic expression is significant in interior space in 2008 to 2009. Therefore, Art-Walls that adapted diverse patterns are an essential element in contemporary interiors to represent the major concept of the space.

• Fisheries and Aquatic Sciences
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• v.14 no.4
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• pp.303-310
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• 2011

We characterized a cytoplasmic actin gene locus in greenling Hexagrammos otakii (Scorpaeniformes). Genomic clones isolated from the greenling DNA library contained two homologous cytoplasmic actin gene copies (HObact2.1 and HObact2.2) in a tail-to-head orientation. Their gene structure is characterized by six translated exons and one non-translated exon. Exon-intron organization and the nucleotide sequences of the two actin gene isoforms are very similar. However, only the HObact2.1 isoform contains microsatellite-like, dinucleotide repeats in the 5'-flanking region (named HOms2002) and intron 1 following the non-translated exon 1 (named HOms769). One microsatellite locus (HOms769) was highly polymorphic while the other (HOms2002) was not. Based on bioinformatic analysis, different transcription factor binding motifs are related to stress and immune responses in the two actin isoforms. Semiquantitative and real-time reverse transcription-PCR assays showed that both isoform transcripts were detectable ubiquitously in all the tissues examined. However, the basal expression levels of each isoform varied across tissues. Overall, the two isoforms showed a similar, but not identical, expression pattern. Our data suggest that the cytoplasmic actin genes may be the result of a recent duplication event in the greenling genome, which has not experienced significant subfunctionalization in their housekeeping roles.